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DNA damage, repair and recombination
DNA damage, repair and recombination

... incorporated. A second round of replication fixes the mutation permanently in the DNA. ...
Western Blots. After toxin treatment, non-adherent
Western Blots. After toxin treatment, non-adherent

... phosphoribosyltransferase I (HPRT1). The data collected from these quantitative PCRs defined a threshold cycle number (Ct) of detection for the target or housekeeping genes in each cDNA sample. The relative quantity (RQ) of target, normalized to geometric means of the housekeeping genes and relative ...
7.014 Solution Set 4
7.014 Solution Set 4

... On the figure above, identify each complex. For the bacteria-human hybrid, indicate which strand is bacterial, and which is human. Briefly justify your choices. Human DNA for the same protein is likely to be significantly longer than a bacterial gene encoding homologous bacterial protein because the ...
The role of dSAGA specific histone acetylation in regulation of gene
The role of dSAGA specific histone acetylation in regulation of gene

... shared parts harbor a zinc finger-like ZZ and a SANT domain, characteristic for all known ADA2 proteins. The isoforms differ in their C-terminal regions, which ...
Chapter 10 Structure and Function of DNA
Chapter 10 Structure and Function of DNA

... Mutations may result from:  Errors in DNA replication  Physical or chemical agents called mutagens ...
P 1 - Faperta UGM
P 1 - Faperta UGM

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Hemophilia - Genomics Help
Hemophilia - Genomics Help

... “Promoter/Upstream by 1000 bases” and uncheck the box for “Introns” (this will remove all introns from the sequence that is retrieved). We also want to add 500 bases past the end of the gene, so check the box for “Downstream by 100 bases.” Also, make sure that under “Sequence Formatting Options,” th ...
II. Changes in chromosome number
II. Changes in chromosome number

... rearrangements are subject to natural selection and thus serve as instruments of evolution. a. Deletions remove DNA from a chromosome. Homozygosity for a large deletion is usually lethal, but even heterozygosity for a large deletion can create a deleterious genetic imbalance. Deletions may uncover r ...
Translation - Olympic High School
Translation - Olympic High School

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The Path From Genes to Proteins
The Path From Genes to Proteins

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PCR: Basics & Miniturization
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Solutions for Practice Problems for Molecular Biology, Session 5
Solutions for Practice Problems for Molecular Biology, Session 5

... technique in bacteria, and all added genes and regulatory regions can be expected to act as if they were a part of the genome.) This does not rescue the mutant phenotype observed in mutant 8; that is, these bacteria are still constitutive. Does this additional information allow you to narrow your op ...
Genetics of bacteria
Genetics of bacteria

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Abstract
Abstract

... molecular ecology. We initiated shipboard enrichments by inoculating anaerobic culture medium with samples collected during two expeditions onboard of the R/V Atlantis/Alvin (during the Summer 2006 and in January 2007) on the East Pacific Rise (EPR), at 9°50’N, 104°17’W. Currently, we have isolated ...
Little Else But Parasites
Little Else But Parasites

... may be screened simultaneously, for example, with RNA expression microarrays that can be used to predict a prognosis of cancer (9). Or the entire proteome of serum may be scanned by mass spectroscopy to discriminate among persons with and without cancer (7). Products of discovery-based research incl ...
Genome sequence and gene compaction of the eukaryote parasite
Genome sequence and gene compaction of the eukaryote parasite

... 51.0%). The 1,997 protein-coding DNA sequences (CDSs) represent about 90% of the chromosome cores, as a result of generally short intergenic regions (see Supplementary Information). Gene density is slightly lower than that observed in the nucleomorph genome of the cryptomonad Guillardia theta13. Onl ...
Genetics of Viruses and Bacteria
Genetics of Viruses and Bacteria

... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
gida bi̇yoteknoloji̇si̇-2
gida bi̇yoteknoloji̇si̇-2

...  Eucaryotes have 3 RNA polmerases (l, ll, lll) all of which involve in the trbscription of different genes. ...
Name three amino acids that are typically found at the
Name three amino acids that are typically found at the

... Q24 (1 point). Which of the options a-c apply for a eukaryotic organism? Explain your reasoning. d) The number of different proteins exceeds the number of different protein coding genes. e) The number of different protein coding genes exceeds the number of different proteins. f) There is an equal n ...
ChromoWheel: a new spin on eukaryotic chromosome visualization
ChromoWheel: a new spin on eukaryotic chromosome visualization

... with version 6.1 of Netscape or higher. We expect SVG to be supported more generally in the near future by all popular browsers. The exact behaviour of, e.g. zooming is dependent on the browser plug-in. Chromosomes are represented as thick coloured arcs along the circle edge. Across these arcs, line ...
Agro bacterium-mediated Transformation
Agro bacterium-mediated Transformation

... called the Ti plasmid, which is found within the bacterium Agrobacterium tumefaciens. Fraley et al. (1983) and An et al. (1985) exploited the natural ability of Agrobacterium tumefaciens to transfer DNA into plant chromosomes. This gram-negative, rod-shaped, motile bacterium lives in soil and invade ...
Genetics of Viruses and Bacteria
Genetics of Viruses and Bacteria

... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
Before you begin this in-class project, you will need the following
Before you begin this in-class project, you will need the following

... Before the mRNA exits the nucleus, 3 post-transcriptional modifications occur: 1. Introns (intragenic sequences) are spliced out of the mRNA 2. On the 5’ end of the mRNA, a 5’-methyl-guanosine cap is added 3. On the 3’ end of the mRNA, a poly-A tail is added mRNAs are exported out of the nucleus and ...
File
File

... DNA codes for Proteins (and sometimes RNA) • The sequence of _______________________________ in DNA codes for proteins!!!! – Order of ____________________ ______________________ – Central to cell function and life • Tells the cell what to do, what to produce, and when to do it!!! DNA Between Organis ...
RNA - Mr. Dudley's Website
RNA - Mr. Dudley's Website

... Divided into 23 pairs of chromosomes. ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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