Ho - Engineering Computing Facility
... survive and thrive. Consequently, profiling the transcriptome (mRNA) indicates how cells react to better their own chances at survival, or to protect surrounding tissues. Although several methods exist to measure gene expression, serial analysis of gene expression (SAGE) is unique in its ability to ...
... survive and thrive. Consequently, profiling the transcriptome (mRNA) indicates how cells react to better their own chances at survival, or to protect surrounding tissues. Although several methods exist to measure gene expression, serial analysis of gene expression (SAGE) is unique in its ability to ...
Review of the reconstruction
... Figures 43, 44, 45, 46, 47, 49, 50 and 52 in Additional file 6). The regulation of the synthesis of di- and tri-phosphate nucleotides (NDP, NTP) from mono-phosphate nucleotides (NMP) remains an open question. ATP seems to be the main phosphate donor. The synthesis of NDP from NMP and ATP is catalyze ...
... Figures 43, 44, 45, 46, 47, 49, 50 and 52 in Additional file 6). The regulation of the synthesis of di- and tri-phosphate nucleotides (NDP, NTP) from mono-phosphate nucleotides (NMP) remains an open question. ATP seems to be the main phosphate donor. The synthesis of NDP from NMP and ATP is catalyze ...
Classical genetics
... Britain’s King George III had lots of problems, they may have all been due to this genetic disease. ...
... Britain’s King George III had lots of problems, they may have all been due to this genetic disease. ...
Serological and molecular techniques to detect and identify plant
... highly specific and can detect variation within serogroups constructed through the use of polyclonal antibodies. However, their production is labour intensive and they may be too specific for some purposes so that related vimses may not be detected. The formation of antibody-antigen complexes causes ...
... highly specific and can detect variation within serogroups constructed through the use of polyclonal antibodies. However, their production is labour intensive and they may be too specific for some purposes so that related vimses may not be detected. The formation of antibody-antigen complexes causes ...
Identification of a novel testis‐specific gene and its potential roles in
... other cell types. Most of these genes also exhibit stagespecific expression during spermatogenesis, which could be considered as spermatogenic cell type-specific since the occurrence of different spermatogenic cell types is also stage-specific during spermatogenesis. Therefore, the spermatogenic cel ...
... other cell types. Most of these genes also exhibit stagespecific expression during spermatogenesis, which could be considered as spermatogenic cell type-specific since the occurrence of different spermatogenic cell types is also stage-specific during spermatogenesis. Therefore, the spermatogenic cel ...
video slide
... • A restriction enzyme will usually make many cuts in a DNA molecule yielding a set of restriction fragments • The most useful restriction enzymes cut DNA in a staggered way producing fragments with “sticky ends” that can bond with complementary “sticky ends” of other fragments • DNA ligase is an en ...
... • A restriction enzyme will usually make many cuts in a DNA molecule yielding a set of restriction fragments • The most useful restriction enzymes cut DNA in a staggered way producing fragments with “sticky ends” that can bond with complementary “sticky ends” of other fragments • DNA ligase is an en ...
Pfu DNA Polymerase Product Information 9PIM774
... 2. If using a thermal cycler without a heated lid, overlay the reaction mix with 1–2 drops (approximately 50µl) of mineral oil to prevent evaporation during thermal cycling. Centrifuge the reaction mix in a microcentrifuge for 5 seconds. 3. Immediately place the reactions in a thermal cycler that ha ...
... 2. If using a thermal cycler without a heated lid, overlay the reaction mix with 1–2 drops (approximately 50µl) of mineral oil to prevent evaporation during thermal cycling. Centrifuge the reaction mix in a microcentrifuge for 5 seconds. 3. Immediately place the reactions in a thermal cycler that ha ...
Analysis of DNA polymerase activity in vitro using non
... ABSTRACT. Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used ...
... ABSTRACT. Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used ...
BCH364C-391L-HMMs-Spring2015
... random, but that the sequence we observe is one of many possible instances of some underlying process or object. E.g., actin differs slightly from organism to organism. Imagine an “ideal”, but unobservable, actin, defined by certain underlying specific physico-chemical properties important for its f ...
