Glossary
... AIDS, acquired immunodeficiency syndrome - An incurable (at the time of this writing) disease that results from infection by the human immunodeficiency (HIV) virus. AIDS symptoms build up as the concentration of HIV viruses in the body increases, usually over a period of years. Eventually, AIDS vict ...
... AIDS, acquired immunodeficiency syndrome - An incurable (at the time of this writing) disease that results from infection by the human immunodeficiency (HIV) virus. AIDS symptoms build up as the concentration of HIV viruses in the body increases, usually over a period of years. Eventually, AIDS vict ...
Transient Overexpression of Recombinant Proteins in Mammalian
... Flow cytometry was used to quantify the expression levels of AcGFP1 in adherent and suspension cells. Cells transfected with pHEK293 Ultra Expression Vector I (conditions 2 and 7) and pHEK293 Ultra Expression Vector II (conditions 3, 4, 8, and 9) displayed fluorescence intensities 5–11 times higher ...
... Flow cytometry was used to quantify the expression levels of AcGFP1 in adherent and suspension cells. Cells transfected with pHEK293 Ultra Expression Vector I (conditions 2 and 7) and pHEK293 Ultra Expression Vector II (conditions 3, 4, 8, and 9) displayed fluorescence intensities 5–11 times higher ...
7. Biology Glossary
... Alga, Algal, Algae: A group of plant-like organisms that include the one-celled organisms that form phytoplankton as well as the seaweeds. Although algae photosynthesise and their cells are surrounded by a cell wall, they are not plants. They belong to a group of organisms called the Protoctista. A ...
... Alga, Algal, Algae: A group of plant-like organisms that include the one-celled organisms that form phytoplankton as well as the seaweeds. Although algae photosynthesise and their cells are surrounded by a cell wall, they are not plants. They belong to a group of organisms called the Protoctista. A ...
UNIT-I Chapter-2 Biological Classification
... Cell walls are embedded with silica. 2. How are Viroids different from Viruses? A: Viroids contain only nucleic acid and no protein coat. Nucleic acid is RNA. Viruses contain both nucleic acid and protein. Nucleic acid is DNA or RNA. 3. What do the terms phycobiont and mycobiont signify? A: Lichens ...
... Cell walls are embedded with silica. 2. How are Viroids different from Viruses? A: Viroids contain only nucleic acid and no protein coat. Nucleic acid is RNA. Viruses contain both nucleic acid and protein. Nucleic acid is DNA or RNA. 3. What do the terms phycobiont and mycobiont signify? A: Lichens ...
Transporters as drug carriers and targets in the GIT
... gradient set up across the membrane using another ion. Because this secondary diffusion gradient initially established using an ion pump, as in primary active transport, the energy is ultimately derived from the same source-ATP hydrolysis. ...
... gradient set up across the membrane using another ion. Because this secondary diffusion gradient initially established using an ion pump, as in primary active transport, the energy is ultimately derived from the same source-ATP hydrolysis. ...
Caspase Colorimetric Protease Assay Sampler Kit (Caspases-2,
... determination of caspase proteolytic activity in lysates of mammalian cells. This test is designed for research use only. It is not to be used in diagnostic procedures. PRINCIPLE OF THE METHOD Apoptosis, or programmed cell death, is the outcome of a programmed intracellular cascade of genetically de ...
... determination of caspase proteolytic activity in lysates of mammalian cells. This test is designed for research use only. It is not to be used in diagnostic procedures. PRINCIPLE OF THE METHOD Apoptosis, or programmed cell death, is the outcome of a programmed intracellular cascade of genetically de ...
A Cell Biology Laboratory Exercise to Study Sub
... The fast changing scenario of undergraduate education puts emphasis on introducing students to hands-on techniques as part of their laboratory courses. In order to cater to large numbers of students and the time constraints involved with undergraduate level laboratory courses, there is a need for de ...
... The fast changing scenario of undergraduate education puts emphasis on introducing students to hands-on techniques as part of their laboratory courses. In order to cater to large numbers of students and the time constraints involved with undergraduate level laboratory courses, there is a need for de ...
