VWR Taq DNA Polymerase Master Mix
... (final concentration) (2500 reactions) VWR Taq DNA Pol., 1.1 x Master Mix, 2 mM MgCl2 (final concentration) (2500 reactions) VWR Taq DNA Pol., 2 x Master Mix, 1.5 mM MgCl2 (final concentration) (2500 reactions) VWR Taq DNA Pol., 2 x Master Mix, 2 mM MgCl2 (final concentration) (2500 reactions) VWR R ...
... (final concentration) (2500 reactions) VWR Taq DNA Pol., 1.1 x Master Mix, 2 mM MgCl2 (final concentration) (2500 reactions) VWR Taq DNA Pol., 2 x Master Mix, 1.5 mM MgCl2 (final concentration) (2500 reactions) VWR Taq DNA Pol., 2 x Master Mix, 2 mM MgCl2 (final concentration) (2500 reactions) VWR R ...
Preparation and analysis of environmental DNA: optimisation of
... recovering symbiotic, stationary or slow growing organisms, and the so called viable but non-cultivable fraction which are believed to make up the bulk of environmental organisms [1]. The development of DNA-based techniques which rely on analysis of DNA extracted from microorganisms inhabiting a par ...
... recovering symbiotic, stationary or slow growing organisms, and the so called viable but non-cultivable fraction which are believed to make up the bulk of environmental organisms [1]. The development of DNA-based techniques which rely on analysis of DNA extracted from microorganisms inhabiting a par ...
Southern Blotting
... Digests must be complete for results to be interpretable. It is advisable to run test reactions for the first blot, to optimize conditions and times. Incomplete digests are the primary cause of Southern Blot failure. It is particularly important to ensure that DNA has fully dissolved prior to digest ...
... Digests must be complete for results to be interpretable. It is advisable to run test reactions for the first blot, to optimize conditions and times. Incomplete digests are the primary cause of Southern Blot failure. It is particularly important to ensure that DNA has fully dissolved prior to digest ...
Introduction Kit components
... mix thoroughly by vortexing or inverting tube several times. For DNA fragments >4kb or <400bp, add 1 volume of absolute ethanol to the sample. Mix thoroughly. ...
... mix thoroughly by vortexing or inverting tube several times. For DNA fragments >4kb or <400bp, add 1 volume of absolute ethanol to the sample. Mix thoroughly. ...
Crystal structure of Cas9 in complex with guide RNA and target DNA
... technology, which works effectively in various types of cells and organisms. Catalytically dead or inactive Cas9 (referred to as dCas9) can serve as an RNAguided genome-targeting platform, and dCas9-based new technologies, such as those for transcription regulation and chromatin imaging, have also b ...
... technology, which works effectively in various types of cells and organisms. Catalytically dead or inactive Cas9 (referred to as dCas9) can serve as an RNAguided genome-targeting platform, and dCas9-based new technologies, such as those for transcription regulation and chromatin imaging, have also b ...
GeneJET PCR Purification Kit, #K0701, #K0702
... Ensure that the recommended volume of ethanol has been added to the Wash Buffer (concentrated) prior first use (see p. 3). Inefficient DNA elution Add Elution Buffer directly to the center of the membrane and not to the side of the GeneJET purification column. Use 20-50 µL of Elution Buffer and ensu ...
... Ensure that the recommended volume of ethanol has been added to the Wash Buffer (concentrated) prior first use (see p. 3). Inefficient DNA elution Add Elution Buffer directly to the center of the membrane and not to the side of the GeneJET purification column. Use 20-50 µL of Elution Buffer and ensu ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""