Multiple Sequence Alignments and Sequence Profiles
... – See course website for many more listings… ...
... – See course website for many more listings… ...
The epidermal intermediate filament proteins of
... The cDNA sequence of S. clava B protein (Fig. 1; accession number AJ271144) predicts a protein of molecular weight 51 923 with an isoelectric point of 5.02, which extends at the Nterminal side the sequence previously given for the S. plicata protein (Riemer and Weber, 1998). Reinvestigating the cDNA ...
... The cDNA sequence of S. clava B protein (Fig. 1; accession number AJ271144) predicts a protein of molecular weight 51 923 with an isoelectric point of 5.02, which extends at the Nterminal side the sequence previously given for the S. plicata protein (Riemer and Weber, 1998). Reinvestigating the cDNA ...
Poster
... inhibit PTP1B inside cells. LZP25 avoids this issue by not having a formal negative charge, but instead a polar area of similar size to phosphate. Binding to the PTP1B active site pocket (sites Ser216, Ala217, Ile219, Gln262, Gln266), its bulky side groups then prevent a key loop in the enzyme activ ...
... inhibit PTP1B inside cells. LZP25 avoids this issue by not having a formal negative charge, but instead a polar area of similar size to phosphate. Binding to the PTP1B active site pocket (sites Ser216, Ala217, Ile219, Gln262, Gln266), its bulky side groups then prevent a key loop in the enzyme activ ...
Methods and approaches for the comprehensive characterization
... amino acids in cell culture (SILAC). Cells are cultured in a medium supplemented with a labeled amino acid (lysine or arginine containing 13C or 15N). After several passages, cells are pooled with control cells grown in a medium of naturally occurring amino acids. The pooled samples are digested and ...
... amino acids in cell culture (SILAC). Cells are cultured in a medium supplemented with a labeled amino acid (lysine or arginine containing 13C or 15N). After several passages, cells are pooled with control cells grown in a medium of naturally occurring amino acids. The pooled samples are digested and ...
Xanthomonas campestris
... (Supporting Information Table 3). It was interesting to observe a lower diversity of the bacterial proteins identified in REU as well as a higher amount of plant proteins in this sample. Similar results were obtained by Villeth et al. [13] when analyzing Xcc recovered from the resistant plant (REU) ...
... (Supporting Information Table 3). It was interesting to observe a lower diversity of the bacterial proteins identified in REU as well as a higher amount of plant proteins in this sample. Similar results were obtained by Villeth et al. [13] when analyzing Xcc recovered from the resistant plant (REU) ...
Vicia species belonging to the subgenus Cracca are
... RC: 4. The language of the manuscript is too weak which needs to be edited by native language professional for grammatical and syntax errors. AA: The language has been now revised by some one familiar with english ...
... RC: 4. The language of the manuscript is too weak which needs to be edited by native language professional for grammatical and syntax errors. AA: The language has been now revised by some one familiar with english ...
Organic Chemistry
... 1) Order of amino acids 2) Initial folding (helix, sheets) 3) Folding of folds (globular) 4) Multiple polypeptide chains together ...
... 1) Order of amino acids 2) Initial folding (helix, sheets) 3) Folding of folds (globular) 4) Multiple polypeptide chains together ...
practice exam
... molecule that you store in your liver. Circle the pathways/cycles below that are part of this overall transformation. Cross out any that are not. citric acid cycle, gluconeogenesis, pentose phosphate pathway, glycogen synthesis, glycolysis B. Trace the metabolic path of this glutamate molecule throu ...
... molecule that you store in your liver. Circle the pathways/cycles below that are part of this overall transformation. Cross out any that are not. citric acid cycle, gluconeogenesis, pentose phosphate pathway, glycogen synthesis, glycolysis B. Trace the metabolic path of this glutamate molecule throu ...
Nutritional Considerations
... Athletes should consume carbohydrates after a workout to maximize recovery if time between exercise sessions is less than 8 hours. ...
... Athletes should consume carbohydrates after a workout to maximize recovery if time between exercise sessions is less than 8 hours. ...
Master Entrance Exam
... 5. Which amino acid has hydrophobic side chain (A) Asparagine (B) Isoleucine (C) Lysine (D) Threonine (E) Proline 6. Product of fermentation in muscle cells (A) Glucose (B) Lactate (C) Glycerol (D) Fructose (E) Ribose 7. Compound that connects the urea cycle to the citrate cycle (is involved in both ...
... 5. Which amino acid has hydrophobic side chain (A) Asparagine (B) Isoleucine (C) Lysine (D) Threonine (E) Proline 6. Product of fermentation in muscle cells (A) Glucose (B) Lactate (C) Glycerol (D) Fructose (E) Ribose 7. Compound that connects the urea cycle to the citrate cycle (is involved in both ...
putative mineral-specific proteins synthesized by a
... needed. In-gel tryptic digestion and mass spectrometry (MS) was performed by the Michigan Proteome Consortium (University of Michigan, Ann Arbor, Michigan) using a 4700 Proteomic MALDI TOF-TOF Analyzer (Applied Biosystems, Framingham, California). For protein identification, we preformed both automa ...
... needed. In-gel tryptic digestion and mass spectrometry (MS) was performed by the Michigan Proteome Consortium (University of Michigan, Ann Arbor, Michigan) using a 4700 Proteomic MALDI TOF-TOF Analyzer (Applied Biosystems, Framingham, California). For protein identification, we preformed both automa ...
Chapter 10 Notes
... The Bacteriorhodopsin story • Halobacterium halobium, the salt-loving bacterium, carries out normal respiration if O2 and substrates are plentiful • But when substrates are lacking, it can survive by using bacteriorhodopsin and halorhodopsin to capture light energy • Purple patches of H. halobium ar ...
