Kinetics of Enzyme-Catalyzed Reactions
... Since noncompetitive inhibitors do not interfere in the equilibration of enzyme, substrate and ES complexes, the Km's of Michaelis-Menten type enzymes are not expected to be affected by noncompetitive inhibitors, as demonstrated by x-axis intercepts in panel C. However, because complexes that conta ...
... Since noncompetitive inhibitors do not interfere in the equilibration of enzyme, substrate and ES complexes, the Km's of Michaelis-Menten type enzymes are not expected to be affected by noncompetitive inhibitors, as demonstrated by x-axis intercepts in panel C. However, because complexes that conta ...
Mammalian Cell Culture: High Throughput Applications of
... 3 INTRODUCTION 3.1 Objective The history of cell culture dates back to early twentieth century. However, it was only during the 1940’s and 1950’s that there was a rapid development in the techniques for cell culture. Mammalian cells are cells which are generally part of an organ of an organism, diff ...
... 3 INTRODUCTION 3.1 Objective The history of cell culture dates back to early twentieth century. However, it was only during the 1940’s and 1950’s that there was a rapid development in the techniques for cell culture. Mammalian cells are cells which are generally part of an organ of an organism, diff ...
Liver Function in Type-2 Diabetes Mellitus Patients
... These findings are consistent with the results obtained from several other studies by various researchers. According to Gonem et al., it was identified that the prevalence of ALT enzyme activity in diabetic patients (n = 959) was 15.7% (151).18 ALT catalyzes the reversible transamination between L-a ...
... These findings are consistent with the results obtained from several other studies by various researchers. According to Gonem et al., it was identified that the prevalence of ALT enzyme activity in diabetic patients (n = 959) was 15.7% (151).18 ALT catalyzes the reversible transamination between L-a ...
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... In this lesson students will demonstrate the process of aerobic and anaerobic cellular respiration and identify where it occurs The Main Man - Mitochondria within the cell. Students will review with a video, label a mitochondria, and create a eukaryotic cell tracing the path of cellular respiration. ...
... In this lesson students will demonstrate the process of aerobic and anaerobic cellular respiration and identify where it occurs The Main Man - Mitochondria within the cell. Students will review with a video, label a mitochondria, and create a eukaryotic cell tracing the path of cellular respiration. ...
Identification of a family of BspA like surface proteins of Entamoeba
... TpLRRP repeats, but possess only 5 TpLRRP repeats resulting in a significantly smaller protein, with a different C-terminus sequence (Fig. 1B). The EhLRRP1 protein appears to contain 22 TpLRR-like repeats of which 12 show near perfect identity to the consensus sequence (LxxIxIxxxVxxIgxxAFxxCxx), whi ...
... TpLRRP repeats, but possess only 5 TpLRRP repeats resulting in a significantly smaller protein, with a different C-terminus sequence (Fig. 1B). The EhLRRP1 protein appears to contain 22 TpLRR-like repeats of which 12 show near perfect identity to the consensus sequence (LxxIxIxxxVxxIgxxAFxxCxx), whi ...
Knocking Down of Isoprene Emission Modiies the
... Isoprene is a small lipophilic molecule with important functions in plant protection against abiotic stresses. Here, we studied the lipid composition of thylakoid membranes and chloroplast ultrastructure in isoprene-emitting (IE) and nonisoprene-emitting (NE) poplar (Populus 3 canescens). We demonst ...
... Isoprene is a small lipophilic molecule with important functions in plant protection against abiotic stresses. Here, we studied the lipid composition of thylakoid membranes and chloroplast ultrastructure in isoprene-emitting (IE) and nonisoprene-emitting (NE) poplar (Populus 3 canescens). We demonst ...
Engineering carbonic anhydrase for highly selective ester hydrolysis Gunnar Höst
... unfolded polypeptide chain and sufficiently stable once formed. This places restrictions on the possible sequences, so that only a small subset of the protein sequence space is actually found in nature. The structural organization of a protein is usually described as consisting of four different lev ...
... unfolded polypeptide chain and sufficiently stable once formed. This places restrictions on the possible sequences, so that only a small subset of the protein sequence space is actually found in nature. The structural organization of a protein is usually described as consisting of four different lev ...
Adaptative biochemical pathways and regulatory networks in
... on FEC is coupled with iron-binding EPS production [12, 16] at an average yield of 6.7 g/L (dry weight EPS per culture volume). Proteomic 2D-DIGE maps showed the occurrence of 869 reproducible protein spots (i. e. detected in all 2D-Gels) which were used to create the match set for comparative analy ...
... on FEC is coupled with iron-binding EPS production [12, 16] at an average yield of 6.7 g/L (dry weight EPS per culture volume). Proteomic 2D-DIGE maps showed the occurrence of 869 reproducible protein spots (i. e. detected in all 2D-Gels) which were used to create the match set for comparative analy ...
