Detection of Free Radicals
... “Premarin (Wyeth–Ayerst) is the most common drug used for hormone replacement therapy (HRT) and is composed of approximately 50% estrogens and 40% equine estrogens [equilenin (EN) and equilin (EQ)] (9). In vitro experiments have shown that equine estrogens are successively metabolized and are capabl ...
... “Premarin (Wyeth–Ayerst) is the most common drug used for hormone replacement therapy (HRT) and is composed of approximately 50% estrogens and 40% equine estrogens [equilenin (EN) and equilin (EQ)] (9). In vitro experiments have shown that equine estrogens are successively metabolized and are capabl ...
(January 2005).
... A. Gene must be identified; B. Using e.g. gene probes/nucleotide/amino acid sequence; C. Gene is cut out/isolated using restriction enzymes; (not: extracted) D. or made from RNA using reverse transcriptase; E. ...
... A. Gene must be identified; B. Using e.g. gene probes/nucleotide/amino acid sequence; C. Gene is cut out/isolated using restriction enzymes; (not: extracted) D. or made from RNA using reverse transcriptase; E. ...
The sequence of the tms transcript 2 locus of the A. tumefaciens
... the formation of shooting tumors on various host plants. Indeed, when the pTiA66 T-DNA region was analyzed, it was found to have a 2.7 Kb insert that mapped to the region of the transcript 2 sequences (16). As a step towards understanding the role(s) of transcript 2 in crown gall tumor formation and ...
... the formation of shooting tumors on various host plants. Indeed, when the pTiA66 T-DNA region was analyzed, it was found to have a 2.7 Kb insert that mapped to the region of the transcript 2 sequences (16). As a step towards understanding the role(s) of transcript 2 in crown gall tumor formation and ...
PHM 281N Pharmaceutical Biochemistry II
... If there is a disagreement over the answer to a specific question, the student should present his/her exam plus a written explanation (with appropriate documentation) to the instructor within 72 hours of the listserv announcement of the posting of exam results & key as described above. Documentation ...
... If there is a disagreement over the answer to a specific question, the student should present his/her exam plus a written explanation (with appropriate documentation) to the instructor within 72 hours of the listserv announcement of the posting of exam results & key as described above. Documentation ...
Production of bacterial cellulose by agitation culture
... pathway blocked to decrease the amounts of by-products (refs.8,9). We bred mutant cells whose growth was increased to increase the enhancement of BC productivity (ref.10). Several host-vector systems for BC producing bacteria were already available and some of these use broad host range plasmids, su ...
... pathway blocked to decrease the amounts of by-products (refs.8,9). We bred mutant cells whose growth was increased to increase the enhancement of BC productivity (ref.10). Several host-vector systems for BC producing bacteria were already available and some of these use broad host range plasmids, su ...
RNA Polymerase II Subunit Rpb9 Regulates Transcription
... which is a phenotype linked to defects in transcription elongation. Expression of the TFIIS gene on a high-copy plasmid partially suppressed the 6-azauracil sensitivity of ⌬rpb9 cells. We set out to determine the relevant cellular role of yeast Rpb9 by assessing the ability of 20 different site-dire ...
... which is a phenotype linked to defects in transcription elongation. Expression of the TFIIS gene on a high-copy plasmid partially suppressed the 6-azauracil sensitivity of ⌬rpb9 cells. We set out to determine the relevant cellular role of yeast Rpb9 by assessing the ability of 20 different site-dire ...
Isolation of a Complementary DNA Clone for the Human
... for genes within the MHC have permitted an analysis of the fine structure of this region (13, 19). Additional structural data derived from nucleotide sequencing will lead to an understanding of the molecular basis of polymorphic variants important in regulation of the immune response. DNA polymorphi ...
... for genes within the MHC have permitted an analysis of the fine structure of this region (13, 19). Additional structural data derived from nucleotide sequencing will lead to an understanding of the molecular basis of polymorphic variants important in regulation of the immune response. DNA polymorphi ...
Fusobacterium pseudonecrophorurn Is a Synonym for Fusobacten
... M NaCl containing 5 mM HEPES (N-2-hydroxyethylpiperazine-N'-Zethanesulfonic acid) buffer (pH 7.0), and 25 p1 of deionized formamide. These vials were incubated at 50°C for 20 h. The S1 nuclease procedure was used for DNA similarity determinations (8). Labeled reference DNA preparations reassociated ...