... The Bacteriorhodopsin story • Halobacterium halobium, the salt-loving bacterium, carries out normal respiration if O2 and substrates are plentiful • But when substrates are lacking, it can survive by using bacteriorhodopsin and halorhodopsin to capture light energy • Purple patches of H. halobium ar ...
Lateral gene transfer and the evolution of plastid
... To infer the evolutionary histories of the 78 B. natans plastidtargeted proteins, amino acid sequences were aligned with homologs from other organisms, and phylogenetic analyses were performed. ML-distance trees were inferred, and bootstrap analyses (500 replicates) were performed for all 78 dataset ...
... To infer the evolutionary histories of the 78 B. natans plastidtargeted proteins, amino acid sequences were aligned with homologs from other organisms, and phylogenetic analyses were performed. ML-distance trees were inferred, and bootstrap analyses (500 replicates) were performed for all 78 dataset ...
Text S1 Y2H Interactome Mapping The literature was curated to
... reasons because of the high degree of similarity between Esx proteins. The list was further curated to remove ORFs in functional category 1 (lipid metabolism), 2 (information pathway), and 7 (intermediary metabolism and respiration) unless they had possible signal sequences, possible TAT-dependent ...
... reasons because of the high degree of similarity between Esx proteins. The list was further curated to remove ORFs in functional category 1 (lipid metabolism), 2 (information pathway), and 7 (intermediary metabolism and respiration) unless they had possible signal sequences, possible TAT-dependent ...
Enzymes - SAVE MY EXAMS!
... (ii) State the maximum number of amino acids coded for by this length of mRNA. ...
... (ii) State the maximum number of amino acids coded for by this length of mRNA. ...
Introduction to Carbohydrates
... by proteases from the pancreas. The peptide bonds susceptible to hydrolysis are shown for each of the five major pancreatic proteases. [Note: Enteropeptidaseis synthesized in the intestine.] ...
... by proteases from the pancreas. The peptide bonds susceptible to hydrolysis are shown for each of the five major pancreatic proteases. [Note: Enteropeptidaseis synthesized in the intestine.] ...
Biology
... What are the levels of protein structure? 1. Primary (1o) – amino acid sequence; this determines the next 3 levels 2. Secondary (2o) – the amino acid chains coils and folds 3. Tertiary (3o) – the 2o structure folds on itself 4. Quaternary (4o) – one or more polypeptide chains are added; a.k.a. globu ...
... What are the levels of protein structure? 1. Primary (1o) – amino acid sequence; this determines the next 3 levels 2. Secondary (2o) – the amino acid chains coils and folds 3. Tertiary (3o) – the 2o structure folds on itself 4. Quaternary (4o) – one or more polypeptide chains are added; a.k.a. globu ...
Diversity and Formation of Endoplasmic Reticulum
... et al., 2001). All the compartments that have been characterized share three common features: (1) The ER-derived compartments accumulate a large amount of a single protein or only a few different proteins. (2) The protein components do not act within the compartments, even if they are functional pro ...
... et al., 2001). All the compartments that have been characterized share three common features: (1) The ER-derived compartments accumulate a large amount of a single protein or only a few different proteins. (2) The protein components do not act within the compartments, even if they are functional pro ...
E. coli
... Differentiate between an endotoxin and an exotoxin as far as source, chemistry and type of molecule (protein, or polysaccharide/lipid). List and understand how examples from class work (e.g., cytotoxin, hemolysin, neurotoxin, enterotoxin, endotoxin). It is not necessary to know the particular detail ...
... Differentiate between an endotoxin and an exotoxin as far as source, chemistry and type of molecule (protein, or polysaccharide/lipid). List and understand how examples from class work (e.g., cytotoxin, hemolysin, neurotoxin, enterotoxin, endotoxin). It is not necessary to know the particular detail ...
The Production, Purification, and Bioactivity of Recombinant Bovine
... M Tris-HCI buffer, pH 8.0, at - 2 0 C for 6 months. Activity was not significantly reduced by four cycles of freezing and thawing. Activity was also retained when the protein was stored at 4 C for 2 weeks, or exposed to acidic conditions (pH 2.0; 30 min). This stability along with ease of purificati ...
... M Tris-HCI buffer, pH 8.0, at - 2 0 C for 6 months. Activity was not significantly reduced by four cycles of freezing and thawing. Activity was also retained when the protein was stored at 4 C for 2 weeks, or exposed to acidic conditions (pH 2.0; 30 min). This stability along with ease of purificati ...
- Wiley Online Library
... clability are among the main reasons that have prompted the use of enzymes in industrial biocatalysis [2, 3]. All these particular properties displayed by enzymes are a consequence of their complex 3-D structure. In the native state, the polypeptide chain adopts such a conformation that the hydropho ...
... clability are among the main reasons that have prompted the use of enzymes in industrial biocatalysis [2, 3]. All these particular properties displayed by enzymes are a consequence of their complex 3-D structure. In the native state, the polypeptide chain adopts such a conformation that the hydropho ...
Allied Biochemistry II - E
... (1) proteins are denatured by the SDS (2) proteins have the same charge-to-mass ratio (3) smaller proteins migrate more rapidly through the gel (4) all of the above 26. Proteins can be visualized directly in gels by (1) staining them with the dye (2) using electron microscope only (3) measuring thei ...
... (1) proteins are denatured by the SDS (2) proteins have the same charge-to-mass ratio (3) smaller proteins migrate more rapidly through the gel (4) all of the above 26. Proteins can be visualized directly in gels by (1) staining them with the dye (2) using electron microscope only (3) measuring thei ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.