Teacher`s guide - National Centre for Biotechnology Education
... Selection of a genetic condition There are two ways in which the exercise may be used. It can be used to illustrate a true medical condition and its inheritance, such as the single base change (point mutation) that can cause to sickle cell anaemia. Alternatively, if teachers do not wish to a ‘real’ ...
... Selection of a genetic condition There are two ways in which the exercise may be used. It can be used to illustrate a true medical condition and its inheritance, such as the single base change (point mutation) that can cause to sickle cell anaemia. Alternatively, if teachers do not wish to a ‘real’ ...
Conclusion Introduction Background The PTC Sensitivity Gene
... Phenylthiocarbamide (PTC). This is one of the best known genetic traits in the human population and historically has been the most popular teaching subject in inheritance. However, the classic PTC paper test falls short of differentiating between homozygous vs heterozygous in the taster alleles. Her ...
... Phenylthiocarbamide (PTC). This is one of the best known genetic traits in the human population and historically has been the most popular teaching subject in inheritance. However, the classic PTC paper test falls short of differentiating between homozygous vs heterozygous in the taster alleles. Her ...
Force spectroscopy of single DNA and RNA molecules Mark C
... for measuring small forces acting on single DNA and RNA molecules. In these experiments, one end of the molecule is held at a fixed position, while the other end of the molecule is extended at constant force or to a fixed position. These techniques include atomic force microscopy (AFM) [1], optical ...
... for measuring small forces acting on single DNA and RNA molecules. In these experiments, one end of the molecule is held at a fixed position, while the other end of the molecule is extended at constant force or to a fixed position. These techniques include atomic force microscopy (AFM) [1], optical ...
MCB 421-2006: Homologous Recombination
... plasmids. We can also say that both RecG and Ruv functions help recombination, but the specificity of their action is unclear. Epistatic analysis involves combining two mutations in a single organism and monitoring the resulting phenotype. “Epistasis” means “covering over”, and originally epistatic ...
... plasmids. We can also say that both RecG and Ruv functions help recombination, but the specificity of their action is unclear. Epistatic analysis involves combining two mutations in a single organism and monitoring the resulting phenotype. “Epistasis” means “covering over”, and originally epistatic ...
Ch. 10 Presentation
... does not code for any other amino acid, – nearly universal—the genetic code is shared by organisms from the simplest bacteria to the most complex plants and animals, and – without punctuation in that codons are adjacent to each ...
... does not code for any other amino acid, – nearly universal—the genetic code is shared by organisms from the simplest bacteria to the most complex plants and animals, and – without punctuation in that codons are adjacent to each ...
View Full Text-PDF
... COIII, ND5, ND4, Cyt b, ND1, 16S, 12S and A + T). Due to the recognition that trees based on mitochondrial gene may represent only a partial and biased view on the phylogeny of organisms, many nuclear genes have been analyzed for the phylogeny of arthropods. Nuclear ribosomal RNA genes have been wid ...
... COIII, ND5, ND4, Cyt b, ND1, 16S, 12S and A + T). Due to the recognition that trees based on mitochondrial gene may represent only a partial and biased view on the phylogeny of organisms, many nuclear genes have been analyzed for the phylogeny of arthropods. Nuclear ribosomal RNA genes have been wid ...
Downloadable Full Text
... 2C, and 2D). Whether excess extranuclear DNA may hinder the export of damaged nuclear DNA and thus promote DNA damage in the nucleus is an open question. To increase the burden of endogenous damaged DNA, we treated cells or mice with cytarabine (Ara-C), a nucleoside analog that causes replication st ...
... 2C, and 2D). Whether excess extranuclear DNA may hinder the export of damaged nuclear DNA and thus promote DNA damage in the nucleus is an open question. To increase the burden of endogenous damaged DNA, we treated cells or mice with cytarabine (Ara-C), a nucleoside analog that causes replication st ...
Closely related proteins MBD2 and MBD3 play distinctive but
... any of our assays. All results shown below were obtained with the mice derived from the 5B cell line. Northern blots showed a reduction of the Mbd2 transcript in heterozygous animals, and no normal Mbd2 transcript was detectable in (−/−) animals (Fig. 2c). RT–PCR of RNA derived from Mbd2(−/−) animal ...
... any of our assays. All results shown below were obtained with the mice derived from the 5B cell line. Northern blots showed a reduction of the Mbd2 transcript in heterozygous animals, and no normal Mbd2 transcript was detectable in (−/−) animals (Fig. 2c). RT–PCR of RNA derived from Mbd2(−/−) animal ...
Next generation sequencing
... oligonucleotides) correspond to each fluorophore. If first or second nucleotide (in dinucleotide) is known, colour is unambiguously related with the other nucleotide. Three next positions — degenerate nucleotides: 64 different versions for each particular dinucleotide. When ligated to the sequencing ...
... oligonucleotides) correspond to each fluorophore. If first or second nucleotide (in dinucleotide) is known, colour is unambiguously related with the other nucleotide. Three next positions — degenerate nucleotides: 64 different versions for each particular dinucleotide. When ligated to the sequencing ...
pEGFP-C1 - Newcastle University Staff Publishing Service
... pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitut ...
... pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitut ...
Document
... that allows the plasmid to replicate in the bacteria using the host DNA synthesis enzymes A promoter sequence for initiating transcription of the inserted gene A gene encoding a protein for antibiotic resistance, which allows for identification of bacteria that have taken in the plasmid ...
