Accumulation of D6-unsaturated fatty acids in transgenic tobacco
... of a B. officinalis D6-fatty acid desaturase in transgenic tobacco plants results in the accumulation of D6-unsaturated fatty acids in all the tissues examined, consistent with the use of the ‘constitutive’ viral 35S promoter. Accumulation patterns in the c2 lines are similar to those in borage, bot ...
... of a B. officinalis D6-fatty acid desaturase in transgenic tobacco plants results in the accumulation of D6-unsaturated fatty acids in all the tissues examined, consistent with the use of the ‘constitutive’ viral 35S promoter. Accumulation patterns in the c2 lines are similar to those in borage, bot ...
IndelFR: a database of indels in protein structures and their flanking
... evolutionary history (9,10). However, there are few studies of the sequence–structure–function relationships of indels. Indels usually occur as reverse turns or coils within loops in a domain, most often on the surface of proteins (11–13). Compared to deletions, a succession of insertions and rapid ...
... evolutionary history (9,10). However, there are few studies of the sequence–structure–function relationships of indels. Indels usually occur as reverse turns or coils within loops in a domain, most often on the surface of proteins (11–13). Compared to deletions, a succession of insertions and rapid ...
embor2011116-sup-0001
... formation (elongation phase) of polypeptide chains from fully or partially unfolded states under different conditions and without any experimental information on the regions most sensitive to aggregation. It accounts for extrinsic factors such as pH, ionic strength and peptide concentration, and rep ...
... formation (elongation phase) of polypeptide chains from fully or partially unfolded states under different conditions and without any experimental information on the regions most sensitive to aggregation. It accounts for extrinsic factors such as pH, ionic strength and peptide concentration, and rep ...
Flavors of Protein Disorder
... Although proteins that fail to self-fold into specific 3-D structure are receiving increased attention as evidenced by several recent major reviews1-6, the concept is not new. That a native protein’s function can depend on a structural ensemble rather than a unique 3-D structure was suggested more t ...
... Although proteins that fail to self-fold into specific 3-D structure are receiving increased attention as evidenced by several recent major reviews1-6, the concept is not new. That a native protein’s function can depend on a structural ensemble rather than a unique 3-D structure was suggested more t ...
Application of small interfering RNAs modified by unlocked nucleic
... The next aim was to analyse in more detail the impact of the introduction of LNA and UNA at different positions in the siRNA on its antiviral activity. To this end, a set of sense and antisense strands with three nucleotide overhangs and UNA as well as LNA modifications at different positions was des ...
... The next aim was to analyse in more detail the impact of the introduction of LNA and UNA at different positions in the siRNA on its antiviral activity. To this end, a set of sense and antisense strands with three nucleotide overhangs and UNA as well as LNA modifications at different positions was des ...
peptide guideline-Application notes.ai
... Do not store excessive peptides in solution because the shelf life of peptides in solution is very limited, much shorter than lyophilized peptides. This is especially true for peptides whose sequences contain cysteine, methionine, tryptophan, asparagine, glutamine, or N-terminal glutamic acid. It is ...
... Do not store excessive peptides in solution because the shelf life of peptides in solution is very limited, much shorter than lyophilized peptides. This is especially true for peptides whose sequences contain cysteine, methionine, tryptophan, asparagine, glutamine, or N-terminal glutamic acid. It is ...
Isolated Spinach Ribulose-1,5-Bisphosphate
... transgenic tobacco plants,” Proc. Nat. Acad. Sci. USA 89:8394—8398 (1992)). These studies have been inconclu ...
... transgenic tobacco plants,” Proc. Nat. Acad. Sci. USA 89:8394—8398 (1992)). These studies have been inconclu ...
Seminario Glúcidos 3 y lípidos 1. Comente los mecanismos de
... of mitochondria which had been sedimented once in 10 volumes of 0.88 M sucrose as described above, and subjecting the suspension at this point to two or three preliminary sedimentations at low speed (2000 X g), each of 5 minutes duration. The main bulk of the mitochondria, now freed of red blood cel ...
... of mitochondria which had been sedimented once in 10 volumes of 0.88 M sucrose as described above, and subjecting the suspension at this point to two or three preliminary sedimentations at low speed (2000 X g), each of 5 minutes duration. The main bulk of the mitochondria, now freed of red blood cel ...
Introduction to Carbohydrates
... Congenital deficiencies in one of the components of the carnitine palmitoyltransferase system, in renal tubular reabsorption of carnitine, or in carnitine uptake by cells cause primary carnitine deficiency. Genetic CPT-I deficiency affects the liver, where an inability to use LCFA for fuel great ...
