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Molecular Biology Fourth Edition
Molecular Biology Fourth Edition

... Major and minor grooves  The major groove is large enough to accommodate an alpha helix from a protein.  Regulatory proteins (transcription factors) can recognize the pattern of bases and H-bonding possibilities in the major groove. ...
Supplemental Data
Supplemental Data

... Gal80p-Gal80p interaction. Gal80p-Gal80p interactions were analyzed by complex formation with a Gal80p derivative with altered gel mobility. A fusion between Gal80p and the negatively charged activation domain of Herpes simplex VP16 (Gal80pVP16) migrates faster on native gels than Gal80p by itself. ...
A Comparison of Suffix Tree based Indexing and Search
A Comparison of Suffix Tree based Indexing and Search

... represent 3-D substructures as compared to sequence of strings in the other two approaches.In this section we are going to see how the features are extracted and data is prepared for construction of suffix trees. From the chain of carbon atoms PSIST uses a sliding window to extract local feature vec ...
lecture notes-enzyme-web
lecture notes-enzyme-web

... - Lyases:catalyze the breaking of various chemical bonds by means other than hydrolysis and oxidation, often forming a new double bond or a new ring structure e.g. CH3COCO-OH → CH3COCHO (dehydratase) - Isomerases:catalyse the interconversion of isomers. e.g.phosphoglucose isomerase that converts glu ...
1 • Introduction The Scientific Method (1 of 20) 1
1 • Introduction The Scientific Method (1 of 20) 1

... observations. “This beaker contains water” is an example. You infer (probably correctly) it is water because it is a clear, colorless liquid that came from the tap. The observations are that it is clear, it is colorless, it is a liquid, and it came from the tap. ...
Higher Chemistry summary 3a
Higher Chemistry summary 3a

... annum suitable for fast single step processes more easily automated using computer control smaller work force operates round the clock, 365 days per year tend to operate with relatively low volumes of reactants allowing easy removal of excess heat energy ...
The random character of protein evolution and its effect on the
The random character of protein evolution and its effect on the

... (up to 400 per 100 residues). The other 'tree' was a more complex one with seven descendants, with topology and times of separation based on the evolution of seven hoofed mammals (sheep, goat, cow, llama, pig, horse and donkey) as given by Langley & Fitch (1974). It is shown as an inset in Fig. 3. T ...
Enzyme Mechanisms: Serine Proteases Questions
Enzyme Mechanisms: Serine Proteases Questions

... 1. The substrate specificity of serine proteases is primarily due to  A) a specificity pocket in the protein.  B) the positions of specific side chains of serine, histidine, and aspartate. C) distinct backbone conformations of the individual proteins. D) A and B.  E) A, B and C.  2. The role of seri ...
LIMS for the Masses
LIMS for the Masses

... (nutritional, xenobiotic, surgical or toxic stimuli) • Metabolomics:The quantitative measurement of the metabolic profiles of model organisms to characterize their phenotype or phenotypic response to genetic or nutritional perturbations ...
Complete the following equations
Complete the following equations

... The Oswald process for the production of nitric acid involves the following reactions: (i) 4NH3(g) + 5 O2(g)  4 NO(g) + 6H2O(g); (ii) 2 NO(g) + O2(g)  2 NO2(g); (iii) 3 NO2(g) + H2O(l)  2 HNO3(l) + NO(g); (a) Calculate the enthalpy change (H, in kJ) for each reaction. (b) Balance the following e ...
Are phloem amino acids involved in the shoot to root control of NO
Are phloem amino acids involved in the shoot to root control of NO

... N-deficiency is controlled by shoot-borne regulatory signals, and provide a case study where these signals are not related to a qualitative change or a significant decrease in downward phloem transport of amino acids. Key words: N-deficiency, NO− uptake, phloem amino ...
Chapter 2 - My Teacher Site
Chapter 2 - My Teacher Site

... The transfer of an electron is not the formation of a bond itself; rather it simply allows an ionic bond to form ...
No Slide Title
No Slide Title

... equation for the reaction. Although this equation shows the reactants and products of the reaction, it does not give a very clear picture of what truly occurs in solution. In fact, such an aqueous solution actually contains individual IONS, not molecules, in solution. By looking at the aforementione ...
CHAP NUM="8" ID="CH
CHAP NUM="8" ID="CH

Biologically Assembled Nanobiocatalysts Heejae Kim Qing Sun
Biologically Assembled Nanobiocatalysts Heejae Kim Qing Sun

