Supplementary Information (doc 68K)
... (TCM) AIM-V (Life Technologies) supplemented with 5 % human AB serum, 2 mM Lglutamine and 50 µg / ml gentamycine to a concentration of 1 x 106 / ml. For every peptide concentration to be tested T2 cells were pulsed with a serial dilution of 0.1 – 100 µM peptide and then incubated for 16 hours at 37 ...
... (TCM) AIM-V (Life Technologies) supplemented with 5 % human AB serum, 2 mM Lglutamine and 50 µg / ml gentamycine to a concentration of 1 x 106 / ml. For every peptide concentration to be tested T2 cells were pulsed with a serial dilution of 0.1 – 100 µM peptide and then incubated for 16 hours at 37 ...
Description
... cDNA, which is then amplified by PCR. RT-PCR is widely used in expression profiling, to determine the expression of a gene or to identify the sequence of an RNA transcript, including transcription start and termination sites. If the genomic DNA sequence of a gene is known, RTPCR can be used to map t ...
... cDNA, which is then amplified by PCR. RT-PCR is widely used in expression profiling, to determine the expression of a gene or to identify the sequence of an RNA transcript, including transcription start and termination sites. If the genomic DNA sequence of a gene is known, RTPCR can be used to map t ...
060506-Meeting
... NEED: To transfer genetic information from one bacteria to another MEANS: Bacterial Conjugation NEED: To specifically control who can read the message ...
... NEED: To transfer genetic information from one bacteria to another MEANS: Bacterial Conjugation NEED: To specifically control who can read the message ...
BISC-576 Practical Statistics and Bioinformatics Instructors:
... Secondary structure elements, structural alignment, and fold classifications: This lecture will introduce alpha-helices and beta-sheets and the Ramachandran plot as means of identifying secondary structure elements of proteins. In addition, the basic principles for structural alignment methods will ...
... Secondary structure elements, structural alignment, and fold classifications: This lecture will introduce alpha-helices and beta-sheets and the Ramachandran plot as means of identifying secondary structure elements of proteins. In addition, the basic principles for structural alignment methods will ...
CHAPTER 1 Aim
... of global potato production over the period from 1950 to 1999 has shown a stable production of 30 million tonnes per year. This is due to overall increasing yields, despite the area grown with potato decreasing stably at 92000 ha per year. On a global scale, 51% of potato area is in Europe. 35% in A ...
... of global potato production over the period from 1950 to 1999 has shown a stable production of 30 million tonnes per year. This is due to overall increasing yields, despite the area grown with potato decreasing stably at 92000 ha per year. On a global scale, 51% of potato area is in Europe. 35% in A ...
Marie Connelly - DeSales University
... -7Avery, MacLeod and McCarty injected mice with strands of R (rough, non-virulent) and S (smooth, virulent) Streptococci and observed the affects it had upon the mice. When heat killed S strain cells were injected along with R strain cells, the mice developed pneumonia and died because of the live, ...
... -7Avery, MacLeod and McCarty injected mice with strands of R (rough, non-virulent) and S (smooth, virulent) Streptococci and observed the affects it had upon the mice. When heat killed S strain cells were injected along with R strain cells, the mice developed pneumonia and died because of the live, ...
A mitochondrial specific stress response in mammalian cells
... organelles, such as the endoplasmic reticulum unfolded protein response. Here we report on the discovery and characterization of a mitochondrial stress response in mammalian cells. We ®nd that the accumulation of unfolded protein within the mitochondrial matrix results in the transcriptional upregul ...
... organelles, such as the endoplasmic reticulum unfolded protein response. Here we report on the discovery and characterization of a mitochondrial stress response in mammalian cells. We ®nd that the accumulation of unfolded protein within the mitochondrial matrix results in the transcriptional upregul ...
Anabaena - Oxford Academic
... chococcus sp. PCC 7942 exhibited similar dark respiratory activity, as measured by oxygen uptake, to that of the wild-type [21]. Thus cyanobacteria may employ an alternative respiratory pathway when the OPP is non-functional. The OPP is also thought to be largely responsible for the supply of reduct ...
