Biology STAAR EOC Fall 2011
... TEK 11C: Summarize the role of microorganisms in both maintaining and disrupting the health of both organisms and ecosystems. (Supporting Standard) 38. Microorganisms, such as bacteria, play a vital role in maintaining and disrupting the health of both organisms and ecosystems. Bacteria live symbio ...
... TEK 11C: Summarize the role of microorganisms in both maintaining and disrupting the health of both organisms and ecosystems. (Supporting Standard) 38. Microorganisms, such as bacteria, play a vital role in maintaining and disrupting the health of both organisms and ecosystems. Bacteria live symbio ...
DNA Technology Notes (13.1 &13.2)
... Biologists use DNA technology to produce plants with many desirable traits. Genetically engineered cotton resists insect infestation of the bolls. Sweet-potato plants are resistant to a virus that could kill most of the African harvest. Rice plants with increased iron and vitamins ...
... Biologists use DNA technology to produce plants with many desirable traits. Genetically engineered cotton resists insect infestation of the bolls. Sweet-potato plants are resistant to a virus that could kill most of the African harvest. Rice plants with increased iron and vitamins ...
Lecture Notes
... • Understanding how genetic information is encoded and decoded - DNA, RNA, proteins and the genetic code • Understanding how the expression of genetic information is regulated in simpler and more complex organisms • Understanding how evolutionary changes in protein function and expression arise and ...
... • Understanding how genetic information is encoded and decoded - DNA, RNA, proteins and the genetic code • Understanding how the expression of genetic information is regulated in simpler and more complex organisms • Understanding how evolutionary changes in protein function and expression arise and ...
Genetic mechanisms
... DNA double helix (replication fork) Strands are kept separate by SSBP (single strand binding proteins) An RNA ___________is synthesized (primase) and “primes” (DNA polymerase III cannot initiate synthesis on its own) ...
... DNA double helix (replication fork) Strands are kept separate by SSBP (single strand binding proteins) An RNA ___________is synthesized (primase) and “primes” (DNA polymerase III cannot initiate synthesis on its own) ...
Large-Scale Purification Of Plasmids pRIT4501 and - RIT
... isopycnic points. The table also shows the density of phage λ DNA. After transferring the lysate to the ultracentrifuge tube, ethidium bromide is added. While ethidium bromide certainly facilitates collection of the DNA at the end of the centrifuge run by virtue of its fluorescence, it is actually a ...
... isopycnic points. The table also shows the density of phage λ DNA. After transferring the lysate to the ultracentrifuge tube, ethidium bromide is added. While ethidium bromide certainly facilitates collection of the DNA at the end of the centrifuge run by virtue of its fluorescence, it is actually a ...
Download: Genes, Genomics, and Chromosomes
... collection of exons within a larger gene. The coding regions for domains can be spliced in or out of the primary transcript by the process of alternative splicing. The resulting mRNAs encode different forms of the protein, known as isoforms. Alternative splicing is an important method for regulation ...
... collection of exons within a larger gene. The coding regions for domains can be spliced in or out of the primary transcript by the process of alternative splicing. The resulting mRNAs encode different forms of the protein, known as isoforms. Alternative splicing is an important method for regulation ...
Chapter 6A
... collection of exons within a larger gene. The coding regions for domains can be spliced in or out of the primary transcript by the process of alternative splicing. The resulting mRNAs encode different forms of the protein, known as isoforms. Alternative splicing is an important method for regulation ...
... collection of exons within a larger gene. The coding regions for domains can be spliced in or out of the primary transcript by the process of alternative splicing. The resulting mRNAs encode different forms of the protein, known as isoforms. Alternative splicing is an important method for regulation ...
Isolation of DNA from A Single Helminth Using New Developed Kit
... reliable. The amount of the extracted DNA was low, when the worms were used for the DNA extraction promptly after removing from 70% ethanol solution. The important critical point for the DNA extraction is to ensure that the worm is well disrupted, homogenized, the cells are completely lysed and the ...
... reliable. The amount of the extracted DNA was low, when the worms were used for the DNA extraction promptly after removing from 70% ethanol solution. The important critical point for the DNA extraction is to ensure that the worm is well disrupted, homogenized, the cells are completely lysed and the ...
