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DNA replication
DNA replication

... • Promoter. Unidirectional sequence upstream of the coding region (i.e., at 5' end on sense strand) that tells the RNA polymerase both where to start and on which strand to continue synthesis. E.g. TATA box. • Terminator. Regulatory DNA region signaling end of transcription, at 3' end . • Transcript ...
Nucleus
Nucleus

... • Law of complimentary base pairing allows building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • replication fork is point of separation of 2 strands ...
Nucleus - Maryville University
Nucleus - Maryville University

... • Law of complimentary base pairing allows building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • replication fork is point of separation of 2 strands ...
9-1
9-1

... 3)Copying – container is heated again and polymerases build new strands of DNA. Polymerases continue adding nucleotides until entire DNA segment has been copied. PCR uses four materials. 1)DNA to be copied 2)DNA polymerase 3)A, T, C, and G nucleotides 4)two primers *Each PCR cycle doubles the number ...
Genetics (4) - HCC Learning Web
Genetics (4) - HCC Learning Web

... • Law of complimentary base pairing allows building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • replication fork is point of separation of 2 strands ...
Transcription Translation Sheet
Transcription Translation Sheet

... ...
Biochemistry
Biochemistry

... Proteins are the most complex macromolecules in the cell. They are composed of linear polymers called polypeptides, which contain amino acids connected by peptide bonds. ...
Study Guide
Study Guide

... 7. Avery and his team isolated Griffith’s transforming principle and performed three tests ...
Understanding DNA Technology - Southern Beef Technology Services
Understanding DNA Technology - Southern Beef Technology Services

The Good, the bad and the ugly of Genetic Engineering
The Good, the bad and the ugly of Genetic Engineering

... • Giving cows extra copies of the growth hormone gene • Giving plants the gene that insects have to ward off other enemy insects • Giving mice the gene that jelly fish use to fluoresce ...
document
document

... – Amino acids come together to form proteins, based on the code in the mRNA – tRNAs facitilate by “carrying” amino acids to the ribosome – Codon-anticodon interactions – Formation of peptide bonds between amino acids – Process repeats until termination – Further protein modifications after translati ...
Written Transcript of this video lesson in English (PDF
Written Transcript of this video lesson in English (PDF

... protein  which  might  be  structural  for  growth,  an  enzyme,  hormone   or  antibodies.   Any  change  in  the  nitrogen  bases  in  DNA  could  cause  genetic   mutation,  since  it  changes  the  genetic  code  which  in  turn   ...
ppt link
ppt link

... New strand synthesis always in the 5’-3’ direction ...
Importance of genetics: A brief History: Types of Genetic Diseases
Importance of genetics: A brief History: Types of Genetic Diseases

... Homozygous: describes a genetic condition characterized by the presence of two identical alleles for a given gene. Heterozygous: describes a genetic condition characterized by the presence of two different alleles for a given gene; the individual contains one dominant and one recessive allele in a g ...
Gene!
Gene!

... FC 0) in the Bl segment of the B cistron. Thie mutant was originally produced by the action of proflavins. We@ have previously argued that acridines such aa pro5vin act as mutagens because they add or dslsts a base or bases. The most striking evidence in favour of this is that mutants produced by a& ...
Written Transcript of this video lesson in English
Written Transcript of this video lesson in English

... which might be structural for growth, an enzyme, hormone or antibodies. Any change in the nitrogen bases in DNA could cause genetic mutation, since it changes the genetic code which in turn changes the amino acid composition and consequently changes the composition of the resulting protein which is ...
Decode the following message.
Decode the following message.

... removed from a DNA sequence at single point. • An deletion of one base pair causes a shift in the reading frame = One or more amino acids changed Base Pair Removed ...
Module 5 revised
Module 5 revised

...  has two strands-forming a “double helix”--held together by bonds between pairs of nucleotides ...
2. Be sure that your exam has 9 pages including this cover sheet.
2. Be sure that your exam has 9 pages including this cover sheet.

... Choose the best answer for the question or the best ending for each statement. Write the letter (A-E) which corresponds to the best answer on the line before the question. (2 pts each) _____1. A key point in Darwin's explanation of evolution is that A. biological structures most likely inherited are ...
Last Name: First Name: Per. _____ Parent Signature: Pre
Last Name: First Name: Per. _____ Parent Signature: Pre

... copying of the information stored in the DNA molecule of a gene into a mRNA molecule and this occurs in the nucleus. Translation occurs in the cytoplasm or on the E.R. on a ribosome and involves tRNA molecules bringing amino acids in the correct order (because each tRNA anticodon corresponds to a mR ...
What is another name for a polypeptide?
What is another name for a polypeptide?

... A mutation is a change in the nucleotide sequence of DNA. And as you can probably guess, a change in DNA leads to a change in mRNA, which can lead to a change in protein synthesis. ...
Genética Molecular em Medicina Transfusional
Genética Molecular em Medicina Transfusional

... of single-stranded DNA 20-30 nt long that hybridizes to one strand of the template DNA. • The rxn is intitiated by heating until the two strands of DNA separate, then the primers anneals to the complementary template strand, and DNA polymerase elongates the primer. ...
Recombinant Technology
Recombinant Technology

... Recombinant DNA technology utilizes the power of microbiological selection and screening procedures to allow investigators to isolate a gene that represents as little as 1 part in a million of the genetic material in an organism. The DNA from the organism of interest is divided into small pieces tha ...
90718-exm-04
90718-exm-04

... Determine the mutations in a variable region on the mitochondrial DNA. Establish the rate of mutation and thus, the rate of evolution of the Adelie penguins ...
GENETICS 310-PRINCIPLES OF HEREDITY
GENETICS 310-PRINCIPLES OF HEREDITY

... EXTRAS: Lecture notes, study guides (learning objectives) and PDF versions of old tests with and without answers can be accessed via the internet at: Genetics 310 TAMU . GRADES: Your grade will be determined by your performance on 3 in-class exams, a comprehensive final, and an outside paper on a re ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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