Sugopa Sengupta - Presidency University
... Brief Synopsis: My doctoral thesis work involved identification and characterization of three endogenous inhibitors of an essential bacterial enzyme, DNA gyrase. My studies revealed that all these endogenous inhibitors essentially influence the enzyme activity by sequestering the enzyme away from DN ...
... Brief Synopsis: My doctoral thesis work involved identification and characterization of three endogenous inhibitors of an essential bacterial enzyme, DNA gyrase. My studies revealed that all these endogenous inhibitors essentially influence the enzyme activity by sequestering the enzyme away from DN ...
Biotechnology notes
... pick up naked foreign DNA wherever it may be hanging out have surface transport proteins that are ...
... pick up naked foreign DNA wherever it may be hanging out have surface transport proteins that are ...
cloning vectors
... marker (such as the ampicillin resistance gene), (ii) a plasmid origin of replication (ori), (iii) a fragment carrying the ligated cohesive ends (cos) of phage , and (iv) one or more unique restriction sites for cloning (Collins & Hohn, 1978) (Figure 5). Recombinant cosmid molecules can be convenie ...
... marker (such as the ampicillin resistance gene), (ii) a plasmid origin of replication (ori), (iii) a fragment carrying the ligated cohesive ends (cos) of phage , and (iv) one or more unique restriction sites for cloning (Collins & Hohn, 1978) (Figure 5). Recombinant cosmid molecules can be convenie ...
Chapter 5A
... for DNA cloning in a plasmid vector is presented in Fig. 5.14. In Step 1, the DNA of interest is ligated into a plasmid cloning vector. In Step 2, the recombinant plasmid is introduced into E. coli host cells by transformation. In Step 3, cells that have taken up the plasmid are selected on antibiot ...
... for DNA cloning in a plasmid vector is presented in Fig. 5.14. In Step 1, the DNA of interest is ligated into a plasmid cloning vector. In Step 2, the recombinant plasmid is introduced into E. coli host cells by transformation. In Step 3, cells that have taken up the plasmid are selected on antibiot ...
Molecular Biology
... marker (such as the ampicillin resistance gene), (ii) a plasmid origin of replication (ori), (iii) a fragment carrying the ligated cohesive ends (cos) of phage λ, and (iv) one or more unique restriction sites for cloning (Collins & Hohn, 1978) (Figure 5). Recombinant cosmid molecules can be convenie ...
... marker (such as the ampicillin resistance gene), (ii) a plasmid origin of replication (ori), (iii) a fragment carrying the ligated cohesive ends (cos) of phage λ, and (iv) one or more unique restriction sites for cloning (Collins & Hohn, 1978) (Figure 5). Recombinant cosmid molecules can be convenie ...
Unit VII Study Guide KEY
... may be synthesized in a _functional__________ form, meaning transcription will be blocked, or in a _dysfunctional__________ form, meaning transcription can proceed. The _lac____ operon is a(n) _inducible______ operon, meaning it is normally off. Its repressor is synthesized in a _functional______ fo ...
... may be synthesized in a _functional__________ form, meaning transcription will be blocked, or in a _dysfunctional__________ form, meaning transcription can proceed. The _lac____ operon is a(n) _inducible______ operon, meaning it is normally off. Its repressor is synthesized in a _functional______ fo ...
Genetics
... Putting DNA to Work Explores the role of DNA in disease detection, crop modifications, and forensics. Tutorials are supplemented by activities allowing for exploration of DNA sequences, genetic testing, blood analyses, microarrays, and mutations. (Marian Koshland Science Museum, The National Academi ...
... Putting DNA to Work Explores the role of DNA in disease detection, crop modifications, and forensics. Tutorials are supplemented by activities allowing for exploration of DNA sequences, genetic testing, blood analyses, microarrays, and mutations. (Marian Koshland Science Museum, The National Academi ...
Genetic Engineering
... lungs fill with a thick, sticky mucus which, apart from producing breathing difficulties, makes the person concerned very prone to bacterial infections. By using viruses or small lipid particles as vectors, it is hoped to be able to insert a functional copy of the gene into the lung cells so that th ...
... lungs fill with a thick, sticky mucus which, apart from producing breathing difficulties, makes the person concerned very prone to bacterial infections. By using viruses or small lipid particles as vectors, it is hoped to be able to insert a functional copy of the gene into the lung cells so that th ...
PUTTING DNA to WORK: High School Virtual Field Trip
... 6. Which was faster and easier to use in finding a match for the human parainfluenza virus 3, the method using DNA letter sequences or the method using bar codes? ...
... 6. Which was faster and easier to use in finding a match for the human parainfluenza virus 3, the method using DNA letter sequences or the method using bar codes? ...
Protein Synthesis - Manhasset Public Schools
... 1. Transcription: the copying of the genetic message (DNA) into a molecule of mRNA 2. Translation: mRNA is used to assemble an amino acid sequence into a polypeptide ...
... 1. Transcription: the copying of the genetic message (DNA) into a molecule of mRNA 2. Translation: mRNA is used to assemble an amino acid sequence into a polypeptide ...
