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GPI Anchor
GPI Anchor

... Hyperacetylated Chromatin Domains 1.In eukaryotes, the genome is packaged into two general types of chromatin: heterochromatin, which appears compact or condensed throughout the cell cycle, and euchromatin, which appears condensed only prior to mitosis. 2.A small number of loci that exhibit covalen ...
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How to Claim your Biotech-Based Invention

... • antisense/target protein interactions • lack of correlation between in vitro and in vivo – efficient delivery to cells and cell targeting for specific disorders – oligo affinity/stability in vivo – “modulation” of target • inhibition • up-regulation – in vitro (cell culture) results generally  in ...
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Chapter 5 - Fernando Haro

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...  in nutrient and metabolite transporters.  in enzymes for reoxidation of NADH  petites reconfigure metabolism by recruiting peroxisomal activities, small molecule transport systems and lipid, sugar and amino acid turnover to get more OAA and Acetyl-CoA. ...
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... primers by disrupting the hydrogen bonds between complementary bases of the DNA strands, yielding single strands of DNA. Annealing step: The reaction temperature is lowered to 50-65°C for 20-40 seconds allowing annealing of the primers to the single-stranded DNA template. Typically the annealing tem ...
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Viral Ion Channels
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... • In denaturation, the weak hydrogen, electrostatic, and disulfide bonds are broken. • The bonds are weak enough that denaturation can occur from something as gentle as pouring a protein. • It can also occur from heating, whipping, and ultraviolet radiation, or from using strong acids and bases, alc ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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