Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... and 3' ends of the HKB structural gene were calculated to be roughly 1170 nucleotides upstream and 210 downstream from the PstI site. Since a number of restriction sites had already been identified in these regions, they were used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy seque ...
... and 3' ends of the HKB structural gene were calculated to be roughly 1170 nucleotides upstream and 210 downstream from the PstI site. Since a number of restriction sites had already been identified in these regions, they were used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy seque ...
DNA Replication
... • If a cell replicates in 10 hours how many bases per second does replication proceed at? ...
... • If a cell replicates in 10 hours how many bases per second does replication proceed at? ...
DNA replication - Olympic High School
... • If a cell replicates in 10 hours how many bases per second does replication proceed at? ...
... • If a cell replicates in 10 hours how many bases per second does replication proceed at? ...
P.abyssi PDF version
... fit into any COG category, eventually leaving approximately one-third (580) orphan sequences. Overall analysis results are summarized in Table 1. The global distribution of genes within different functional categories did not change significantly, the most salient feature remaining the net increase ...
... fit into any COG category, eventually leaving approximately one-third (580) orphan sequences. Overall analysis results are summarized in Table 1. The global distribution of genes within different functional categories did not change significantly, the most salient feature remaining the net increase ...
Forever 100bp Ladder Personalizer
... distinguished from any existing commercialized consumables 100 bp DNA ladder. This system supplies templates (plasmids) which will be used to amplify size markers. Since 12 differently sized fragments were cloned into plasmids, it has a unique feature of endless usage with this system. Another featu ...
... distinguished from any existing commercialized consumables 100 bp DNA ladder. This system supplies templates (plasmids) which will be used to amplify size markers. Since 12 differently sized fragments were cloned into plasmids, it has a unique feature of endless usage with this system. Another featu ...
Nucleosome depletion at yeast terminators is not
... gene. The heat map in Fig. 4A expresses H3 occupancy for divergent (Upper) or tandem (Lower) genes ordered by distance from the upstream neighbor. For promoter regions, the extent of nucleosome depletion is unaffected by distance to or orientation of the upstream genes. At both tandem and divergentl ...
... gene. The heat map in Fig. 4A expresses H3 occupancy for divergent (Upper) or tandem (Lower) genes ordered by distance from the upstream neighbor. For promoter regions, the extent of nucleosome depletion is unaffected by distance to or orientation of the upstream genes. At both tandem and divergentl ...
ExoLocator—an online view into genetic makeup of vertebrate
... Some otherwise interesting peculiarities of animal genomes complicate systematic analysis of the sort we undertook here. Thus we do not attempt to resolve the cases of overlapping genes on the same strand, of which we detected several hundred cases (some of these, though, might be duplicate entries ...
... Some otherwise interesting peculiarities of animal genomes complicate systematic analysis of the sort we undertook here. Thus we do not attempt to resolve the cases of overlapping genes on the same strand, of which we detected several hundred cases (some of these, though, might be duplicate entries ...
AP® BIOLOGY 2009 SCORING GUIDELINES (Form B)
... 2009 SCORING GUIDELINES (Form B) Question 1 Describe how a plasmid can be genetically modified to include a piece of foreign DNA that alters the phenotype of bacterial cells transformed with the modified plasmid. Describe a procedure to determine which bacterial cells have been successfully transfor ...
... 2009 SCORING GUIDELINES (Form B) Question 1 Describe how a plasmid can be genetically modified to include a piece of foreign DNA that alters the phenotype of bacterial cells transformed with the modified plasmid. Describe a procedure to determine which bacterial cells have been successfully transfor ...
DNA Replication in Bacteria
... In some cases, however, the damage may not be repairable, or the advancing replication fork may already have unwound the parental strands, thus preventing excision mechanisms from using the complementary strand as template for repair, or excision repair may not yet have had an opportunity to repair ...
... In some cases, however, the damage may not be repairable, or the advancing replication fork may already have unwound the parental strands, thus preventing excision mechanisms from using the complementary strand as template for repair, or excision repair may not yet have had an opportunity to repair ...
Molecular changes associated with the setting up of secondary
... identical mechanisms and/or genes are involved in the identity and maintenance of the vascular meristem, compared with the apical meristem (Schrader et al., 2004; Ko and Han, 2004). In Arabidopsis, the onset of body weight-induced secondary growth has been investigated by microarray analysis. Severa ...
... identical mechanisms and/or genes are involved in the identity and maintenance of the vascular meristem, compared with the apical meristem (Schrader et al., 2004; Ko and Han, 2004). In Arabidopsis, the onset of body weight-induced secondary growth has been investigated by microarray analysis. Severa ...
Preferential expression of one P-tubulin gene during
... et al., 1980). We are studying the P-tubulin gene family of Physarum to try to understand how multiple tubulin genes may be of benefit to a simple microbial eukaryote. We show here that the multiple genes for P-tubulin show different patterns of expression in different cell types. Even two virtually ...
... et al., 1980). We are studying the P-tubulin gene family of Physarum to try to understand how multiple tubulin genes may be of benefit to a simple microbial eukaryote. We show here that the multiple genes for P-tubulin show different patterns of expression in different cell types. Even two virtually ...
... extraction preparations proved to be the most effective for transformation. However, in all cases the number of transformants obtained was substantially lower (one-fifth to one-twentieth) than the number that would be obtained with the same amount of CsCl gradient purified DNA. Relatively impure pla ...
