Biochemical Journal
... catalytic domain of LiCMS) in complex with Mal (malonate) at 2.0 Å (1 Å = 0.1 nm) resolution, in complex with Pyr at 2.6 Å resolution and in complex with Pyr and acetyl-CoA at 2.5 Å resolution. Analyses of these structures, together with mutagenesis and kinetic studies, identified the key residues i ...
... catalytic domain of LiCMS) in complex with Mal (malonate) at 2.0 Å (1 Å = 0.1 nm) resolution, in complex with Pyr at 2.6 Å resolution and in complex with Pyr and acetyl-CoA at 2.5 Å resolution. Analyses of these structures, together with mutagenesis and kinetic studies, identified the key residues i ...
UNIVERSITAT DE BARCELONA Divisió de Ciències Experimentals i Matemàtiques Facultat de Biologia
... Since the oxidation of -SH groups was higher than expected, and the recoveries of cysteine and methionine poor, these data, together with those of tryptophan —destroyed in the acid hydrolysis procedure (Hill, 1965)— were not included in the tabulated results. References to "total" amino acids do not ...
... Since the oxidation of -SH groups was higher than expected, and the recoveries of cysteine and methionine poor, these data, together with those of tryptophan —destroyed in the acid hydrolysis procedure (Hill, 1965)— were not included in the tabulated results. References to "total" amino acids do not ...
DOCTORAL THESIS
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent
... from E. gracilis (143.170 kb) [15,16]. The sets of genes encoding proteins involved in transcription and translation are nearly identical, except for the rps18 gene missing from E. longa. In both species the genome harbors a region comprising three tandemly arrayed operons, each including the 16S, 2 ...
... from E. gracilis (143.170 kb) [15,16]. The sets of genes encoding proteins involved in transcription and translation are nearly identical, except for the rps18 gene missing from E. longa. In both species the genome harbors a region comprising three tandemly arrayed operons, each including the 16S, 2 ...
Lactococcus lactis as expression host for the biosynthetic
... inducible expression of proteins that contain the peptidoglycanbinding domain of the lactococcal cell-wall-hydrolysing enzyme AcmA [16,17]. The entire peptidoglycan-binding domain is called PA90 and contains five Trp residues. PA6 is part of PA90 and contains one Trp residue. In the plasmids contain ...
... inducible expression of proteins that contain the peptidoglycanbinding domain of the lactococcal cell-wall-hydrolysing enzyme AcmA [16,17]. The entire peptidoglycan-binding domain is called PA90 and contains five Trp residues. PA6 is part of PA90 and contains one Trp residue. In the plasmids contain ...
HEMOSTASIS
... A number of GPs present in this area are responsible for blood group specificity (ABO), tissue compatibility (HLA), and platelet antigenicity. Platelet membrane glyco-proteins serve as a receptor and facilitate transmission of stimuli across the platelet membrane. Platelet membrane GPIb appears to b ...
... A number of GPs present in this area are responsible for blood group specificity (ABO), tissue compatibility (HLA), and platelet antigenicity. Platelet membrane glyco-proteins serve as a receptor and facilitate transmission of stimuli across the platelet membrane. Platelet membrane GPIb appears to b ...
as a PDF
... respiration-permissive) conditions as described previously (12). Note that metabolism is fermentative under both static and aerated conditions. Bidimensional electrophoresis. (i) Sample preparation. Cells were harvested in late exponential growth phase at an optical density at 600 nm (OD600) of 1.6 ...
... respiration-permissive) conditions as described previously (12). Note that metabolism is fermentative under both static and aerated conditions. Bidimensional electrophoresis. (i) Sample preparation. Cells were harvested in late exponential growth phase at an optical density at 600 nm (OD600) of 1.6 ...
Identification and characterization of novel interaction partners of
... Institut für Genetik, Heinrich-Heine Universität Düsseldorf, Germany ...
... Institut für Genetik, Heinrich-Heine Universität Düsseldorf, Germany ...
Evidence from glycine transfer RNA of a frozen accident at the dawn
... A37 has been replaced by G, perhaps because of a general increase in G+C content in the tRNA and rRNA of these species [18]. There are three other sequences not containing an anticodon loop CCA that appear to be isolated examples, but in these cases their origin is unclear. Schimmel's experimental w ...
