Phosphate binding sites identification in protein
... if at least three amino acids of the protein have an atom located closer than 4.0 Å from the phosphorus atom. The structures that did not fulfill this criterion were eliminated thus reducing the training set to 40 protein chains. Since this data set is focused on nucleotides, we added 20 protein str ...
... if at least three amino acids of the protein have an atom located closer than 4.0 Å from the phosphorus atom. The structures that did not fulfill this criterion were eliminated thus reducing the training set to 40 protein chains. Since this data set is focused on nucleotides, we added 20 protein str ...
Biochemistry - CEU Cardenal Herrera
... relationship with other subjects such as physiology, pathology and nutrition. In the first part of the course we will describe the structures of the major biomolecules and their physical and chemical properties, with particular emphasis on the structure / function. The second part is focused in the ...
... relationship with other subjects such as physiology, pathology and nutrition. In the first part of the course we will describe the structures of the major biomolecules and their physical and chemical properties, with particular emphasis on the structure / function. The second part is focused in the ...
HiPer® Affinity Chromatography Teaching Kit
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
HW #2
... 1. A two-bit wide, 2:1 mux selects between two inputs, A and B, each of which is two bits wide, placing one of the inputs onto the two bit output C. Given this interface (IN: A1 , A0 , B1 , B0 , S, OUT: C1 , C0 , draw a circuit implementing such a mux. 2. Show how F (ABC) = Σm(0, 2, 6, 7) can be imp ...
... 1. A two-bit wide, 2:1 mux selects between two inputs, A and B, each of which is two bits wide, placing one of the inputs onto the two bit output C. Given this interface (IN: A1 , A0 , B1 , B0 , S, OUT: C1 , C0 , draw a circuit implementing such a mux. 2. Show how F (ABC) = Σm(0, 2, 6, 7) can be imp ...
Untitled
... The plasma membrane separates the inside of the cell (cytoplasm) from the outside (or extracellular) environment. The shapes of lipids and the way they pack with each other have a large effect on the structures that they form in water, or water-based solutions (referred to as aqueous, or water-cont ...
... The plasma membrane separates the inside of the cell (cytoplasm) from the outside (or extracellular) environment. The shapes of lipids and the way they pack with each other have a large effect on the structures that they form in water, or water-based solutions (referred to as aqueous, or water-cont ...
Different transport mechanisms Aqueous diffusion Lipid
... Carrier-mediated transport • Transport across membrane utilizes membrane-bound proteins (carriers or transporters) • Facilitated transport (direction of transport down the conc. gradient) • Active transport (direction of transport up the conc. gradient) (requires ATP) • Since it is protein mediated ...
... Carrier-mediated transport • Transport across membrane utilizes membrane-bound proteins (carriers or transporters) • Facilitated transport (direction of transport down the conc. gradient) • Active transport (direction of transport up the conc. gradient) (requires ATP) • Since it is protein mediated ...
Macromolecular Sequence Analysis Biological sequences
... the protein chain takes a 180° change in direction, doubling back on itself. Such kind of hairpin turns are found for example between two adjacent β strands. The side chain R3 is usually H (glycine) ...
... the protein chain takes a 180° change in direction, doubling back on itself. Such kind of hairpin turns are found for example between two adjacent β strands. The side chain R3 is usually H (glycine) ...
Logistic regression models to predict solvent accessible residues
... of solvent accessible surfaces directly from sequence information, allowing for the identification of relevant clusters on the surface (Xue et al., 2011; Tygai et al., 2012). Other structural features may prove amenable to prediction from sequence, including specifically the identification of critic ...
... of solvent accessible surfaces directly from sequence information, allowing for the identification of relevant clusters on the surface (Xue et al., 2011; Tygai et al., 2012). Other structural features may prove amenable to prediction from sequence, including specifically the identification of critic ...
Biology is the only subject in which multiplication is the same thing
... gene codes for a protein channel that allows salt to flow across cell membrane broken protein doesn’t work as channel doesn’t allow salt out of cell, so water doesn’t flow out ...
... gene codes for a protein channel that allows salt to flow across cell membrane broken protein doesn’t work as channel doesn’t allow salt out of cell, so water doesn’t flow out ...
