Lecture3 (1/22/08) "Nucleic Acids, RNA, and Proteins"
... 3. Why is one end of DNA called 5’? The other 3’? -- the nomenclature of sugar #’s. (today) 4. More about DNA folding – why a meter long can compact into a few microns -- have a special section on DNA bending and twisting with magnetic traps next time or timeafter. 5. What if mis-match: how fix it? ...
... 3. Why is one end of DNA called 5’? The other 3’? -- the nomenclature of sugar #’s. (today) 4. More about DNA folding – why a meter long can compact into a few microns -- have a special section on DNA bending and twisting with magnetic traps next time or timeafter. 5. What if mis-match: how fix it? ...
Slides - nanoHUB
... 3. Why is one end of DNA called 5’? The other 3’? -- the nomenclature of sugar #’s. (today) 4. More about DNA folding – why a meter long can compact into a few microns -- have a special section on DNA bending and twisting with magnetic traps next time or timeafter. 5. What if mis-match: how fix it? ...
... 3. Why is one end of DNA called 5’? The other 3’? -- the nomenclature of sugar #’s. (today) 4. More about DNA folding – why a meter long can compact into a few microns -- have a special section on DNA bending and twisting with magnetic traps next time or timeafter. 5. What if mis-match: how fix it? ...
short_answer_Barcoding_exam_Key
... size, and then a laser reads the results to indicate the sequence 38. What is unique about the ddNTPS that make them useful in DNA sequencing? (3) The oxygen molecule is not present, so a covalent bond with another nucleotide at that the phosphate can’t occur, which causes elongation to stop at vari ...
... size, and then a laser reads the results to indicate the sequence 38. What is unique about the ddNTPS that make them useful in DNA sequencing? (3) The oxygen molecule is not present, so a covalent bond with another nucleotide at that the phosphate can’t occur, which causes elongation to stop at vari ...
Agrobacterium tumefaciens
... most common) kind of ends produced (5’ or 3’ overhang (sticky), blunt) degenerate or specific sequences whether cleavage occurs within the recognition sequence ...
... most common) kind of ends produced (5’ or 3’ overhang (sticky), blunt) degenerate or specific sequences whether cleavage occurs within the recognition sequence ...
Multi-copy suppressor screen
... duplicated (during replication). To enable a piece of DNA lacking an ARS to replicate, you need to insert it into the yeast chromosome where it will be replicated using chromosomal ARS elements adjacent to the site of integration. In yeast recombination is almost always homologous, so that the trans ...
... duplicated (during replication). To enable a piece of DNA lacking an ARS to replicate, you need to insert it into the yeast chromosome where it will be replicated using chromosomal ARS elements adjacent to the site of integration. In yeast recombination is almost always homologous, so that the trans ...
Solutions to 7.014 Problem Set 7
... b) For each of the following cohorts (group of individuals born at the same time), decide which type of survivorship curve best applies. Explain briefly. i) Cohort of 20 sugar maple seedlings growing in the shadow of the parent, a tall sugar maple in mature forest. Type III curve best applies, since ...
... b) For each of the following cohorts (group of individuals born at the same time), decide which type of survivorship curve best applies. Explain briefly. i) Cohort of 20 sugar maple seedlings growing in the shadow of the parent, a tall sugar maple in mature forest. Type III curve best applies, since ...
Evolution of DNA by celluLar automata HC Lee Department of
... • Reality is complex, but models don't have to be • Von Neumann machines - a machine capable of reproduction; the basis of life is information – Stanislaw Ulam: build the machine on paper, as a collection of cells on a lattice ...
... • Reality is complex, but models don't have to be • Von Neumann machines - a machine capable of reproduction; the basis of life is information – Stanislaw Ulam: build the machine on paper, as a collection of cells on a lattice ...
The Nucleus: DNA, Chromatin And Chromosomes
... - Ribosomes are the machines that synthesize proteins based on the m-RNA template. Ribosomes are non-specific i.e. they are used in the synthesis of all proteins. iii. Transfer-RNA (t-RNA) - Transfers the appropriate amino-acid to a growing protein chain - There is one t-RNA for each amino-acid ...
... - Ribosomes are the machines that synthesize proteins based on the m-RNA template. Ribosomes are non-specific i.e. they are used in the synthesis of all proteins. iii. Transfer-RNA (t-RNA) - Transfers the appropriate amino-acid to a growing protein chain - There is one t-RNA for each amino-acid ...
Final Exam Review
... Final Exam Review Biology 104 The exam will cover all of our chapters but remember as you review chapters how the concepts build upon one another. For example, the chapter on chemistry and biological molecules was preparing us to study respiration and photosynthesis. Emphasis for the final will be o ...
