Recognition of an organism from fragments of its complete genome
... genome and considered three kinds of length sequences. After calculating the correlation dimensions and Hurst exponents, it was found that one can get more information from this model than that of the fractal Cantor pattern. Some results on the classification and evolution relationship of bacteria w ...
... genome and considered three kinds of length sequences. After calculating the correlation dimensions and Hurst exponents, it was found that one can get more information from this model than that of the fractal Cantor pattern. Some results on the classification and evolution relationship of bacteria w ...
Topologically Non-linked Circular Duplex DNA
... These authors believe that circular DNA is not, in general, topologically helical, but rather has a structure not unlike those proposed by Rodley et al. (1976) and Sasisekharan et al. (1978), in which the two individual single-stranded circular half-chromosomes twist about each other alternately to ...
... These authors believe that circular DNA is not, in general, topologically helical, but rather has a structure not unlike those proposed by Rodley et al. (1976) and Sasisekharan et al. (1978), in which the two individual single-stranded circular half-chromosomes twist about each other alternately to ...
Comparison of the separation of Candida albicans chromosome
... of the chromosomal bands of C. albicans fell between S. cerevisiae AB972 chromosome Xin and 5. pombe 4660 chromosomes. The comigration of C. albicans H317 and 1012A bands with two unresolved S. pombe chromosomes (8), both of which are greater than 3.5 megabase pairs, suggests that the C. albicans ba ...
... of the chromosomal bands of C. albicans fell between S. cerevisiae AB972 chromosome Xin and 5. pombe 4660 chromosomes. The comigration of C. albicans H317 and 1012A bands with two unresolved S. pombe chromosomes (8), both of which are greater than 3.5 megabase pairs, suggests that the C. albicans ba ...
Inheritance of Organelle DNA Sequences in a Citrus–Poncirus
... Results and Discussion To identify DNA polymorphisms between LB 1–18 and P. trifoliata, total cellular DNA samples were digested and hybridized with two chloroplast probes (petA and petD) and seven mitochondrial probes (atpA, cob, coxI, coxII, coxIII, rrn26, and atp9). Most hybridization profiles re ...
... Results and Discussion To identify DNA polymorphisms between LB 1–18 and P. trifoliata, total cellular DNA samples were digested and hybridized with two chloroplast probes (petA and petD) and seven mitochondrial probes (atpA, cob, coxI, coxII, coxIII, rrn26, and atp9). Most hybridization profiles re ...
RT-PCR Master Mix (2X)
... product. The 3' or reverse primer is complementary to the target RNA. The 5' or forward primer is complementary to the corresponding first strand of cDNA. In reverse transcription, the reverse primer initiates synthesis of the first strand of cDNA. In PCR, the forward primer initiates synthesis of the ...
... product. The 3' or reverse primer is complementary to the target RNA. The 5' or forward primer is complementary to the corresponding first strand of cDNA. In reverse transcription, the reverse primer initiates synthesis of the first strand of cDNA. In PCR, the forward primer initiates synthesis of the ...
Detection and analysis of disease-associated single nucleotide
... developments. Discovering disease-associated non-synonymous SNPs (nsSNPs) altering PTM sites can help to estimate the various PTM candidates involved in diseases, therefore, an integrated analysis between SNPs, PTMs and diseases is necessary. However, only a few types of PTMs affected by nsSNPs have ...
... developments. Discovering disease-associated non-synonymous SNPs (nsSNPs) altering PTM sites can help to estimate the various PTM candidates involved in diseases, therefore, an integrated analysis between SNPs, PTMs and diseases is necessary. However, only a few types of PTMs affected by nsSNPs have ...
Mendelian Genetics and Beyond Chapter 4 Study Prompts 1. What is a
... father could either be “Dd” or “DD”. In either case he could pass on the “D” allele for dimples to the woman (his daughter). 19. The Law of Independent Assortment describes that homologous chromosomes randomly align on either side of the equatorial in meiosis (for example all of dad’s chromosomes ar ...
... father could either be “Dd” or “DD”. In either case he could pass on the “D” allele for dimples to the woman (his daughter). 19. The Law of Independent Assortment describes that homologous chromosomes randomly align on either side of the equatorial in meiosis (for example all of dad’s chromosomes ar ...
Physical mapping shows that the unstable oxytetracycline gene
... described by Rausch et al. (1993) ; the same methods were used to construct the cosmid gene bank of the AseI-J band. The vector used (sCos-1 ; Evans et al., 1989) has T3 and T7 promoter sequences flanking the insert and the insert is also flanked by EcoRI sites. pBR328 (Bolivar et al., 1977) was use ...
... described by Rausch et al. (1993) ; the same methods were used to construct the cosmid gene bank of the AseI-J band. The vector used (sCos-1 ; Evans et al., 1989) has T3 and T7 promoter sequences flanking the insert and the insert is also flanked by EcoRI sites. pBR328 (Bolivar et al., 1977) was use ...
Genetics and Pedigrees Bio I
... Inheritance- the passing on of traits from one generation to the next Trait/Characteristic- any factor used to describe or distinguish ...
