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Protein Synthesis, Processing, and Regulation
Protein Synthesis, Processing, and Regulation

... joined to tRNA during the second step of the reaction. Recognition of the correct tRNA by the aminoacyl tRNA synthetase is also highly selective; the synthetase recognizes specific nucleotide sequences (in most cases including the anticodon) that uniquely identify each species of tRNA. After being a ...
Structure-Based Prediction of DNA Target Sites by Regulatory Proteins
Structure-Based Prediction of DNA Target Sites by Regulatory Proteins

... These results show that target binding sites for several regulatory proteins are successfully predicted, and our data suggest that this method can serve as a powerful tool for predicting multiple target sites and target genes for regulatory proteins. Proteins 1999;35:114–131. r 1999 Wiley-Liss, Inc. ...
Chapter 10 - People Server at UNCW
Chapter 10 - People Server at UNCW

... Copyright © McGraw-Hill Education. Permission required for reproduction or display. ...
Chapter 16 The Molecular Basis of Inheritance
Chapter 16 The Molecular Basis of Inheritance

... – Each of the two new daughter molecules will have one old strand, derived from the parent molecule, and one newly made strand Parent cell ...
DNA Replication and Telomere Maintenance
DNA Replication and Telomere Maintenance

... Leading strand synthesis is continuous • Discontinuous replication occurs on the 5′→3′ template strand (lagging strand). • DNA is copied in short segments called “Okazaki fragments” moving in the opposite direction to the replication fork. ...
Tuning Biphenyl Dioxygenase for Extended Substrate Specificity
Tuning Biphenyl Dioxygenase for Extended Substrate Specificity

... 1986; Bopp, 1986). Due to its broad substrate specificity, it was used as the starting template for generating variant enzymes that can act on an extended set of PCB congeners. Because substrate specificity is determined mainly by the bphA region, only this gene fragment was subjected to directed ev ...
Molecular insights into mitochondrial transcription and its
Molecular insights into mitochondrial transcription and its

... triphosphate (ATP), through the process of oxidative phosphorylation. The mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochon ...
Landick R, Yanofsky C. 1987. Transcription
Landick R, Yanofsky C. 1987. Transcription

... attenuator. This decision is based on the cellular level of charged tRNA Trp. Under starvation conditions, in which all tRNATrp essentially is uncharged, the rate of read through transcription at the attenuator increases about eightfold over the rate observed when tRNATrp is fully charged. We do not ...
Gene Section TYR (tyrosinase (oculocutaneous albinism IA))  Atlas of Genetics and Cytogenetics
Gene Section TYR (tyrosinase (oculocutaneous albinism IA)) Atlas of Genetics and Cytogenetics

... probability of metastasis. This neoplasm is greatly resistant to most conventional therapies. Due to the longevity of melanocytes, these cells are considered to have a greater mutagenic burden. This burden is also greater due to the position of melanocytes within the skin and their exposure to UV li ...
here
here

... gene sets based on genomic locations, merging of genetic data from specific organisms ...
electron microscopic autoradiographic study of rna synthesis in
electron microscopic autoradiographic study of rna synthesis in

... that in yeast both speciesof ribosomal RNA derive from a common large precursor molecule. Hence it is clear that yeast, one of the most primitive eukaryotes, possessesa mechanism for the processing of ribosomal RNA which resemblesthat of higher eukaryotes. This raises the question whether, in yeast, ...
CRISPR/Cas9: Tools and Applications for Eukaryotic Genome Editing
CRISPR/Cas9: Tools and Applications for Eukaryotic Genome Editing

... Cas9 as a double nickase. If you situate two units of Cas9 nickase on opposite strands of DNA, then a nick plus another nick equals a double-stranded break. This works efficiently and it works across a wide number of distances. Double nicking can happen as far as 100 nucleotides away from each other ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA

