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Microbiology bio 123
... T-A has a double H bond G-C has a triple H bond RNA 1. Two main differences between RNA and DNA 1. Uracil substitutes for Thymine, 2. Single stranded, 2. There are more than one kind of RNA, their structure is the same but their function is not. 3. Sugar base is different, still a five carbon sugar, ...
... T-A has a double H bond G-C has a triple H bond RNA 1. Two main differences between RNA and DNA 1. Uracil substitutes for Thymine, 2. Single stranded, 2. There are more than one kind of RNA, their structure is the same but their function is not. 3. Sugar base is different, still a five carbon sugar, ...
limited warranty
... antibiotics on the day before transfection. Incubate the cells at 37 °C and 5% CO2. The plate should be 60~80% confluent on the day of transfection. One hour before transfection, the serum-containing medium is replaced with 360 µl Opti-Medium (Invitrogen) or DMEM serum-free medium. Step 2: For each ...
... antibiotics on the day before transfection. Incubate the cells at 37 °C and 5% CO2. The plate should be 60~80% confluent on the day of transfection. One hour before transfection, the serum-containing medium is replaced with 360 µl Opti-Medium (Invitrogen) or DMEM serum-free medium. Step 2: For each ...
Ch. 13 Bioengineering
... The Tools of Molecular Biology • Separating DNA – In gel electrophoresis, DNA fragments are placed at one end of a porous gel, and an electric voltage is applied to the gel. – When the power is turned on, the negatively-charged DNA molecules move toward the positive end of the gel. ...
... The Tools of Molecular Biology • Separating DNA – In gel electrophoresis, DNA fragments are placed at one end of a porous gel, and an electric voltage is applied to the gel. – When the power is turned on, the negatively-charged DNA molecules move toward the positive end of the gel. ...
3 - socesbio.c…
... You must find all FIVE and put a single line through them (see left), because they are not used in making the proteins. 5. mRNA codon Amino Acid: With this done, you should have sets of 3 base pairs called CODONS ready to be made into Amino Acids as they match with tRNA. Use the sheet titled CODON ...
... You must find all FIVE and put a single line through them (see left), because they are not used in making the proteins. 5. mRNA codon Amino Acid: With this done, you should have sets of 3 base pairs called CODONS ready to be made into Amino Acids as they match with tRNA. Use the sheet titled CODON ...
Proteins Denaturation
... In gel electrophoresis we use sodium dodecyl sulfate (SDS) to improve the separation according to the differences of masses. The sample separate according to size and net charge. Visulazation is done by staining the membrane (cellulose acetate) by ponsceaus S stain which gives red color or staining ...
... In gel electrophoresis we use sodium dodecyl sulfate (SDS) to improve the separation according to the differences of masses. The sample separate according to size and net charge. Visulazation is done by staining the membrane (cellulose acetate) by ponsceaus S stain which gives red color or staining ...
1 - socesbio.c…
... You must find all FIVE and put a single line through them (see left), because they are not used in making the proteins. 5. mRNA codon Amino Acid: With this done, you should have sets of 3 base pairs called CODONS ready to be made into Amino Acids as they match with tRNA. Use the sheet titled CODON ...
... You must find all FIVE and put a single line through them (see left), because they are not used in making the proteins. 5. mRNA codon Amino Acid: With this done, you should have sets of 3 base pairs called CODONS ready to be made into Amino Acids as they match with tRNA. Use the sheet titled CODON ...
BFP401
... A robot is a programmed computer which can do many jobs at a time and also can be reprogrammed for different uses. A robot can drill holes of several different sizes, sort vegetables, shear sheep, pluck chickens, form rice cakes and assemble mechanical parts. ...
... A robot is a programmed computer which can do many jobs at a time and also can be reprogrammed for different uses. A robot can drill holes of several different sizes, sort vegetables, shear sheep, pluck chickens, form rice cakes and assemble mechanical parts. ...
DNA - The Double Helix
... make up your skin, your hair, and parts of individual cells. The proteins that are made largely determine how you look. The proteins that will be made for your body are determined by the sequence of DNA in the nucleus. Chromosomes are composed of genes, which is a segment of DNA that codes for a par ...
... make up your skin, your hair, and parts of individual cells. The proteins that are made largely determine how you look. The proteins that will be made for your body are determined by the sequence of DNA in the nucleus. Chromosomes are composed of genes, which is a segment of DNA that codes for a par ...
Nucleic acids
... as the building blocks for an organism, proteins make up your skin, your hair, and parts of individual cells. The proteins that are made largely determine how you look. The proteins that will be made for your body are determined by the sequence of DNA in the nucleus. Chromosomes are composed of gene ...
