Chapter 1: Bio Primer - Columbia CS
... Ab-initio: compute structure directly from sequence Homology techniques: use similarity to known proteins ...
... Ab-initio: compute structure directly from sequence Homology techniques: use similarity to known proteins ...
Original Article Detection of Mycoplasma
... Department of Histology and Embryology, 2Department of Oncology and Radiotherapy, Charles University – Faculty of Medicine in Hradec Králové ...
... Department of Histology and Embryology, 2Department of Oncology and Radiotherapy, Charles University – Faculty of Medicine in Hradec Králové ...
Bio Frames - Lee County School District
... Define a problem based on a specific body of knowledge for example: biology, chemistry, physics, and earth/space science and do the following: (DOK High) (1) pose quesons about the natural world, (2) conduct systemic observa ons, (3) examine books and other sources of informa on to see what is already ...
... Define a problem based on a specific body of knowledge for example: biology, chemistry, physics, and earth/space science and do the following: (DOK High) (1) pose quesons about the natural world, (2) conduct systemic observa ons, (3) examine books and other sources of informa on to see what is already ...
Biomolecular chemistry 3. Translating the genetic code
... opposed to RNA, level) which may or may not be in the correct frame. If the next ATG encodes an in frame methionine, the translated protein will be missing the N-terminal sequence between its first two methionine residues. If the next ATG is out of frame (see +2 translation) then a nonsensical prote ...
... opposed to RNA, level) which may or may not be in the correct frame. If the next ATG encodes an in frame methionine, the translated protein will be missing the N-terminal sequence between its first two methionine residues. If the next ATG is out of frame (see +2 translation) then a nonsensical prote ...
Brief Rapid Communications
... therapy. First, this vector can only accept transgenes less than '4.5 kb in length. Second, current techniques do not allow the convenient production of large amounts of rAAV. Finally, although our studies have demonstrated efficient transduction of cardiomyocytes after 15 minutes of coronary artery ...
... therapy. First, this vector can only accept transgenes less than '4.5 kb in length. Second, current techniques do not allow the convenient production of large amounts of rAAV. Finally, although our studies have demonstrated efficient transduction of cardiomyocytes after 15 minutes of coronary artery ...
Biochemistry Syllabus
... 1. I can explain how the change in the structure of a molecular system may result in a change of the function of the system. 2. I can explain how the shape of enzymes, active sites and interaction with specific molecules are essential for basic functioning of the enzyme. a. I can explain how for an ...
... 1. I can explain how the change in the structure of a molecular system may result in a change of the function of the system. 2. I can explain how the shape of enzymes, active sites and interaction with specific molecules are essential for basic functioning of the enzyme. a. I can explain how for an ...
An enzyme within the ribosome catalyzes a synthesis reaction to
... 1. To start protein synthesis, a ribosome binds to mRNA. The ribosome has two binding sites for tRNA with its amino acid. Note that the first codon to associate with a tRNA is AUG, the start codon, which codes for methionine. The codon of mRNA and the anitcodon of tRNA are aligned and joined. The ot ...
... 1. To start protein synthesis, a ribosome binds to mRNA. The ribosome has two binding sites for tRNA with its amino acid. Note that the first codon to associate with a tRNA is AUG, the start codon, which codes for methionine. The codon of mRNA and the anitcodon of tRNA are aligned and joined. The ot ...
PKCa and PKCe differentially regulate Legionella pneumophila-induced GM-CSF
... spp. [20], and Pneumocystis jiroveci [21]. Furthermore, AMs from GM-CSF -/- mice were defective for Escherichia coli phagocytosis [18] as well as in the production of tumour necrosis factor (TNF)-a [22] and IFN-c [23]. The expression of GM-CSF is controlled by a tight regulatory network involving th ...
... spp. [20], and Pneumocystis jiroveci [21]. Furthermore, AMs from GM-CSF -/- mice were defective for Escherichia coli phagocytosis [18] as well as in the production of tumour necrosis factor (TNF)-a [22] and IFN-c [23]. The expression of GM-CSF is controlled by a tight regulatory network involving th ...
