Chapter 3 Molecules of Life
... • Polysaccharides: chains of hundreds or thousands of monosaccharide monomers ...
... • Polysaccharides: chains of hundreds or thousands of monosaccharide monomers ...
IV.1 Structural and Functional Role of
... to a disruption of the interhelical packing due to the mutations. The fact that an increase in one or two carbon atoms makes such a significant difference indicates a tight steric coupling with another amino acid side chain. From the reported crystal structure [Palczewski et al., 2000], this interac ...
... to a disruption of the interhelical packing due to the mutations. The fact that an increase in one or two carbon atoms makes such a significant difference indicates a tight steric coupling with another amino acid side chain. From the reported crystal structure [Palczewski et al., 2000], this interac ...
Solubility-enhancing proteins MBP and NusA play a passive role in
... Two of the passenger proteins that exhibited at least moderate solubility after intracellular processing of the fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these ...
... Two of the passenger proteins that exhibited at least moderate solubility after intracellular processing of the fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these ...
Chapter 5 Gases - Saint Demetrios Astoria School
... • Polysaccharides: chains of hundreds or thousands of monosaccharide monomers ...
... • Polysaccharides: chains of hundreds or thousands of monosaccharide monomers ...
The Terminal Enzymes of Sialic Acid Metabolism: Acylneuraminate
... of special interest, because the nucleophilic attack of an active site residue on 4-OH is thought to represent an important step in catalysis (see below). The presence of a free carboxyl and a glycosidic hydroxyl group is absolutely indispensible. Already in the 1970s experiments were performed with ...
... of special interest, because the nucleophilic attack of an active site residue on 4-OH is thought to represent an important step in catalysis (see below). The presence of a free carboxyl and a glycosidic hydroxyl group is absolutely indispensible. Already in the 1970s experiments were performed with ...
Increasing the thermostability of sucrose
... found to be the most flexible region. This cluster is located in a loop segment of the C-terminal domain, far away from both the active site and dimer interface. The top 10 positions were then examined in the 3DM-database (Kuipers et al., 2010), which contains a structure-based alignment of all sequ ...
... found to be the most flexible region. This cluster is located in a loop segment of the C-terminal domain, far away from both the active site and dimer interface. The top 10 positions were then examined in the 3DM-database (Kuipers et al., 2010), which contains a structure-based alignment of all sequ ...
General and Physiological Chemistry
... Define and illustrate empirical formula, molecular formula, and structural formula. Based on the atomic numbers of the first 20 elements, predict if any given two of them can form a compound and if so predict whether the bonding in the compound is more likely to be ionic or covalent. For the A group ...
... Define and illustrate empirical formula, molecular formula, and structural formula. Based on the atomic numbers of the first 20 elements, predict if any given two of them can form a compound and if so predict whether the bonding in the compound is more likely to be ionic or covalent. For the A group ...
Relationship between Hot Spot Residues and Ligand Binding Hot
... of hot spots identified by alanine scanning mutagenesis versus by small molecule fragment screening, to clarify the relationship between the protein surface sites identified in these different ways, and to gain a better understanding of their implications for the discovery of small molecule inhibitors ...
... of hot spots identified by alanine scanning mutagenesis versus by small molecule fragment screening, to clarify the relationship between the protein surface sites identified in these different ways, and to gain a better understanding of their implications for the discovery of small molecule inhibitors ...
Expression of gene encoding immunodominant merozoite surface
... The duplicate wells of ELISA plate were coated separately with Tams1 protein at a concentration of 1,2 or 5 µg per mL of carbonate-bicarbonate buffer (pH 9.6) and titrated with two positive and two negative bovine sera diluted 1:50, 1:100 or 1:200 with PBS (pH 7.2) in standard ELISA format10. The co ...
... The duplicate wells of ELISA plate were coated separately with Tams1 protein at a concentration of 1,2 or 5 µg per mL of carbonate-bicarbonate buffer (pH 9.6) and titrated with two positive and two negative bovine sera diluted 1:50, 1:100 or 1:200 with PBS (pH 7.2) in standard ELISA format10. The co ...
GTPase domains ofras p21 oncogene protein and elongation factor
... biosynthesis, and membrane traffic. Amino acid sequences of more than 100 members of different subfamilies are known, but crystal structures of only mammalian ras p21 and bacterial elongation factor Tu have been determined. After optimal superposition of these remarkably similar structures, careful ...
... biosynthesis, and membrane traffic. Amino acid sequences of more than 100 members of different subfamilies are known, but crystal structures of only mammalian ras p21 and bacterial elongation factor Tu have been determined. After optimal superposition of these remarkably similar structures, careful ...