EcoRI
... breakage of that chromosome; genetic information will be lost Duplication (2)- results in the copying of a segment of the chromosome Inversion (3)- a segment of a chromosome breaks off and reattaches itself to the chromosome in a reversed order ...
... breakage of that chromosome; genetic information will be lost Duplication (2)- results in the copying of a segment of the chromosome Inversion (3)- a segment of a chromosome breaks off and reattaches itself to the chromosome in a reversed order ...
DNA Cornell notes
... strands of DNA are built from the template strand, using DNA polymerase (enzyme) to bring in the nucleotides. Nucleotides on the leading strands are brought to the template strand in a continuous fashion. Nucleotides on the lagging strand are brought in segments known as Okazaki fragments. The Okaza ...
... strands of DNA are built from the template strand, using DNA polymerase (enzyme) to bring in the nucleotides. Nucleotides on the leading strands are brought to the template strand in a continuous fashion. Nucleotides on the lagging strand are brought in segments known as Okazaki fragments. The Okaza ...
Chromosomes and DNA Packaging
... NOTE: if histones from different species are added to any eukaryotic DNA sample, chromatin is reconstituted. Implication? Very highly conserved in eukaryotes in both ...
... NOTE: if histones from different species are added to any eukaryotic DNA sample, chromatin is reconstituted. Implication? Very highly conserved in eukaryotes in both ...
MICR 201 Microbiology for Health Related Sciences
... ▪ Some bacteria can degrade viruses with these enzyme and are protected against these viruses ...
... ▪ Some bacteria can degrade viruses with these enzyme and are protected against these viruses ...
Aim 24: How does DNA code for the production of proteins through
... Once transcription in the nucleus occurs, the mRNA that is created travels to a ribosome. Step 1: ___________ leaves the ____________________ and travels to a __________________ Step 2: The ribosome travels along the mRNA strand as a specific _______________________ is carried to the mRNA ...
... Once transcription in the nucleus occurs, the mRNA that is created travels to a ribosome. Step 1: ___________ leaves the ____________________ and travels to a __________________ Step 2: The ribosome travels along the mRNA strand as a specific _______________________ is carried to the mRNA ...
Applications of Molecular Biology in Archaeology
... as 2,400 yr-old for DNA and found that DNA could be molecularly cloned in a plasmid vector. Problem: Classical molecular biological methodologies required high amounts of DNA material, but most ancient DNA are highly degraded and only minute quantities may be extracted ...
... as 2,400 yr-old for DNA and found that DNA could be molecularly cloned in a plasmid vector. Problem: Classical molecular biological methodologies required high amounts of DNA material, but most ancient DNA are highly degraded and only minute quantities may be extracted ...
Recombinant Plasmids
... (hence the R) to antibiotics that would normally kill them. From the use of antibiotics, bacteria can be killed when it lacks the R. plasmid, while those with an R. plasmid tend to multiply. As a result, an increasing number of bacteria that cause human diseases, like food poisoning and gonorrhea ar ...
... (hence the R) to antibiotics that would normally kill them. From the use of antibiotics, bacteria can be killed when it lacks the R. plasmid, while those with an R. plasmid tend to multiply. As a result, an increasing number of bacteria that cause human diseases, like food poisoning and gonorrhea ar ...
C.P. Biology Study Guide for the Final Exam
... 3. In photosynthesis, energy is stored in the form of glucose within the cell. What was the original source of energy used to do this?__________________________ 4. Summarize the light-dependent reaction and identify where it occurs. ...
... 3. In photosynthesis, energy is stored in the form of glucose within the cell. What was the original source of energy used to do this?__________________________ 4. Summarize the light-dependent reaction and identify where it occurs. ...
Polymerase Chain Reaction (PCR)
... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
Biology II – Chapter 9: DNA: The Molecule of Heredity
... o Definition of translation: to convert words of one language into another o The process of protein synthesis from RNA is called translation. Ribosomes carry out the process of translation o The number of different proteins that make up an organism depends on the organism’s complexity. o Proteins ...
... o Definition of translation: to convert words of one language into another o The process of protein synthesis from RNA is called translation. Ribosomes carry out the process of translation o The number of different proteins that make up an organism depends on the organism’s complexity. o Proteins ...
Using DNA to Classify Life
... Count the number of loops (areas of difference) Count the number of clips that do not touch (number of differences) ...
... Count the number of loops (areas of difference) Count the number of clips that do not touch (number of differences) ...
Genetics - Wantagh School
... 2 Important Laws of Genetics • Organisms inherit genes in pairs, one gene from each parent 1. Meiosis= gene pairs separate during the sex cell formation (egg or sperm cell) 2. Independent Assortment = each gene pair for a trait is inherited independently of the gene pairs for all other traits ...
... 2 Important Laws of Genetics • Organisms inherit genes in pairs, one gene from each parent 1. Meiosis= gene pairs separate during the sex cell formation (egg or sperm cell) 2. Independent Assortment = each gene pair for a trait is inherited independently of the gene pairs for all other traits ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.