Patent Protection for the Protein Products of Recombinant DNA
... foregone conclusion since the recombinant protein will at least serve the same useful purpose as its naturally occurring counterpart. Furthermore, a first-generation recombinant protein may be considered novel and hence patentable despite the existence of the naturally occurring protein, so long as ...
... foregone conclusion since the recombinant protein will at least serve the same useful purpose as its naturally occurring counterpart. Furthermore, a first-generation recombinant protein may be considered novel and hence patentable despite the existence of the naturally occurring protein, so long as ...
Strong and weak hydrogen bonds in the protein
... HA) 908. For other weak interactions, the criteria are mentioned in the respective sections. A schematic description of the various interactions is given in Scheme 1. Macromolecular crystal structures rarely contain H-atom positional data with the precision required to properly evaluate hydroge ...
... HA) 908. For other weak interactions, the criteria are mentioned in the respective sections. A schematic description of the various interactions is given in Scheme 1. Macromolecular crystal structures rarely contain H-atom positional data with the precision required to properly evaluate hydroge ...
Cloning, Functional Characterization and Site
... of proteins in plants, and they show high similarity to true 4CL genes with a conserved AMP binding domain (Box I) and Box II domain (GEICIRG). Different from true 4CLs, 4CL-like enzymes commonly lack activity toward hydroxycinnamate substrates (p-coumaric, caffeic, ferulic, and sinapic acids) and m ...
... of proteins in plants, and they show high similarity to true 4CL genes with a conserved AMP binding domain (Box I) and Box II domain (GEICIRG). Different from true 4CLs, 4CL-like enzymes commonly lack activity toward hydroxycinnamate substrates (p-coumaric, caffeic, ferulic, and sinapic acids) and m ...
The Effects of Whey Protein and Leucine Supplementation
... weight (2) and hypertrophy (5,6), as well as result in favorable changes in body composition including decreases in percent body fat (%fat) and fat weight (FW), and increases in fat-free weight (FFW) (2, 7). Furthermore, the magnitude of the increases in strength and hypertrophy are related to the v ...
... weight (2) and hypertrophy (5,6), as well as result in favorable changes in body composition including decreases in percent body fat (%fat) and fat weight (FW), and increases in fat-free weight (FFW) (2, 7). Furthermore, the magnitude of the increases in strength and hypertrophy are related to the v ...
Reprogramming the Genetic Code: From Triplet to Quadruplet Codes
... There are several major challenges in genetically encoding multiple unnatural amino acids into proteins in living cells. First, unique new codons are required to encode the incorporation of unnatural amino acids at specific sites in proteins. Since the 64 triplet codons are already used in the genom ...
... There are several major challenges in genetically encoding multiple unnatural amino acids into proteins in living cells. First, unique new codons are required to encode the incorporation of unnatural amino acids at specific sites in proteins. Since the 64 triplet codons are already used in the genom ...
Quantitative Host Cell Protein Analysis Using Two
... content provided by ELISA analysis is currently acceptable for lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate identification of individual HCPs have gained prominence in recen ...
... content provided by ELISA analysis is currently acceptable for lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate identification of individual HCPs have gained prominence in recen ...
tutorial_structurech..
... All cis peptide bonds are regarded being unusual. In case of chiral centers, those are regarded unusual which occur less often in nature, e.g. D-form of amino acids. 2 Inspect the found irregularities Although it is possible to convert all peptide bonds and chirality centers into their “usual” confi ...
... All cis peptide bonds are regarded being unusual. In case of chiral centers, those are regarded unusual which occur less often in nature, e.g. D-form of amino acids. 2 Inspect the found irregularities Although it is possible to convert all peptide bonds and chirality centers into their “usual” confi ...
Regulation of Protein Degradation
... have very limited cleavage site specificities and produce detectable protein products. Although several of the proteasome cleaving activities can be related to the four classes of proteinase through the use of class-specific inhibitors and peptide substrates, none of the proteasome subunits shares a ...
... have very limited cleavage site specificities and produce detectable protein products. Although several of the proteasome cleaving activities can be related to the four classes of proteinase through the use of class-specific inhibitors and peptide substrates, none of the proteasome subunits shares a ...
PDB database http://www.pdb.org/
... Ligand and atom nomenclature. Residue and atom nomenclature is compared against the PDB dictionary (ftp://ftp.rcsb. org/pub/pdb/data/monomers/het_dictionary.txt ) for all ligands as well as standard residues and bases. Unrecognised ligand groups are flagged and any discrepancies in known ligands are ...
... Ligand and atom nomenclature. Residue and atom nomenclature is compared against the PDB dictionary (ftp://ftp.rcsb. org/pub/pdb/data/monomers/het_dictionary.txt ) for all ligands as well as standard residues and bases. Unrecognised ligand groups are flagged and any discrepancies in known ligands are ...
Gustatory Responses of Eel Palatine Receptors to Amino Acids and
... His) were effective at 10-4M, nine [isoleucine (Ile), leucine (Leu), phenylalanine (Phe), methionine (Met) tyrosine (Tyr), tryptophan (Trp), valine (Val), aspartic acid (Asp), glutamic acid (Glu)] did not elicit any response even at 10-2M. None of the three D-amino acids tested were as effective as ...
... His) were effective at 10-4M, nine [isoleucine (Ile), leucine (Leu), phenylalanine (Phe), methionine (Met) tyrosine (Tyr), tryptophan (Trp), valine (Val), aspartic acid (Asp), glutamic acid (Glu)] did not elicit any response even at 10-2M. None of the three D-amino acids tested were as effective as ...
