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Mutations PPT (Day 2)
Mutations PPT (Day 2)

... 1. Polar Bears are known to live in the Arctic. These bears have thicker fur than bears that live in warmer climates. Would the mutation in the bears genes that results in thicker fur be beneficial, harmful, or neither? Why? (Think about the climate in the Arctic.) 2. An albino (white) snake lives i ...
protein synthesis fill-in
protein synthesis fill-in

... DNA Begins the Process • ______ is found inside the _______ • _________, however, are made in the _________ of cells by organelles called ribosomes • Ribosomes may be free in the cytosol or attached to the _______________________ copyright cmassengale ...
Gene expression - McGraw Hill Higher Education
Gene expression - McGraw Hill Higher Education

... Regulation of gene expression in eukaryotes (cont.) • Eukaryotic promoters do not provide sufficient recognition signals for RNA polymerase II to initiate transcription in vivo • Core promoter elements must each be recognised by regulatory proteins (basal factors and coactivators) which bind to the ...
Epigenetic Modifications - Carol Lee Lab
Epigenetic Modifications - Carol Lee Lab

... -- Paramutation: where interaction between two alleles at a single locus, results in a heritable change in expression of one allele that is induced by the other allele. Mechanism is not fully understood, but could occur via methylation or regulatory RNAs. Paramutation violates Mendel’s first law, wh ...
Epigenetic Inheritance - Carol Eunmi LEE
Epigenetic Inheritance - Carol Eunmi LEE

... -- Genomic imprinting: where methylation and histone modifications alter gene expression without altering the genetic sequence. When inherited, these “epigenetic marks” are established in the germline and are maintained throughout all somatic cells of an organism. -- Gene Silencing: could occur ...
T-DNA Mutagenesis
T-DNA Mutagenesis

... Agrobacteria tumefaciens is a bacteria found on certain plants that were found to cause tumors on wounded plant areas. Found to contain Ti (Tumor inducing) plasmid that creates a mutation in the plants genomic sequence. The Ti plasmid’s ability to integrate itself into a DNA sequence was isolated an ...
Brooker Chapter 18 - Volunteer State Community College
Brooker Chapter 18 - Volunteer State Community College

... vectors used to clone small segments of DNA Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Plasmid
Plasmid

...  can replicate independently  found mostly in bacterial cells  carry genes that benefit the survival of the organism (antibiotic resistance). ...
13. DNA Replication
13. DNA Replication

... 1. Review of DNA structure DNA double helix model:  DNA made of nucleotide building blocks linked into polymer chains  Bases are on inside, sugars and phosphates form a backbone on outside  Two strands exist in an antiparallel arrangement ...
AtPTB-like 1 negatively regulates splicing inclusion of a plant
AtPTB-like 1 negatively regulates splicing inclusion of a plant

... showing 64.1% global peptide sequence identity and shared 7 introns in conserved positions. ...
DNA and protein synthesis
DNA and protein synthesis

... cells are one such exception.) In prokaryotic cells there may be just one DNA molecule. In eukaryotic cells there are usually several. For example, humans have 46 DNA molecules in their cells (when they are not dividing), because each of our 46 chromosomes contains one DNA molecule. The DNA molecule ...
Document
Document

... (Clark and Kao, 1991). Last, the S locus of Lycopersicon peruvianum has been shown by genetic mapping to be located close to the centromere of chromosome I (Bernatzky, 1993), and the S locus of Petunia hybrida has been shown by fluorescence in situ hybridization to be located close to the centromere ...
RESTRICTION ENDONUCLEASES
RESTRICTION ENDONUCLEASES

... groups (the phosphodiester linkage). This results in phosphate groups on the 5’ ends and hydroxyl groups on the 3’ ends of both strands. The biological function of restriction enzymes is to protect the bacterial cell against the introduction of foreign DNA into the cell (Turner et al., 1997). DNA me ...
molecular_gene_cloning_restriction
molecular_gene_cloning_restriction

... groups (the phosphodiester linkage). This results in phosphate groups on the 5’ ends and hydroxyl groups on the 3’ ends of both strands. The biological function of restriction enzymes is to protect the bacterial cell against the introduction of foreign DNA into the cell (Turner et al., 1997). DNA me ...
M0290Datasheet-Lot0601204
M0290Datasheet-Lot0601204

... DNA, RNA and ribo- and deoxyribonucleoside triphosphates. Since CIP-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate (1). This property can be used to decrease the vector background in cloning strategies. Source: Calf intestinal mucosa Molecular Weight: 6 ...
DNA / RNA blue print of life PPT
DNA / RNA blue print of life PPT

... Assembling Proteins from the DNA Instructions ...
The wrong file for Lecture 8 was posted on the website. I`ve sent the
The wrong file for Lecture 8 was posted on the website. I`ve sent the

... (VNTPs). The sequences are variable in length (10-100 bp), but within a repeat sequence, the individual sequences will be the same. VNTPs create regions of 1000-5000 bp in length ...
Datasheet for Alkaline Phosphatase, Calf Intestinal (CIP)
Datasheet for Alkaline Phosphatase, Calf Intestinal (CIP)

... DNA, RNA and ribo- and deoxyribonucleoside triphosphates. Since CIP-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate (1). This property can be used to decrease the vector background in cloning strategies. Source: Calf intestinal mucosa Molecular Weight: 6 ...
video slide - Independent School District 196
video slide - Independent School District 196

... ribosomal subunit 1 A small ribosomal subunit binds to a molecule of mRNA. In a prokaryotic cell, the mRNA binding site on this subunit recognizes a specific nucleotide sequence on the mRNA just upstream of the start codon. An initiator tRNA, with the anticodon UAC, base-pairs with the start codon, ...
Document
Document

Organic Molecules Worksheet: Review
Organic Molecules Worksheet: Review

... (outside) of the DNA ladder are made from alternating sugars, called deoxyribose, and phosphates (sugar-phosphate-sugar-phosphate…). The rungs (inside) of the ladder are made of four different kinds of nitrogen containing bases, with one base hanging off of the sugar portion of each rail. The four n ...
PITT pGLO Transformation Lab Protocol
PITT pGLO Transformation Lab Protocol

... Internal membrane-enclosed organelles, including a nucleus ...
UNIT (12) MOLECULES OF LIFE
UNIT (12) MOLECULES OF LIFE

... ladder fixed at one end to the top of a pole, and subsequently wound downward around it without twisting the ladder. The two polynucleotide strands are connected by hydrogen bonds formed between a purine on one strand and a pyrimidine on the other. In DNA, adenine is always paired with thymine and g ...
Transcription Translation PowerPoint
Transcription Translation PowerPoint

... 1. Cutting out certain ____ during processing gives tRNA its ____ shape. 2. The anticodon on tRNA is complimentary to the ____ on mRNA. 3. Why does tRNA have an amino acid binding site? (What is tRNA’s function?) ...
Document
Document

... a Two-stage Freezing Process, and Revival of Culture • During equilibration, an aliquot of 0.5 to 1.0 ml of the cell suspension is dispensed into each plastic cryovial or glass ampoule. • They are tightly closed, clamped onto labeled aluminum canes, and placed at -30°C for about 1 h or for a few min ...
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Silencer (genetics)

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