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DNA Technology - 2 What are plasmids?
DNA Technology - 2 What are plasmids?

... What happens when the bacterial cell replicates its chromosome? It also replicates the plasmid DNA (including any foreign DNA as well) ...
Protein synthesis and mut ppt
Protein synthesis and mut ppt

... same mRNA strip all at once  Polypeptides with specific destinations  Some polypeptides need to leave the cell  Therefore they are made in bound ribosome's on the ER and other membrane bound organelles for transport ...
Gene Ontology Annotation (UniProt-GOA) - EMBL-EBI
Gene Ontology Annotation (UniProt-GOA) - EMBL-EBI

... Manual annotation benefits Manual annotation provides the most specific and detailed annotations for gene products. One of our aims is to undertake focused annotation projects, to improve both the ontology and its association to gene products. Recent examples of this include annotation of proteins i ...
DNA Replication - Texas Tech University
DNA Replication - Texas Tech University

... Transcribe 5.8S, 18S and 28S genes S = Svedberg units, sedimentation coefficient related to centrifugation The bigger the number the bigger the molecule ...
cudaGSEA
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... scalable bioinformatics tools that can process largescale gene expression data sets • CUDA is a suitable technology to address this need • cudaGSEA on one GPU achieves around two orders-ofmagnitude speedup versus BroadGSEA on a CPU – analyzing 20,639 genes measured in 200 patients with 4,726 pathway ...
Recombinant reflectin-based camouflage materials
Recombinant reflectin-based camouflage materials

... BLR(DE3) defective in the expression of SlyD protein.6 Authenticity of the clones was confirmed by DNA sequencing. 5 ml of over night cultures were grown in LB medium with 30 μg/ml kanamycin at 37oC in a rotary shaker and were used for inoculation of 1 L of LB cultures with 30 μg/ml kanamycin at a r ...
Transcription - Winston Knoll Collegiate
Transcription - Winston Knoll Collegiate

... Protein Synthesis is the process that cells use to produce protein. - it involves 2 distinct phases Transcription – occurs in the nucleus involves the creation of mRNA Translation – occurs in the cytoplasm at a ribosome – the protein recipe is “read” and the correct protein is made ...
Molecular Biology of the Gene
Molecular Biology of the Gene

... • Recall that DNA is a double stranded molecule of nucleotides that are held together by hydrogen bonds between complimentary bases across the 2 strands – the coding strand and the template strand – T…A and G…C • Each molecule of DNA is subdivided into thousands of segments containing a specific seq ...
Molecular Biology
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Regulation of Ribosomal RNA Synthesis in E. coli: Effects of the
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... major effectors for the control of ribosomal RNA in bacteria. The effector molecules accumulate to different cellular levels at amino acid deprivation or during different growth rates. They change the activity of RNA polymerase to transcribe from sensitive promoters (e.g. ribosomal RNA promoters). S ...
Plant Biotechnology and GMOs
Plant Biotechnology and GMOs

... virtually any target cell or tissue. 3. The particles carry the DNA  cells do not have to be removed from tissue in order to transform the cells ...
13lctout - Evergreen Archives
13lctout - Evergreen Archives

... III. Transcription in Eukaryotes A. Eukaryotic RNA Polymerase—Three different RNA polymerases are present in every cell. (Table 13.1) 1. RNA polymerase I transcribes genes that code for ribosomal RNAs. 2. RNA polymerase II transcribes genes that code for proteins; thus it synthesizes mRNAs. 3. RNA p ...
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pruitt_ppt_ch07

... possible candidates who are likely to suffer from a disease based on their nucleotide sequences. ...
DNA Fingerprinting
DNA Fingerprinting

... •  DNA is the hereditary material and contains all the information needed to build an organism. •  It is a polymeric molecule made from discrete units called nucleotides. •  Nucleotides link together to form a DNA strand at positions 3’ and 5’ ...
Stress and Brain Development
Stress and Brain Development

... How do the steroid hormones mediate their varied responses? For each hormone, a specific receptor exists within the cell. It is important to note one fundamental difference, however, between the steroid hormones and the other signaling molecules. The receptors for growth factors and neurotransmitter ...
Bio 102 Practice Problems
Bio 102 Practice Problems

... primers that could be used to amplify only the hexokinase gene from a human DNA sample by PCR. b. In your initial attempt, you succeed in obtaining hexokinase DNA and ligating it into a plasmid vector, but when you transform the recombinant plasmid into bacteria, you get no hexokinase protein produc ...
DNA  RNA  Proteins - Aurora City Schools
DNA RNA Proteins - Aurora City Schools

...  1. Initiation Promoter is the nucleotide sequence on DNA that marks where transcription of a gene begins and ends; “start” signal  Promoter serves as a specific binding site for RNA polymerase and determines which of the two strands of the DNA double helix is used as the template.  Specific nuc ...
12-3 RNA and Protein Synthesis
12-3 RNA and Protein Synthesis

... Mutations are changes in the genetic material. Kinds of Mutations Mutations that produce changes in a single gene are known as gene mutations. Mutations that produce changes in whole chromosomes are known as chromosomal mutations. Gene Mutations Gene mutations involving a change in one or a few nucl ...
Increasing the vitamin E content in plants by overexpressing the γ
Increasing the vitamin E content in plants by overexpressing the γ

... Increasing the vitamin E content in plants by overexpressing the γtocopherol methyltransferase gene ...
2010 PCB 5530 Class Projects
2010 PCB 5530 Class Projects

... Start by identifying all the known metabolites, enzymes and their EC numbers, and transporters in the assigned pathway in plants, bacteria, yeast, and animals. Remember that some pathways have variants; be sure to include these. This work will yield the equivalent of a KEGG pathway map. Next, identi ...
Gene Section RBM5 (RNA binding motif protein 5) in Oncology and Haematology
Gene Section RBM5 (RNA binding motif protein 5) in Oncology and Haematology

... Full length RBM5 encodes a protein with a molecular mass of about 90 kDa (815 amino acids). The protein has two RNA Binding Domains (RBD), also recognized as RNA Recognition Motif (RRM). RBM5 structure also features other functional motifs, which includes two putative zinc-finger DNA binding motifs, ...
Here`s the Quiz answers! - The University of Sheffield
Here`s the Quiz answers! - The University of Sheffield

... binding complexes that regulate transcription (and some bind directly to DNA). What are transcription factors generally composed of? A.  B.  C.  D.  E.  ...
Chapter 17: Microbial taxonomy
Chapter 17: Microbial taxonomy

...  Oligonucleotide signature sequences are short conserved sequences specific for a phylogenetically defined group of organisms  either complete or, more often, specific rRNA fragments can be compared  when comparing rRNA sequences between 2 organisms, their relatedness is represented by an associa ...
BLAST Database Searching
BLAST Database Searching

... if BlastP is more Sensitive? •  Non-translated sequences (RNA genes, promotors, etc) •  Closely related species, where you expect sequence identity > 70% ...
E. coli
E. coli

... binding of ribosomes to the RNA maintains transcript stability and promotes efficient translation. This bacterial translation system gives efficient expression of either prokaryotic or eukaryotic gene products in a short amount of time. For the highest protein yield and the best initiation fidelity, ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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