Metabolism of amino acid
... Copy from: http://themedicalbiochemistrypage.org/amino-acid-metabolism.html ...
... Copy from: http://themedicalbiochemistrypage.org/amino-acid-metabolism.html ...
F9550 - Datasheet - Sigma
... Incubate for 10 min. at 25 °C. Stop reactions by the addition 5 µl stop solution. Boil for 5 min. at 95 °C. Load 4 µl sample on the denaturing gel. Note: wash the wells before loading. Run the mini gel at 200V with circulating cold water (~10°C) to reduce heating until the stain front reaches 1-2 cm ...
... Incubate for 10 min. at 25 °C. Stop reactions by the addition 5 µl stop solution. Boil for 5 min. at 95 °C. Load 4 µl sample on the denaturing gel. Note: wash the wells before loading. Run the mini gel at 200V with circulating cold water (~10°C) to reduce heating until the stain front reaches 1-2 cm ...
No Slide Title
... Urea Cycle - regulation 1. By flux of nitrogen through cycle - depends on diet lots protein in diet = carbon skeletons used for fuel, lots of urea starvation = breakdown muscle protein for energy, lots of urea All enzymes (CPS-I and 4 in cycle) synthesized at higher rates in starving animals and ani ...
... Urea Cycle - regulation 1. By flux of nitrogen through cycle - depends on diet lots protein in diet = carbon skeletons used for fuel, lots of urea starvation = breakdown muscle protein for energy, lots of urea All enzymes (CPS-I and 4 in cycle) synthesized at higher rates in starving animals and ani ...
Amino Acid Metabolism - Breakdown Other metabolic
... Other metabolic pathways Urea Cycle - regulation 1. By flux of nitrogen through cycle - depends on diet lots protein in diet = carbon skeletons used for fuel, lots of urea starvation = breakdown muscle protein for energy, lots of urea All enzymes (CPS-I and 4 in cycle) synthesized at higher rates i ...
... Other metabolic pathways Urea Cycle - regulation 1. By flux of nitrogen through cycle - depends on diet lots protein in diet = carbon skeletons used for fuel, lots of urea starvation = breakdown muscle protein for energy, lots of urea All enzymes (CPS-I and 4 in cycle) synthesized at higher rates i ...
Protein Synthesis
... the sequence. Once the anticodon and codon sequences are bound (remember, they are complementary base pairs), the tRNA presents its amino acid cargo and the growing polypeptide strand is attached to this next amino acid. This attachment takes place with the assistance of various enzymes and requires ...
... the sequence. Once the anticodon and codon sequences are bound (remember, they are complementary base pairs), the tRNA presents its amino acid cargo and the growing polypeptide strand is attached to this next amino acid. This attachment takes place with the assistance of various enzymes and requires ...
The Tryptophan Mutant in the Human Immunodeficiency Virus Type
... as CD4+ T cells) and non-dividing/terminally differentiated cells (such as macrophages)8. An actively dividing cell refers to a cell which is actively going through the cell division process to replicate more copies of itself. In its replication process, HIV-1 undergoes a step-by-step life cycle. Fi ...
... as CD4+ T cells) and non-dividing/terminally differentiated cells (such as macrophages)8. An actively dividing cell refers to a cell which is actively going through the cell division process to replicate more copies of itself. In its replication process, HIV-1 undergoes a step-by-step life cycle. Fi ...
Enzymes
... 1. Enzymes speed up chemical reactions that take place in cells. 2. Enzymes are very specific, generally catalyzing only one chemical reaction. 3. For this reason, part of an enzyme’s name is usually derived from the reaction it catalyzes. Enzymes usually end in the suffix “–ase”. Ex. Alcohol dehydr ...
... 1. Enzymes speed up chemical reactions that take place in cells. 2. Enzymes are very specific, generally catalyzing only one chemical reaction. 3. For this reason, part of an enzyme’s name is usually derived from the reaction it catalyzes. Enzymes usually end in the suffix “–ase”. Ex. Alcohol dehydr ...
