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DNA replication notes
DNA replication notes

... Primase insert the appropriate base pairs on each side of the template strand, starting at specific spots, then DNA polymerase extends the nucleotide sequence all the way down the strand. Ligase “proofreads” the bases inserted to make sure they were paired correctly and fills in gaps in the phosphat ...
Ch. 17 From Gene to Protein
Ch. 17 From Gene to Protein

... has three binding sites for tRNA  The P site ...
DNA RNA
DNA RNA

... So what is a telomere anyway? • A telomere is a repeating DNA sequence (for example, TTAGGG) at the end of the body's chromosomes. • The telomere can reach a length of 15,000 base pairs. • Telomeres function by preventing chromosomes from losing base pair sequences at their ends. They also stop chr ...
Employing Cell-free DNA from Maternal Plasma for
Employing Cell-free DNA from Maternal Plasma for

... These alleles include the ones used in the detection of the rhesus D gene (RhD) in rhesus negative mothers, and fetal sex determination, using sequences from the Y chromosome. For example, DYS14 (a sequence located on the testis-specific Y encoded protein 1 (TSPY) gene, or the SRY (sex-determining r ...
Systems Biology Conceptual Modeling by Means of Discrete
Systems Biology Conceptual Modeling by Means of Discrete

... copies of the genes to be expressed are transcribed from the DNA in the form of messenger RNA (mRNA) molecules. The mRNAs are used by the protein-synthesizing machinery of the cell to make the appropriate proteins. This process, which takes place on sub-cellular particles called ribosome, is referre ...
A Superfamily of Proteins with Novel Cysteine
A Superfamily of Proteins with Novel Cysteine

... RLKs and share limited sequence homology among each other. However, all these RLK proteins contain two copies of the C-X8-C-X2-C motif in their extracellular domains (Fig. 1). A fourth Cys residue is usually also found at the C-terminal side of the C-X8C-X2-C motif but its position varies slightly a ...
Document
Document

... environment as needed (not continuously). Constitutive genes Continuously expressed. ...
DNA replication
DNA replication

... plasmids varies from 1 to over 400 kilobase pairs (kbp). There may be one copy, for large plasmids, to hundreds of copies of the same plasmid in a single cell, or even thousands of copies, for certain artificial plasmids selected for high copy number. Plasmids can be part of the mobilome, since they ...
synthetic gene networks that count
synthetic gene networks that count

... bioremediation, or medical purposes) • Mul-indu-DIC : sequential events (in settings: developmental biology and gene cascades • sin-indu- DIC : events encountered in its environment (e.g., for biosensing); • SIMM design :in synthetic circuits to maintain genetic memory of low-frequency events ...
PCR
PCR

... • Restriction fragment analysis is sensitive enough to distinguish between two alleles of a gene that differ by only base pair in a restriction site. ...
Bacterial Gene Regulation
Bacterial Gene Regulation

... • Depends on whether or not the ribosome is stalled in region 1 • If tryptophan is in short supply, ribosome has difficulty filling the need for two sequential tryptophans during translation • Ribosome stalls at 1, allowing 2 to interact with 3 • 2-3 antitermination loop forms, allowing transcriptio ...
chapter 17 from gene to protein
chapter 17 from gene to protein

... The most remarkable stage of RNA processing occurs during the removal of a large portion of the RNA molecule in a cut-and-paste job of RNA splicing. ...
Gene Expression in Prokaryotes
Gene Expression in Prokaryotes

... genes together so that they can be regulated together. This grouping is called an operon. The clustered genes are transcribed together from one promoter giving a polycistronic messenger. ...
Protein Synthesis - Austin Community College
Protein Synthesis - Austin Community College

... The most remarkable stage of RNA processing occurs during the removal of a large portion of the RNA molecule in a cut-and-paste job of RNA splicing. ...
Modeling and Associated Visualization Needs
Modeling and Associated Visualization Needs

... Statistical genetic methods II • Why does it work? – Because there are sufficient regimes of near linearity buried in mechanistic network eq’ns that general linear statistical models have levels of predictive skill useful for some purposes (e.g. crop breeding) – Rest assured that there are limits t ...
Differential gene expression in ES/PNET with type 1 vs type 2 EWS
Differential gene expression in ES/PNET with type 1 vs type 2 EWS

... Translocation-associated sarcomas 1. General biological features and comparison to sarcomas with non-specific cytogenetic alterations 2. Insights from microarray-based expression profiling of translocation-associated sarcomas ...
RNA DNA
RNA DNA

... An exciting revelation of the Human Genome Project is how similar all human beings are on the genetic level we are all 99.9% the same! This means that a comparison of the DNA sequence of two individuals would reveal approximately one different nucleotide for every thousand nucleotides of sequence. S ...
PCR applications in diagnosis of parasitic diseases
PCR applications in diagnosis of parasitic diseases

...  RT_PCR quantitative measurement of RNA or DNA:  there will be detection of “amplification associated florescence” in each cycle. It is used in diagnosis of many conditions other than parasitological infections. ...
Searching for Genes
Searching for Genes

... What you just did in Table 3 is referred to as “reverse transcription.” This is actually what some RNA viruses do when they infect cells. They reverse-transcribe their RNA and incorporate it into the DNA of the host cell. This process is also used by molecular biologists (biologists whose studies in ...
p 1.) What is a sigma factor? Why does the cell contain multiple
p 1.) What is a sigma factor? Why does the cell contain multiple

... Is a subunit of the RNA polymerase that recognizes the promoter region on the DNA and is required to RNA initiation. It is thought be released from the RNA polymerase holoenzyme after initiation has occurred. Different sigma factors recognize different promoter regions and help to regulate the trans ...
Unit 5 quesitons
Unit 5 quesitons

... 52. What is a stem cell and why is it important? 53. Briefly describe the levels of DNA packing in eukaryotic cells. 54. What are histones? 55. Describe a possible use for repetitive sequences. 56. Describe and give an example of a multigene family. 57. Draw a diagram of a typical eukaryotic gene an ...
Mutation
Mutation

... Types of Gene Mutations • Frameshift Mutation – involves the insertion or deletion of a nucleotide in the DNA sequence. – This usually has greater effects because a frameshift mutation shifts the entire sequence of base pairs which follow it. – An example of how it works: • You start with the sequ ...
Slide 1
Slide 1

... 2. Translation – the mRNA, with the help of the ribosome, forms a chain of amino acids (eventually forming a protein) based on the information contained on the mRNA. ...
DNA Challenge DNA Challenge
DNA Challenge DNA Challenge

... DNA and RNA differ. ...
Mutation of a Ubiquitously Expressed Mouse Transmembrane
Mutation of a Ubiquitously Expressed Mouse Transmembrane

... a cDNA corresponding to exon 1, part of exon 4, and a portion of intron 4, in which it terminates. This cDNA was reported to encode the human cytomegalovirus (HCMV) gH receptor (Baldwin et al. 2000). A BLAST search of the mouse TAPT1 amino acid sequence against human proteins revealed a high similar ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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