Vacuolar system distribution in Arabidopsis
... ¯uorescent ER while the other has already a ¯uorescent LV (e). Aleu-GFP ¯uorescence is faint, but labels the LV from early development, PVCs were also visible. A stronger ¯uorescence is observed in stomata located on the upper face of the leaf (f). Trichomes accumulate GFP-KDEL in the ER (g), GFP-Ch ...
... ¯uorescent ER while the other has already a ¯uorescent LV (e). Aleu-GFP ¯uorescence is faint, but labels the LV from early development, PVCs were also visible. A stronger ¯uorescence is observed in stomata located on the upper face of the leaf (f). Trichomes accumulate GFP-KDEL in the ER (g), GFP-Ch ...
PPT - Altogen Biosystems
... Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection If the viability of H9c2 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be ...
... Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection If the viability of H9c2 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be ...
Protein Synthesis and the Stress Response
... the fitness of E. coli in such oxidative conditions. It has also been shown that deletion of several tRNA modification enzymes affect survival of E. coli in a milder oxidative stress condition (0.5 mM H2O2) [56]. Despite these reports, it is not clear how tRNA modifications improve survival to oxida ...
... the fitness of E. coli in such oxidative conditions. It has also been shown that deletion of several tRNA modification enzymes affect survival of E. coli in a milder oxidative stress condition (0.5 mM H2O2) [56]. Despite these reports, it is not clear how tRNA modifications improve survival to oxida ...
truChIP™ Low Cell Chromatin Shearing Kit with Non-ionic
... 1. Thaw crosslinked cells on ice. 2. Add 1.0 ml Covaris Lysis Buffer (B) containing protease inhibitors. 3. Incubate for 10 minutes on a rocker (or equivalent) at 4°C. 4. Pellet nuclei by spinning at 1,700 x g for 5 minutes at 4°C. Carefully remove and discard the supernatant so as not to disturb t ...
... 1. Thaw crosslinked cells on ice. 2. Add 1.0 ml Covaris Lysis Buffer (B) containing protease inhibitors. 3. Incubate for 10 minutes on a rocker (or equivalent) at 4°C. 4. Pellet nuclei by spinning at 1,700 x g for 5 minutes at 4°C. Carefully remove and discard the supernatant so as not to disturb t ...
ATRX has a critical and conserved role in mammalian sexual differentiation
... Background: X-linked alpha thalassemia, mental retardation syndrome in humans is a rare recessive disorder caused by mutations in the ATRX gene. The disease is characterised by severe mental retardation, mild alphathalassemia, microcephaly, short stature, facial, skeletal, genital and gonadal abnorm ...
... Background: X-linked alpha thalassemia, mental retardation syndrome in humans is a rare recessive disorder caused by mutations in the ATRX gene. The disease is characterised by severe mental retardation, mild alphathalassemia, microcephaly, short stature, facial, skeletal, genital and gonadal abnorm ...
AAV User Manual - Vigene Biosciences
... Which viral vector to use- viral vector selection guide When comparing three most popular viral vectors in gene delivery, you should take following consideration before you choose adeno-associated viral vector. 1. Do you need transient or stable gene expression? 2. Do you need to transduce d ...
... Which viral vector to use- viral vector selection guide When comparing three most popular viral vectors in gene delivery, you should take following consideration before you choose adeno-associated viral vector. 1. Do you need transient or stable gene expression? 2. Do you need to transduce d ...
Viruses The Viruses
... Introduction and general characteristics • Virus : simple, acellular entities consisting of one or more molecules of either DNA or RNA enclosed in a coat of protein. They are reproduced only within living cells and are obligately intracellular parasites • The nucleic acid strands can be linear, clos ...
... Introduction and general characteristics • Virus : simple, acellular entities consisting of one or more molecules of either DNA or RNA enclosed in a coat of protein. They are reproduced only within living cells and are obligately intracellular parasites • The nucleic acid strands can be linear, clos ...
Phospholipase A2 s in Cell Injury and Death
... bromoenol lactone (BEL, a specific inhibitor of iPLA2) then it is likely that cPLA2 but not iPLA2 isoforms are involved in the process (Balsinde et al., 1999). The reason most inhibitors of cPLA2 can inhibit iPLA2 is both isoforms have a serine in their active site and these inhibitors contain a ser ...
