1 laboratory 9 construction of a fusion protein
... keep the cells growing for many generations. In transient transfections, the DNA is maintained extrachromasomally (meaning as a separate piece of DNA in the nucleus). Since the DNA does not replicate or attach to the mitotic spindle efficiently, it will be lost as the cells replicate and divide, typ ...
... keep the cells growing for many generations. In transient transfections, the DNA is maintained extrachromasomally (meaning as a separate piece of DNA in the nucleus). Since the DNA does not replicate or attach to the mitotic spindle efficiently, it will be lost as the cells replicate and divide, typ ...
Product Datasheet TERT Antibody NB110
... Telomerase reverse transcriptase antibody used as stem/progenitor cell marker in ICC-IF application on human corneal endothelial cells grown with serial explant culture technique and explant culture wounding model. Bandhakavi S, Kim YM, Ro SH et al. Quantitative nuclear proteomics identifies mTOR re ...
... Telomerase reverse transcriptase antibody used as stem/progenitor cell marker in ICC-IF application on human corneal endothelial cells grown with serial explant culture technique and explant culture wounding model. Bandhakavi S, Kim YM, Ro SH et al. Quantitative nuclear proteomics identifies mTOR re ...
“Synthesis, characterization and biomedical applications of microbial polymalic and polyglutamic acids derivatives.”
... the manufacture of nanoparticles with a diameter in the 200 nm range and a fairly narrow size distribution. These nanoparticles are readily hydrodegradable following almost linear profiles and taking place within periods of time of 15 to 40 days. The degradation rate is depending of both the length ...
... the manufacture of nanoparticles with a diameter in the 200 nm range and a fairly narrow size distribution. These nanoparticles are readily hydrodegradable following almost linear profiles and taking place within periods of time of 15 to 40 days. The degradation rate is depending of both the length ...
CRACKING THE GENETIC CODE
... amino acids. The genes that encode proteins—that is, specify the type and linear order of their component amino acids—are located in DNA, a polymer made up of only four different nucleotides. The DNA code is transcribed into RNA, which is also composed of four nucleotides. Nirenberg’s studies were p ...
... amino acids. The genes that encode proteins—that is, specify the type and linear order of their component amino acids—are located in DNA, a polymer made up of only four different nucleotides. The DNA code is transcribed into RNA, which is also composed of four nucleotides. Nirenberg’s studies were p ...
Gene Section PRUNE (prune exopolyphosphatase) Atlas of Genetics and Cytogenetics
... Prune, a 62 kDa protein, belongs to the DHH family phosphoesterase proteins including RecJ DNA repair exonucleases, pyrophosphatases (PPASEs) and exopolyphospatases (PPX). The DHH super-family can be divided into two main groups on the basis of a Cterminal motif that is very well conserved within ea ...
... Prune, a 62 kDa protein, belongs to the DHH family phosphoesterase proteins including RecJ DNA repair exonucleases, pyrophosphatases (PPASEs) and exopolyphospatases (PPX). The DHH super-family can be divided into two main groups on the basis of a Cterminal motif that is very well conserved within ea ...
PPT - Altogen Biosystems
... Products > Beta-TC-6 Transfection Reagent (Pancreas Tumor, CRL-11506) Altogen Biosystems offers the Beta-TC-6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Beta-TC-6 Transfection Reagent is a two component lipidmediated formulation, and it has been deve ...
... Products > Beta-TC-6 Transfection Reagent (Pancreas Tumor, CRL-11506) Altogen Biosystems offers the Beta-TC-6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Beta-TC-6 Transfection Reagent is a two component lipidmediated formulation, and it has been deve ...
Micropreparation of tissue collagenase fragments of type I collagen
... molecular size, larger peptides being eluted later than the smaller ones. It was hypothesized that the separation reflects the number of domains capable of interaction with the bare silica capillary wall; owing to the large internal homogeneity of collagen polypeptide chains, larger peptides must ne ...
... molecular size, larger peptides being eluted later than the smaller ones. It was hypothesized that the separation reflects the number of domains capable of interaction with the bare silica capillary wall; owing to the large internal homogeneity of collagen polypeptide chains, larger peptides must ne ...
Assessment and evaluation of human muscle, nervous system and
... Mechanical muscle function in vivo Per Aagaard: Assessment and evaluation of mechanical muscle function in vivo - paradigms and applications from elite sports to patient rehabilitation Human tendon and aponeurosis function in vivo Peter Magnusson: Assessment and evaluation of muscle tendon and apone ...
... Mechanical muscle function in vivo Per Aagaard: Assessment and evaluation of mechanical muscle function in vivo - paradigms and applications from elite sports to patient rehabilitation Human tendon and aponeurosis function in vivo Peter Magnusson: Assessment and evaluation of muscle tendon and apone ...
Virology
... Viruses multiply only in living cells. I order viruses to replicate; viral proteins must be synthesized by the host cell protein synthesizing machinery. The unique feature of viral multiplication is that soon after interaction with the host cell, the infecting virion is disrupted, this phase of the ...
... Viruses multiply only in living cells. I order viruses to replicate; viral proteins must be synthesized by the host cell protein synthesizing machinery. The unique feature of viral multiplication is that soon after interaction with the host cell, the infecting virion is disrupted, this phase of the ...
Tissues. Epithelial tissue. Glands.