... chococcus sp. PCC 7942 exhibited similar dark respiratory activity, as measured by oxygen uptake, to that of the wild-type [21]. Thus cyanobacteria may employ an alternative respiratory pathway when the OPP is non-functional. The OPP is also thought to be largely responsible for the supply of reduct ...
Classifying Biological Full-Text Articles for Multi
... These findings therefore demonstrate that dexamethasone (皮質類固醇) is a potent inducer of multidrug resistance-associated protein (多抗藥性 蛋白質) expression in rat hepatocytes (肝細胞) through a mechanism that seems not to involve the classical glucocorticoid receptor (糖皮質激素受體) pathway. 1. Dexamethasone STIMUL ...
... These findings therefore demonstrate that dexamethasone (皮質類固醇) is a potent inducer of multidrug resistance-associated protein (多抗藥性 蛋白質) expression in rat hepatocytes (肝細胞) through a mechanism that seems not to involve the classical glucocorticoid receptor (糖皮質激素受體) pathway. 1. Dexamethasone STIMUL ...
Information Retrieval Performance and Method
... in high recall—retrieving as many abstracts as the text mining system can reasonably handle—and precision was not an immediate concern until after we sifted through the retrieved AD abstract corpus for relevant drug information. Therefore, the choice of selecting up to 1000 PubMed abstracts per quer ...
... in high recall—retrieving as many abstracts as the text mining system can reasonably handle—and precision was not an immediate concern until after we sifted through the retrieved AD abstract corpus for relevant drug information. Therefore, the choice of selecting up to 1000 PubMed abstracts per quer ...
![Page 1 United States Patent [19] Anderson et al](http://s1.studyres.com/store/data/012959380_1-379e058c34073d27ea66ba9d94381771-300x300.png)
Page 1 United States Patent [19] Anderson et al
... CMV can transform cells and stimulate growth. Both human 50 clovir, ACV) and certain combinations of these drugs have and non-human cells can undergo transformation when been ineffective in controlling CMV infection. Based on incubated with CMV. Transformed cells contain CMV anti~ preclinical and cl ...
... CMV can transform cells and stimulate growth. Both human 50 clovir, ACV) and certain combinations of these drugs have and non-human cells can undergo transformation when been ineffective in controlling CMV infection. Based on incubated with CMV. Transformed cells contain CMV anti~ preclinical and cl ...
Sanger dideoxy sequencing - Midlands State University
... such changes. A compound will act as a proton concentration buffer if it limits changes in proton concentration by binding protons when the proton concentration of the solution increases and releasing bound protons when the proton concentration decreases (Eqn 1). ...
... such changes. A compound will act as a proton concentration buffer if it limits changes in proton concentration by binding protons when the proton concentration of the solution increases and releasing bound protons when the proton concentration decreases (Eqn 1). ...
CSE 181 Project guidelines - Computer Science and Engineering
... • Act to transfer short pieces of information to different parts of cell • Provide templates to synthesize into protein • May be involved in the regulation of gene expression • Made of 4 types of nucleotides • Proteins • Make up the cellular structure • large, complex molecules made up of 20 types o ...
... • Act to transfer short pieces of information to different parts of cell • Provide templates to synthesize into protein • May be involved in the regulation of gene expression • Made of 4 types of nucleotides • Proteins • Make up the cellular structure • large, complex molecules made up of 20 types o ...
Review of the reconstruction
... from NDP and ATP is due to a unique nucleoside diphosphate kinase, encoded by ndk, whose transcriptional regulation is unknown. The purified enzyme exhibits different affinity (KM) for each NDP, which leads to GTP and UTP synthesis prior to CTP synthesis [28]. The regulation of the synthesis of deox ...
... from NDP and ATP is due to a unique nucleoside diphosphate kinase, encoded by ndk, whose transcriptional regulation is unknown. The purified enzyme exhibits different affinity (KM) for each NDP, which leads to GTP and UTP synthesis prior to CTP synthesis [28]. The regulation of the synthesis of deox ...
MyTaq™ HS DNA Polymerase
... tomato are used for agricultural research and are a ready source of DNA without causing too much damage to the main plant. The use of ISOLATE II and MyTaq means that high quality DNA can be extracted from many leaves and then used in PCR (fig. 1). Rice is a staple food for over half of the world’s p ...