... bromoenol lactone (BEL, a specific inhibitor of iPLA2) then it is likely that cPLA2 but not iPLA2 isoforms are involved in the process (Balsinde et al., 1999). The reason most inhibitors of cPLA2 can inhibit iPLA2 is both isoforms have a serine in their active site and these inhibitors contain a ser ...
CRISPR/Cas9 as a tool for creation of p53 knock
... successfully optimized a CRISPR mediated p53 knockout procedure for human glioma cells, and that such a feat had laid the groundwork for a future experiment that focused on knocking-in wtp53 to prevent and reverse dysregulation of glioma cell. This translates to a major step in curing cancers in wh ...
... successfully optimized a CRISPR mediated p53 knockout procedure for human glioma cells, and that such a feat had laid the groundwork for a future experiment that focused on knocking-in wtp53 to prevent and reverse dysregulation of glioma cell. This translates to a major step in curing cancers in wh ...
Supplementary figure legend
... (measured by qRT-PCR) and protein expression (measured by western blotting) levels (D). Western blotting was performed at least three times with three difference batches of breast cancer cell lines grown under basal growth conditions. Data is depicted as relative fold change normalized to MCF-7 cell ...
... (measured by qRT-PCR) and protein expression (measured by western blotting) levels (D). Western blotting was performed at least three times with three difference batches of breast cancer cell lines grown under basal growth conditions. Data is depicted as relative fold change normalized to MCF-7 cell ...
The Construction of GFP Fusion Genes For Transgenically Labeled
... clone 239-9P is 13.5 kb at minimum. Fragments in the 2-13 kb size range are expected to be large enough to contain all necessary cis-acting DNA sequences to recapitulate GFAP gene expression. This expectation is based on the finding that a 2.2 kb 5'-flanking sequence from the human GFAP gene was suf ...
... clone 239-9P is 13.5 kb at minimum. Fragments in the 2-13 kb size range are expected to be large enough to contain all necessary cis-acting DNA sequences to recapitulate GFAP gene expression. This expectation is based on the finding that a 2.2 kb 5'-flanking sequence from the human GFAP gene was suf ...
Crossing borders to bind proteins—a new concept in protein
... much as four orders of magnitude higher than that of the small molecule and with increased selectivity [14]. The small organic molecule or peptide used as ligand, binds to its binding site, and the attached polypeptide finds and occupies the most favourable site of interaction on the protein surface ...
... much as four orders of magnitude higher than that of the small molecule and with increased selectivity [14]. The small organic molecule or peptide used as ligand, binds to its binding site, and the attached polypeptide finds and occupies the most favourable site of interaction on the protein surface ...
Lipase Production from Free Cells and Immobilized Whole Cells
... catalytic triad is composed of Ser-Asp/Glu-his and usually also a consensus sequence.(GlyX-Ser-X-Gly) is found around the active site serine. The three dimensional structures of lipases reveal the characteristic α/β-hydrolase fold. (Nardini & Dijkstra 1999). However these enzymes also display cataly ...
... catalytic triad is composed of Ser-Asp/Glu-his and usually also a consensus sequence.(GlyX-Ser-X-Gly) is found around the active site serine. The three dimensional structures of lipases reveal the characteristic α/β-hydrolase fold. (Nardini & Dijkstra 1999). However these enzymes also display cataly ...
Warm up
... must keep in balance to maintain homeostasis. For each thing that is listed, describe how the body reacts when that thing is out of balance. • Cool down: Biology studies many different levels of life. List the level of life in order from smallest (cell) to largest (biosphere). For each level use an ...
... must keep in balance to maintain homeostasis. For each thing that is listed, describe how the body reacts when that thing is out of balance. • Cool down: Biology studies many different levels of life. List the level of life in order from smallest (cell) to largest (biosphere). For each level use an ...
GIAMPIERO CAI – curriculum vitae
... to specific destinations. Microtubule motors are classified as two superfamilies, the kinesins (further subdivided into 14 subfamilies) and dyneins. In previous years, major undertaken efforts were directed to identify proteins similar to kinesins in pollen tubes. This effort led to the characteriza ...
... to specific destinations. Microtubule motors are classified as two superfamilies, the kinesins (further subdivided into 14 subfamilies) and dyneins. In previous years, major undertaken efforts were directed to identify proteins similar to kinesins in pollen tubes. This effort led to the characteriza ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.