Expression profiles of Ciona tailbud embryos - Development
... muscle. Many genes were also specifically expressed in multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were ...
... muscle. Many genes were also specifically expressed in multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were ...
Mining Large Heterogeneous Cancer Data Sets Using
... copy number alteration, DNA methylation and expression of genes, a new hierarchy of mutations and recurrent copy number alterations, loss-of-heterozygosity of well-known tumor suppressors, and the association between mutations and aberrant DNA hypermethylation in cancer. Below, we describe the appli ...
... copy number alteration, DNA methylation and expression of genes, a new hierarchy of mutations and recurrent copy number alterations, loss-of-heterozygosity of well-known tumor suppressors, and the association between mutations and aberrant DNA hypermethylation in cancer. Below, we describe the appli ...
10858_2015_9967_MOESM1_ESM
... in order to assess the generality of the approach. For transcriptions, standard primers were used to focus on homogeneity effects caused by DMSO and not 2’-O-methylation of the primers. For this study, we chose A/U rich sequences, which caused severe amounts of non-DNA-templated nucleotide addition ...
... in order to assess the generality of the approach. For transcriptions, standard primers were used to focus on homogeneity effects caused by DMSO and not 2’-O-methylation of the primers. For this study, we chose A/U rich sequences, which caused severe amounts of non-DNA-templated nucleotide addition ...
Kuever et al_final.p
... from a freshwater ditch and is of interest because it can grow with a large variety of organic substrates, in particular several aromatic compounds, short-chain and mediumchain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow autotrophical ...
... from a freshwater ditch and is of interest because it can grow with a large variety of organic substrates, in particular several aromatic compounds, short-chain and mediumchain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow autotrophical ...
DNA Sequencing
... mixture of DNA molecules, usually fragments produced by restriction enzyme digestion, is separated into “bands”; each band contains thousands of molecules of the same length. After the current is turned off, a DNA-binding dye is added. This dye fluoresces pink in ultraviolet light, revealing the sep ...
... mixture of DNA molecules, usually fragments produced by restriction enzyme digestion, is separated into “bands”; each band contains thousands of molecules of the same length. After the current is turned off, a DNA-binding dye is added. This dye fluoresces pink in ultraviolet light, revealing the sep ...
Merging Gene Expression and Methylation Data
... about Illumina DNA Methylation and RNA Analysis Solutions. ...
... about Illumina DNA Methylation and RNA Analysis Solutions. ...
chapter 20 notes
... mixture of DNA molecules, usually fragments produced by restriction enzyme digestion, is separated into “bands”; each band contains thousands of molecules of the same length. After the current is turned off, a DNA-binding dye is added. This dye fluoresces pink in ultraviolet light, revealing the sep ...
... mixture of DNA molecules, usually fragments produced by restriction enzyme digestion, is separated into “bands”; each band contains thousands of molecules of the same length. After the current is turned off, a DNA-binding dye is added. This dye fluoresces pink in ultraviolet light, revealing the sep ...
Genome-wide analysis of the GRAS gene family in
... distribution. The exon-intron arrangement and conserved domains and motifs of each gene were also characterized. The study also includes a phylogenetic analysis of GRAS proteins based on the complete set of GRAS proteins from the flowering plants physic nut, castor bean, Arabidopsis and rice, the ly ...
... distribution. The exon-intron arrangement and conserved domains and motifs of each gene were also characterized. The study also includes a phylogenetic analysis of GRAS proteins based on the complete set of GRAS proteins from the flowering plants physic nut, castor bean, Arabidopsis and rice, the ly ...
Nucleic Acids Research
... suggestions, these do not form part of the present recommendations. First, we consider the uncertainty as to whether a base exists at a certain position or not. A symbol denoting "G or A or T or C or no nucleotide", for example ? or +, might be used to define regions of uncertainty of limited variab ...
... suggestions, these do not form part of the present recommendations. First, we consider the uncertainty as to whether a base exists at a certain position or not. A symbol denoting "G or A or T or C or no nucleotide", for example ? or +, might be used to define regions of uncertainty of limited variab ...
Nucleotide sequence of the thioredoxin gene from
... the sequence of another Sau3A fragment (Fig. 2) from the thioredoxin gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separatio ...
... the sequence of another Sau3A fragment (Fig. 2) from the thioredoxin gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separatio ...
Chapter 10 Notes
... • Genes provide the instructions for making specific proteins. • The initial one gene–one enzyme hypothesis was based on studies of inherited metabolic diseases. • The one gene–one enzyme hypothesis was expanded to include all proteins. ...
... • Genes provide the instructions for making specific proteins. • The initial one gene–one enzyme hypothesis was based on studies of inherited metabolic diseases. • The one gene–one enzyme hypothesis was expanded to include all proteins. ...
DNA Sequencing
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
Second bioinformatics lab:Exercise on disease
... Second bioinformatics lab: Exercise on disease (developed in part by Sarah C. R. Elgin, Washington University) It is well known that smoking leads to an increased risk for lung cancer, but how does genetics play into the risk? The transformation of a normal cell into a cancerous cell can result from ...
... Second bioinformatics lab: Exercise on disease (developed in part by Sarah C. R. Elgin, Washington University) It is well known that smoking leads to an increased risk for lung cancer, but how does genetics play into the risk? The transformation of a normal cell into a cancerous cell can result from ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.