Protein Synthesis Bead Activity
... __________________________________ and it occurs in the ______________________ of cells. mRNA leaves the nucleus to find a _______________. Next, we start the second part of protein synthesis called _____________________________ and it happens in the _____________________ of cells. During this proce ...
... __________________________________ and it occurs in the ______________________ of cells. mRNA leaves the nucleus to find a _______________. Next, we start the second part of protein synthesis called _____________________________ and it happens in the _____________________ of cells. During this proce ...
Thermo Scientific Verso cDNA Kit
... RNA Priming It is recommended that RNA primers be added to the final 1X reaction as follows: 1 µL of anchored oligo-dT (orange cap) or 1 µL of random hexamers (blue cap) or 1 µL of a blend of random hexamers and anchored oligo-dT 3:1 (v/v) or gene-specific primer (to final concentration of 0.5 – 2 µ ...
... RNA Priming It is recommended that RNA primers be added to the final 1X reaction as follows: 1 µL of anchored oligo-dT (orange cap) or 1 µL of random hexamers (blue cap) or 1 µL of a blend of random hexamers and anchored oligo-dT 3:1 (v/v) or gene-specific primer (to final concentration of 0.5 – 2 µ ...
Mutation Activity
... DNA molecule except that the base T is replaced with the base U and RNA is single stranded (one half of the ladder). At the ribosome, another type of RNA (tRNA) transfers amino acids from the cytoplasm to the growing amino acid chain at the ribosome. BUT, sometimes there are problems with the DNA mo ...
... DNA molecule except that the base T is replaced with the base U and RNA is single stranded (one half of the ladder). At the ribosome, another type of RNA (tRNA) transfers amino acids from the cytoplasm to the growing amino acid chain at the ribosome. BUT, sometimes there are problems with the DNA mo ...
Biotechnology and Recombinant DNA
... interest from its genomic source and putting it in an expression vector. Steps: 1. Obtain the gene (PCR, restriction digest) 2. Ligate it into a vector (vector = carrier piece of DNA) 3. Transform the new recombinant DNA into bacteria/cells 4. Grow up a population of transformed cells that contain t ...
... interest from its genomic source and putting it in an expression vector. Steps: 1. Obtain the gene (PCR, restriction digest) 2. Ligate it into a vector (vector = carrier piece of DNA) 3. Transform the new recombinant DNA into bacteria/cells 4. Grow up a population of transformed cells that contain t ...
Biochemistry Review Game
... characteristic of one (or more) of the biomolecules. • You will need to be the first group to hold up the correct white board in order to get points! ...
... characteristic of one (or more) of the biomolecules. • You will need to be the first group to hold up the correct white board in order to get points! ...
Introduction to Next Generation Sequencing
... • Moores Law: Advances in technology are driving the ability to address questions on a genomic scale • Optimized Array Design Achievable – Requires Control Spike-In Data for Changes in Assay and Oligo Synthesis Approaches – Time consuming and costly • High Throughput Sequencing (Unbiased Functional ...
... • Moores Law: Advances in technology are driving the ability to address questions on a genomic scale • Optimized Array Design Achievable – Requires Control Spike-In Data for Changes in Assay and Oligo Synthesis Approaches – Time consuming and costly • High Throughput Sequencing (Unbiased Functional ...
Understanding the ABC of DNA technology
... The bacteria use the nutrients in the growth medium as food and will multiply rapidly. Different media are available depending on the preferred diet of the bacterial strain. Bacteria multiply by dividing: each individual bacterium can produce two more daughter bacteria. This leads to exponential pop ...
... The bacteria use the nutrients in the growth medium as food and will multiply rapidly. Different media are available depending on the preferred diet of the bacterial strain. Bacteria multiply by dividing: each individual bacterium can produce two more daughter bacteria. This leads to exponential pop ...
Structure of the Genome
... Packing: The tight packaging around the histones can only be achieved because the histones shield the negative phosphates from each other. Otherwise the DNA would repel itself and could not bend. The tight packing can make the DNA more inaccessible to transcription. Transcription factors which need ...
... Packing: The tight packaging around the histones can only be achieved because the histones shield the negative phosphates from each other. Otherwise the DNA would repel itself and could not bend. The tight packing can make the DNA more inaccessible to transcription. Transcription factors which need ...
Ch.11 Heredity
... A change in genetic code through natural selection, mutations, genetic drift A change in the ecosystem Over time: slow or rapid, or punctuated Common Ancestors ...
... A change in genetic code through natural selection, mutations, genetic drift A change in the ecosystem Over time: slow or rapid, or punctuated Common Ancestors ...
PPT Blank
... lactase digests lactose • VERY shape specific (“lock and key”) reaction with active site on enzyme (where substrate and enzyme join) One Enzyme : One Substrate •Enzymes have unique three-dimensional shapes so they can fit onto their specific substrate •Shapes determine function and which chemical re ...