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... primary structure of proteins. Secondary structure of proteins: The secondary structure of proteins refers to the shape in which a long polypeptide can exist. The two different secondary structure possible are α -Helix structure and β – pleated sheet structure. • α -Helix structure: In α -Helix stru ...
... primary structure of proteins. Secondary structure of proteins: The secondary structure of proteins refers to the shape in which a long polypeptide can exist. The two different secondary structure possible are α -Helix structure and β – pleated sheet structure. • α -Helix structure: In α -Helix stru ...
Whole body and tissue protein synthesis in cattle
... In all three animals the rate of rise in the specific radioactivity of the blood free tyrosine and leucine was rapid ; the lowest rate-constant observed for tyrosine was 35/d for heifer no. 439 (Fig. I). The variation in specific radioactivity of blood free tyrosine at plateau (3-8 h) was greatest i ...
... In all three animals the rate of rise in the specific radioactivity of the blood free tyrosine and leucine was rapid ; the lowest rate-constant observed for tyrosine was 35/d for heifer no. 439 (Fig. I). The variation in specific radioactivity of blood free tyrosine at plateau (3-8 h) was greatest i ...
University of Groningen Sugar transport in
... or carbohydrate uptake transporter (CUT)-family, and the di/oligopeptide transport-, or Opp-family [22]. These two families differ not only in substrate specificity but also in the architecture of the transport complex. Members of the bacterial CUT-family are involved in the uptake of glycerol-phosp ...
... or carbohydrate uptake transporter (CUT)-family, and the di/oligopeptide transport-, or Opp-family [22]. These two families differ not only in substrate specificity but also in the architecture of the transport complex. Members of the bacterial CUT-family are involved in the uptake of glycerol-phosp ...
Pyruvate and Acetate Metabolism in the Photosynthetic Bacterium
... capsulatus cells grown photosynthetically with different carbon sources. Pyruvate dehydrogenase, pyruvate carboxylase, pyruvate kinase and phosphoenolpyruvate carboxykinase (ATP) were all present in extracts of cells grown on DL-lactate, whereas phosphoenolpyruvate carboxylase, phosphoenolpyruvate s ...
... capsulatus cells grown photosynthetically with different carbon sources. Pyruvate dehydrogenase, pyruvate carboxylase, pyruvate kinase and phosphoenolpyruvate carboxykinase (ATP) were all present in extracts of cells grown on DL-lactate, whereas phosphoenolpyruvate carboxylase, phosphoenolpyruvate s ...
13 Aldehydes and Ketones
... Could RNA have evolved from simple molecules in the “primordial soup”? In the 1960s two of the components of RNA, adenine and guanine, were synthesized in the laboratory from simple molecules and energy sources thought to be present on early earth. In 1995 researchers discovered that, by adding the ...
... Could RNA have evolved from simple molecules in the “primordial soup”? In the 1960s two of the components of RNA, adenine and guanine, were synthesized in the laboratory from simple molecules and energy sources thought to be present on early earth. In 1995 researchers discovered that, by adding the ...
Protein structural class prediction using predicted secondary
... Chapter 5 presents the results of the thesis work. This chapter includes the comparison of performance of various feature sets developed for this thesis. The comparison of performance of the best performing feature sets with some major published work is also presented in this chapter. Chapter 6 conc ...
... Chapter 5 presents the results of the thesis work. This chapter includes the comparison of performance of various feature sets developed for this thesis. The comparison of performance of the best performing feature sets with some major published work is also presented in this chapter. Chapter 6 conc ...
Improving protein fold recognition with hybrid
... structural information from solvent accessibility, secondary structure or backbone torsion angle can be used to improve sequencebased fold recognition. Such structural features have the advantage of being predictable, which addresses the absence of experimental model for the target protein. Thus, se ...
... structural information from solvent accessibility, secondary structure or backbone torsion angle can be used to improve sequencebased fold recognition. Such structural features have the advantage of being predictable, which addresses the absence of experimental model for the target protein. Thus, se ...
Dr: Mohamed I Kotb El
... With normal kidney function, then, the amount of creatinine in the blood remains relatively constant and normal. 3. Clinical Application of Creatine and Creatinine: Plasma creatinine concentration is a function of relative muscle mass, the rate of creatine turnover, and renal function. Measure ...
... With normal kidney function, then, the amount of creatinine in the blood remains relatively constant and normal. 3. Clinical Application of Creatine and Creatinine: Plasma creatinine concentration is a function of relative muscle mass, the rate of creatine turnover, and renal function. Measure ...
02b Basic equations two substrates
... In Cleland notation, the enzyme is represented by a horizontal line. Addition of substrate and release of product is represented by a vertical line. Enzyme intermediate species are indicated below the horizontal line and the rate constant for each step is indicated. In sequential ordered Bi Bi react ...
... In Cleland notation, the enzyme is represented by a horizontal line. Addition of substrate and release of product is represented by a vertical line. Enzyme intermediate species are indicated below the horizontal line and the rate constant for each step is indicated. In sequential ordered Bi Bi react ...
