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Questions for exam #1

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... The serine proteases • Proteases catalyze hydrolysis of peptide bonds • Mechanism of uncatalyzed reaction is simple nucleophilic attack by the :O of the H2O on the carbonyl C of the peptide bond, forming a tetrahedral intermediate which then breaks down as the amine "half" of the original peptide l ...
Mechanisms of catalysis
Mechanisms of catalysis

... The serine proteases • Proteases catalyze hydrolysis of peptide bonds • Mechanism of uncatalyzed reaction is simple nucleophilic attack by the :O of the H2O on the carbonyl C of the peptide bond, forming a tetrahedral intermediate which then breaks down as the amine "half" of the original peptide l ...
2005 MCB 3020 Study Objectives, Part 2
2005 MCB 3020 Study Objectives, Part 2

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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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