... Body tissues are grouped according to their cells and cell products into organs. These tissues exist and function in close association with one another. Epithelial tissue is present in the two major forms: as sheets of contiguous cells (epithelia) that cover body on its external surface and as gland ...
... Body tissues are grouped according to their cells and cell products into organs. These tissues exist and function in close association with one another. Epithelial tissue is present in the two major forms: as sheets of contiguous cells (epithelia) that cover body on its external surface and as gland ...
CONCISE REPORT Late Expression of M and N
... of the surface-labeled membrane lysates were made with monoclonal antibodies 8 I 59 or 8 161, followed by rabbit anti-mouse ...
... of the surface-labeled membrane lysates were made with monoclonal antibodies 8 I 59 or 8 161, followed by rabbit anti-mouse ...
PPT - Altogen Biosystems
... Products > Caco-2 Cells Transfection Reagent (Colon Adenocarcinoma Cell, HTB-37) Altogen Biosystems offers the Caco-2 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Caco-2 Transfection Reagent is a biodegradable polymer based transfection reagent that en ...
... Products > Caco-2 Cells Transfection Reagent (Colon Adenocarcinoma Cell, HTB-37) Altogen Biosystems offers the Caco-2 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Caco-2 Transfection Reagent is a biodegradable polymer based transfection reagent that en ...
Protein Folding using Fluorescence Spectroscopy
... Phenylalanine displays the shortest absorption and emission wavelengths. Phenylalanine displays a structured emission with a maximum near 282 nm. The emission of tyrosine in water occurs at 303 nm and is relatively insensitive to solvent polarity. The emission maximum of tryptophan in water occurs n ...
... Phenylalanine displays the shortest absorption and emission wavelengths. Phenylalanine displays a structured emission with a maximum near 282 nm. The emission of tyrosine in water occurs at 303 nm and is relatively insensitive to solvent polarity. The emission maximum of tryptophan in water occurs n ...
miRNA - apctp
... is identical to the one of al-1 mutants. • This phenomenon was termed quelling. ...
... is identical to the one of al-1 mutants. • This phenomenon was termed quelling. ...
Las proteínas funcionan uniéndose en forma específica a
... Complete con verbos en tiempo perfecto ( pasado, presente, futuro, continuo) ...
... Complete con verbos en tiempo perfecto ( pasado, presente, futuro, continuo) ...
The Play is the thing… - Biology Learning Center
... • Only methionine tRNA (it will ‘know itself’ once crowned by the synthetase that hands out met) can team with small ribosomal subunit & join with the ...
... • Only methionine tRNA (it will ‘know itself’ once crowned by the synthetase that hands out met) can team with small ribosomal subunit & join with the ...
apbio ch 17 study guide
... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
Time-of-Flight Mass Spectrometry
... selecting a particular peptide mass, amino acid sequence ions can be obtained for each individual peptide in the mixture. In collaboration with Mark Soloski at Johns Hopkins we have used the AXIMA-CFR to characterize an endogenous peptide GMKFDRGYI derived from heat shock protein. This study, carrie ...
... selecting a particular peptide mass, amino acid sequence ions can be obtained for each individual peptide in the mixture. In collaboration with Mark Soloski at Johns Hopkins we have used the AXIMA-CFR to characterize an endogenous peptide GMKFDRGYI derived from heat shock protein. This study, carrie ...
Amino Acids and Their Properties
... Amino acid matching works better as evolutionary distance increases (why?) We’d like to be able to assess relatedness of organisms that diverged long ago ...
... Amino acid matching works better as evolutionary distance increases (why?) We’d like to be able to assess relatedness of organisms that diverged long ago ...
Enrichment for submitotic cell populations using flow cytometry
... found to be most enriched (84%) in the mid-cyclin B1, high phosphorylated histone H3 fractions; metaphase cells were most enriched (28%) in the high cyclin B1, high phosphorylated histone H3 fraction. These findings are in agreement with data from previous studies using only cyclin B1 flow cytometry ...
... found to be most enriched (84%) in the mid-cyclin B1, high phosphorylated histone H3 fractions; metaphase cells were most enriched (28%) in the high cyclin B1, high phosphorylated histone H3 fraction. These findings are in agreement with data from previous studies using only cyclin B1 flow cytometry ...
PPT - Altogen Biosystems
... Products > Transfection Reagent for BALB/3T3 Cells (Embryo Fibroblast Cells, CCL-163) Altogen Biosystems offers the BALB/3T3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The BALB/3T3 Transfection Reagent is a nanoparticle-based liposome formulation reagent ...
... Products > Transfection Reagent for BALB/3T3 Cells (Embryo Fibroblast Cells, CCL-163) Altogen Biosystems offers the BALB/3T3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The BALB/3T3 Transfection Reagent is a nanoparticle-based liposome formulation reagent ...
Practical Guide: Selecting the Optimal Resins for Removal of DNA
... purification steps such as anion exchange chromatography. In addition, contamination with cellular DNA creates a therapeutic risk. Regulatory authorities require that DNA levels in all therapeutic protein and antibody samples be reduced to 10–100 pg/dose. Cell culture clarification processes, such a ...
... purification steps such as anion exchange chromatography. In addition, contamination with cellular DNA creates a therapeutic risk. Regulatory authorities require that DNA levels in all therapeutic protein and antibody samples be reduced to 10–100 pg/dose. Cell culture clarification processes, such a ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.