Yeast DNA Prep (Quick) Formosa
... E. coli, or for Southern Blots. These applications require different variations of the procedure, so the protocol diverges into three endings. 1. Grow yeast cultures to saturation overnight in 5 ml of YM-1 (+glucose) on a roller at the appropriate temperature (usually 30°). If recovering a plasmid t ...
... E. coli, or for Southern Blots. These applications require different variations of the procedure, so the protocol diverges into three endings. 1. Grow yeast cultures to saturation overnight in 5 ml of YM-1 (+glucose) on a roller at the appropriate temperature (usually 30°). If recovering a plasmid t ...
overview - El Paso High School
... Francis Crick and James Watson used model building and combined all the knowledge of DNA to determine its structure. Franklin’s X-ray crystallography convinced them the molecule was helical. Modeling also showed that DNA strands are anti-parallel. (VIDEO 9.1 Deoxyribonucleic acid: A three-dimension ...
... Francis Crick and James Watson used model building and combined all the knowledge of DNA to determine its structure. Franklin’s X-ray crystallography convinced them the molecule was helical. Modeling also showed that DNA strands are anti-parallel. (VIDEO 9.1 Deoxyribonucleic acid: A three-dimension ...
Perl Laboratory Study Guide – Section I
... Loop through the bases, keeping count of the appropriate number of nucleotides. foreach $base (@dna) { if ($base eq ‘A’) { ++$A_Number; } elsif ($base eq ‘C’) { ++$C_Number; } elsif ($base eq ‘G’) { ++$G_Number; } elsif ($base eq ‘T’) { ++$T_Number; } else { print “Error: I don’t recognize the bases ...
... Loop through the bases, keeping count of the appropriate number of nucleotides. foreach $base (@dna) { if ($base eq ‘A’) { ++$A_Number; } elsif ($base eq ‘C’) { ++$C_Number; } elsif ($base eq ‘G’) { ++$G_Number; } elsif ($base eq ‘T’) { ++$T_Number; } else { print “Error: I don’t recognize the bases ...
standard set 5 - EDHSGreenSea.net
... subunits: a five-carbon pentose sugar, a phosphoric acid group, and one of four nitrogen bases. (For DNA these nitrogen bases are adenine, guanine, cytosine, or thymine.) DNA and RNA differ in a number of major ways. A DNA nucleotide contains a deoxyribose sugar, but RNA contains ribose sugar. The n ...
... subunits: a five-carbon pentose sugar, a phosphoric acid group, and one of four nitrogen bases. (For DNA these nitrogen bases are adenine, guanine, cytosine, or thymine.) DNA and RNA differ in a number of major ways. A DNA nucleotide contains a deoxyribose sugar, but RNA contains ribose sugar. The n ...
DNA Replication
... For example, the DNA in a cell may establish that it is a human cell. If, during development, it becomes a cell in the iris of an eye, the DNA will direct other information appropriate for its location in the organism, such as the production of brown, blue, or other pigmentation. DNA controls the ce ...
... For example, the DNA in a cell may establish that it is a human cell. If, during development, it becomes a cell in the iris of an eye, the DNA will direct other information appropriate for its location in the organism, such as the production of brown, blue, or other pigmentation. DNA controls the ce ...
File
... Deletions are mutations in which a section of DNA is lost, or deleted. Frameshift Since protein-coding DNA is divided into codons three bases long, insertions and deletions can alter a gene so that its message is no longer correctly analyzed. These changes are called frameshifts. 2. What causes gene ...
... Deletions are mutations in which a section of DNA is lost, or deleted. Frameshift Since protein-coding DNA is divided into codons three bases long, insertions and deletions can alter a gene so that its message is no longer correctly analyzed. These changes are called frameshifts. 2. What causes gene ...
Structure of a DNA polymerase
... * Topoisomerases II change the linking number in steps of 2 by passing both strands of double-stranded DNA through a break. * Eukaryotic topoisomerases isolated to date only relax supercoiled DNA, while prokaryotic topoisomerases (gyrases) can, given ATP, add supercoils. * TopoII releases catenated ...
... * Topoisomerases II change the linking number in steps of 2 by passing both strands of double-stranded DNA through a break. * Eukaryotic topoisomerases isolated to date only relax supercoiled DNA, while prokaryotic topoisomerases (gyrases) can, given ATP, add supercoils. * TopoII releases catenated ...
