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DNA - Paxon Biology
DNA - Paxon Biology

... DNA is a double helix, however, only 1 of these strands is transcribed. The strand that is transcribed is called the template strand. The other strand is NOT transcribed and is called the non-template strand; it serves as a template for making a new strand during replication. An mRNA will be complem ...
DNA TEST, PART 2: DNA MESSAGE DECODING You will be given
DNA TEST, PART 2: DNA MESSAGE DECODING You will be given

... FIRST: Put your name, seat number, date, and period at top of page. SECOND: copy the number of your message and the DNA message itself in the spaces so designated. THIRD: decode the message, showing each step completely, just as it happens in your cells; be sure to label each step with the type of m ...
Plasmids and DNA Digestion
Plasmids and DNA Digestion

... form gels that are suitable for DNA electrophoresis. Electrophoresis: A method of separation, typically for DNA or proteins, which uses the charge of the molecules to drive separation. Smaller molecules move faster through the gel matrix than large ones. Ethidium Bromide: A compound frequently used ...
DNA Replication - susanpittinaro
DNA Replication - susanpittinaro

... T.H. Morgan • Conclusion • Genes are on chromosomes • But is it the protein or the DNA of the chromosome that codes for the gene? • Initially proteins were thought to contain genetic information … why? What’s so impressive about proteins?! ...
DNA Replication
DNA Replication

... DNA and Chromosomes • Many eukaryotes have 1000 times the amount of DNA as prokaryotes. • Eukaryotic DNA is located in the cell nucleus inside chromosomes. • The number of chromosomes varies widely from one species to the next. ...
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... – Duplex formation Tm = 63 °C (22 ° higher than same DNA or RNA sequence!) –  GNA more stable than DNA! – Demonstrates that cyclic sugar not necessary! ...
DNA (Gene) Mutations
DNA (Gene) Mutations

... more) missing, added, or incorrect A mistake in the genetic code Wrong instructions  wrong building materials  wrong structure. ...
Unit 1 Ch. 1, 17, 18. WHAT IS BIOLOGY?
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DNA Mutations ppt
DNA Mutations ppt

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Genomes
Genomes

... transcription. RNA polymerase doesn’t need primers. Three distinct RNA polymerases carry out DNA-dependent synthesis of RNA  RNA polymerase I  RNA polymerase II  RNA polymerase III Template- DNA The coding (nontemplate) strand of the DNA is identical in base sequence to the RNA transcribed from t ...
DNA (Gene) Mutations
DNA (Gene) Mutations

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May 27, 2017 The Difference Makers
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... from making DNA versions from their RNA copies. Other normal cellular functions do not typically use retrotransposase or reverse transcriptase, so such an inhibitor should not interfere with other functions. Inhibitors that target enzymes involved in splicing transposon copies into the genome might ...
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... protein. They are NOT transcribed *  Exons – sequences in the DNA that are expressed or used to make mRNA and and ultimately are used to make a protein ...
Gene Polypeptide - Grayslake Central High School
Gene Polypeptide - Grayslake Central High School

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Cross-Curricular Discussion
Cross-Curricular Discussion

... from making DNA versions from their RNA copies. Other normal cellular functions do not typically use retrotransposase or reverse transcriptase, so such an inhibitor should not interfere with other functions. Inhibitors that target enzymes involved in splicing transposon copies into the genome might ...
1 - Biology Mad
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... The inheritance of the ability to produce hydrogen cyanide is controlled by two genes which are located on different chromosomes. The dominant allele of one gene, G, controls the production of enzyme G which converts a precursor to linamarin. The dominant allele of the other gene, E, controls the pr ...
F: Acronyms and Glossary
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... Introns: DNA sequences interrupting the protein-coding DNA sequences of a gene that are transcribed into mRNA, but are spliced out of the rnRNA before the rnRNA is translated into protein. Compare exons. Karyotype: A photomicrograph of an individual’s chromosomes arranged in a standard format showin ...
DNA Transcription and Translation
DNA Transcription and Translation

... In the process of transcription, a gene is copied and the information is taken out of the  nucleus so that the cell can make a protein out of the information obtained from the  DNA in the gene ­ Every 3 nitrogen bases in the DNA that makes up a gene is called a codon, and codes  for a specific amino ...
Chapter 20~ DNA Technology & Genomics
Chapter 20~ DNA Technology & Genomics

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... the amino acid chain grows. – The mRNA is read codon by codon and the protein is built up one amino acid at a time in the correct sequence. ...
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... proteins.A protein is composed of smaller molecules called amino acids, and the structure and function of the protein is determined by the sequence of its amino acids. The sequence of amino acids, in turn, is determined by the sequence of nucleotide bases in the DNA. A sequence of three nucleotide b ...
Bio 5924: Molecular Basis of Heredity
Bio 5924: Molecular Basis of Heredity

... Engineering), Barak Cohen (Genetics), Tim Ley (Internal Medicine), Jeff Gordon (Developmental Biology), and Robi Mitra (Genetics) Teaching Assistants: Taylor Cordonnier, Matthew Dothager, Stephen McDaniel, Andrew Nylander, Priya Srikanth ...
Restriction Enzymes
Restriction Enzymes

... Southern Blotting • A technique used in molecular biology to check for the presence of a particular DNA sequence in a DNA sample. • The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. ...
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MyTaq™ HS DNA Polymerase
MyTaq™ HS DNA Polymerase

... The leaves from plants such as Arabidopsis thaliana, corn and tomato are used for agricultural research and are a ready source of DNA without causing too much damage to the main plant. The use of ISOLATE II and MyTaq means that high quality DNA can be extracted from many leaves and then used in PCR ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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