... containing 50% glycerol. Reaction volumes must be kept to a minimum by reducing the amount of water in the reaction as much as possible. However, the restriction enzyme must contribute less than 0.1 volume of the final reaction mixture; otherwise, the enzyme activity may be inhibited by glycerol. Di ...
HEMOGLOBINOPATHY412 KB
HEMOGLOBINOPATHY412 KB

... HEMOGLOBINOPATHY • Mutations in the genes that encode the α or β subunits of Hb potentially can affect its biological function • More than 800 known mutant human Hbs are both extremely rare and benign, with no clinical abnormalities • When a mutation compromises bilogic function hemoglobinopathy ...
A green glow
A green glow

... of tumours in laboratory animals. In the same way, “labelling” neurons with GFP in young mice shows both their migration and their evolution in the brain, thus giving an indication on cerebral development. GFP is also used to visualize something even smaller than cells: proteins. Several techniques ...
Poster
Poster

... injured spines complex and nearly impossible. This is even more drastic when we take into account that the spine is a part of the central nervous system, controlled directly from the motor cortex in the brain, and if it does not function properly, there can be major repercussions in terms of movemen ...
Whey Protein Concentrate
Whey Protein Concentrate

Concept 3.1 Nucleic Acids Are Informational
Concept 3.1 Nucleic Acids Are Informational

... cannot be created or destroyed (energy of the universe is constant) • 2nd: every energy transfer of transformation makes the universe more disordered (every process increases the entropy of the universe) • Entropy: quantitative measure of disorder that is proportional to randomness (designated by th ...
Identification of the nuclear localization signals within the Epstein
Identification of the nuclear localization signals within the Epstein

Novel regulatory roles of cAMP receptor proteins in
Novel regulatory roles of cAMP receptor proteins in

... Mycobacterium smegmatis is a fast-growing, saprophytic, mycobacterial species that contains two cAMP-receptor protein (CRP) homologues designated herein as Crp1 and Crp2. Phylogenetic analysis suggests that Crp1 (Msmeg_0539) is uniquely present in fast-growing environmental mycobacteria, whereas Crp ...
Topic 1: Statistical analysis (2 hours)
Topic 1: Statistical analysis (2 hours)

... because they are larger than a typical cell / carry out all life functions; some tissues / organs contain large amounts of extracellular material; eg vitreous humour of eye / mineral deposits in bone / xylem in trees / other example; statement of cell theory / all living things / most tissues are co ...
Pvlea-18, a Member of a New Late-Embryogenesis
Pvlea-18, a Member of a New Late-Embryogenesis

... review, see Dure, 1993a; Ingram and Bartles, 1996). Other hypothetical roles assigned to the LEA proteins are sequestration of ions (Dure, 1993b), molecular chaperone activity (Close, 1996), and transport of nuclear-targeted proteins during stress (Goday et al., 1994). Based on regions of significan ...
DNA-Catalyzed Covalent Modification of Amino Acid Side Chains in
DNA-Catalyzed Covalent Modification of Amino Acid Side Chains in

... (deoxyribozymes) that modify side chains of peptide substrates, with the long-term goal of achieving DNA-catalyzed covalent protein modification. We recently described several deoxyribozymes that modify tyrosine (Tyr) or serine (Ser) side chains by catalyzing their reaction with 50 -triphosphorylated ...
Anterior neurectoderm is progressively induced
Anterior neurectoderm is progressively induced

... has been suggested that diffusible signals can pass in a planar fashion from the mesoderm to the ectoderm in vivo. Currently the molecules involved in planar and/or vertical signalling are unknown. However, two candidates have been identified which have neural-inducing activity in vitro and are expr ...
Table S6: Domains present in the primary network generated from
Table S6: Domains present in the primary network generated from

... typically between 205 to 258 amino acids in length. This family of proteins are functionally uncharacterised. This protein is found in bacteria and eukaryotes. Proteins in this family are about 220 amino acids in length. This family of proteins are functionally uncharacterised. This protein is found ...
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Silencer (genetics)

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