1 -2 -2 2 -3 I -1
... Galton left his home and headed for a country fair… He believed that only a very few people had the characteristics necessary to keep societies healthy. He had devoted much of his career to measuring those characteristics, in fact, in order to prove that the vast majority of people did not have them ...
... Galton left his home and headed for a country fair… He believed that only a very few people had the characteristics necessary to keep societies healthy. He had devoted much of his career to measuring those characteristics, in fact, in order to prove that the vast majority of people did not have them ...
corneagene_cm
... The immunohistochemically detectable BIGH3 amount was less than normal in keratoconus corneas, however, it increased when scarring was also present in the specimens. In the future, they would like to continue their investigations in two main directions: 1. Investigation of the DNA mutations occurrin ...
... The immunohistochemically detectable BIGH3 amount was less than normal in keratoconus corneas, however, it increased when scarring was also present in the specimens. In the future, they would like to continue their investigations in two main directions: 1. Investigation of the DNA mutations occurrin ...
Ten remarks on peptide bond formation on the ribosome
... primary amines predominantly exist in the protonated ammonium form, and the α-amino group has to be deprotonated to generate the nucleophilic NH2 group. Secondly, the putative T± intermediate [17] contains a positively charged secondary amine and an oxyanion linked to the tetrahedral carbon. Thirdly ...
... primary amines predominantly exist in the protonated ammonium form, and the α-amino group has to be deprotonated to generate the nucleophilic NH2 group. Secondly, the putative T± intermediate [17] contains a positively charged secondary amine and an oxyanion linked to the tetrahedral carbon. Thirdly ...
What are enzymes and how do they work
... c. Which of the following sequences within the mRNA most likely contains the ribosome binding site? (Circle ONE) ...
... c. Which of the following sequences within the mRNA most likely contains the ribosome binding site? (Circle ONE) ...
European Journal of Biochemistry
... should n o t grossly alter the conformation or localizatim of (Fig. I b). Subseqiiently. t h i s purified plasmid and p.iP79 D N A M C I W the protein. The following lines of evidence indicate that the wparately digested with Hglll and G o R I . Plasmid DX/\ fragments PhoE* protein is norinally fold ...
... should n o t grossly alter the conformation or localizatim of (Fig. I b). Subseqiiently. t h i s purified plasmid and p.iP79 D N A M C I W the protein. The following lines of evidence indicate that the wparately digested with Hglll and G o R I . Plasmid DX/\ fragments PhoE* protein is norinally fold ...
Enzymes I
... and heme). Organic cofactors can be either prosthetic groups, which are tightly bound to an enzyme, or coenzymes, which are released from the enzyme's active site during the reaction. Coenzymes include NADH, NADPH and adenosine triphosphate. These molecules transfer chemical groups between enzymes. ...
... and heme). Organic cofactors can be either prosthetic groups, which are tightly bound to an enzyme, or coenzymes, which are released from the enzyme's active site during the reaction. Coenzymes include NADH, NADPH and adenosine triphosphate. These molecules transfer chemical groups between enzymes. ...
A1982NK48200001
... communities and rates change. “Richard Wright and I, young postdoctoral fellows at Uppsala, started out by looking at algal uptake of low levels of sugars and organic acids. We had to modify the kinetic 1 approach of Parsons and Strickland for the mixed populations of lakes and for the parts-per-bil ...
... communities and rates change. “Richard Wright and I, young postdoctoral fellows at Uppsala, started out by looking at algal uptake of low levels of sugars and organic acids. We had to modify the kinetic 1 approach of Parsons and Strickland for the mixed populations of lakes and for the parts-per-bil ...
The Estimation of Kinetic Parameters in Systems - Beilstein
... One of the aims of systems biology is to provide a mathematical description of metabolic or signalling protein networks. This can be achieved by constructing a set of differential equations describing changes in concentrations of compounds with time [1]. Enzymespecific parameters, such as ligand bin ...
... One of the aims of systems biology is to provide a mathematical description of metabolic or signalling protein networks. This can be achieved by constructing a set of differential equations describing changes in concentrations of compounds with time [1]. Enzymespecific parameters, such as ligand bin ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.