... as the building blocks for an organism, proteins make up your skin, your hair, and parts of individual cells. The proteins that are made largely determine how you look. The proteins that will be made for your body are determined by the sequence of DNA in the nucleus. Chromosomes are composed of gene ...
Demo notes update - UK Association for Science and Discovery
... Get everyone to put the lids on their tanks, plug in the leads and turn on their power packs at the same time. The power packs should be set for 130V, 30 minutes. ...
... Get everyone to put the lids on their tanks, plug in the leads and turn on their power packs at the same time. The power packs should be set for 130V, 30 minutes. ...
DNA - JSH BIOLOGY with Ms. Barbanel
... a. DNA can be visualized through the process of electrophoresis b. In the lab, DNA molecules are cut by restriction enzymes into fragments of various sizes. Restriction enzymes cut at specific sequences throughout the DNA. c. The resulting fragments are forced to move along a gel-coated plate under ...
... a. DNA can be visualized through the process of electrophoresis b. In the lab, DNA molecules are cut by restriction enzymes into fragments of various sizes. Restriction enzymes cut at specific sequences throughout the DNA. c. The resulting fragments are forced to move along a gel-coated plate under ...
Supplementary Notes - Word file (74 KB )
... described6 and is indicated in the schematic of Fig. 1a. In brief, an 18-mer (oligo 1: 5’-ATTCCGATAGTGACTACA-3’) was 5’-32P-labelled using T4 polynucleotide kinase (New England Biolabs) and 50 µCi [-32P]-ATP (GE Healthcare) for 15 mins at 37°C followed by 15 mins chase using 0.5 mM unlabelled ATP. ...
... described6 and is indicated in the schematic of Fig. 1a. In brief, an 18-mer (oligo 1: 5’-ATTCCGATAGTGACTACA-3’) was 5’-32P-labelled using T4 polynucleotide kinase (New England Biolabs) and 50 µCi [-32P]-ATP (GE Healthcare) for 15 mins at 37°C followed by 15 mins chase using 0.5 mM unlabelled ATP. ...
DNA
... Restriction Enzymes – proteins that cut DNA at specific sequences; used for GMOs and DNA fingerprinting; DNA “scissors” – Can cut straight across (blunt end) or in a zig zag fashion (sticky end); sticky ends are useful if you want to put the DNA back together ...
... Restriction Enzymes – proteins that cut DNA at specific sequences; used for GMOs and DNA fingerprinting; DNA “scissors” – Can cut straight across (blunt end) or in a zig zag fashion (sticky end); sticky ends are useful if you want to put the DNA back together ...
make a mammal project
... You must find all FIVE and put a single line through them (see left), because they are not used in making the proteins. 5. mRNA codon Amino Acid: With this done, you should have sets of 3 base pairs called CODONS ready to be made into Amino Acids as they match with tRNA. Use the sheet titled CODON ...
... You must find all FIVE and put a single line through them (see left), because they are not used in making the proteins. 5. mRNA codon Amino Acid: With this done, you should have sets of 3 base pairs called CODONS ready to be made into Amino Acids as they match with tRNA. Use the sheet titled CODON ...
Holiday time test notes
... paternal chromosomes for each of the 23 pairs... 46 total. A primary spermatocyte or oocyte prepares for meiosis just as it would for mitosis... by replicating all of its DNA. After DNA replication there are 92 DNA molecules residing on 46 chromosomes, each consisting of 2 identical sister chromatid ...
... paternal chromosomes for each of the 23 pairs... 46 total. A primary spermatocyte or oocyte prepares for meiosis just as it would for mitosis... by replicating all of its DNA. After DNA replication there are 92 DNA molecules residing on 46 chromosomes, each consisting of 2 identical sister chromatid ...
DNA Extraction, PCR Amplification and Sequencing: the IGS
... following recipe: 2.5 µl of 10X PCR buffer (containing 15 mM MgCl2), 2 µl of 2.5 mM each of all four dNTPs, 1 µl of each primer (10 µM), and 0.1 µl of Platinum Taq (Invitrogen) (5 U/µl); reactions were brought to a final volume of 25 µl with water. Because of the length of the IGS region, and the po ...
... following recipe: 2.5 µl of 10X PCR buffer (containing 15 mM MgCl2), 2 µl of 2.5 mM each of all four dNTPs, 1 µl of each primer (10 µM), and 0.1 µl of Platinum Taq (Invitrogen) (5 U/µl); reactions were brought to a final volume of 25 µl with water. Because of the length of the IGS region, and the po ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.