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Thesis
Thesis

... in sequence, expression and chromatin structure. One approach that has been proposed to investigate the genetic determinants of the adaptation is to analyze the nucleotide differentiation between populations submitted to contrasted environment by whole genome sequencing of pooled individuals. Pools ...
Tools for Comparing Bacterial Genomes
Tools for Comparing Bacterial Genomes

... the chromosome. These happen to be the leading strand during replication. Replication starts at the origin, (the 12 o’clock position here), and proceeds on either side along the circle with both a leading and lagging strand until the bubble reaches the terminus, at 6 o’clock, and the ends are combin ...
Analysis of genetic systems using experimental evolution and whole
Analysis of genetic systems using experimental evolution and whole

... Evolution acts on biological function, and in viruses functions are often mapped to single genes. In complex cellular life forms such as bacteria and yeast, however, complex functions are typically attributed to modules of genes [27]. We might expect, therefore, that parallel evolution for cellular ...
Document
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... to the hypothesis that their adaptation to different aphid species is an ongoing process. ...
HW3 - solutions
HW3 - solutions

... b. A researcher is about to sequence 50 tomato mRNA samples in order to understand the developmental process of the young plant. The researcher wants to have a good estimate on how many reads she needs to sequence in each sample in order to get a good measure of the gene expression in each sample. T ...
BIOVAL - Instituto de Investigación Sanitaria La Fe
BIOVAL - Instituto de Investigación Sanitaria La Fe

... to each type of project This partnership furthers previous fruitful collaboration in the area of bacterial genomics which is also expected to grow next year. NGS technologies allow generating large amounts of fast and cost-effective sequence data facilitating the approach to new applications such as ...
Document
Document

... • Traditionally used parental information • Progeny = ½ mother + ½ father DNA • Progeny = average of mother & father BVs • Assumed full sibs were identical • Available SNP information can be used to supplement the traditional approach • See difference in full-sibs at birth ...
Sathgudi Sweet orange
Sathgudi Sweet orange

... Selected recombinant clones of CMBV (CMBVSON-2, CMBVSON-3, CMBVSON-4, CMBVSON-5 with insert of approximate size ~1075, ~1492 bp, ~1062bp, ~982bp (Table 3.2) and PCR Product (CMBVSON-1, CMBVSON-6, CMBVSON-7, CMBVSON-8 (Table 3.3) with ~1015 bp, ~1306 bp, ~1005 bp, ~833 bp sizes were sequenced at Depa ...
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Chapter 13 Genetics and Biotechnology
Chapter 13 Genetics and Biotechnology

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Genome evolution: a sequence

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ParameciumDB - Nucleic Acids Research
ParameciumDB - Nucleic Acids Research

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Though this isn`t new or different, it is often overlooked

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Pi kur, 2004
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Synthetic Genomics - J. Craig Venter Institute
Synthetic Genomics - J. Craig Venter Institute

...  Virus recovery has been demonstrated using several different synthetic HA and NA gene segments. • Recovery is efficient in 293T/MDCK co-cultures • Next steps include transitioning to rescue in vaccine-approved MDCK cells, in which virus rescue is less efficient. ...
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Supplementary Methods S2: Exome Sequencing

... Detection of Single Nucleotide Variations Single nucleotide mutation detection for both whole genome and capture data was performed using a highly sensitive and specific method called muTector (Cibulskis K. et al, in preparation). In brief, muTector consists of three steps: (i) Preprocessing the ali ...
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No additional copies of HERV-Fc1 in the germ line of multiple

... quantitative and not suited for actual copy-number estimations based on band intensity. Instead, it is an ideal tool for analyzing the potential integration of viral sequence to unknown chromosomal areas, since new integrations will be apparent from the diverse migration distances. Moreover, althoug ...
Syndromic Disorders - Test Code 5007
Syndromic Disorders - Test Code 5007

... infantile neuroaxonal dystrophy, Schinzel-Giedion midface retraction syndrome, DiGeorge syndrome, and choreoacanthocytosis. This NGS test may provide several important benefits for my patient: 1) It may identify the underlying genetics and potentially reveal the true phenotypic spectrum of the of th ...
Draft Genetic and Metabolic Medicine knowledge guide
Draft Genetic and Metabolic Medicine knowledge guide

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GenomicsResourcesForEmergingModelOrganismsPoster
GenomicsResourcesForEmergingModelOrganismsPoster

... emerging model organism communities have accumulated an unprecedented volume of data on sequences, genotypes, expression patterns, etc. Much of this data is from organisms well suited to comparative genomic, evolutionary and ecological studies. More data offers more potential for discovery, but it a ...
Advancements in Genetic Engineering
Advancements in Genetic Engineering

... pretty to form a family, the genetic components in their descendants are limited to sets of combinations. Every aspect of physiologic functions have at most three combinations, once the both parties of couple is determined, whether their kids are healthy or not at a certain aspect is almost unchange ...
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Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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