... Inheritance- the passing on of traits from one generation to the next Trait/Characteristic- any factor used to describe or distinguish ...
DNA and RNA extraction
... Typically, this provides sufficient DNA for at least 5 Southern blots. Digestion of DNA Although the design of any particular experiment may require the use of a specific restriction enzyme for Southern blot analysis of Physcomitrella DNA, it should be noted that some enzymes cleave Physcomitrella D ...
... Typically, this provides sufficient DNA for at least 5 Southern blots. Digestion of DNA Although the design of any particular experiment may require the use of a specific restriction enzyme for Southern blot analysis of Physcomitrella DNA, it should be noted that some enzymes cleave Physcomitrella D ...
Evaluation of genomic DNA from paraffin
... All genomes consist of genetic polymorphism, which are sequences that are found in two or more variants (alleles) within a population. If the variants persist in the population with allele frequencies above 1 % for the rarest allele it is called a genetic polymorphism. Single nucleotide polymorphism ...
... All genomes consist of genetic polymorphism, which are sequences that are found in two or more variants (alleles) within a population. If the variants persist in the population with allele frequencies above 1 % for the rarest allele it is called a genetic polymorphism. Single nucleotide polymorphism ...
Abundance of an mRNA is the average number of molecules per cell
... an extract prepared from a mutant cell. The assay identifies the component rendered inactive by the mutation. ...
... an extract prepared from a mutant cell. The assay identifies the component rendered inactive by the mutation. ...
Identification of a Novel Streptococcal Gene
... (32), rifamycins (14), and -lactams (39), are known to induce an SOS response that increases mutations and accelerates the evolution of antibiotic resistance in E. coli (14). Furthermore, it has been shown that the prevention of induction of the LexA-regulated SOS response inhibits the evolution of ...
... (32), rifamycins (14), and -lactams (39), are known to induce an SOS response that increases mutations and accelerates the evolution of antibiotic resistance in E. coli (14). Furthermore, it has been shown that the prevention of induction of the LexA-regulated SOS response inhibits the evolution of ...
Polymorphisms in the CRP and C1Q genes and - dr
... To investigate additional two SNPs within C1Q genes (rs913243 and rs172378) using PCR techniques ...
... To investigate additional two SNPs within C1Q genes (rs913243 and rs172378) using PCR techniques ...
Patterns of Inheritance
... Co-dominance •In this pattern much like incomplete dominance, neither allele is considered recessive •In a heterozygous condition where each type of allele is present the phenotype displays both traits from each ...
... Co-dominance •In this pattern much like incomplete dominance, neither allele is considered recessive •In a heterozygous condition where each type of allele is present the phenotype displays both traits from each ...
Analysis of sequence variations in the factor VIII gene in
... signal sites (9). In some cases patients sharing the same functional mutation have varying levels of circulating f8 activity. The aim of this project is therefore to investigate this further by screening the 5’ upstream region of f8, including the promoter region, for polymorphic changes in order to ...
... signal sites (9). In some cases patients sharing the same functional mutation have varying levels of circulating f8 activity. The aim of this project is therefore to investigate this further by screening the 5’ upstream region of f8, including the promoter region, for polymorphic changes in order to ...
An Introduction to Genetic Analysis Chapter 16 Mechanisms of Gene
... FMR-1-encoded protein.] Both NTMs and their daughters have a much larger number of repeats, ranging from 50 to 200. These increased repeats have been termed premutations. All premutation alleles are unstable. The males and females with symptoms of the disease, as well as many carrier females, have a ...
... FMR-1-encoded protein.] Both NTMs and their daughters have a much larger number of repeats, ranging from 50 to 200. These increased repeats have been termed premutations. All premutation alleles are unstable. The males and females with symptoms of the disease, as well as many carrier females, have a ...
Alteration by site-directed mutagenesis of the
... hydroxylapatite and heparin-agarose contained the RecB-K29Q, RecC, and RecD proteins, and a single major contaminant (Fig. 2). Analysis of the gel shown in Fig. 2 by densitometry showed that the contaminant was about 70% of the total protein in the preparation. The protein concentration in this samp ...
... hydroxylapatite and heparin-agarose contained the RecB-K29Q, RecC, and RecD proteins, and a single major contaminant (Fig. 2). Analysis of the gel shown in Fig. 2 by densitometry showed that the contaminant was about 70% of the total protein in the preparation. The protein concentration in this samp ...
Crystal structure of the nucleosome core particle at 2.8 Å
... between the two long, 8-turn α2 helices at their crossover. Their antiparallel arrangement places the L1 loop of one in juxtaposition with the L2 loop of the other. As the contact surfaces are offset towards the N terminus by one helical turn, the C terminus of each α2 helix extends further along th ...
... between the two long, 8-turn α2 helices at their crossover. Their antiparallel arrangement places the L1 loop of one in juxtaposition with the L2 loop of the other. As the contact surfaces are offset towards the N terminus by one helical turn, the C terminus of each α2 helix extends further along th ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.