NZY M-MuLV Reverse Transcriptase
... RNase H activity, enabling improved synthesis of full-length cDNA, even for long mRNA, using random priming. Thus, the enzyme gives high yields of first-strand cDNA up to 7 kb. NZY M-MuLV Reverse Transcriptase can be used in firststrand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and ...
... RNase H activity, enabling improved synthesis of full-length cDNA, even for long mRNA, using random priming. Thus, the enzyme gives high yields of first-strand cDNA up to 7 kb. NZY M-MuLV Reverse Transcriptase can be used in firststrand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and ...
Residue 2 residue statistics
... any non-hydrogen atoms within each residue are within 3.5 Å of each other. Statistics are ONLY collected for interactions of residues within the same protein of the viral capsid. All viruses used have icosahedral symmetry. A protein was used to collect statistics if it was a ...
... any non-hydrogen atoms within each residue are within 3.5 Å of each other. Statistics are ONLY collected for interactions of residues within the same protein of the viral capsid. All viruses used have icosahedral symmetry. A protein was used to collect statistics if it was a ...
Protocol for QuickExtract™ RNA Extraction Kit
... 1. For cDNA synthesis, 1-10 μl of extracted RNA can be used directly with any reverse transcriptase and a standard 20-μl protocol. Up to 50% of the reaction volume can be extracted RNA. 2. For standard and fast end-point PCR cycling profiles, use 1-5 μl of cDNA. 3. When using extracts in real-tim ...
... 1. For cDNA synthesis, 1-10 μl of extracted RNA can be used directly with any reverse transcriptase and a standard 20-μl protocol. Up to 50% of the reaction volume can be extracted RNA. 2. For standard and fast end-point PCR cycling profiles, use 1-5 μl of cDNA. 3. When using extracts in real-tim ...
S11. Computational Molecular Modeling- Week 5. 3
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
Chapter 5.9 THE USE OF D-AMINO ACIDS IN PEPTIDE DESIGN
... In the synthesis of analogs of biologically active peptides, it is often desirable to introduce D-amino acids into host sequences composed of L-amino acids. The site of D-amino acid insertion becomes resistant to proteolytic cleavage, thereby resulting in greater in vivo stability of such analogs [3 ...
... In the synthesis of analogs of biologically active peptides, it is often desirable to introduce D-amino acids into host sequences composed of L-amino acids. The site of D-amino acid insertion becomes resistant to proteolytic cleavage, thereby resulting in greater in vivo stability of such analogs [3 ...
Developmental Analysis of a Putative ATP/ADP Carrier Protein
... cotyledons, and in the isolated peroxisomes, respectively, indicating that the antibody was monospecific. We concluded that the antibody cross-reacted with not only AtPMP38 but also PmPMP38, although it was indicated that the molecular mass of PmPMP38 was slightly higher than that of AtPMP38. Subcel ...
... cotyledons, and in the isolated peroxisomes, respectively, indicating that the antibody was monospecific. We concluded that the antibody cross-reacted with not only AtPMP38 but also PmPMP38, although it was indicated that the molecular mass of PmPMP38 was slightly higher than that of AtPMP38. Subcel ...
Chapter 17: An Introduction to Organic Chemistry, Biochemistry, and
... at noon to drive four blocks to get some lunch. The gasoline that fuels the car is composed of many different organic compounds, including some belonging to the category of organic compounds called alkanes and a fuel additive called methyl t-butyl ether (MTBE). When they get to the restaurant, Stuar ...
... at noon to drive four blocks to get some lunch. The gasoline that fuels the car is composed of many different organic compounds, including some belonging to the category of organic compounds called alkanes and a fuel additive called methyl t-butyl ether (MTBE). When they get to the restaurant, Stuar ...
Induced point mutations in the phytoene synthase 1 gene cause
... samples from all fruit were analysed for carotenoid content using HPLC, as described in Materials and Methods (Fig. 4). Four metabolites were measured: phytoene, the direct PSY product; lycopene, the main compound responsible for the red colour of tomato fruit; b-carotene; and lutein. In the Psy1 no ...
... samples from all fruit were analysed for carotenoid content using HPLC, as described in Materials and Methods (Fig. 4). Four metabolites were measured: phytoene, the direct PSY product; lycopene, the main compound responsible for the red colour of tomato fruit; b-carotene; and lutein. In the Psy1 no ...
Regulation of biosynthesis and transport of aromatic amino acids in
... Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram-positive bacteria. A new candidate transcription regulation signal of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococc ...
... Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram-positive bacteria. A new candidate transcription regulation signal of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococc ...
