A single mutation in the core domain of the lac repressor reduces
... Background: The lac operon provides cells with the ability to switch from glucose to lactose metabolism precisely when necessary. This metabolic switch is mediated by the lac repressor (LacI), which in the absence of lactose binds to the operator DNA sequence to inhibit transcription. Allosteric rea ...
... Background: The lac operon provides cells with the ability to switch from glucose to lactose metabolism precisely when necessary. This metabolic switch is mediated by the lac repressor (LacI), which in the absence of lactose binds to the operator DNA sequence to inhibit transcription. Allosteric rea ...
RNA interference and its role in the regulation of eucaryotic gene
... eukaryotic organisms. RNAi is also capable of silencing transposons in germ line cells and fighting RNA virus infection. Enzymes involved in this process exhibit high homology across species. Some of these enzymes are involved in other cellular processes, for instance developmental timing, suggestin ...
... eukaryotic organisms. RNAi is also capable of silencing transposons in germ line cells and fighting RNA virus infection. Enzymes involved in this process exhibit high homology across species. Some of these enzymes are involved in other cellular processes, for instance developmental timing, suggestin ...
Text Book of Molecular Biology
... P11 the middle fig Nucleotides: A nucleotide is a nucleoside with one or more phosphate groups bound covalently to the 3’-，5’- or (in ribonucleoside only，the 2’-position) by phosphoester bond. ...
... P11 the middle fig Nucleotides: A nucleotide is a nucleoside with one or more phosphate groups bound covalently to the 3’-，5’- or (in ribonucleoside only，the 2’-position) by phosphoester bond. ...
Recombinant DNA - Fulton County Schools
... – How do scientists copy the DNA of living organisms? – The first step in using the polymerase chain reaction method to copy a gene is to heat a piece of DNA, which separates its two strands. Then, as the DNA cools, primers bind to the single strands. Next, DNA polymerase starts copying the region b ...
... – How do scientists copy the DNA of living organisms? – The first step in using the polymerase chain reaction method to copy a gene is to heat a piece of DNA, which separates its two strands. Then, as the DNA cools, primers bind to the single strands. Next, DNA polymerase starts copying the region b ...
Bacterial Transformation with Recombinant DNA
... containing the antibiotic. This makes it possible to isolate bacteria that contain the plasmid. Plasmids also contain restriction enzyme sites in specific locations. These sites make it possible to open the circular plasmid and insert foreign DNA. In addition, many plasmids also have differential ma ...
... containing the antibiotic. This makes it possible to isolate bacteria that contain the plasmid. Plasmids also contain restriction enzyme sites in specific locations. These sites make it possible to open the circular plasmid and insert foreign DNA. In addition, many plasmids also have differential ma ...
Open Reading Frame (ORF) finding - Manatee
... – binding sites – catalytic sites – full length match with significant identity between amino acids (>35% minimum) ...
... – binding sites – catalytic sites – full length match with significant identity between amino acids (>35% minimum) ...
Mycorrhiza
... tissues at different developmental stages and under varying environmental conditions (for a review, see Büttner and Sauer 2000; Lemoine 2000; Williams et al. 2000). These transporters are members of the major facilitator superfamily, which is characterised by a common structural motif consisting of ...
... tissues at different developmental stages and under varying environmental conditions (for a review, see Büttner and Sauer 2000; Lemoine 2000; Williams et al. 2000). These transporters are members of the major facilitator superfamily, which is characterised by a common structural motif consisting of ...
清华大学本科生考试试题专用纸
... not only are unable to use glucose properly but also fail to synthesize fatty acids from carbohydrates or amino acids. Untreated diabetics have increased rate of fat oxidation and ketone body formation, as a consequence, lose weight. 43. Suppose that a bacterial mutant is found to replicate its DNA ...
... not only are unable to use glucose properly but also fail to synthesize fatty acids from carbohydrates or amino acids. Untreated diabetics have increased rate of fat oxidation and ketone body formation, as a consequence, lose weight. 43. Suppose that a bacterial mutant is found to replicate its DNA ...
1. Sequence analysis
... Alignment of genomic sequence with mRNA. Retrieve the nt sequences em:V00594 (Human mRNA for metallothionein) and em:J00271 (corresponding genomic sequence). (Use the database browser). Compare these sequences by doing an alignment with GAP. Based on the alignment, how many exons are there in this g ...
... Alignment of genomic sequence with mRNA. Retrieve the nt sequences em:V00594 (Human mRNA for metallothionein) and em:J00271 (corresponding genomic sequence). (Use the database browser). Compare these sequences by doing an alignment with GAP. Based on the alignment, how many exons are there in this g ...
Protein degradation and regulation
... environmental toxins, translation errors and genetic mutations can damage proteins. Misfolded proteins are highly deleterious to the cell because they can form non-physiological interactions with other proteins. Repair proteins called chaperones can, in many instances, restore the native conformatio ...
... environmental toxins, translation errors and genetic mutations can damage proteins. Misfolded proteins are highly deleterious to the cell because they can form non-physiological interactions with other proteins. Repair proteins called chaperones can, in many instances, restore the native conformatio ...