... Final Exam Review Biology 104 The exam will cover all of our chapters but remember as you review chapters how the concepts build upon one another. For example, the chapter on chemistry and biological molecules was preparing us to study respiration and photosynthesis. Emphasis for the final will be o ...
General
... sequence checking can permit selection of appropriate enzymes for subcloning or generation of probes ...
... sequence checking can permit selection of appropriate enzymes for subcloning or generation of probes ...
Genetics
... DNA copying is a blind process It can be hijacked, e.g. by viruses It makes mistakes Mistakes accumulate over (lots of) time ...
... DNA copying is a blind process It can be hijacked, e.g. by viruses It makes mistakes Mistakes accumulate over (lots of) time ...
Genetic aspects of Multiple Sclerosis Boon, Maartje
... Epistasis: process of two or more genes interacting with each other Exon: part of a gene that is expressed by transcription into mRNA Gene: sequence of DNA nucleotide bases, coding for a polypeptide. Individual unit of heredity Genetic drift: random process leading to increase in frequency of some a ...
... Epistasis: process of two or more genes interacting with each other Exon: part of a gene that is expressed by transcription into mRNA Gene: sequence of DNA nucleotide bases, coding for a polypeptide. Individual unit of heredity Genetic drift: random process leading to increase in frequency of some a ...
Chapter 17. Application of Recombinant DNA Technology in
... • The most useful restriction enzymes cut DNA in a staggered way, producing fragments with “sticky ends” that bond with complementary sticky ends of other fragments. • DNA ligase is an enzyme that seals the bonds between ...
... • The most useful restriction enzymes cut DNA in a staggered way, producing fragments with “sticky ends” that bond with complementary sticky ends of other fragments. • DNA ligase is an enzyme that seals the bonds between ...
14 Diversity of BCR BA
... How is an infinite diversity of specificity generated from finite amounts of DNA? Combinatorial diversity ...
... How is an infinite diversity of specificity generated from finite amounts of DNA? Combinatorial diversity ...
Answers - loreescience.ca
... comparison of VNTR DNA in the samples rather than the DNA found in the genes. Explain why you think this is so. The characteristics of VNTR microsatellites (the DNA of which is non-coding) differ widely between different individuals. On the other hand, because variation in base sequence often has su ...
... comparison of VNTR DNA in the samples rather than the DNA found in the genes. Explain why you think this is so. The characteristics of VNTR microsatellites (the DNA of which is non-coding) differ widely between different individuals. On the other hand, because variation in base sequence often has su ...
Level 2 Biology - No Brain Too Small
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
Document
... Oligonucleotides match each of the common and variant alleles in the population (all alleles of interest). ...
... Oligonucleotides match each of the common and variant alleles in the population (all alleles of interest). ...
Mutation - TeacherWeb
... Each cell of in organism have the same number of chromosomes, except the sex cells ...
... Each cell of in organism have the same number of chromosomes, except the sex cells ...
Protein Synthesis - No Brain Too Small
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
... Compare and contrast these two cell processes and their role in protein synthesis. In your answer: ...
Nerve activates contraction
... haploid set of chromosomes • Multigene families exist as a collection of identical or very similar genes (exceptions). • These likely evolved from a single ancestral gene. • The members of multigene families may be clustered or dispersed in the genome. ...
... haploid set of chromosomes • Multigene families exist as a collection of identical or very similar genes (exceptions). • These likely evolved from a single ancestral gene. • The members of multigene families may be clustered or dispersed in the genome. ...
DNA structure
... • Nucleotides match to template according to base pairing rules (complementary strand) • 1 ‘parent’ DNA strand produces 2 new ‘daughter’ strands • Occurs rapidly, both strands simultaneously – Humans with 6 billion pairs a few hours, with only about 1 error every 10 billion nucleotides ...
... • Nucleotides match to template according to base pairing rules (complementary strand) • 1 ‘parent’ DNA strand produces 2 new ‘daughter’ strands • Occurs rapidly, both strands simultaneously – Humans with 6 billion pairs a few hours, with only about 1 error every 10 billion nucleotides ...
Mutations
... bulges, kinks, or similar damage to DNA • Chunk of DNA containing the damaged area is excised, replaced by DNA polymerase I enzyme (or equivalent) – 13 bases removed in bacteria – Eukaryotes (always more elaborate) take out 28 nucleotides – In humans, failure in this repair system causes disease xer ...
... bulges, kinks, or similar damage to DNA • Chunk of DNA containing the damaged area is excised, replaced by DNA polymerase I enzyme (or equivalent) – 13 bases removed in bacteria – Eukaryotes (always more elaborate) take out 28 nucleotides – In humans, failure in this repair system causes disease xer ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.