Enzymes:The Catalysts of Life I
... For most biologically important reactions at normal cellular temperatures, the activation energy is sufficiently high that the proportion of molecules possessing that much energy at any instant is extremely small. Accordingly, the rates of uncatalyzed reactions in cells are very low, and most molecu ...
... For most biologically important reactions at normal cellular temperatures, the activation energy is sufficiently high that the proportion of molecules possessing that much energy at any instant is extremely small. Accordingly, the rates of uncatalyzed reactions in cells are very low, and most molecu ...
The Biochemistry of Malic Acid Metabolism by Wine Yeasts
... TCA cycle. If the regeneration of NAD+ from NADH via the conversion of acetaldehyde to ethanol under fermentable conditions is restricted, the reductive arm of the TCA cycle can be used to regenerate NAD+, with the production of L-malate and succinate. In S. cerevisiae, the production of L-malate mo ...
... TCA cycle. If the regeneration of NAD+ from NADH via the conversion of acetaldehyde to ethanol under fermentable conditions is restricted, the reductive arm of the TCA cycle can be used to regenerate NAD+, with the production of L-malate and succinate. In S. cerevisiae, the production of L-malate mo ...
Urinalysis Monograph
... bacteria, Trichomonas vaginalis, Sperm cells, mucous filaments and crystals. Again, classify and report each element by a least-to-most range per HPF (High Power Field). Yeast, bacteria, mucous filaments and crystals are usually graded using the '+' notation (1+ = least significant amount, 4+ = most ...
... bacteria, Trichomonas vaginalis, Sperm cells, mucous filaments and crystals. Again, classify and report each element by a least-to-most range per HPF (High Power Field). Yeast, bacteria, mucous filaments and crystals are usually graded using the '+' notation (1+ = least significant amount, 4+ = most ...
RNA EXTRACTION
... What is RNA? • RNA = Ribonucleic acid. • A type of nucleic acid with only one strand - ribose instead of deoxyribose and using uracil instead of thymine (in DNA). • Provides the link between the genetic information through protein synthesis (serve as template for protein synthesis). • Total RNA= rR ...
... What is RNA? • RNA = Ribonucleic acid. • A type of nucleic acid with only one strand - ribose instead of deoxyribose and using uracil instead of thymine (in DNA). • Provides the link between the genetic information through protein synthesis (serve as template for protein synthesis). • Total RNA= rR ...
Nutritional Glossary and other plant
... Nutrients are needed for life. They are carbohydrates, proteins, fats, water, vitamins, and minerals. The body uses nutrients to grow, work, and fix itself. Calories Calories measure the energy found in food. Just as minutes measure time and inches measure length, calories measure the amount of ener ...
... Nutrients are needed for life. They are carbohydrates, proteins, fats, water, vitamins, and minerals. The body uses nutrients to grow, work, and fix itself. Calories Calories measure the energy found in food. Just as minutes measure time and inches measure length, calories measure the amount of ener ...
OptCDR: a general computational method for the design
... 2000) method and then expanded to include amino acids with similar chemical properties. When these sequence-based rules were not sufficient (25% of cases), the framework of the antibody variable region was aligned to a consensus framework and structural analysis was used to identify the CDRs. Throu ...
... 2000) method and then expanded to include amino acids with similar chemical properties. When these sequence-based rules were not sufficient (25% of cases), the framework of the antibody variable region was aligned to a consensus framework and structural analysis was used to identify the CDRs. Throu ...
Biochemistry
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Biochemistry, sometimes called biological chemistry, is the study of chemical processes within and relating to living organisms. By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism, biochemical processes give rise to the complexity of life. Over the last decades of the 20th century, biochemistry has become so successful at explaining living processes that now almost all areas of the life sciences from botany to medicine to genetics are engaged in biochemical research. Today, the main focus of pure biochemistry is in understanding how biological molecules give rise to the processes that occur within living cells, which in turn relates greatly to the study and understanding of whole organisms.Biochemistry is closely related to molecular biology, the study of the molecular mechanisms by which genetic information encoded in DNA is able to result in the processes of life. Depending on the exact definition of the terms used, molecular biology can be thought of as a branch of biochemistry, or biochemistry as a tool with which to investigate and study molecular biology.Much of biochemistry deals with the structures, functions and interactions of biological macromolecules, such as proteins, nucleic acids, carbohydrates and lipids, which provide the structure of cells and perform many of the functions associated with life. The chemistry of the cell also depends on the reactions of smaller molecules and ions. These can be inorganic, for example water and metal ions, or organic, for example the amino acids which are used to synthesize proteins. The mechanisms by which cells harness energy from their environment via chemical reactions are known as metabolism. The findings of biochemistry are applied primarily in medicine, nutrition, and agriculture. In medicine, biochemists investigate the causes and cures of disease. In nutrition, they study how to maintain health and study the effects of nutritional deficiencies. In agriculture, biochemists investigate soil and fertilizers, and try to discover ways to improve crop cultivation, crop storage and pest control.