... random, but that the sequence we observe is one of many possible instances of some underlying process or object. E.g., actin differs slightly from organism to organism. Imagine an “ideal”, but unobservable, actin, defined by certain underlying specific physico-chemical properties important for its f ...
Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell
... Cultures were fed with a defined mineral medium that limited growth by glucose or ethanol. The medium composition was based on that used by Verduyn et al. (1992). The concentration of substrate carbon in the reservoir medium was 250 mmol C l21 for ethanol-limited cultures and 830 mmol C l21 for gluc ...
... Cultures were fed with a defined mineral medium that limited growth by glucose or ethanol. The medium composition was based on that used by Verduyn et al. (1992). The concentration of substrate carbon in the reservoir medium was 250 mmol C l21 for ethanol-limited cultures and 830 mmol C l21 for gluc ...
Targeting the GAA-Repeat Region with Oligonucleotides for the
... this novel ncRNA, we have used locked nucleic acid “gapmer” oligonucleotides consisting of LNA ends and a central DNA stretch to degrade the putative ncRNA by an RNase H-mediated process. Targeting this ncRNA led to FXN mRNA and protein upregulation at therapeutically significant levels in FRDA pati ...
... this novel ncRNA, we have used locked nucleic acid “gapmer” oligonucleotides consisting of LNA ends and a central DNA stretch to degrade the putative ncRNA by an RNase H-mediated process. Targeting this ncRNA led to FXN mRNA and protein upregulation at therapeutically significant levels in FRDA pati ...
access full article - Caister Academic Press
... genes and the phenotypes of representative mutants are shown in figures 1A and 1B, respectively. Initial homology determinations were performed using BLAST (Altschul et al., 1990). The product of orf1 was weakly similar (61%; 59 a.a.) to phospholipase C precursor protein (Swissprot acession number P ...
... genes and the phenotypes of representative mutants are shown in figures 1A and 1B, respectively. Initial homology determinations were performed using BLAST (Altschul et al., 1990). The product of orf1 was weakly similar (61%; 59 a.a.) to phospholipase C precursor protein (Swissprot acession number P ...
Complete sequence analysis of the genome of the bacterium
... lipoprotein sequence pattern (46 cases) or ATP- and GTP-binding sites (73 cases). In addition all predicted gene products were analyzed by programs for structure predictions, e.g. coiled/coiled structures (29 cases) or transmembrane segments (275 cases). The latter result supports the analysis of ce ...
... lipoprotein sequence pattern (46 cases) or ATP- and GTP-binding sites (73 cases). In addition all predicted gene products were analyzed by programs for structure predictions, e.g. coiled/coiled structures (29 cases) or transmembrane segments (275 cases). The latter result supports the analysis of ce ...
Genes & Inheritance Series: Set 3 Copyright © 2005 Version: 2.0
... Cells need to control the rate and frequency of protein synthesis. These controls often occur at transcription. Sometimes genes are induced (and therefore transcribed) only when an enzyme product is required to catalyze reactions that may occur infrequently, e.g. use of a particular substrate that i ...
... Cells need to control the rate and frequency of protein synthesis. These controls often occur at transcription. Sometimes genes are induced (and therefore transcribed) only when an enzyme product is required to catalyze reactions that may occur infrequently, e.g. use of a particular substrate that i ...
6. DNA transcription/translation
... • The 46 chromosomes of a human cell contain about 6 BILLION base pairs of DNA!! – Printed one letter (A,C,T,G) at a time…would fill up over 900 volumes of Campbell. – Takes a cell a few hours to copy this DNA ...
... • The 46 chromosomes of a human cell contain about 6 BILLION base pairs of DNA!! – Printed one letter (A,C,T,G) at a time…would fill up over 900 volumes of Campbell. – Takes a cell a few hours to copy this DNA ...
12_Lecture_Presentation
... A genomic library is a collection of all of the cloned DNA fragments from a target genome Genomic libraries can be constructed with different types of vectors – Plasmid library: genomic DNA is carried by plasmids – Phage library: genomic DNA is incorporated into bacteriophage DNA ...
