DNA Synthesis (Replication)
... to bridge the genetic code (AUCG) in mRNA with the twenty amino-acids code in proteins. Details of tRNA: The role of tRNA is to specify which sequence from the genetic code corresponds to which amino acid One end of the tRNA complements the genetic code in a threenucleotide sequence called the a ...
... to bridge the genetic code (AUCG) in mRNA with the twenty amino-acids code in proteins. Details of tRNA: The role of tRNA is to specify which sequence from the genetic code corresponds to which amino acid One end of the tRNA complements the genetic code in a threenucleotide sequence called the a ...
PART I. TUTORIAL QUESTIONS (30 marks total)
... vivo (i.e. in the living cell) Disadvantage: (1) The presence of GFP may present steric hindrance which may interfere with the function of the tagged protein or with the interactions that the tagged protein might be involved in. Also acceptable: usually requires overexpression of the protein of inte ...
... vivo (i.e. in the living cell) Disadvantage: (1) The presence of GFP may present steric hindrance which may interfere with the function of the tagged protein or with the interactions that the tagged protein might be involved in. Also acceptable: usually requires overexpression of the protein of inte ...
Answer any EIGHT questions from Section A. Each question carries
... Ans. Biotechnology (sometimes shortened to "biotech") is a field of applied biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bioproducts. Biotechnology also utilizes these products for manufacturing purpose. Modern use ...
... Ans. Biotechnology (sometimes shortened to "biotech") is a field of applied biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bioproducts. Biotechnology also utilizes these products for manufacturing purpose. Modern use ...
Nanotech Meets Microbiology
... arising from passive diffusion into cells. The behavior of single particles can also be tracked by attaching particles of interest to a bead, which serves as a handle for trapping the particles within optical tweezers and then tracking both the position of and forces acting on the bead and its attac ...
... arising from passive diffusion into cells. The behavior of single particles can also be tracked by attaching particles of interest to a bead, which serves as a handle for trapping the particles within optical tweezers and then tracking both the position of and forces acting on the bead and its attac ...
Genetic transfer in bioleaching microorganisms
... introduction of genetic material into cells. The three classical approaches are: transduction - the transfer of genetic information via a bacteriophage (virus) particle (Figure 1) conjugation - the transfer of conjugative or mobilizable plasmids from one bacterium to another by cell-to-cell cont ...
... introduction of genetic material into cells. The three classical approaches are: transduction - the transfer of genetic information via a bacteriophage (virus) particle (Figure 1) conjugation - the transfer of conjugative or mobilizable plasmids from one bacterium to another by cell-to-cell cont ...
midterm 2 asnwer scheme
... weak H bond will be disrupt and protein will unfold Mechanical stress – stirring & grinding actions disrupt the delicate balance of forces that maintain protein strcuture. eg. Foam formed when egg white is beaten vigorously contains denatured protein ...
... weak H bond will be disrupt and protein will unfold Mechanical stress – stirring & grinding actions disrupt the delicate balance of forces that maintain protein strcuture. eg. Foam formed when egg white is beaten vigorously contains denatured protein ...
1 INTRODUCTION Flow Cytometry involves the use of a beam of
... Computer Analysis: Following acquisition of data on the flow cytometer, analysis is undertaken to find out how many cells from the sampled population meet a criteria of interest. For example, does a population of cells express one molecule, but not another. The data can be displayed in a number of d ...
... Computer Analysis: Following acquisition of data on the flow cytometer, analysis is undertaken to find out how many cells from the sampled population meet a criteria of interest. For example, does a population of cells express one molecule, but not another. The data can be displayed in a number of d ...
Mechanisms of the Calcium Oscillation Inducing Toxins a
... specific combination of sugar and fat molecules). If the body senses LPS interacting with its cells it triggers its defence mechanisms, as it knows bacteria are present. LPS is a rather large structure with many sections. Here different sections of the LPS were removed to see whether a shorter or lo ...
... specific combination of sugar and fat molecules). If the body senses LPS interacting with its cells it triggers its defence mechanisms, as it knows bacteria are present. LPS is a rather large structure with many sections. Here different sections of the LPS were removed to see whether a shorter or lo ...
Depolymerization of Heparin/HS | NEB
... For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. ...
... For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. ...
Cell Division
... In eukaryotic cells, ________________ and associated ______________________are wrapped together in packages called chromosomes. DNA in eukaryotic cells is wrapped around the proteins to form a complex called______________________ Throughout the cell’s life, the chromatin becomes is loosely packed wi ...
... In eukaryotic cells, ________________ and associated ______________________are wrapped together in packages called chromosomes. DNA in eukaryotic cells is wrapped around the proteins to form a complex called______________________ Throughout the cell’s life, the chromatin becomes is loosely packed wi ...
mRNA translation
... The C-terminus end of the peptide in the P-site is coupled to the N-terminus of amino acid in the A-site mRNA binding site Conformational changes cause the shift of the mRNA by exactly three nucleotides so that a new AA-site becomes available ...
