Response of Jujube Fruits to Exogenous Oxalic Acid Treatment
... induce the programmed cell death response in plant tissue not by reducing the pH value but by inducing increased reactive oxygen species (ROS) levels in the plant. Meanwhile, some evidence showed that OA has a potential effect on inducing resistance against pathogen and antioxidant systems. Kayashim ...
... induce the programmed cell death response in plant tissue not by reducing the pH value but by inducing increased reactive oxygen species (ROS) levels in the plant. Meanwhile, some evidence showed that OA has a potential effect on inducing resistance against pathogen and antioxidant systems. Kayashim ...
Mutational analysis of exoribonuclease I from Saccharomyces
... screened by western blotting for expression of Xrn1p containing the HA epitope located in the C-terminus of the protein. This yielded 10 missense mutations in region I, six in region II, one in region III and one in region IV. Region I and II mutants containing multiple mutations were sub-cloned to ...
... screened by western blotting for expression of Xrn1p containing the HA epitope located in the C-terminus of the protein. This yielded 10 missense mutations in region I, six in region II, one in region III and one in region IV. Region I and II mutants containing multiple mutations were sub-cloned to ...
Serine/Threonine Phosphatases
... phosphatase. In contrast to PPP, members of the PPM family do not have regulatory subunits but contain instead additional domains and conserved sequence motifs that may help determine substrate specificity. For both PPP and PPM, metal ions play a catalytic and central role through the activation of ...
... phosphatase. In contrast to PPP, members of the PPM family do not have regulatory subunits but contain instead additional domains and conserved sequence motifs that may help determine substrate specificity. For both PPP and PPM, metal ions play a catalytic and central role through the activation of ...
Marine alga Sargassum horneri active component
... imported glutamine is then exported by the bidirectional amino acid transporter SLC7A5, which transports the extracellular essential amino acids into cells [11,44]. This indicates that extracellular amino acid signaling is not the same as intracellular amino acid signaling. Therefore, it is difficul ...
... imported glutamine is then exported by the bidirectional amino acid transporter SLC7A5, which transports the extracellular essential amino acids into cells [11,44]. This indicates that extracellular amino acid signaling is not the same as intracellular amino acid signaling. Therefore, it is difficul ...
the scf ubiquitin ligase: insights into a molecular machine
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
Effects of rare codon clusters on high-level expression
... one major and two minor molecular weight species. The major truncated species coincided with a +1 frameshift occurring at the second codon o f an AGG tandem located at positions 208-209. Co-expression o f the argU gene completely eliminated this prematurely terminated species, but did not affect the ...
... one major and two minor molecular weight species. The major truncated species coincided with a +1 frameshift occurring at the second codon o f an AGG tandem located at positions 208-209. Co-expression o f the argU gene completely eliminated this prematurely terminated species, but did not affect the ...
A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN
... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
Transport of Aromatic Amino Acids by Brevibacterium linens
... than 38% (Table 1). It would appear that the aamino and a-carboxyl are less important in this case than for the transport of the other two aromatic amino acids, since these functional groups were absent from potent inhibitors. The length of the carbon chain on the indole ring also seems to be of sec ...
... than 38% (Table 1). It would appear that the aamino and a-carboxyl are less important in this case than for the transport of the other two aromatic amino acids, since these functional groups were absent from potent inhibitors. The length of the carbon chain on the indole ring also seems to be of sec ...
Role of Dietary Protein in Post-Exercise Muscle Reconditioning
... that is rapidly digested and absorbed following ingestion. In contrast, intact casein tends to clot in the stomach after ingestion, thereby delaying digestion and absorption, resulting in a slower release of protein-derived amino acids in the circulation [25]. The faster, more transient rise in plas ...
... that is rapidly digested and absorbed following ingestion. In contrast, intact casein tends to clot in the stomach after ingestion, thereby delaying digestion and absorption, resulting in a slower release of protein-derived amino acids in the circulation [25]. The faster, more transient rise in plas ...
The hepatitis C virus Core protein is a potent nucleic acid chaperone
... can be assayed by examining the strand exchange activity of the protein (see Fig. 3A). First, an imperfect DNA duplex with two partially complementary ODNs was formed by heating. The Core protein was then added together with another ODN with the potential to form a perfect duplex with one of the two ...
... can be assayed by examining the strand exchange activity of the protein (see Fig. 3A). First, an imperfect DNA duplex with two partially complementary ODNs was formed by heating. The Core protein was then added together with another ODN with the potential to form a perfect duplex with one of the two ...