... Congenital deficiencies in one of the components of the carnitine palmitoyltransferase system, in renal tubular reabsorption of carnitine, or in carnitine uptake by cells cause primary carnitine deficiency. Genetic CPT-I deficiency affects the liver, where an inability to use LCFA for fuel great ...
Does premature aging of the mtDNA mutator mouse prove that
... of conflicting estimates of mutant fractions in wild-type mice). This conclusion, however, would probably be incorrect, in part because the types and distribution of mtDNA mutations in aging humans may be very different from those in mutator mice, which may allow them to play a role in aging despite ...
... of conflicting estimates of mutant fractions in wild-type mice). This conclusion, however, would probably be incorrect, in part because the types and distribution of mtDNA mutations in aging humans may be very different from those in mutator mice, which may allow them to play a role in aging despite ...
supplementary methods
... BGT147 has a hybrid intron 2 that contains the 5' A-globin region, Oct-1 site and 3' globin region created in several steps. First, the 5' A intron 2 was PCR amplified using B54 (S) and B54 Dra (AS) primers (Table S1) using BGT54 DNA as template. B54 Dra (AS) primer was used to create a DraI sit ...
... BGT147 has a hybrid intron 2 that contains the 5' A-globin region, Oct-1 site and 3' globin region created in several steps. First, the 5' A intron 2 was PCR amplified using B54 (S) and B54 Dra (AS) primers (Table S1) using BGT54 DNA as template. B54 Dra (AS) primer was used to create a DraI sit ...
NSPKU
... In PKU, the enzyme phenylalanine hydroxylase is not made by the liver or the enzyme does not work properly. This means that Phe is not converted to the amino acid Tyrosine and builds up in the blood. This is the reason a special diet low in protein / Phe needs to be followed. Low levels of Tyrosine ...
... In PKU, the enzyme phenylalanine hydroxylase is not made by the liver or the enzyme does not work properly. This means that Phe is not converted to the amino acid Tyrosine and builds up in the blood. This is the reason a special diet low in protein / Phe needs to be followed. Low levels of Tyrosine ...
Redacted for Privacy
... streak which can occur in childhood or adolescence. These early deposits of lipid contain free cholesterol, cholesterol esters, and triglycerides. Foam cells, which are lipid-laden macrophages, are also observed in this early lesion and are characteristic ...
... streak which can occur in childhood or adolescence. These early deposits of lipid contain free cholesterol, cholesterol esters, and triglycerides. Foam cells, which are lipid-laden macrophages, are also observed in this early lesion and are characteristic ...
Planta - University of Regina
... cDNAs prepared from total mRNAs as template yielded four approx. 600-bp core sequences. Full-length cDNA sequences including 5¢- and 3¢-untranslated regions of the four clones (PnJ, PnP, PnI and PnE) were obtained by employing the RACE method with primers derived from these core sequences. When a se ...
... cDNAs prepared from total mRNAs as template yielded four approx. 600-bp core sequences. Full-length cDNA sequences including 5¢- and 3¢-untranslated regions of the four clones (PnJ, PnP, PnI and PnE) were obtained by employing the RACE method with primers derived from these core sequences. When a se ...
The Art of Multiple Sequence Alignment in R
... idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for sequence alignment in DECIPHER is AlignProfiles, which takes in two aligned sets of DNA, RNA, or amino acid ( ...
... idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for sequence alignment in DECIPHER is AlignProfiles, which takes in two aligned sets of DNA, RNA, or amino acid ( ...
OLSON LAB PROTOCOL: Working with RNA
... designed to be ephemeral and are thus inherently highly unstable in comparison to DNA. Thinking in terms of gene expression While working with RNA poses technical challenges beyond those of working with DNA, it also requires a different way of thinking. Whereas a genomic DNA (gDNA) sample derived fr ...
... designed to be ephemeral and are thus inherently highly unstable in comparison to DNA. Thinking in terms of gene expression While working with RNA poses technical challenges beyond those of working with DNA, it also requires a different way of thinking. Whereas a genomic DNA (gDNA) sample derived fr ...
Metabolic acidosis
... • If not, then serum Na may be spuriously low OR osmolytes other then Na, glc or urea have accumulated. • The osmolar gap is a reliable and helpful tool when screening for toxin-associated high AG acidosis • Must correct for ETOH if present. ...