Searching algorithm for type IV secretion system effectors 1.0: a tool
... L. pneumophila that are required for expression of T4SSencoding genes (42). Also, for other pathogenic bacteria, the expression of genes encoding secretion systems and those scattered in the genome encoding their substrates is co-regulated by one master regulatory protein that binds a consensus or i ...
... L. pneumophila that are required for expression of T4SSencoding genes (42). Also, for other pathogenic bacteria, the expression of genes encoding secretion systems and those scattered in the genome encoding their substrates is co-regulated by one master regulatory protein that binds a consensus or i ...
Protein kinases - Institut de recherches cliniques de Montréal
... How many phosphorylation sites are there? If there are ~10,000 proteins per cell with an average length of 400 aa (~ 17% of which are Ser, Thr or Tyr), then there are ~700,000 potential phosphorylation sites for any given kinase (including hidden residues). Although protein kinases have relatively ...
... How many phosphorylation sites are there? If there are ~10,000 proteins per cell with an average length of 400 aa (~ 17% of which are Ser, Thr or Tyr), then there are ~700,000 potential phosphorylation sites for any given kinase (including hidden residues). Although protein kinases have relatively ...
Interplay of pH and Binding of Multivalent Metal Ions: Charge
... quantitativelywith an effective short-ranged interaction between protein and ions, even for long-ranged and nonlocalized interactions.37 In this Article, we present findings on the charge inversion of globular proteins, in particular, bovine and human serum albumin (BSA, HSA), ovalbumin (OVA), and β- ...
... quantitativelywith an effective short-ranged interaction between protein and ions, even for long-ranged and nonlocalized interactions.37 In this Article, we present findings on the charge inversion of globular proteins, in particular, bovine and human serum albumin (BSA, HSA), ovalbumin (OVA), and β- ...
Recombinant human insulin-11. Size-exclusion HPLC of
... result of the fact, that the specimen molecules penetrate into sorbent pores, filled with the solvent, and are retained there for different time. Molecules, having a larger size in solution, either don't penetrate at all or penetrate only into part of the gel pores and are washed out of the column e ...
... result of the fact, that the specimen molecules penetrate into sorbent pores, filled with the solvent, and are retained there for different time. Molecules, having a larger size in solution, either don't penetrate at all or penetrate only into part of the gel pores and are washed out of the column e ...
figure 1 - Proceedings of the Royal Society B
... plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. Paralogs and alternatively spliced forms annotated in the same functional GO group were included in the dataset. The size ...
... plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. Paralogs and alternatively spliced forms annotated in the same functional GO group were included in the dataset. The size ...
Polypeptide Composition of Envelopes of Spinach Chloroplasts
... Preparation of intact chloroplasts—Spinach was purchased from a local farmer. Intact chloroplasts were prepared according to Siegenthaler and Dumont (1990) with some modifications. Leaves were homogenized for 10 s in the grinding medium containing 350 mM sorbitol, 25 mM 3-(/V-morpholino)propanesufon ...
... Preparation of intact chloroplasts—Spinach was purchased from a local farmer. Intact chloroplasts were prepared according to Siegenthaler and Dumont (1990) with some modifications. Leaves were homogenized for 10 s in the grinding medium containing 350 mM sorbitol, 25 mM 3-(/V-morpholino)propanesufon ...
from Scolopendra subspinipes mutilans
... histamine, lipids, polysaccharides and polypeptides, have been identified in crude extracts of centipede venom in previous studies [5, 6]. The venoms also play significant biologic roles in the synthesis of proteins, peptides and enzymes. In a recent study, centipede venoms that contained about 500 ...
... histamine, lipids, polysaccharides and polypeptides, have been identified in crude extracts of centipede venom in previous studies [5, 6]. The venoms also play significant biologic roles in the synthesis of proteins, peptides and enzymes. In a recent study, centipede venoms that contained about 500 ...
Signatures of nitrogen limitation in the elemental composition of the
... plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. Paralogs and alternatively spliced forms annotated in the same functional GO group were included in the dataset. The size ...
... plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. Paralogs and alternatively spliced forms annotated in the same functional GO group were included in the dataset. The size ...
Free tyrosine and tyrosine-rich peptide
... to be a portion of neurotoxic region), and VNITIKQHTVTTTT (helix 2, threonine-rich toxic sequence). The key common feature among the Cu-binding sequences in PrPs is the presence of His-residue(s) possibly behaving as the anchors for the copper [9,10]. The neurotoxic threonine-rich region correspondi ...
... to be a portion of neurotoxic region), and VNITIKQHTVTTTT (helix 2, threonine-rich toxic sequence). The key common feature among the Cu-binding sequences in PrPs is the presence of His-residue(s) possibly behaving as the anchors for the copper [9,10]. The neurotoxic threonine-rich region correspondi ...
Determination of Protein Molecular Weight
... Proteins exhibit different three-dimensional shapes and folding patterns which are determined by their amino acid sequences and intracellular processing. The precise three-dimensional configuration of a protein is critical to its function. The shapes these molecules can have are spherical, elliptica ...
... Proteins exhibit different three-dimensional shapes and folding patterns which are determined by their amino acid sequences and intracellular processing. The precise three-dimensional configuration of a protein is critical to its function. The shapes these molecules can have are spherical, elliptica ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... In addition to the results from precursor-accumulating vesicle studies (Hara-Nishimura et al., 1998), the results from studies of traffic of chimeric integral membrane proteins to vacuoles in tobacco suspension culture protoplasts indicated that some membrane components of organelles in the PSV path ...
... In addition to the results from precursor-accumulating vesicle studies (Hara-Nishimura et al., 1998), the results from studies of traffic of chimeric integral membrane proteins to vacuoles in tobacco suspension culture protoplasts indicated that some membrane components of organelles in the PSV path ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.