L-Tyrosine - Douglas Labs
... Proteins and amino acids also serve as a source of energy, providing about 4 calories per gram. Aside from these general functions, individual amino acids also have specific functions in many aspects of human physiology and biochemistry. Tyrosine is a precursor of important neurotransmitters in the ...
... Proteins and amino acids also serve as a source of energy, providing about 4 calories per gram. Aside from these general functions, individual amino acids also have specific functions in many aspects of human physiology and biochemistry. Tyrosine is a precursor of important neurotransmitters in the ...
IMBB researchers uncover a novel mechanism
... eukaryotic cells that also play pivotal roles in fundamental cellular processes. Alterations in mitochondrial number, morphology, and function heavily impact cellular metabolism, and critically influence organismal physiology, health and lifespan. Mitochondrial dysfunction is a major hallmark of age ...
... eukaryotic cells that also play pivotal roles in fundamental cellular processes. Alterations in mitochondrial number, morphology, and function heavily impact cellular metabolism, and critically influence organismal physiology, health and lifespan. Mitochondrial dysfunction is a major hallmark of age ...
Active transport of antibiotics across the outer membrane of gram
... proteins; this binding greatly increases the efficiency of iron uptake. E. coli K-12 expresses 7 such iron siderophore transport systems. The siderophores can be the products of bacteria or fungi. The many transport systems for just a single metal reflects the variety of siderophores in the surround ...
... proteins; this binding greatly increases the efficiency of iron uptake. E. coli K-12 expresses 7 such iron siderophore transport systems. The siderophores can be the products of bacteria or fungi. The many transport systems for just a single metal reflects the variety of siderophores in the surround ...
Biochemistry - Science with Mr. Enns
... Monomer vs. Polymer Take a pearl necklace, for example: ...
... Monomer vs. Polymer Take a pearl necklace, for example: ...
2ndDisinfection
... Surface-active agents • Amphiphilic compounds • Anionic, cationic, nonionic, and amphoterics • Cationic surfactants: Quaternary ammonium compounds – Basic structure: • One nitrogen atom • Four carbon atoms covalently linked to the nitrogen atom • An anion eletrostatically linked to the nitrogen ato ...
... Surface-active agents • Amphiphilic compounds • Anionic, cationic, nonionic, and amphoterics • Cationic surfactants: Quaternary ammonium compounds – Basic structure: • One nitrogen atom • Four carbon atoms covalently linked to the nitrogen atom • An anion eletrostatically linked to the nitrogen ato ...
Transcription and Translation ppt
... essential proteins such as antibodies and hormones. Proteins drive cellular processes such as metabolism; determining physical characteristics and producing genetic disorders by their absence or presence in an altered form. ...
... essential proteins such as antibodies and hormones. Proteins drive cellular processes such as metabolism; determining physical characteristics and producing genetic disorders by their absence or presence in an altered form. ...
Prolyl Isomerases –Old Proteins as New Therapeutic Targets
... The early pioneering work by Fischer concerned the enzymology of protein folding, a biochemical event unlikely to show up in the massive “target identification and validation” efforts that have taken place in pharmaceutical and biotechnology companies over the last 20 years. Moreover, a publication ...
... The early pioneering work by Fischer concerned the enzymology of protein folding, a biochemical event unlikely to show up in the massive “target identification and validation” efforts that have taken place in pharmaceutical and biotechnology companies over the last 20 years. Moreover, a publication ...
Chapter 7
... • Small molecules and water enter or leave the cell through the lipid bilayer or by transport proteins ...
... • Small molecules and water enter or leave the cell through the lipid bilayer or by transport proteins ...
PPT - Altogen Biosystems
... Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells) Altogen Biosystems offers the HeLa Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HeLa Transfection Reagent is an liposome formulation reagent, and it has been developed to provide high t ...
... Products > HeLa Transfection Reagent (Cervix Adenocarcinoma Cells) Altogen Biosystems offers the HeLa Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HeLa Transfection Reagent is an liposome formulation reagent, and it has been developed to provide high t ...