... • If not, then serum Na may be spuriously low OR osmolytes other then Na, glc or urea have accumulated. • The osmolar gap is a reliable and helpful tool when screening for toxin-associated high AG acidosis • Must correct for ETOH if present. ...
draft safety assessment report - Food Standards Australia New
... nutritional adequacy of the food, including whether there had been any unintended changes. No potential public health and safety concerns were identified in the assessment of food derived from cotton line COT102. Therefore, on the basis of all the available evidence, including detailed studies provi ...
... nutritional adequacy of the food, including whether there had been any unintended changes. No potential public health and safety concerns were identified in the assessment of food derived from cotton line COT102. Therefore, on the basis of all the available evidence, including detailed studies provi ...
Molecular characterization of MHC class II in a nonmodel anuran
... overrated (Acevedo-Whitehouse and Cunningham 2006), associations between certain MHC alleles and disease resistance or susceptibility have been found in a number of species (Sommer 2005). Overall, this marker has been found often to be more informative than neutral markers to judge the evolutionary ...
... overrated (Acevedo-Whitehouse and Cunningham 2006), associations between certain MHC alleles and disease resistance or susceptibility have been found in a number of species (Sommer 2005). Overall, this marker has been found often to be more informative than neutral markers to judge the evolutionary ...
Document
... To understand the complete biological role of p53 protein and its mutant phenotype we need to study the gene at multiple levels: Genetics- mutant gene- mutant phenotype Now what? Genetics will relate specific mutation to specific phenotype It usually provides No Information about how the protein gen ...
... To understand the complete biological role of p53 protein and its mutant phenotype we need to study the gene at multiple levels: Genetics- mutant gene- mutant phenotype Now what? Genetics will relate specific mutation to specific phenotype It usually provides No Information about how the protein gen ...
Chapter 2 - University of the Free State
... •At higher salt concentrations, weak interactions between the disordered water molecules are further disrupted, i.e., the molecules become even less ordered •transfer of a water molecule from the ordered environment next to the hydrophobic droplet to the disordered state, will have a larger ∆S compo ...
... •At higher salt concentrations, weak interactions between the disordered water molecules are further disrupted, i.e., the molecules become even less ordered •transfer of a water molecule from the ordered environment next to the hydrophobic droplet to the disordered state, will have a larger ∆S compo ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... highly conserved regions and divergent regions. Evolutionary distances are determined from the nucleotide or amino acid differences within the divergent regions, while primers are created from the highly conserved regions flanking a divergent region. The ideal primer for a particular protein or gene ...
... highly conserved regions and divergent regions. Evolutionary distances are determined from the nucleotide or amino acid differences within the divergent regions, while primers are created from the highly conserved regions flanking a divergent region. The ideal primer for a particular protein or gene ...
Chapter 1 Introduction to Evolutionary Computation and Genetic
... Solving many computational problems can be formulated as a problem of trying to find a string of symbols that lead to a solution. For instance, a well-known problem in computer science is called the travelling salesman problem (TSP). In this problem one wishes to find the shortest route that will vi ...
... Solving many computational problems can be formulated as a problem of trying to find a string of symbols that lead to a solution. For instance, a well-known problem in computer science is called the travelling salesman problem (TSP). In this problem one wishes to find the shortest route that will vi ...
1958 Shorland: RECENT WORK ON ANIMAL FATS
... to the presence of a rumen in the sheep with its populations of active Those specializing in ruminant metabolism, micro-organisms. ;as highly significant for the who rightly regard the rumen breakdoivn of dietary cellulose and protein, have overlooked its effect on dietary fat or even emphasized tha ...
... to the presence of a rumen in the sheep with its populations of active Those specializing in ruminant metabolism, micro-organisms. ;as highly significant for the who rightly regard the rumen breakdoivn of dietary cellulose and protein, have overlooked its effect on dietary fat or even emphasized tha ...
Genetic code
The genetic code is the set of rules by which information encoded within genetic material (DNA or mRNA sequences) is translated into proteins by living cells. Biological decoding is accomplished by the ribosome, which links amino acids in an order specified by mRNA, using transfer RNA (tRNA) molecules to carry amino acids and to read the mRNA three nucleotides at a time. The genetic code is highly similar among all organisms and can be expressed in a simple table with 64 entries.The code defines how sequences of these nucleotide triplets, called codons, specify which amino acid will be added next during protein synthesis. With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. Because the vast majority of genes are encoded with exactly the same code (see the RNA codon table), this particular code is often referred to as the canonical or standard genetic code, or simply the genetic code, though in fact some variant codes have evolved. For example, protein synthesis in human mitochondria relies on a genetic code that differs from the standard genetic code.While the genetic code determines the protein sequence for a given coding region, other genomic regions can influence when and where these proteins are produced.