Cloning and characterization of the Xenopus laevis p8 gene
... Conserved amino acids are highlighted. A putative basic helix-loop-helix (bHLH) region is illustrated under the sequence. The region defined by arrows indicates a nuclear targeting sequence (Mallo et al. 1997). (b) Full-length amino acid sequence identity between the four species. (c) Sequence ident ...
... Conserved amino acids are highlighted. A putative basic helix-loop-helix (bHLH) region is illustrated under the sequence. The region defined by arrows indicates a nuclear targeting sequence (Mallo et al. 1997). (b) Full-length amino acid sequence identity between the four species. (c) Sequence ident ...
Catalog# 786-842 PROTOCOL - G
... through amide bonds. The coupling chemistry used generates a highly stable purification resin that is stable most commonly used buffers and denaturants. Heparin is a linear glycosaminoglycan composed of equimolar quantites of glucosamine and glucuronic acid, alternatively linked by α(1→4) glycosidic ...
... through amide bonds. The coupling chemistry used generates a highly stable purification resin that is stable most commonly used buffers and denaturants. Heparin is a linear glycosaminoglycan composed of equimolar quantites of glucosamine and glucuronic acid, alternatively linked by α(1→4) glycosidic ...
Word copy
... Biological focus on higher eukaryote gene regulation and chromatin structure from atomic interactions to organelle (nucleus). Molecular structure analysis, especially proteins, DNA and protein-DNA interactions. Molecular modelling. Sequence analysis, especially protein sequences, protein binding sit ...
... Biological focus on higher eukaryote gene regulation and chromatin structure from atomic interactions to organelle (nucleus). Molecular structure analysis, especially proteins, DNA and protein-DNA interactions. Molecular modelling. Sequence analysis, especially protein sequences, protein binding sit ...
The Scientist : Lab Tools: Close Encounters
... The problem: Y2H measures only binary interactions, not multiprotein complexes. But coIP /MS requires high-quality, specific antibodies, few of which are available. Superti-Furga needed a generic way to isolate intact complexes from cells. The Solution: Superti-Furga and his team adapted a process c ...
... The problem: Y2H measures only binary interactions, not multiprotein complexes. But coIP /MS requires high-quality, specific antibodies, few of which are available. Superti-Furga needed a generic way to isolate intact complexes from cells. The Solution: Superti-Furga and his team adapted a process c ...
Chapter 5
... Answer: By using a probe specific for the DNA of interest, the clone can be identified. The probe is designed to hybridize to the DNA of the clone that has been transferred to a membrane. The probe is labeled with radioactivity or another tag so that it can be easily detected and the proper clone id ...
... Answer: By using a probe specific for the DNA of interest, the clone can be identified. The probe is designed to hybridize to the DNA of the clone that has been transferred to a membrane. The probe is labeled with radioactivity or another tag so that it can be easily detected and the proper clone id ...
Fastest, Easiest Adenoviral System Ever
... Adenoviral gene transfer is one of the most reliable methods for introducing genes into mammalian cells. Because infection by adenovirus is not cell-cycle dependent, you can deliver your gene to primary as well as transformed cell lines. Adenoviruses are ideal tools for protein production in mammali ...
... Adenoviral gene transfer is one of the most reliable methods for introducing genes into mammalian cells. Because infection by adenovirus is not cell-cycle dependent, you can deliver your gene to primary as well as transformed cell lines. Adenoviruses are ideal tools for protein production in mammali ...
ACT - Genetic Mutations-S
... 14. The amino acids produced from sequence 1 and sequence 2 in Model 1 do not have ellipses on the end of them, however the amino acids produced from sequence 3 and sequence 4 do have ellipses. Propose an explanation for the use or absence of the ellipses on these sequences. ...
... 14. The amino acids produced from sequence 1 and sequence 2 in Model 1 do not have ellipses on the end of them, however the amino acids produced from sequence 3 and sequence 4 do have ellipses. Propose an explanation for the use or absence of the ellipses on these sequences. ...
Full Text
... AT3G08510 AT4G18010 AT3G02230 AT1G03520 AT4G33550 AT1G64740 AT3G48100 AT3G58610 AT1G69295 AT3G09390 AT3G48530 AT2G37110 AT4G26530 AT3G20500 AT5G05730 AT4G28300 AT5G41210 AT4G19230 AT2G19990 AT3G57470 AT3G09180 AT3G08580 AT1G02870 AT2G17980 AT2G43750 AT1G30970 AT5G67220 AT5G24810 AT1G50300 AT3G21090 ...
... AT3G08510 AT4G18010 AT3G02230 AT1G03520 AT4G33550 AT1G64740 AT3G48100 AT3G58610 AT1G69295 AT3G09390 AT3G48530 AT2G37110 AT4G26530 AT3G20500 AT5G05730 AT4G28300 AT5G41210 AT4G19230 AT2G19990 AT3G57470 AT3G09180 AT3G08580 AT1G02870 AT2G17980 AT2G43750 AT1G30970 AT5G67220 AT5G24810 AT1G50300 AT3G21090 ...