... lactase digests lactose • VERY shape specific (“lock and key”) reaction with active site on enzyme (where substrate and enzyme join) One Enzyme : One Substrate •Enzymes have unique three-dimensional shapes so they can fit onto their specific substrate •Shapes determine function and which chemical re ...
ANSWER
... A. DNA – RNA – protein B. protein – RNA – DNA C. DNA – protein – RNA D. RNA – protein – DNA ...
... A. DNA – RNA – protein B. protein – RNA – DNA C. DNA – protein – RNA D. RNA – protein – DNA ...
Chapter 7: Microbial Genetics 10/8/2015
... Levels of Gene Regulation The expression of a gene into functional gene products can be regulated at multiple levels: TRANSCRIPTION* (regulation of rate at which gene is transcribed) ...
... Levels of Gene Regulation The expression of a gene into functional gene products can be regulated at multiple levels: TRANSCRIPTION* (regulation of rate at which gene is transcribed) ...
Genetics Review Sheet
... Resources: Class notes, Flow Chart, practice notes from sentence activity. Outline the process of protein synthesis- what are the steps that occur? Where does protein synthesis start in the cell? Where is the protein actually made? What does mRNA stand for? What does tRNA stand for? Translate DNA in ...
... Resources: Class notes, Flow Chart, practice notes from sentence activity. Outline the process of protein synthesis- what are the steps that occur? Where does protein synthesis start in the cell? Where is the protein actually made? What does mRNA stand for? What does tRNA stand for? Translate DNA in ...
Photosynthesis - Cathedral High School
... Many mutagens are also carcinogens (cancer causing) Environmental Mutagens ...
... Many mutagens are also carcinogens (cancer causing) Environmental Mutagens ...
are we fully shaped and determined by our genes?
... First, the Aristotelian soul was conceived as something absolutely integrated and indivisible. Its masterpiece, the „body” can change from an imperfect into a perfect form. New body parts may appear or disappear, the body can be mutilated. But the soul itself is not a spatial being, therefore it doe ...
... First, the Aristotelian soul was conceived as something absolutely integrated and indivisible. Its masterpiece, the „body” can change from an imperfect into a perfect form. New body parts may appear or disappear, the body can be mutilated. But the soul itself is not a spatial being, therefore it doe ...
Chapter 17 – Molecular genetics
... Some amino acids (AA) are coded for by more than one codon, while others, only by one Each set of 3 amino acids is called a reading frame ...
... Some amino acids (AA) are coded for by more than one codon, while others, only by one Each set of 3 amino acids is called a reading frame ...
Electrophoresis
... A simple rapid, sensitive and versatile in vitro method for selectively amplifying defined sequences/regions of DNA/RNA from an initial complex source of nucleic acid - generates sufficient for subsequent analysis and/or manipulation Amplification of a small amount of DNA using specific DNA prim ...
... A simple rapid, sensitive and versatile in vitro method for selectively amplifying defined sequences/regions of DNA/RNA from an initial complex source of nucleic acid - generates sufficient for subsequent analysis and/or manipulation Amplification of a small amount of DNA using specific DNA prim ...
DNA - BiVDA
... are faithfully duplicated. The DNA strands are unwound and each parental strand is used as a template in the synthesis of a complementary strand. The new and old strands are then reformed into a tightly wound helix. Although the replication process has high fidelity, errors do occur at very low freq ...
... are faithfully duplicated. The DNA strands are unwound and each parental strand is used as a template in the synthesis of a complementary strand. The new and old strands are then reformed into a tightly wound helix. Although the replication process has high fidelity, errors do occur at very low freq ...
DNA Structure and DNA Replication Practice Problems
... DNA Replication. Use the hypothetical representation of a double stranded DNA molecule, below, to complete the following tasks. a. Complete the base sequence of the complementary strand of the hypothetical DNA molecule diagrammed below. b. Label the 5’ and 3’ ends of each strand. c. Use dashed lines ...
... DNA Replication. Use the hypothetical representation of a double stranded DNA molecule, below, to complete the following tasks. a. Complete the base sequence of the complementary strand of the hypothetical DNA molecule diagrammed below. b. Label the 5’ and 3’ ends of each strand. c. Use dashed lines ...
Biomolecule Test Review 2015
... 9. What is the difference between saturated and unsaturated fatty acid? Which is better for you? Why? Saturated fatty acid- single bonds, straight and tightly packed. Solid at room temperature. (Bad for us!) Unsaturated fatty acid- double bonds bend the tails and it’s crooked (not straight). Liquid ...
... 9. What is the difference between saturated and unsaturated fatty acid? Which is better for you? Why? Saturated fatty acid- single bonds, straight and tightly packed. Solid at room temperature. (Bad for us!) Unsaturated fatty acid- double bonds bend the tails and it’s crooked (not straight). Liquid ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.