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DNA vaccination
DNA vaccination

... the antigen expression unit composed of promoter sequences AND antigenencoding and polyadenylation sequences (termination signal) ...
Bacteria stress responce to high pressure processing
Bacteria stress responce to high pressure processing

... • Doing a literature study on bacteria stress response to high pressure processing (HPP). • The bacteria response - change of gene expressions and mechanisms in the cell when stressed. Methodology • Using experimental data done with HPP at 400 Mpa and 600 Mpa with Listeria contaminated food • Find v ...
Messenger RNA (mRNA)
Messenger RNA (mRNA)

... Transfer RNA (tRNA): shuttles amino acids to the ribosomes. It's responsible for bringing the correct amino acids into place at the appropriate time. It does this by reading the message carried by the mRNA. Transcription: Copying of the genetic code directly from DNA. Only a piece of DNA is copied. ...
Topic 13: ORGANIZATION OF DNA INTO GENES AND THE
Topic 13: ORGANIZATION OF DNA INTO GENES AND THE

... messenger RNA or mRNA). RNA differs from DNA (fig. 5.29) in that it (a) is generally single stranded, (b) it has the pyrimidine base uracil instead of thymidine and (c) the sugar is ribose. 2. translation- the information carried in the RNA molecule is translated into a sequence of amino acids there ...
ppt presentation
ppt presentation

... determined. However, because they are long enough to convey sequence specificity yet small enough to move through plasmodesmata, it is possible that they are ...
No Slide Title
No Slide Title

... How holoenzyme finds a specific promoter (60bp in a 4x106 stretch)? The forward rate constant for RNA Pol binding to promoters is faster than random diffusion (that limits the constant to 108/M-1Sec-1). The measured rate constant for association with a 60 bp target is 1014/M-1Sec-1. If the target i ...
FlyCutTM XmaI - AP
FlyCutTM XmaI - AP

... FlyCutTM XmaI is expressed and purified from E.coli that carries the recombinant XmaI gene. The molecular weight is 37.6 kDa, with the recognition site at C^CCGGG. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or ...
Transcription and Translation: Protein synthesis
Transcription and Translation: Protein synthesis

... UUA CCC GCA UCG UAA ...
Warheit#2
Warheit#2

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gene - LICH
gene - LICH

... • Each of the following lines (indented at the same level) gives the name of a protein product, indicates the reading frame to use (here, 343 is the first base of the first codon), the genetic code to apply (/transl_table), and a number of IDs for the protein sequence. • /translation introduces the ...
Microarray - Clemson University
Microarray - Clemson University

... The exact match is a section of the mRNA sequence you wish to probe for The mismatch is identical except for one base difference from it’s exact match counterpart, and is used to calculate a background. There are typically 11 “probe pairs” scattered around the chip- called a probe set. By combining ...
DNA→ RNA
DNA→ RNA

... helix Coils Supercoils ...
Department of Drug Delivery Research
Department of Drug Delivery Research

... chemotherapy to control the distribution of drugs and it is one of the most important fields and/or basic technologies supporting drug discovery and development in the pharmaceutical sciences associated with biomedicine and gene medicine. We have already developed a targeting system for macromolecul ...
Prokaryotic Gene Regulation
Prokaryotic Gene Regulation

... • For some bacterial genes, the translation of mRNA is regulated by the binding of proteins • A translational regulatory protein recognizes sequences within the mRNA • In most cases, these proteins act to inhibit translation – These are known as translational repressors ...
“Gene Regulation in Prokaryotes”
“Gene Regulation in Prokaryotes”

... Negative Control of the lac Operon by the lacI Repressor lac repressor is allosteric: allosteric it has two different conformations 1. In presence of inducer, it does not bind DNA 2. In absence of inducer, it binds strongly to lac operator DNA lac I mutations: lac I- - no repressor function - const ...
reduce usage of proper splice site
reduce usage of proper splice site

... • Group I introns use a free G nucleotide to catalyze reaction • Group II splicing is similar reaction to that in pre-mRNA splicing ...
Algorithms for Bioinformatics Autumn 2010
Algorithms for Bioinformatics Autumn 2010

...  This course aims to educate bioinformaticians that ...
Wood-forming genes active in mosses Research Highlights
Wood-forming genes active in mosses Research Highlights

... Wood-forming genes active in mosses Key genes for wood development are expressed in mosses, hinting at an ancient evolutionary story ...
Stabilization of poly-L-lysine-based cancer
Stabilization of poly-L-lysine-based cancer

... We have proposed a novel gene targeting strategy, called “drug or gene delivery system responding to cellular signals (D-RECS)”. The systems can activate transgene in response to malfunctions of signal transduction occurring inside diseased cells. In the precious study we succeed in activating gene ...
From Gene to Protein
From Gene to Protein

... loops are characteristic of all tRNAs, as is the base sequence of the * G amino acid attachment site at the 3 end. The anticodon triplet is A A* unique to each tRNA type. (The asterisks mark bases that have been C U ...
RNA and Protein synthesis
RNA and Protein synthesis

... energy of an ATP molecule. • Once the ATP’s energy is used to create a high energy bond the tRNA and amino acid are released and then travels to the ribosome. • Video  ...
GM crops: Miracles or Monsters
GM crops: Miracles or Monsters

... Mention the possibility of moving genes from one species to another. Allow the students to examine the plants and to try to decide which have been genetically modified. They can then reveal the answer by lifting up the top sheet. If possible, use real examples of genetically modified and/or normal v ...
Lecture Notes
Lecture Notes

... Zn coordination is via 2 His and 2 Cys in the first class of Zn fingers discovered (C2H2 fingers) C2C2 versions also exist, as do C6 di-Zn2+-binding proteins. Zn fingers are usually modules of larger proteins. Proteins with as many as 37 Zn fingers are known. Their role is to bind specific sequences ...
Quick Links
Quick Links

... Mention the possibility of moving genes from one species to another. Allow the students to examine the plants and to try to decide which have been genetically modified. They can then reveal the answer by lifting up the top sheet. If possible, use real examples of genetically modified and/or normal v ...
large bases - De Anza College
large bases - De Anza College

... extremely detrimental because the final protein intended by the message may be altered or not made ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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