Mammalian two-hybrid (M2H) and co-immunoprecipitation (co
... plasmid (0.25ug) were co-transfected into HEK293 cells using lipofectamine 2000 at different concentration (0.01ug, 0.1ug, 0.5ug, respectively). pBD-p53 +pAD-SV40T+ pFR-Luc reporter plasmid were used for interaction positive control. pBD-p53 +pADTRAF+ pFR-Luc reporter plasmid were used for interacti ...
... plasmid (0.25ug) were co-transfected into HEK293 cells using lipofectamine 2000 at different concentration (0.01ug, 0.1ug, 0.5ug, respectively). pBD-p53 +pAD-SV40T+ pFR-Luc reporter plasmid were used for interaction positive control. pBD-p53 +pADTRAF+ pFR-Luc reporter plasmid were used for interacti ...
Class details
... Dye (Coomassie Brilliant Blue G-250) Dye binds protein, Abs increases (at 595nm) More protein = ? ...
... Dye (Coomassie Brilliant Blue G-250) Dye binds protein, Abs increases (at 595nm) More protein = ? ...
Lecture #2 - Suraj @ LUMS
... Bacteria contain plasmids, or small loops of DNA, that can be transmitted from one cell to another, either in the course of sex (yes, bacteria have sex) or by viruses. This ability to trade genes with all comers makes bacteria amazingly adaptible; beneficial genes, like those for antibiotic resistan ...
... Bacteria contain plasmids, or small loops of DNA, that can be transmitted from one cell to another, either in the course of sex (yes, bacteria have sex) or by viruses. This ability to trade genes with all comers makes bacteria amazingly adaptible; beneficial genes, like those for antibiotic resistan ...
Glossary of Key Terms in Chapter Two
... peptidyl tRNA binding site of ribosome (P-site) (20.6) a pocket on the surface of the ribosome that holds the tRNA bound to the growing peptide chain. point mutation (20.7) the substitution of a single base in a codon; this may or may not alter the genetic code of the mRNA resulting in the substitut ...
... peptidyl tRNA binding site of ribosome (P-site) (20.6) a pocket on the surface of the ribosome that holds the tRNA bound to the growing peptide chain. point mutation (20.7) the substitution of a single base in a codon; this may or may not alter the genetic code of the mRNA resulting in the substitut ...
Glossary of Key Terms in Chapter Two
... peptidyl tRNA binding site of ribosome (P-site) (20.6) a pocket on the surface of the ribosome that holds the tRNA bound to the growing peptide chain. point mutation (20.7) the substitution of a single base in a codon; this may or may not alter the genetic code of the mRNA resulting in the substitut ...
... peptidyl tRNA binding site of ribosome (P-site) (20.6) a pocket on the surface of the ribosome that holds the tRNA bound to the growing peptide chain. point mutation (20.7) the substitution of a single base in a codon; this may or may not alter the genetic code of the mRNA resulting in the substitut ...
Chapter 11
... • The effect of a mutation depends on the identity of the cell where it occurs. • Mutations in germ-line cells - will be passed to future generations • Important for evolutionary change • Mutations in somatic cells are not passed to future generations but passed to all other somatic cells derived fr ...
... • The effect of a mutation depends on the identity of the cell where it occurs. • Mutations in germ-line cells - will be passed to future generations • Important for evolutionary change • Mutations in somatic cells are not passed to future generations but passed to all other somatic cells derived fr ...
bacterial genetic
... gene mutation, gene replication, and gene recombination are much the same for all life forms. • The prototypic organism used in microbial genetic studies for the past fifty years is the enteric, gramnegative Escherichia coli ...
... gene mutation, gene replication, and gene recombination are much the same for all life forms. • The prototypic organism used in microbial genetic studies for the past fifty years is the enteric, gramnegative Escherichia coli ...
Viruses
... and destroy invading virus 2. Immune response – body detects foreign proteins called “antigens.” The body then produces antibodies to attack invader ...
... and destroy invading virus 2. Immune response – body detects foreign proteins called “antigens.” The body then produces antibodies to attack invader ...
Genetics - LLI Manassas
... the linear code of mRNA and opened DNA, to the threedimensional code of proteins. So, we’ve added a layer of complexity to the language, and we will see in the next section that DNA has 4-dimensions of communication. There certainly appears to be intelligence required for this process. Even the simp ...
... the linear code of mRNA and opened DNA, to the threedimensional code of proteins. So, we’ve added a layer of complexity to the language, and we will see in the next section that DNA has 4-dimensions of communication. There certainly appears to be intelligence required for this process. Even the simp ...
palm-print on stickers as a replacement of blood
... The DNA, which can be obtained from a 1.5 x 0.5 cm slice of a sticker, is of good quantity for PCR analysis in most cases. However, depending on the conditions of the hand, which are still not clear to us, some palm-prints of the same person taken on different days gave poor results. This problem is ...
... The DNA, which can be obtained from a 1.5 x 0.5 cm slice of a sticker, is of good quantity for PCR analysis in most cases. However, depending on the conditions of the hand, which are still not clear to us, some palm-prints of the same person taken on different days gave poor results. This problem is ...
DNA Structure and Replication
... • Coins the term Nucleic Acid – because it was found in the Nucleus of the cell • Most people thought proteins were hereditary material, so no one cared. ...