... A genomic library is a collection of all of the cloned DNA fragments from a target genome Genomic libraries can be constructed with different types of vectors – Plasmid library: genomic DNA is carried by plasmids – Phage library: genomic DNA is incorporated into bacteriophage DNA ...
Primary sequence analysis of Xac catalases
... identity) [6], which have not been characterized so far (Figure S3). Amino acid sequence ...
... identity) [6], which have not been characterized so far (Figure S3). Amino acid sequence ...
the Acetyl-Coenzyme A
... an unusual context for a translational start site, while the next ATG. 24 codons downstream. is in a more conventional context. Possible implications of two alternative translational start sites for the cellular localization o r the enzyme are discussed. A stable mutant of this gene. obtained by the ...
... an unusual context for a translational start site, while the next ATG. 24 codons downstream. is in a more conventional context. Possible implications of two alternative translational start sites for the cellular localization o r the enzyme are discussed. A stable mutant of this gene. obtained by the ...
Biofuel phyto-forensics case resolved through PCR
... that such sorghum can be toxic to consumers. He breaks the lock and takes away the bag of Bio-Fuel sorghum seeds. On returning home that evening BF discovers the break ins and immediately suspects TF for the theft due to history of their encounters. At the investigation agency, Mario (the geneticist ...
... that such sorghum can be toxic to consumers. He breaks the lock and takes away the bag of Bio-Fuel sorghum seeds. On returning home that evening BF discovers the break ins and immediately suspects TF for the theft due to history of their encounters. At the investigation agency, Mario (the geneticist ...
Recombinant DNA Technology
... sequences that are repeated several times in tandem: TGTGTGTGTGTG is 6 tandem repeats of TG. SSRs are found in and near many genes throughout the genome--they are quite common and easy to find. During normal replication of the DNA in the nucleus, DNA polymerase sometimes slips and creates extra copi ...
... sequences that are repeated several times in tandem: TGTGTGTGTGTG is 6 tandem repeats of TG. SSRs are found in and near many genes throughout the genome--they are quite common and easy to find. During normal replication of the DNA in the nucleus, DNA polymerase sometimes slips and creates extra copi ...
Luciferase Reporter Vector Protocol
... Overview Eukaryotic gene expression is regulated by a wide variety of developmental and environmental stimuli. First, an extracellular signaling molecule binds to a specific receptor. The signal is then transmitted through a series of molecular cascades, which activate or deactivate specific transcr ...
... Overview Eukaryotic gene expression is regulated by a wide variety of developmental and environmental stimuli. First, an extracellular signaling molecule binds to a specific receptor. The signal is then transmitted through a series of molecular cascades, which activate or deactivate specific transcr ...
91.510_ch06 - Computer Science
... not necessarily mean the score is biologically significant, only that the MSP was more likely to have been generated from the target distribution, which presumably was chosen on the basis of some interesting biological phenomena (such as multiple alignments of families of protein sequences) Further ...
... not necessarily mean the score is biologically significant, only that the MSP was more likely to have been generated from the target distribution, which presumably was chosen on the basis of some interesting biological phenomena (such as multiple alignments of families of protein sequences) Further ...
Minor Groove to Major Groove, an Unusual DNA Sequence
... explained in a logical manner. While they do not provide alternative explanations for any of their data, their reasoning is quite convincing, and it would be difficult to interpret their findings differently. Furthermore, not only does each method have stringent controls, the resulting data obtained ...
... explained in a logical manner. While they do not provide alternative explanations for any of their data, their reasoning is quite convincing, and it would be difficult to interpret their findings differently. Furthermore, not only does each method have stringent controls, the resulting data obtained ...
Parallel Analysis of Gene Expression: Microarrays
... that is likely to be representative of genes expressed under different conditions. It is possible to generate a cDNA microarray simply from randomly picked, unsequenced, clones. This leads to overrepresentation of a small subset of highly expressed genes on the microarray; however, molecular subtra ...
... that is likely to be representative of genes expressed under different conditions. It is possible to generate a cDNA microarray simply from randomly picked, unsequenced, clones. This leads to overrepresentation of a small subset of highly expressed genes on the microarray; however, molecular subtra ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.