... tomato are used for agricultural research and are a ready source of DNA without causing too much damage to the main plant. The use of ISOLATE II and MyTaq means that high quality DNA can be extracted from many leaves and then used in PCR (fig. 1). Rice is a staple food for over half of the world’s p ...
Improved recovery of DNA from polyacrylamide gels after in situ
... Reagent at 60 jC for 30 min. The volume of the DNA solution was decreased from 600 to 20 Al using glassmilk elution according to the manufacturer’s instruction (Geneclean II, Bio101). DNA concentration by ethanol precipitation or using commercially available kits which use chaotropic agents such as ...
... Reagent at 60 jC for 30 min. The volume of the DNA solution was decreased from 600 to 20 Al using glassmilk elution according to the manufacturer’s instruction (Geneclean II, Bio101). DNA concentration by ethanol precipitation or using commercially available kits which use chaotropic agents such as ...
Poster
... infectious proteins are called prions and the diseases they cause have no known cure. Prions are responsible for transforming healthy brain proteins into prion replicas, therefore spreading the disease and disrupting normal functions. This transformation occurs when the mainly alpha helical form of ...
... infectious proteins are called prions and the diseases they cause have no known cure. Prions are responsible for transforming healthy brain proteins into prion replicas, therefore spreading the disease and disrupting normal functions. This transformation occurs when the mainly alpha helical form of ...
Detection of alien viruses and viroids in plants by siRNA
... Viruses are always detected by RNA silencing in infected plants! In virus-susceptible plants, however, RNA silencing cannot prevent infection. Jari Valkonen ...
... Viruses are always detected by RNA silencing in infected plants! In virus-susceptible plants, however, RNA silencing cannot prevent infection. Jari Valkonen ...
- Biological Sciences
... The mitochondrial genome of the platypus is 17,019 base pairs long and encodes 22 tRNAs, 13 proteins, and 2 rRNAs. As in other vertebrates, 12 of the proteins are encoded on the L-strand. In six of the ten cases where the stop codon TAA is used, the codons are inferred to be incomplete and are presu ...
... The mitochondrial genome of the platypus is 17,019 base pairs long and encodes 22 tRNAs, 13 proteins, and 2 rRNAs. As in other vertebrates, 12 of the proteins are encoded on the L-strand. In six of the ten cases where the stop codon TAA is used, the codons are inferred to be incomplete and are presu ...
Bacterial and Firefly Luciferase Genes in Transgenic Plants
... synthesis of an autoinducer, N-(P-ketocaproy1)-homoserine lactone. lux gene expression correlates with the density of cell cultures. Initially the luxR protein is constitutively produced and the luxCDABE operon is expressed only a t a low level. At higher cell densities an increased concentration of ...
... synthesis of an autoinducer, N-(P-ketocaproy1)-homoserine lactone. lux gene expression correlates with the density of cell cultures. Initially the luxR protein is constitutively produced and the luxCDABE operon is expressed only a t a low level. At higher cell densities an increased concentration of ...
LIN-28 co-transcriptionally binds primary let
... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
ONE
... candidates are the single group II intron in rpl2 and the second intron in rps12. The maturase MatK presumably helps fold the intron RNA into the catalytically-active structure. The 3’ end of the matK was identified to contain a conserved region of about 100 amino acids and this region was named dom ...
... candidates are the single group II intron in rpl2 and the second intron in rps12. The maturase MatK presumably helps fold the intron RNA into the catalytically-active structure. The 3’ end of the matK was identified to contain a conserved region of about 100 amino acids and this region was named dom ...
Computer programs for the analysis and the management of DNA
... are part of the general data management system to be described in the next section. The possibility of double entry ensures that the DNA sequences are correctly typed. Program CHECK can then be used to look for typing errors. Reading data from magnetic tapes, punched tapes or punch cards is also pos ...
... are part of the general data management system to be described in the next section. The possibility of double entry ensures that the DNA sequences are correctly typed. Program CHECK can then be used to look for typing errors. Reading data from magnetic tapes, punched tapes or punch cards is also pos ...