... The C-terminus end of the peptide in the P-site is coupled to the N-terminus of amino acid in the A-site mRNA binding site Conformational changes cause the shift of the mRNA by exactly three nucleotides so that a new AA-site becomes available ...
Help Wanted
... Positions Available in the genetics industry. Hundreds of entry-level openings for tireless workers. No previous experience necessary. Must be able to transcribe code in a nuclear environment. The ability to work in close association with ribosomes is a must. Accuracy and Speed vital for this job i ...
... Positions Available in the genetics industry. Hundreds of entry-level openings for tireless workers. No previous experience necessary. Must be able to transcribe code in a nuclear environment. The ability to work in close association with ribosomes is a must. Accuracy and Speed vital for this job i ...
Some words to think about
... • To replace dead or damaged cells • To grow and develop • To form a new life- fertilization birth ...
... • To replace dead or damaged cells • To grow and develop • To form a new life- fertilization birth ...
cis - Biology Courses Server
... Release of BiP from folded polypeptide requires energy (ATP)… Incorrectly folded proteins are held in ER until folded properly, or are targeted for degradation… ...
... Release of BiP from folded polypeptide requires energy (ATP)… Incorrectly folded proteins are held in ER until folded properly, or are targeted for degradation… ...
lecture notes-molecular biology-web
... Polyadenylation: a string of adenine nucleotides are added to the 3’ end. The string is several hundred nucleotides long. These two modifications are thought to increase mRNA stability and facilitate transport across the nuclear membrane. ...
... Polyadenylation: a string of adenine nucleotides are added to the 3’ end. The string is several hundred nucleotides long. These two modifications are thought to increase mRNA stability and facilitate transport across the nuclear membrane. ...
Excretion is the process in which _____ is (are) removed from the
... Heterozygous: situation where the two alleles at a specific genetic locus are not the same. Homologous: stretches of DNA that are very similar in sequence, so similar that they tend to stick together in hybridization experiments. Homologous can also be used to indicate related genes in separate orga ...
... Heterozygous: situation where the two alleles at a specific genetic locus are not the same. Homologous: stretches of DNA that are very similar in sequence, so similar that they tend to stick together in hybridization experiments. Homologous can also be used to indicate related genes in separate orga ...
Cell-free protein synthesis as a tool to study RXFP3- Relaxin
... production of the relaxin-family peptide receptor-3 (RXFP3), the cognate receptor for the neuropeptide relaxin-3. CFPS is a method whereby proteins are produced in an in-vitro environment by using cell extracts (S30 extract) derived from organisms such as Escherichia coli. S30 extracts contain all t ...
... production of the relaxin-family peptide receptor-3 (RXFP3), the cognate receptor for the neuropeptide relaxin-3. CFPS is a method whereby proteins are produced in an in-vitro environment by using cell extracts (S30 extract) derived from organisms such as Escherichia coli. S30 extracts contain all t ...
Name Miss Papassara Sangtanoo Position Research assistant
... - Sangtanoo P., Choowongkomon K., Surat W., Nitisinprasert S., and Kubera A. (2014). Antimicrobial peptides of ...
... - Sangtanoo P., Choowongkomon K., Surat W., Nitisinprasert S., and Kubera A. (2014). Antimicrobial peptides of ...
INTRODUCTION
... key proteins during oocyte development that have been found to localize specific mRNAs within the oocyte. It is also likely to be involved in RNA localization in other cell types, and for more general cellular functions. For instance, it has been found that human Trailerhitch localizes to P bodies ( ...
... key proteins during oocyte development that have been found to localize specific mRNAs within the oocyte. It is also likely to be involved in RNA localization in other cell types, and for more general cellular functions. For instance, it has been found that human Trailerhitch localizes to P bodies ( ...
Protocol for Phage T1-Resistant TransforMax™ EC100™
... 1. DNA should be in water or very low salt buffer (e.g. TE Buffer: 10 mM Tris-HCl [pH 7.5], 1 mM EDTA) to prevent arcing during electroporation. The pUC19 Control DNA is provided in TE at 100 pg/μl. If running a transformation control, dilute the pUC19 Control DNA 1:10 (to a final concentration ...
... 1. DNA should be in water or very low salt buffer (e.g. TE Buffer: 10 mM Tris-HCl [pH 7.5], 1 mM EDTA) to prevent arcing during electroporation. The pUC19 Control DNA is provided in TE at 100 pg/μl. If running a transformation control, dilute the pUC19 Control DNA 1:10 (to a final concentration ...
Translation
... controlled at all of these steps: •DNA packaging •Transcription •RNA processing and transport •RNA degradation •Translation •Post-translational Fig 16.1 ...
... controlled at all of these steps: •DNA packaging •Transcription •RNA processing and transport •RNA degradation •Translation •Post-translational Fig 16.1 ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.