Biochemistry of Cells
... Many proteins act as biological catalysts or enzymes Thousands of different enzymes exist in the body Enzymes control the rate of chemical reactions by weakening bonds, thus lowering the amount of activation energy needed for the reaction Copyright Cmassengale ...
... Many proteins act as biological catalysts or enzymes Thousands of different enzymes exist in the body Enzymes control the rate of chemical reactions by weakening bonds, thus lowering the amount of activation energy needed for the reaction Copyright Cmassengale ...
1 Epistasis Underlying a Fitness Trait within a Natural
... populations within a 200 m radius of each other (Bradshaw et al. 2003): "Stream side," from along the stream itself; "Backwater," from a backwater of the stream about 100 m north of the first collection site; "Sandy bog," a sandy bog about 300 m to the west of the stream and separated from it by dry ...
... populations within a 200 m radius of each other (Bradshaw et al. 2003): "Stream side," from along the stream itself; "Backwater," from a backwater of the stream about 100 m north of the first collection site; "Sandy bog," a sandy bog about 300 m to the west of the stream and separated from it by dry ...
high-performance gene expression
... quadruplex reactions (red line). The results illustrate that SensiFAST Probe No-ROX has high sensitivity and excellent reproducibility for both singleplex and multiplex reactions (Fig. 7A) and no reduction of efficiency (Fig. 7B) that is often associated with multiplexing. There is no change in Ct v ...
... quadruplex reactions (red line). The results illustrate that SensiFAST Probe No-ROX has high sensitivity and excellent reproducibility for both singleplex and multiplex reactions (Fig. 7A) and no reduction of efficiency (Fig. 7B) that is often associated with multiplexing. There is no change in Ct v ...
to Sample Chapter
... v. Awarded Nobel prize. i. Took interest in both proteins and nucleic acid at Cambridge in UK ii. Shared Nobel prize for medicine and physiology for cracking the genetic code. i. Most noted for one of two co-discoveries of the structure of DNA molecule. ii. Awarded Nobel prize i. Two times awarded N ...
... v. Awarded Nobel prize. i. Took interest in both proteins and nucleic acid at Cambridge in UK ii. Shared Nobel prize for medicine and physiology for cracking the genetic code. i. Most noted for one of two co-discoveries of the structure of DNA molecule. ii. Awarded Nobel prize i. Two times awarded N ...
Biochemistry of Cells - Campbell County Schools
... Many proteins act as biological catalysts or enzymes Thousands of different enzymes exist in the body Enzymes control the rate of chemical reactions by weakening bonds, thus lowering the amount of activation energy needed for the reaction Copyright Cmassengale ...
... Many proteins act as biological catalysts or enzymes Thousands of different enzymes exist in the body Enzymes control the rate of chemical reactions by weakening bonds, thus lowering the amount of activation energy needed for the reaction Copyright Cmassengale ...
Functional analysis of an interspecies chimera of acyl carrier
... condensation reaction between acetyl-CoA and malonyl-ACP. This is the only condensation step in which CoA is used to donate substrates. In all other steps only fatty acids donated by ACP are used. Therefore ACPs are central proteins in fatty acid biosynthesis as they carry the malonyl elongation uni ...
... condensation reaction between acetyl-CoA and malonyl-ACP. This is the only condensation step in which CoA is used to donate substrates. In all other steps only fatty acids donated by ACP are used. Therefore ACPs are central proteins in fatty acid biosynthesis as they carry the malonyl elongation uni ...
Hazardous Substances and New Organisms (Low
... developments using micro-organisms as a host or vector that are normally able to cause disease in humans, animals, plants, or fungi and that use defective vector/helper virus combinations with the potential to regenerate a non-defective recombinant virus other than those that satisfy the requirement ...
... developments using micro-organisms as a host or vector that are normally able to cause disease in humans, animals, plants, or fungi and that use defective vector/helper virus combinations with the potential to regenerate a non-defective recombinant virus other than those that satisfy the requirement ...
www.LessonPlansInc.com
... Standards: CA Biology 5c Students know how mutations in the DNA sequence of a gene may or may not affect the expression of the gene or the sequence of amino acids in an encoded protein. 5e Students know proteins can differ from one another in the number and sequence of amino acids. 7a Students know ...
... Standards: CA Biology 5c Students know how mutations in the DNA sequence of a gene may or may not affect the expression of the gene or the sequence of amino acids in an encoded protein. 5e Students know proteins can differ from one another in the number and sequence of amino acids. 7a Students know ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... including a gap of 63 amino acids and an additional 16 amino acids at the carboxyl terminus, which may play some role in modifying the U-DNA interaction, such that a promoter sequence unique to C. trachomatis is recognized. Monoclonal antibodies specific for E. coli U” were used to probe for homolog ...