... that allows the plasmid to replicate in the bacteria using the host DNA synthesis enzymes A promoter sequence for initiating transcription of the inserted gene A gene encoding a protein for antibiotic resistance, which allows for identification of bacteria that have taken in the plasmid ...
High efficiency of site-directed mutagenesis mediated by a single
... annealed to the denatured plasmid and directs the synthesis of the mutant strand. After digestion with selection enzyme, the plasmid DNA is amplified into Escherichia coli strain BMH71-18 and subjected to a second digestion and amplification into the bacterial strain DH5α. A mutagenesis efficiency > ...
... annealed to the denatured plasmid and directs the synthesis of the mutant strand. After digestion with selection enzyme, the plasmid DNA is amplified into Escherichia coli strain BMH71-18 and subjected to a second digestion and amplification into the bacterial strain DH5α. A mutagenesis efficiency > ...
PDF version - Atlas of Genetics and Cytogenetics in Oncology and
... VI.3.3. During mitosis, separation of centromeres in dicentric chromosomes to opposite poles produces an anaphase bridge, followed by chromosome breakage, subsequent fusion of damaged ends, and promotion of additional BFB cycles VI.3.4. Recurring cycles of gene amplification can arise during acquisi ...
... VI.3.3. During mitosis, separation of centromeres in dicentric chromosomes to opposite poles produces an anaphase bridge, followed by chromosome breakage, subsequent fusion of damaged ends, and promotion of additional BFB cycles VI.3.4. Recurring cycles of gene amplification can arise during acquisi ...
Lab Recap: Miniprep (MP)
... So now you have you can just pour out the supernatant into another tube and call that your plasmid DNA right? WRONG. You might still have some cellular junk in your supernatant, so you have to find out a way to make sure that your miniprep is completely plasmid DNA. So, you will pour the supernat ...
... So now you have you can just pour out the supernatant into another tube and call that your plasmid DNA right? WRONG. You might still have some cellular junk in your supernatant, so you have to find out a way to make sure that your miniprep is completely plasmid DNA. So, you will pour the supernat ...
emboj2008205-sup
... CUP1, SFA1derivative of TP strains described in Narayanan et al., 2006. GAA/TTC repeats of length 20, 60, 120 and 230 were integrated into LYS2 in two orientations using the dellito perfetto technique (Storici et al., 2001). GAA repeats located on the plasmids (Krasilnikova and Mirkin, 2004) were ...
... CUP1, SFA1derivative of TP strains described in Narayanan et al., 2006. GAA/TTC repeats of length 20, 60, 120 and 230 were integrated into LYS2 in two orientations using the dellito perfetto technique (Storici et al., 2001). GAA repeats located on the plasmids (Krasilnikova and Mirkin, 2004) were ...
Electrokinetic Stretching of Tethered DNA
... Gel electrophoresis is a powerful and ubiquitous technique for separation of biopolymers, particularly DNA. However, the mechanisms involved in electrophoretic separations are not fully understood (Viovy, 2000; Zimm and Levene, 1992). Because conventional gel electrophoresis is slow and typically li ...
... Gel electrophoresis is a powerful and ubiquitous technique for separation of biopolymers, particularly DNA. However, the mechanisms involved in electrophoretic separations are not fully understood (Viovy, 2000; Zimm and Levene, 1992). Because conventional gel electrophoresis is slow and typically li ...
References - UTH e
... 1. PCR enables rapid amplification of template DNA for screening of uncharacterized mutations Because of its rapidity and simplicity, PCR is ideally suited to providing numerous DNA templates for mutation screening. Partial DNA sequences, at the genomic or the cDNA level, from a gene associated with ...
... 1. PCR enables rapid amplification of template DNA for screening of uncharacterized mutations Because of its rapidity and simplicity, PCR is ideally suited to providing numerous DNA templates for mutation screening. Partial DNA sequences, at the genomic or the cDNA level, from a gene associated with ...
Processivity of DNA polymerases: two mechanisms, one goal
... family contain a putative domain in a location similar to the one in T7 polymerase between helices H and H1. The polymerase of the E. coli bacteriophage T3 contains a thioredoxin-binding domain and thus may use thioredoxin as a processivity factor in a similar manner to T7. Similarly, the DNA polyme ...
... family contain a putative domain in a location similar to the one in T7 polymerase between helices H and H1. The polymerase of the E. coli bacteriophage T3 contains a thioredoxin-binding domain and thus may use thioredoxin as a processivity factor in a similar manner to T7. Similarly, the DNA polyme ...
Medical Genetics
... A cell may have hundreds or even thousands of mitochondria depending on the particular cell’s need for energy. (For example, the average human liver cell contains more than a thousand mitochondria.) ...
... A cell may have hundreds or even thousands of mitochondria depending on the particular cell’s need for energy. (For example, the average human liver cell contains more than a thousand mitochondria.) ...
Hydrogen autotrophy of Nocardia opaca strains is
... method of Marmur (1961) these linear plasmids were not detectable; this may be due to their sensitivity to shearing forces. On conventional agarose gel electrophoresis the linear plasmids formed a broad band located slightly above the largest A HindIII fragment (Fig. 2). In lysates of N . opaca obta ...
... method of Marmur (1961) these linear plasmids were not detectable; this may be due to their sensitivity to shearing forces. On conventional agarose gel electrophoresis the linear plasmids formed a broad band located slightly above the largest A HindIII fragment (Fig. 2). In lysates of N . opaca obta ...