... Leucine zippers are helical peptides that dimerized based on hydrophobic coiled-coil interactions. Each a-helix contains multiple leucine residues at *7-residue intervals, creating a hydrophobic region on one side of the coil for dimerization between two complementary leucine zippers. Because of the ...
Review Session One
Review Session One

... 1. What is a hydrogen bond? --- VERY important! ...
Moonlighting proteins—an update
Moonlighting proteins—an update

... of factors might be employed. Of the new examples, some proteins make use of the general methods seen in previously identified moonlighting proteins to switch between functions, although the details differ, for example how interacting with a different protein partner or cofactor results in a conformati ...
Prediction of mitochondrial proteins of malaria parasite
Prediction of mitochondrial proteins of malaria parasite

... acid composition. We developed a SVM model using amino acid composition and got maximum MCC 0.38 with accuracy of 74.14%. Similarly, SVM models were developed using dipeptide composition which achieved maximum MCC 0.50 with accuracy of 82.76%. In past it has been shown that composition of parts of a ...
Bacterial Bioreactors for High Yield Production of Recombinant Protein
Bacterial Bioreactors for High Yield Production of Recombinant Protein

... substantial (40-fold) condensation without a reduction in the yield of target protein. Condensed Culture Conditions Support High Efficiency Selenomethionine Incorporation without Toxicity—The ability to produce high levels of a single protein in condensed cultures may have applications for structura ...
Full contents - Scion Publishing
Full contents - Scion Publishing

... Box 6.1 Representations of monosaccharide structures........................ 00 Box 6.2 The different types of isomers relevant to carbohydrate ...
Page 20-1 CHAPTER 20: Enzymes 20.2
Page 20-1 CHAPTER 20: Enzymes 20.2

... Lactate Dehydrogenase ...
الشريحة 1
الشريحة 1

... suspended in a gel. • The microscopic particles attach to one another forming tunnels that act as a sieve to separate the molecules. • Small molecules can move faster than large molecules. ...
Protein Folding Cell and Mol Biology Lab
Protein Folding Cell and Mol Biology Lab

... and Proline 202 in carbonic anhydrase are cis prolines and you want to see where they are in the structure. In the "Find" dialog box, ask it to look for the two strings "pro" and "202" ("find" is not sensitive to upper vs lower case). It will put a marker on the point, or center it if you turn on pi ...
Mutational properties of amino acid residues: implications for
Mutational properties of amino acid residues: implications for

... Figure 1. Creative methodologies and evolution. As an analogy to protein evolution in the hunt for new protein function, we have illustrated different strategies to design a radio. (a) As Jacob described several years ago, nature does not evolve by creating de novo protein function from a blank canv ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... (4) Which one of the following enzymes is used in detergents? (a) Pectinase (b) Xylanase (c) Laccase (d) Protease (5) Diagnostic enzymes that are released into the serum due to organ damage are known as (a) Primary enzymes (b) Secondary enzymes (c) Isozymes (d) Tertiary enzymes II. State whether the ...
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Biochemistry



Biochemistry, sometimes called biological chemistry, is the study of chemical processes within and relating to living organisms. By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism, biochemical processes give rise to the complexity of life. Over the last decades of the 20th century, biochemistry has become so successful at explaining living processes that now almost all areas of the life sciences from botany to medicine to genetics are engaged in biochemical research. Today, the main focus of pure biochemistry is in understanding how biological molecules give rise to the processes that occur within living cells, which in turn relates greatly to the study and understanding of whole organisms.Biochemistry is closely related to molecular biology, the study of the molecular mechanisms by which genetic information encoded in DNA is able to result in the processes of life. Depending on the exact definition of the terms used, molecular biology can be thought of as a branch of biochemistry, or biochemistry as a tool with which to investigate and study molecular biology.Much of biochemistry deals with the structures, functions and interactions of biological macromolecules, such as proteins, nucleic acids, carbohydrates and lipids, which provide the structure of cells and perform many of the functions associated with life. The chemistry of the cell also depends on the reactions of smaller molecules and ions. These can be inorganic, for example water and metal ions, or organic, for example the amino acids which are used to synthesize proteins. The mechanisms by which cells harness energy from their environment via chemical reactions are known as metabolism. The findings of biochemistry are applied primarily in medicine, nutrition, and agriculture. In medicine, biochemists investigate the causes and cures of disease. In nutrition, they study how to maintain health and study the effects of nutritional deficiencies. In agriculture, biochemists investigate soil and fertilizers, and try to discover ways to improve crop cultivation, crop storage and pest control.
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