... including a gap of 63 amino acids and an additional 16 amino acids at the carboxyl terminus, which may play some role in modifying the U-DNA interaction, such that a promoter sequence unique to C. trachomatis is recognized. Monoclonal antibodies specific for E. coli U” were used to probe for homolog ...
doc
... B. Probability of obtaining a match of that quality due to chance. C. Number of standard deviations a match is above mean, generated by randomizing sequences. D. The measure derived from primary sequence similarity divided by the length of the match. E. A measure of how similar two secondary structu ...
... B. Probability of obtaining a match of that quality due to chance. C. Number of standard deviations a match is above mean, generated by randomizing sequences. D. The measure derived from primary sequence similarity divided by the length of the match. E. A measure of how similar two secondary structu ...
Document
... 1 Substrates bind 2 Internal at active site. rearrangements Enzyme changes leading to shape to hold catalysis occur. substrates in proper position. ...
... 1 Substrates bind 2 Internal at active site. rearrangements Enzyme changes leading to shape to hold catalysis occur. substrates in proper position. ...
rationale_for_searching_seq_db - Cal State LA
... of “hits” one can "expect" to see just by chance when searching a database of a particular size. It decreases exponentially as the Similarity Score (S) increases (inverse relationship). The higher the Similarity Score, the lower the E value. Essentially, the E value describes the random background n ...
... of “hits” one can "expect" to see just by chance when searching a database of a particular size. It decreases exponentially as the Similarity Score (S) increases (inverse relationship). The higher the Similarity Score, the lower the E value. Essentially, the E value describes the random background n ...
Lysine-Restricted Diet as Adjunct Therapy for Pyridoxine
... metabolic manipulation. The goal of dietary treatment is to restrict lysine as a precursor of potentially neurotoxic intermediates generated by disturbed lysine degradation, whilst avoiding lysine deficiency and maintaining sufficient intake of essential nutrients and energy substrates. Although a d ...
... metabolic manipulation. The goal of dietary treatment is to restrict lysine as a precursor of potentially neurotoxic intermediates generated by disturbed lysine degradation, whilst avoiding lysine deficiency and maintaining sufficient intake of essential nutrients and energy substrates. Although a d ...
TARGET: a new method for predicting protein subcellular
... Therefore, experimental annotation of protein subcellular localization is not able to keep up with the large number of sequences that continue to emerge from the genome sequencing projects. To bridge this gap, there is a need to develop faster, accurate and genomescale computational methods for pred ...
... Therefore, experimental annotation of protein subcellular localization is not able to keep up with the large number of sequences that continue to emerge from the genome sequencing projects. To bridge this gap, there is a need to develop faster, accurate and genomescale computational methods for pred ...
Which Protein is Best?
... to the faster digestion and absorption kinetics of whey, which results in a greater increase in postprandial plasma amino acid availability and thereby further stimulates muscle protein synthesis,” report the researchers. Although previous research shows that whey protein supports greater protein re ...
... to the faster digestion and absorption kinetics of whey, which results in a greater increase in postprandial plasma amino acid availability and thereby further stimulates muscle protein synthesis,” report the researchers. Although previous research shows that whey protein supports greater protein re ...
Feeding Strategies to Lower Nitrogen and
... feed ingredients used and formulate diets based on an available nutrient basis. Because of variability composition, routine laboratory feed analyses and a ration formulation program are necessary to adjust diets and maintain minimum nutrient excretions. Careful selection of feed ingredients also is ...
... feed ingredients used and formulate diets based on an available nutrient basis. Because of variability composition, routine laboratory feed analyses and a ration formulation program are necessary to adjust diets and maintain minimum nutrient excretions. Careful selection of feed ingredients also is ...
Genetic code
The genetic code is the set of rules by which information encoded within genetic material (DNA or mRNA sequences) is translated into proteins by living cells. Biological decoding is accomplished by the ribosome, which links amino acids in an order specified by mRNA, using transfer RNA (tRNA) molecules to carry amino acids and to read the mRNA three nucleotides at a time. The genetic code is highly similar among all organisms and can be expressed in a simple table with 64 entries.The code defines how sequences of these nucleotide triplets, called codons, specify which amino acid will be added next during protein synthesis. With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. Because the vast majority of genes are encoded with exactly the same code (see the RNA codon table), this particular code is often referred to as the canonical or standard genetic code, or simply the genetic code, though in fact some variant codes have evolved. For example, protein synthesis in human mitochondria relies on a genetic code that differs from the standard genetic code.While the genetic code determines the protein sequence for a given coding region, other genomic regions can influence when and where these proteins are produced.