The Origin of Life on Earth by Leslie E. Orgel Growing evidence
... life itself - would have been abundant on the primitive planet. At the time, investigators were not yet paying much attention to the origin of nucleic acids- they were most interested in explaining how proteins appeared on the earth. Careful analyses elucidated many of the chemical reactions that oc ...
... life itself - would have been abundant on the primitive planet. At the time, investigators were not yet paying much attention to the origin of nucleic acids- they were most interested in explaining how proteins appeared on the earth. Careful analyses elucidated many of the chemical reactions that oc ...
Universal strategies in research and drug discovery based on protein
... represented on the left, and the interactions between the pathway enzymes are depicted on the right. Perturbation of one enzyme (B4) with, for example, a drug, changes the interactions of other enzymes coupled to B4 (B6:B7). This way it can be inferred that these enzymes have a role in the same bioc ...
... represented on the left, and the interactions between the pathway enzymes are depicted on the right. Perturbation of one enzyme (B4) with, for example, a drug, changes the interactions of other enzymes coupled to B4 (B6:B7). This way it can be inferred that these enzymes have a role in the same bioc ...
Proteases and proteolytic cleavage of storage
... This is a heterogeneous group of proteins where polypeptides predominate which are structurally related to the napin-like 2S proteins from Cruciferae (Shewry and Tatham, 1990). 2S storage proteins are polymorphic and encoded by multigene families. The proteins are monomeric and consist of disulphide ...
... This is a heterogeneous group of proteins where polypeptides predominate which are structurally related to the napin-like 2S proteins from Cruciferae (Shewry and Tatham, 1990). 2S storage proteins are polymorphic and encoded by multigene families. The proteins are monomeric and consist of disulphide ...
SpyTag and SpyCatcher - Department of Biochemistry
... [22,23]. We generated SpyTag and SpyCatcher by splitting the CnaB2 domain from the fibronectin-binding protein FbaB from Streptococcus pyogenes (Spy) [24,25]. CnaB2 was split into a 13 residue peptide (SpyTag) and the 116 residue complementary domain (SpyCatcher) (Figure 1a). These two parts spontan ...
... [22,23]. We generated SpyTag and SpyCatcher by splitting the CnaB2 domain from the fibronectin-binding protein FbaB from Streptococcus pyogenes (Spy) [24,25]. CnaB2 was split into a 13 residue peptide (SpyTag) and the 116 residue complementary domain (SpyCatcher) (Figure 1a). These two parts spontan ...
Identification of a novel N-terminal hydrophobic sequence that
... is the proline knot, a ~72 amino acid hydrophobic sequence found in the middle of plant oleosins. The proline knot is thought to direct the protein to the droplet from its site of insertion in the ER, leaving flanking hydrophilic residues in the cytoplasm. The flanking sequences may also participate ...
... is the proline knot, a ~72 amino acid hydrophobic sequence found in the middle of plant oleosins. The proline knot is thought to direct the protein to the droplet from its site of insertion in the ER, leaving flanking hydrophilic residues in the cytoplasm. The flanking sequences may also participate ...
Animal Dicer and plant Dicer-like proteins
... precursors is a crucial step, common for most srRNA biogenesis pathways. A group of enzymes belonging to the RNase III family have been identified as the key players involved in this process (Bernstein et al., 2001). In animals these enzymes are called Dicer proteins, while in plants they are known ...
... precursors is a crucial step, common for most srRNA biogenesis pathways. A group of enzymes belonging to the RNase III family have been identified as the key players involved in this process (Bernstein et al., 2001). In animals these enzymes are called Dicer proteins, while in plants they are known ...
The Three-dimensional Structure of Transfer RNA
... on one strand of the helix is always paired with the base cytosine on the oth er strand. and the base adenine is always paired with the base thymine. Immediately adjacent to the A site in the ribosome is the peptidyl-tRNA bind ing site. or P site. The transfer-RNA molecule with the growing chain o ...
... on one strand of the helix is always paired with the base cytosine on the oth er strand. and the base adenine is always paired with the base thymine. Immediately adjacent to the A site in the ribosome is the peptidyl-tRNA bind ing site. or P site. The transfer-RNA molecule with the growing chain o ...
RELIC – A bioinformatics server for combinatorial
... of the small molecule binding motifs and is highly predictive for binding of nucleotide triphosphates, many ATPbinding sites do not in fact contain a p-loop motif. Threedimensional information from crystallographic structures provides a strong basis for prediction of ligand binding which, when combi ...
... of the small molecule binding motifs and is highly predictive for binding of nucleotide triphosphates, many ATPbinding sites do not in fact contain a p-loop motif. Threedimensional information from crystallographic structures provides a strong basis for prediction of ligand binding which, when combi ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.