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Why Do Names Keep Changing
Why Do Names Keep Changing

... Proteins are strings of amino acids. There are 24 amino acids coded by DNA plus a start and stop signal (total 26) and 4 different types of base. 1 base / codon codes for 4 amino acids 2 bases / codon codes for 16 amino acids 3 bases / codon codes for 64 amino acids Enough! This is the called the tr ...
Chapter 13 Vocabulary Name
Chapter 13 Vocabulary Name

... 3. plasmid: small, circular DNA molecule found in bacteria that is separate from the bacterial chromosome (Concept 13.2) 4. restriction enzyme: enzyme that cuts sugar-phosphate bonds in the DNA backbone at specific points within particular nucleotide sequences in DNA (Concept 13.2) 5. genomic librar ...
Name: Period: ______
Name: Period: ______

... have you thought about what exactly is encoded for by this DNA? How do our cells use DNA as a set of instructions for life? How is the information in our DNA and genes used by our bodies? And what happens when DNA is mutated or not used properly? In this lab, you will model the work of real scientis ...
ampicillin resistance
ampicillin resistance

... It has become an important research organism for molecular biology Many strains commercially available Reproduces very rapidly A single microscopic cell can divide to form a visible colony with millions of cells overnight ...
anth-260-midterm-review-sheet-2016
anth-260-midterm-review-sheet-2016

... • All of the following are true of the relationship between DNA and proteins EXCEPT: a. a sequence of three DNA base-pairs codes for one amino acid b. a single codon codes for one amino acid c. an amino acid is coded by only one codon d. sequences of codons code for sequences of amino acids • A prim ...
Nucleic Acid Biotechnology Techniques
Nucleic Acid Biotechnology Techniques

... • Plasmid vectors pBR322 and pUC are cloning ...
Chapter 9 DNA: THE Genetic Material
Chapter 9 DNA: THE Genetic Material

... double helix – two strands twisted around each other Nucleotides – the subunits that make up DNA 3 parts: a phosphate group, a 5-carbon sugar, and a nitrogencontaining base ...
Restriction Enzyme Worksheet
Restriction Enzyme Worksheet

... restriction enzymes. Restriction enzymes are proteins that bacteria use to cut up DNA that doesn’t belong to them. If a bacterium senses that a virus is trying to invade, or a different species of bacterium represents a threat, it can use a restriction enzyme to cut up the foreigner’s DNA. Restricti ...
how to read a pedigree - Doral Academy Preparatory
how to read a pedigree - Doral Academy Preparatory

... bacteria in the soil & plants to ...
Packet #3
Packet #3

... 6. You have a plasmid with genes for tetracycline resistance and ampicillin resistance, as shown in the diagram 6a. In the middle of the tetracycline resistance gene is a target site for the restriction enzyme BamHI. Therefore, when you insert the gene of interest into this plasmid using the BAMHI ...
DNA: The Molecule Of Life
DNA: The Molecule Of Life

... The Structure of DNA -Is composed of two strands in shape of a double helix. -The strands are arranged in an anti-parallel fashion. -The backbone of DNA is composed of a repeating phosphate deoxyribose polymer - Bases are bonded to the 1' carbon of each molecule of deoxyribose -The strands are held ...
Nucleotide drug targets
Nucleotide drug targets

... Mendelian pattern. Up until now you have had this view that genes are sections of double stranded, double helical DNA that code for one polypeptide chain. This is a very precise and accurate definition but gives no idea of how it exists in the cell. You have been taught that the hereditary material ...
Nucleotide drug targets.
Nucleotide drug targets.

... Mendelian pattern. Up until now you have had this view that genes are sections of double stranded, double helical DNA that code for one polypeptide chain. This is a very precise and accurate definition but gives no idea of how it exists in the cell. You have been taught that the hereditary material ...
Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog
Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog

... insertion of human DNA into bacterial plasmid DNA using DNA ligase to form a recombinant plasmid ...
Quiz 1 - Suraj @ LUMS
Quiz 1 - Suraj @ LUMS

... 1. Which of these scientific terms has the greatest degree of certainty? a) hypothesis; b) theory; c) law; d) guess 2. Which of the following is the least inclusive (smallest) unit of classification? a) kingdom; b) species; c) genus; d) class; e) phylum 3. Bacteria belong to the taxonomic kingdom a) ...
Recombinant DNA
Recombinant DNA

... bacteria with a nonrecombinant plasmid; that is, the lacZ+ gene is intact. White colony contains bacteria with a recombinant plasmid; that is, the vector with an inserted DNA fragment. Once the white colony with the gene of interest is identified, it can be grown in culture to produce large quantiti ...
SBI 4U Genetics 6
SBI 4U Genetics 6

... certain genes with DNA from other areas.  Called recombinant DNA  Bacteria have restriction enzymes that will cut up invading viral DNA.  Scientists can use a special type of restriction enzyme called restriction endonuclease because they cleave double-stranded DNA in the middle of the strand by ...
Glossary Algae: Unicellular or simple multicellular photosynthetic
Glossary Algae: Unicellular or simple multicellular photosynthetic

... structure by which hereditary information is physically transmitted from one generation to the next; in a bacterium, the chromosome consists of a single nacked circle of DNA; in eukaryotes, each chromosome consists of a single linear DNA molecule and associated proteins. Codon bias: Refers to the fa ...
November Syllabus
November Syllabus

... to formulate these laws. ...
Document
Document

... …sticky ends with complementary base pairs can form hydrogen bonds, …DNA ligase: an enzyme that catalyzes the reformation of the phosphodiester bonds. ...
DNA Technology - University of Evansville Faculty Web sites
DNA Technology - University of Evansville Faculty Web sites

... The Process of DNA Fingerprinting a. DNA is isolated from cells and cleaved at specific sites with an endonuclease b. The sample containing DNA fragments from each individual is placed in an electrophoretic gel where the fragments are separated by size and charge, producing a streak of fragments of ...
CH 12: Mendel and Heredity
CH 12: Mendel and Heredity

... 13. What is a point mutation and does it always change the protein that is made? ...
Exploring Genes
Exploring Genes

... used as vectors?  What advantages do these modified viruses have over plasmids? ...
BERRY FULL OF DNA
BERRY FULL OF DNA

... therefore not visible. When molecules are insoluble, they clump together and become visible. The colder the ethanol, the less soluble the DNA will be in it. This is why it is important for the ethanol to be kept in the freezer or in an ice bath. ...
An Introduction to DNA and Genetics Directions: As you watch the
An Introduction to DNA and Genetics Directions: As you watch the

... try to fill in these notes on your own. Or, explore the “more about _____” sections of this website. ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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