Tendon Gene Therapy Modulates the Local Repair Environment in
... the following was performed: RTF cells were seeded onto a bioabsorbable polymer scaffold composed of polyglycolic acid and cultured for five days to assemble a tissue engineered tendon construct. This technique was then repeated to assemble constructs containing the PDGF- gene transduced RTFs. The t ...
... the following was performed: RTF cells were seeded onto a bioabsorbable polymer scaffold composed of polyglycolic acid and cultured for five days to assemble a tissue engineered tendon construct. This technique was then repeated to assemble constructs containing the PDGF- gene transduced RTFs. The t ...
Antibodies for Unfolded Protein Response
... PERK, predicted molecular weight 125.2 kDa, is a transmembrane protein kinase belonging to the PEK family of proteins, and is best known for its role in insulin processing. During ER stress responses and activation of the UPR, PERK functions to inhibit translation of new proteins. Specifically, ER s ...
... PERK, predicted molecular weight 125.2 kDa, is a transmembrane protein kinase belonging to the PEK family of proteins, and is best known for its role in insulin processing. During ER stress responses and activation of the UPR, PERK functions to inhibit translation of new proteins. Specifically, ER s ...
SDS-PAGE and Western blotting
... Fig.1Before SDS: Protein (pink line) incubated with the denaturing detergent SDS showing negative and positive charges due to the charged R‐groups in the protein. The large H's represent hydrophobic domains where nonpolar R‐groups have collected in an attempt to get away from the polar water th ...
... Fig.1Before SDS: Protein (pink line) incubated with the denaturing detergent SDS showing negative and positive charges due to the charged R‐groups in the protein. The large H's represent hydrophobic domains where nonpolar R‐groups have collected in an attempt to get away from the polar water th ...
chapter 13 section 2 notes
... peptide bond between the first and second amino acids— methionine and phenylalanine. At the same time, the bond holding the first tRNA molecule to its amino acid is broken. ...
... peptide bond between the first and second amino acids— methionine and phenylalanine. At the same time, the bond holding the first tRNA molecule to its amino acid is broken. ...
Nanomaterials: DNA brings quantum dots to order
... change in cellular compartments. An obvious future direction is to explore quantum dot complexes that can respond to other environmental triggers, such as changes in ion concentrations or the presence of biomarkers. The strategy developed by Kelley and co-workers could also be readily used to create ...
... change in cellular compartments. An obvious future direction is to explore quantum dot complexes that can respond to other environmental triggers, such as changes in ion concentrations or the presence of biomarkers. The strategy developed by Kelley and co-workers could also be readily used to create ...
Document
... Research stemming from the study of SAMs (e.g., alkylthiols and other biomembrane mimics on gold) led to the discovery of “stamping” (Figure 7.4) (Kumar and Whitesides 1993). This method, in which an elastomeric stamp is used for rapid pattern transfer, has now been driven to < 50 nanometer scales a ...
... Research stemming from the study of SAMs (e.g., alkylthiols and other biomembrane mimics on gold) led to the discovery of “stamping” (Figure 7.4) (Kumar and Whitesides 1993). This method, in which an elastomeric stamp is used for rapid pattern transfer, has now been driven to < 50 nanometer scales a ...
chapter 17 from gene to protein
... Once it reaches the cytoplasm, each tRNA is used repeatedly, picking up its designated amino acid in the cytosol, depositing the amino acid at the ribosome, and returning to the cytosol to pick up another copy of that amino acid. ...
... Once it reaches the cytoplasm, each tRNA is used repeatedly, picking up its designated amino acid in the cytosol, depositing the amino acid at the ribosome, and returning to the cytosol to pick up another copy of that amino acid. ...
Protein Synthesis - Austin Community College
... Once it reaches the cytoplasm, each tRNA is used repeatedly, picking up its designated amino acid in the cytosol, depositing the amino acid at the ribosome, and returning to the cytosol to pick up another copy of that amino acid. ...
... Once it reaches the cytoplasm, each tRNA is used repeatedly, picking up its designated amino acid in the cytosol, depositing the amino acid at the ribosome, and returning to the cytosol to pick up another copy of that amino acid. ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.