... • Coins the term Nucleic Acid – because it was found in the Nucleus of the cell • Most people thought proteins were hereditary material, so no one cared. ...
dna-and-protein-synthesis-blog-post
... DNA and Protein Synthesis DNA Model 1. Explain the structure of DNA – use the terms nucleotides, antiparallel strands, and complimentary base pairing. Deoxyribonucleic Acid (DNA), is a large molecule that controls cells activities and has the instructions on how to build proteins. The monomers of nu ...
... DNA and Protein Synthesis DNA Model 1. Explain the structure of DNA – use the terms nucleotides, antiparallel strands, and complimentary base pairing. Deoxyribonucleic Acid (DNA), is a large molecule that controls cells activities and has the instructions on how to build proteins. The monomers of nu ...
+ + מורן גרינברג 2008
... • DNA Polymerase is the enzyme responsible for copying the sequence starting at the primer from the single DNA strand • Commonly use Taq, an enzyme from the hyperthermophilic organisms Thermus aquaticus, isolated first at a thermal spring in Yellowstone National Park • This enzyme is heat-tolerant ...
... • DNA Polymerase is the enzyme responsible for copying the sequence starting at the primer from the single DNA strand • Commonly use Taq, an enzyme from the hyperthermophilic organisms Thermus aquaticus, isolated first at a thermal spring in Yellowstone National Park • This enzyme is heat-tolerant ...
Viruses
... • non-cellular; do not respire, grow/ develop, respond to stimuli • Parasitic on living cells - nucleic material in viruses give information for production of new viruses • viruses are spread from infected cells by direct contact or indirectly through air, water, food, fecal material ...
... • non-cellular; do not respire, grow/ develop, respond to stimuli • Parasitic on living cells - nucleic material in viruses give information for production of new viruses • viruses are spread from infected cells by direct contact or indirectly through air, water, food, fecal material ...
Minimizing Observer Effects in the Interpretation of Forensic DNA
... allow sensitive evaluation of population structure. Databases that have been made available for such analyses at the present time are relatively small collections of hundreds of individuals while NDIS presently contains more than 10,000,000 complete 13-locus STR DNA profiles. The relative utility of ...
... allow sensitive evaluation of population structure. Databases that have been made available for such analyses at the present time are relatively small collections of hundreds of individuals while NDIS presently contains more than 10,000,000 complete 13-locus STR DNA profiles. The relative utility of ...
Stress protein synthesis: EMF interaction with DNA
... stress protein gene shows two different DNA segments in the gene promoter, one for a specific EMF response to low energy stimuli, and another for high energy thermal stimuli. The EMF-specific DNA segments can be activated by EMF when they are coupled to other genes. Studies of enzyme reactions sho ...
... stress protein gene shows two different DNA segments in the gene promoter, one for a specific EMF response to low energy stimuli, and another for high energy thermal stimuli. The EMF-specific DNA segments can be activated by EMF when they are coupled to other genes. Studies of enzyme reactions sho ...
sample exam 2010
... 76. Discuss the importance of 4 intermolecular and intramolecular forces in the secondary and tertiary structure of proteins. Name two ways these forces can be disrupted causing denaturation of the protein. (6 marks) 77. Describe the main idea behind the induced-fit model of enzyme activity. (2 mark ...
... 76. Discuss the importance of 4 intermolecular and intramolecular forces in the secondary and tertiary structure of proteins. Name two ways these forces can be disrupted causing denaturation of the protein. (6 marks) 77. Describe the main idea behind the induced-fit model of enzyme activity. (2 mark ...
1. Which of the following is an example of a hydrogen bond
... Achondroplastic dwarfism is a dominant genetic trait that causes severe malformation of the skeleton. Homozygotes for this condition are spontaneously aborted (hence, the homozygous condition is lethal) but heterozygotes will develop to be dwarfed. Matthew has a family history of the condition, alth ...
... Achondroplastic dwarfism is a dominant genetic trait that causes severe malformation of the skeleton. Homozygotes for this condition are spontaneously aborted (hence, the homozygous condition is lethal) but heterozygotes will develop to be dwarfed. Matthew has a family history of the condition, alth ...
1 Taxonomy
... • Genomics: the study of an organisms genes; used to classify a microorganisms. • Bio remediation: bacteria degrade organic matter in sewage. Bacteria also degrade or detoxify pollutants such as oil and mercury. • Genetic engineering: a new technique for biotechnology. Bacteria and fungi can produc ...
... • Genomics: the study of an organisms genes; used to classify a microorganisms. • Bio remediation: bacteria degrade organic matter in sewage. Bacteria also degrade or detoxify pollutants such as oil and mercury. • Genetic engineering: a new technique for biotechnology. Bacteria and fungi can produc ...
Chapter 8 The World of Microbes
... Heterotrophs – when nutrients are derived from another organism ¾ Parasites – if taken from living organisms. Most diseasecausing bacteria use this. ¾ Saprophytes – if taken from dead and decaying organisms Autotrophs – when nutrients are made by the bacteria. ⇒ Photosynthesis – when light energy i ...
... Heterotrophs – when nutrients are derived from another organism ¾ Parasites – if taken from living organisms. Most diseasecausing bacteria use this. ¾ Saprophytes – if taken from dead and decaying organisms Autotrophs – when nutrients are made by the bacteria. ⇒ Photosynthesis – when light energy i ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".