GENETICS 603 Exam 1, September 27, 2013 1. Which of the
... 12. A series of different HFR strains of E. coli that could all grow on minimal medium with either maltose or mannose were allowed to mate for 40 minutes with an F-‐ strains with mutations ...
... 12. A series of different HFR strains of E. coli that could all grow on minimal medium with either maltose or mannose were allowed to mate for 40 minutes with an F-‐ strains with mutations ...
Application of Microarray- Based Genomic Technology to Mutation
... • Genotyping sequence variations, • Single-base mismatch prevents ligation, • A G/T mismatch at the 3’- end to be ligated inhibits the reaction by up to 1,000-fold. ...
... • Genotyping sequence variations, • Single-base mismatch prevents ligation, • A G/T mismatch at the 3’- end to be ligated inhibits the reaction by up to 1,000-fold. ...
General Biology I Test V
... • Sequence of amino acids correlates linearly with sequences of mutations. • If nucleotide overlaps, each single nucleotide change would yield more than one amino acid change, however protein sequencing finds only one amino acid change. ...
... • Sequence of amino acids correlates linearly with sequences of mutations. • If nucleotide overlaps, each single nucleotide change would yield more than one amino acid change, however protein sequencing finds only one amino acid change. ...
Chap2 DNA RNA and Protein
... The genetic information is stored in the alignment and sequence of these 4 bases, analogous to 0 and 1 used in the information storage in computer. The sequence of the DNA determines the polypeptide sequence and the protein function and hence the cellular activities and functions. ...
... The genetic information is stored in the alignment and sequence of these 4 bases, analogous to 0 and 1 used in the information storage in computer. The sequence of the DNA determines the polypeptide sequence and the protein function and hence the cellular activities and functions. ...
Protocol: Cloning of oligos for sgRNA (CRISPR) or
... This protocol allows you to clone oligos to generate shRNAs or sgRNAs on a small scale. -For sgRNAs, pXPR vectors with a single BsmBI or BbsI cloning site are most common, two types of pXPR vectors can be used: 1. pXPR_003 (or “lenti guide”) will only contain an sgRNA and is to be used in a cell lin ...
... This protocol allows you to clone oligos to generate shRNAs or sgRNAs on a small scale. -For sgRNAs, pXPR vectors with a single BsmBI or BbsI cloning site are most common, two types of pXPR vectors can be used: 1. pXPR_003 (or “lenti guide”) will only contain an sgRNA and is to be used in a cell lin ...
DNA
... C. Describe the process of DNA replication D. Describe the steps of translation and transcription in changing DNA into traits E. Describe the effect of DNA mutations and list genetic diseases that would result F. Debate the use of genetic technologies in ...
... C. Describe the process of DNA replication D. Describe the steps of translation and transcription in changing DNA into traits E. Describe the effect of DNA mutations and list genetic diseases that would result F. Debate the use of genetic technologies in ...
Work Day 2
... Ciprofloxacin blocks DNA synthesis. Downstream steps of gene expression do not occur. Treatment of ciprofloxacin-sensitive bacteria with the antibiotic results in cell death. ...
... Ciprofloxacin blocks DNA synthesis. Downstream steps of gene expression do not occur. Treatment of ciprofloxacin-sensitive bacteria with the antibiotic results in cell death. ...
Chapter 16 Presentation
... • If it followed the dispersive model, one band would be seen containing hybrid DNA. This was seen after the first replication, but not the second*. • If it followed the semiconservative model one band would be seen after the first replication and 2 would be seen after the 2nd replication. ...
... • If it followed the dispersive model, one band would be seen containing hybrid DNA. This was seen after the first replication, but not the second*. • If it followed the semiconservative model one band would be seen after the first replication and 2 would be seen after the 2nd replication. ...
CIS 595 Bioinformatics
... transcription alone (sometimes referred to as the primary transcript) would contain both coding (exon) and noncoding (intron) sequences. Before it can be translated into protein, the two ends of the RNA are modified, the introns are removed by an enzymatically catalyzed RNA splicing reaction, and th ...
... transcription alone (sometimes referred to as the primary transcript) would contain both coding (exon) and noncoding (intron) sequences. Before it can be translated into protein, the two ends of the RNA are modified, the introns are removed by an enzymatically catalyzed RNA splicing reaction, and th ...
Protein Synthesis – Level 1
... 2. If the underlined portions represent introns, what will the mature mRNA be/read? 3. Prior to leaving the nucleus, what will be added to the mature mRNA? What will the mRNA look like after this occurs? What is the purpose of this processing? ...
... 2. If the underlined portions represent introns, what will the mature mRNA be/read? 3. Prior to leaving the nucleus, what will be added to the mature mRNA? What will the mRNA look like after this occurs? What is the purpose of this processing? ...
Casework Genetics Uses Illumina Technologies to Decipher
... High-throughput arrays enable forensic laboratories to unravel mixed samples and obtain high-quality results from severely degraded genetic material. Introduction The application of DNA technology to forensic testing has transformed the justice system, with DNA evidence now routinely used to solve c ...
... High-throughput arrays enable forensic laboratories to unravel mixed samples and obtain high-quality results from severely degraded genetic material. Introduction The application of DNA technology to forensic testing has transformed the justice system, with DNA evidence now routinely used to solve c ...
Question How does DNA control a cell?By controlling Protein
... transferred from the tRNA in the P-site to the tRNA in the A-site. ...