This is the accepted version of the following article:
... specific providing high yields and purity without the risk of structural or functional modification of isolated protein. Purifications based on affinity chromatography represent the most powerful tool available in term of the selectivity and recovery. Batch separation with specific antibodies as af ...
... specific providing high yields and purity without the risk of structural or functional modification of isolated protein. Purifications based on affinity chromatography represent the most powerful tool available in term of the selectivity and recovery. Batch separation with specific antibodies as af ...
MD Simulations of the P53 oncoprotein structure
... straight chain and is positively charged in neutral, acidic, and even most basic environments. Because of conjugation between the double bond and the nitrogen lone pairs, the positive charge is delocalized, enabling the formation of multiple H-bonds. Histidine (His) is an aromatic amino acid with pK ...
... straight chain and is positively charged in neutral, acidic, and even most basic environments. Because of conjugation between the double bond and the nitrogen lone pairs, the positive charge is delocalized, enabling the formation of multiple H-bonds. Histidine (His) is an aromatic amino acid with pK ...
Ribosome-tethered molecular chaperones
... from the cytosol by the ribosome exit tunnel [4]. Recent evidence indicates that after the chain leaves the tunnel, molecular chaperones bind, preventing aggregation. Both prokaryotic and eukaryotic chaperones have evolved to associate specifically with ribosomes and bind to polypeptide chains that ...
... from the cytosol by the ribosome exit tunnel [4]. Recent evidence indicates that after the chain leaves the tunnel, molecular chaperones bind, preventing aggregation. Both prokaryotic and eukaryotic chaperones have evolved to associate specifically with ribosomes and bind to polypeptide chains that ...
MB ChB PHASE I
... Of the 20 coded amino-acids, 10 (‘non-essential’) can be synthesised in the body (they have simple R groups); 8 (‘essential’) cannot be synthesised and must be present in the diet; 2 (arginine, histidine) can usually be made at adequate rates, but are needed in the diet when tissue growth is rapid. ...
... Of the 20 coded amino-acids, 10 (‘non-essential’) can be synthesised in the body (they have simple R groups); 8 (‘essential’) cannot be synthesised and must be present in the diet; 2 (arginine, histidine) can usually be made at adequate rates, but are needed in the diet when tissue growth is rapid. ...
Chapter 10 - People Server at UNCW
... Protein Structure • Proteins fold into one or more 3-D shapes or conformations • Based on attraction and repulsion between atoms of proteins, and interactions of proteins with chemicals in the environment ...
... Protein Structure • Proteins fold into one or more 3-D shapes or conformations • Based on attraction and repulsion between atoms of proteins, and interactions of proteins with chemicals in the environment ...
Gene Section JUND (proto-oncogene) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... JUND is a member of the JUN family of basic region leucine zipper (bZIP) DNA-binding proteins. Analysis of the protein expression levels demonstrated an opposite expression pattern between JUN and JUND. When cells entry into the G0 phase of the cell cycle by serum starvation, JUN level decreases and ...
... JUND is a member of the JUN family of basic region leucine zipper (bZIP) DNA-binding proteins. Analysis of the protein expression levels demonstrated an opposite expression pattern between JUN and JUND. When cells entry into the G0 phase of the cell cycle by serum starvation, JUN level decreases and ...
The Organic Chemistry of Amino Acids, Peptides, and Proteins
... The next step is to determine the number and kinds of amino acids in the peptide or protein by hydrolysis of all the amide bonds and then analysis of the mixture. ...
... The next step is to determine the number and kinds of amino acids in the peptide or protein by hydrolysis of all the amide bonds and then analysis of the mixture. ...
Variations in amino acid composition in bacterial single stranded
... known as OB-fold (a structurally conserved folding motif) (8). The OB-fold binds to ssDNA through a combination of electrostatic and base-stacking interactions with the phosphodiester backbone and nucleotide bases (9). It comprises five antiparallel b-sheets which form a b-barrel capped by an a-heli ...
... known as OB-fold (a structurally conserved folding motif) (8). The OB-fold binds to ssDNA through a combination of electrostatic and base-stacking interactions with the phosphodiester backbone and nucleotide bases (9). It comprises five antiparallel b-sheets which form a b-barrel capped by an a-heli ...
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... secondary conformational transition from structurally unorganized monomers into metastable R-helical intermediates that terminate in cross-β structures. The peptides have a characteristic sequence motif that consists of an aliphatic amino acid tail of decreasing hydrophobicity capped by a polar head ...
... secondary conformational transition from structurally unorganized monomers into metastable R-helical intermediates that terminate in cross-β structures. The peptides have a characteristic sequence motif that consists of an aliphatic amino acid tail of decreasing hydrophobicity capped by a polar head ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.