Ess | Rebekah Ess Biology Lab November 2, 2012 “Genomic DNA
... should be classified as different taxonomic species based on their divergence 6.6-8.8 Mya. The Asian elephant has been determined to be the sister species of the woolly mammoth. While these studies are new and eye opening to the taxonomic status, the accuracy may be off as the use of mtDNA is only m ...
... should be classified as different taxonomic species based on their divergence 6.6-8.8 Mya. The Asian elephant has been determined to be the sister species of the woolly mammoth. While these studies are new and eye opening to the taxonomic status, the accuracy may be off as the use of mtDNA is only m ...
Recombinant DNA Technology Lecture Notes
... Only some of the bacteria take up a plasmid—How do you know which ones did? Not all plasmids are recombinant plasmids—How do you find those that are? Only some of plasmids contain the gene of interest—How do you identify these? ...
... Only some of the bacteria take up a plasmid—How do you know which ones did? Not all plasmids are recombinant plasmids—How do you find those that are? Only some of plasmids contain the gene of interest—How do you identify these? ...
Chapter 4 Molecular Cloning Methods
... with BamHI. This produces sticky ends with 5’-phosphates(red). Step 2: We remove the phosphates with alkaline phosphatase, making it impossible for the vector to re-ligate with itself. Step 3: We also cut the insert(yellow, upper right) with BamHI, producing sticky ends with phosphates that we do no ...
... with BamHI. This produces sticky ends with 5’-phosphates(red). Step 2: We remove the phosphates with alkaline phosphatase, making it impossible for the vector to re-ligate with itself. Step 3: We also cut the insert(yellow, upper right) with BamHI, producing sticky ends with phosphates that we do no ...
PCR amplification of the bacterial genes coding for nucleic acid
... d. Scroll down to the ‘Basic BLAST’ section to choose a BLAST program; click on the ‘nucleotide blast’ hyperlink text to access the nucleotide-nucleotide sequence analysis section of BLAST e. In the ‘Enter Query Sequence’ window, type in the DNA sequence you received with your worksheet hand-out - w ...
... d. Scroll down to the ‘Basic BLAST’ section to choose a BLAST program; click on the ‘nucleotide blast’ hyperlink text to access the nucleotide-nucleotide sequence analysis section of BLAST e. In the ‘Enter Query Sequence’ window, type in the DNA sequence you received with your worksheet hand-out - w ...
Question How does DNA control a cell?By controlling Protein
... Adds nucleotides to 3` end of growing RNA strand Enzyme moves 5` 3` (of RNA strand) Rate is about 60 nucleotides per second ...
... Adds nucleotides to 3` end of growing RNA strand Enzyme moves 5` 3` (of RNA strand) Rate is about 60 nucleotides per second ...
Chapter 4: Cytogenetics
... replicated copies of two homologous chromosomes carrying different alleles for genes A and B. Recombination between two of these molecules begins when an endonuclease nicks one strand of a double helix and unwinds the DNA. The nicked strand invades the neighboring homologous chromosome. In bacteria, ...
... replicated copies of two homologous chromosomes carrying different alleles for genes A and B. Recombination between two of these molecules begins when an endonuclease nicks one strand of a double helix and unwinds the DNA. The nicked strand invades the neighboring homologous chromosome. In bacteria, ...
Chapter 8: From DNA to Proteins
... When DNA is bombarded with x-rays, the atoms in DNA diffract the x-rays in a pattern that can be captured on film. Franklin’s data gave Watson and Crick what they needed to ultimately figure out the structure of DNA. The Double Helix Watson and Crick made many models using metal and wood to fi ...
... When DNA is bombarded with x-rays, the atoms in DNA diffract the x-rays in a pattern that can be captured on film. Franklin’s data gave Watson and Crick what they needed to ultimately figure out the structure of DNA. The Double Helix Watson and Crick made many models using metal and wood to fi ...
Table of Contents
... • If the amino acid sequence of a protein is known, it is possible to synthesize a DNA that can code for the protein. • Using the knowledge of the genetic code and known amino acid sequences, the most likely base sequence for the gene may be found. • Often sequences are added to this sequence to pro ...
... • If the amino acid sequence of a protein is known, it is possible to synthesize a DNA that can code for the protein. • Using the knowledge of the genetic code and known amino acid sequences, the most likely base sequence for the gene may be found. • Often sequences are added to this sequence to pro ...
Nucleic acid double helix
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.