... transferred from the tRNA in the P-site to the tRNA in the A-site. ...
Biology 115 Lab 10:Gene Technology
... every time it sees the sequence GAATTC, while another might cut every time it sees ACTAGT. The fact that each endonuclease cuts at a specific sequence means that every time identical DNA is cut by one endonuclease, the cuts are at exactly the same place and the fragments of DNA produced are exactly ...
... every time it sees the sequence GAATTC, while another might cut every time it sees ACTAGT. The fact that each endonuclease cuts at a specific sequence means that every time identical DNA is cut by one endonuclease, the cuts are at exactly the same place and the fragments of DNA produced are exactly ...
A-level Biology B Question paper Unit 2 - Genes and Genetic
... This enzyme removes the adenine molecule from one of the nucleotides in the RNA of ribosomes. As a result, the ribosome changes shape. The diagram shows the nucleotide from which adenine is removed by ricin. ...
... This enzyme removes the adenine molecule from one of the nucleotides in the RNA of ribosomes. As a result, the ribosome changes shape. The diagram shows the nucleotide from which adenine is removed by ricin. ...
A new phagemid vector for positive selection of recombinants based
... control of the tac promoter and a vector fragment containing the ori and the ampicillin-resistance gene of the pUC19 plasmid. Uncut or re-ligated pMT440 does not support growth, whereas bacteria survive when transformed by plasmids containing inserts in the barnase gene, since the insertion of a for ...
... control of the tac promoter and a vector fragment containing the ori and the ampicillin-resistance gene of the pUC19 plasmid. Uncut or re-ligated pMT440 does not support growth, whereas bacteria survive when transformed by plasmids containing inserts in the barnase gene, since the insertion of a for ...
What is Genetic Modification?
... Livestock improvement using transgenic animals Transgenic sheep have been used to enhance wool production in flocks (above, left). The keratin protein of wool is largely made of a single amino acid, cysteine. Injecting developing sheep with the genes for the enzymes that generate cysteine produces w ...
... Livestock improvement using transgenic animals Transgenic sheep have been used to enhance wool production in flocks (above, left). The keratin protein of wool is largely made of a single amino acid, cysteine. Injecting developing sheep with the genes for the enzymes that generate cysteine produces w ...
Structure and function of DNA
... Explain why it is important that non-coding regions are removed from the primary transcript of this gene before translation. The wrong / extra amino acids are not placed in the protein / polypeptide formed OR so that the correct functional protein is formed ...
... Explain why it is important that non-coding regions are removed from the primary transcript of this gene before translation. The wrong / extra amino acids are not placed in the protein / polypeptide formed OR so that the correct functional protein is formed ...
File
... interpreted the photo and discovered the double helix structure (they won the nobel prize) CODON: group of _______ bases ___________: stretch of DNA that codes for a trait -the code is the order of the bases (______________) -genes are hundreds or thousands of bases long ...
... interpreted the photo and discovered the double helix structure (they won the nobel prize) CODON: group of _______ bases ___________: stretch of DNA that codes for a trait -the code is the order of the bases (______________) -genes are hundreds or thousands of bases long ...
DNA
... • The remarkable ability of bacteria to express some eukaryotic proteins underscores the shared evolutionary ancestry of living species • For example, Pax-6 is a gene that directs formation of a vertebrate eye; the same gene in flies directs the formation of an insect eye (which is quite different f ...
... • The remarkable ability of bacteria to express some eukaryotic proteins underscores the shared evolutionary ancestry of living species • For example, Pax-6 is a gene that directs formation of a vertebrate eye; the same gene in flies directs the formation of an insect eye (which is quite different f ...
CONTENTS DNA, RNA AND PROTEIN SYNTHESIS DNA
... Alternative splicing contributes to protein diversity − a single gene transcript (RNA) can have thousands of different splicing patterns, and will therefore code for thousands of different proteins: a diverse proteome is generated from a relatively limited genome. Splicing is important in genetic re ...
... Alternative splicing contributes to protein diversity − a single gene transcript (RNA) can have thousands of different splicing patterns, and will therefore code for thousands of different proteins: a diverse proteome is generated from a relatively limited genome. Splicing is important in genetic re ...
heredity (b)
... DNA replication is directional. In what direction does it occur? How does this differ for 5' -> 3' The 3' strand is leading because it is continuous, 5' lagging, discontinuous. the two strands? What enzyme is responsible for unwinding the supercoil? Topoisomerase What enzyme is responsible for unzip ...
... DNA replication is directional. In what direction does it occur? How does this differ for 5' -> 3' The 3' strand is leading because it is continuous, 5' lagging, discontinuous. the two strands? What enzyme is responsible for unwinding the supercoil? Topoisomerase What enzyme is responsible for unzip ...
Yeast DNA Prep (Quick) Formosa
... QUICK YEAST DNA PREP This technique is used for preparing genomic DNA for PCR, for recovering plasmids for transfer to E. coli, or for Southern Blots. These applications require different variations of the procedure, so the protocol diverges into three endings. 1. Grow yeast cultures to saturation o ...
... QUICK YEAST DNA PREP This technique is used for preparing genomic DNA for PCR, for recovering plasmids for transfer to E. coli, or for Southern Blots. These applications require different variations of the procedure, so the protocol diverges into three endings. 1. Grow yeast cultures to saturation o ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.