... A37 has been replaced by G, perhaps because of a general increase in G+C content in the tRNA and rRNA of these species [18]. There are three other sequences not containing an anticodon loop CCA that appear to be isolated examples, but in these cases their origin is unclear. Schimmel's experimental w ...
Introduction to Bioinformatics
... • Hydrophilic amino acids are water soluable • Hydrophobic are not • Linked via a single chemical bond (peptide bond) • Peptide: Short linear chain of amino acids (< 30) polypeptide: long chain of amino acids (which can be upwards of 4000 residues long). ...
... • Hydrophilic amino acids are water soluable • Hydrophobic are not • Linked via a single chemical bond (peptide bond) • Peptide: Short linear chain of amino acids (< 30) polypeptide: long chain of amino acids (which can be upwards of 4000 residues long). ...
cerevisiae - Oxford Academic
... what we know about the residues inserted by readthrough at stop codons in eukaryotes or about the natural suppressor tRNAs involved in this process was gleaned from in vitro experiments in rabbit reticulocyte lysate (RRL) (20). For example, tryptophan, cysteine and arginine were identified at UGA st ...
... what we know about the residues inserted by readthrough at stop codons in eukaryotes or about the natural suppressor tRNAs involved in this process was gleaned from in vitro experiments in rabbit reticulocyte lysate (RRL) (20). For example, tryptophan, cysteine and arginine were identified at UGA st ...
Lecture_6-2
... • The cells in a log odds matrix consist of an “odds ratio”: the probability that an alignment is authentic the probability that the alignment was random The score S for an alignment of residues a,b is given by: S(a,b) = 10 log10 ( Mab / pb ) As an example, for tryptophan, S( W, W ) = 10 log10 ( 0.5 ...
... • The cells in a log odds matrix consist of an “odds ratio”: the probability that an alignment is authentic the probability that the alignment was random The score S for an alignment of residues a,b is given by: S(a,b) = 10 log10 ( Mab / pb ) As an example, for tryptophan, S( W, W ) = 10 log10 ( 0.5 ...
Shigella boydii Involved in O-antigen Synthesis By
... 2.1.1 Construction of the mutants .......................................................................- 24 2.1.2 Transformation of plasmid constructs into the expression cell lines ............- 26 2.1.3 Expression of wild type and mutant wfeD in E.coli BL21 cells ..................- 27 2.2 Gala ...
... 2.1.1 Construction of the mutants .......................................................................- 24 2.1.2 Transformation of plasmid constructs into the expression cell lines ............- 26 2.1.3 Expression of wild type and mutant wfeD in E.coli BL21 cells ..................- 27 2.2 Gala ...
Effects of low crude-protein diets fortified with crystalline amino acids
... potential reasons for the relatively poor performance of broiler chicks fed low crude protein (CP) diets. Male chicks ( l-d-old) were fed a common corn-soybean (SBM) diet (23% CP) for 7 d and subsequently allotted to treatment diets in a completely randomized design (10 chicks per floor pen, 6 repli ...
... potential reasons for the relatively poor performance of broiler chicks fed low crude protein (CP) diets. Male chicks ( l-d-old) were fed a common corn-soybean (SBM) diet (23% CP) for 7 d and subsequently allotted to treatment diets in a completely randomized design (10 chicks per floor pen, 6 repli ...
Further studies on the new coomassie brilliant blue G-250 - K-REx
... Consequently, it was decided to investigate the potential of this dye more fully than it had been dealt with in Bradford's report. ...
... Consequently, it was decided to investigate the potential of this dye more fully than it had been dealt with in Bradford's report. ...
Staphylococcus haemolyticus lipase
... zyme forms, which have molecular masses of approximately 80 kDa. After secretion into the growth medium, proteolytic processing results in mature forms with molecular masses of 40^46 kDa [8]. Many comparative studies of S. aureus lipase (SAL) and SHL have been done and in spite of high homology (50^ ...
... zyme forms, which have molecular masses of approximately 80 kDa. After secretion into the growth medium, proteolytic processing results in mature forms with molecular masses of 40^46 kDa [8]. Many comparative studies of S. aureus lipase (SAL) and SHL have been done and in spite of high homology (50^ ...
Lipid Rafts Presentation
... is found in the brain…CNS? When removed, most proteins dissociate from rafts ...
... is found in the brain…CNS? When removed, most proteins dissociate from rafts ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.