... M NaCl containing 5 mM HEPES (N-2-hydroxyethylpiperazine-N'-Zethanesulfonic acid) buffer (pH 7.0), and 25 p1 of deionized formamide. These vials were incubated at 50°C for 20 h. The S1 nuclease procedure was used for DNA similarity determinations (8). Labeled reference DNA preparations reassociated ...
PROTEIN SYNTHESIS
... actin and myosin. Transport proteins are responsible for carrying many materials through the circulatory system. Haemoglobin transports oxygen and carbon dioxide in the blood. Interaction of a number of different proteins results in the clotting of blood. Antibodies can recognize and inactivate virt ...
... actin and myosin. Transport proteins are responsible for carrying many materials through the circulatory system. Haemoglobin transports oxygen and carbon dioxide in the blood. Interaction of a number of different proteins results in the clotting of blood. Antibodies can recognize and inactivate virt ...
dna biometrics - Danish Biometrics
... “Biometrics” is a term used in many aspects of our world. It is no longer a term known only to the government or “secret research organizations”. Biometrics is characterized by physical features of a being and measurement of those features. When the general public hears biometrics, the first thought ...
... “Biometrics” is a term used in many aspects of our world. It is no longer a term known only to the government or “secret research organizations”. Biometrics is characterized by physical features of a being and measurement of those features. When the general public hears biometrics, the first thought ...
Comparison of real-time PCR with SYBR Green I or
... rDNA of the selected target bacteria. The Bacteroides fragilis and Bifidobacterium lactis assays were designed to be species-specific, whereas the remaining four assays were set to detect closely related bacterial species. According to the sequence analyses, the Bifidobacterium longum assay detected ...
... rDNA of the selected target bacteria. The Bacteroides fragilis and Bifidobacterium lactis assays were designed to be species-specific, whereas the remaining four assays were set to detect closely related bacterial species. According to the sequence analyses, the Bifidobacterium longum assay detected ...
DNA and RNA:
... in terms of the odds that a given trait would be passed on to an individual in the next generation. This “probability” model of genetics left some questions unanswered: • What is the nature of the genetic information? • How does the cell use the genetic information? As is often the case in science, ...
... in terms of the odds that a given trait would be passed on to an individual in the next generation. This “probability” model of genetics left some questions unanswered: • What is the nature of the genetic information? • How does the cell use the genetic information? As is often the case in science, ...
How Scientists Think
... being in a particular shape; thus, the level of enzyme activity could be used to monitor the degree to which ribonuclease protein successfully achieved the proper catalytic shape. To watch the folding process, one might start with nascent proteins, newly made and not yet folded, or one might choose ...
... being in a particular shape; thus, the level of enzyme activity could be used to monitor the degree to which ribonuclease protein successfully achieved the proper catalytic shape. To watch the folding process, one might start with nascent proteins, newly made and not yet folded, or one might choose ...
Chapter 1
... Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
... Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
Candidatus Liberibacter asiaticus - Southwest Florida Research and
... primer set, except that both the annealing and extension temperatures were at 72°C. The conventional PCR and Q-PCR (see below) amplifications were repeated with all the samples at least three to five times, and the representation of one experiment is shown in Figure 1. Q-PCR. Q-PCR was carried out w ...
... primer set, except that both the annealing and extension temperatures were at 72°C. The conventional PCR and Q-PCR (see below) amplifications were repeated with all the samples at least three to five times, and the representation of one experiment is shown in Figure 1. Q-PCR. Q-PCR was carried out w ...
Chromium Incorporated in RNA and DNA
... were significantly greater than bound to RNA and was 5.12 ð 0.30/1000 and 1.80 ð 0.11/1000 nucleotides for 10 µm potassium dichromate or chromium chloride and 7.74 ð 0.44/1000 nucleotides and 3.70 ð 0.25 for 100 µm respectively. Under the same conditions to RNA 3.86 ð 0.45 Cr atoms/ 1000 nucleotides ...
... were significantly greater than bound to RNA and was 5.12 ð 0.30/1000 and 1.80 ð 0.11/1000 nucleotides for 10 µm potassium dichromate or chromium chloride and 7.74 ð 0.44/1000 nucleotides and 3.70 ð 0.25 for 100 µm respectively. Under the same conditions to RNA 3.86 ð 0.45 Cr atoms/ 1000 nucleotides ...
A novel procedure for genotyping of single nucleotide polymorphisms in trisomy with genomic DNA and the invader assay.
... can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an InvaderÕ assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and ...
... can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an InvaderÕ assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and ...
E coli fact sheet - Village of Michiana
... Since E. coli is found in human and animal waste, it is often used as an indicator to detect ...
... Since E. coli is found in human and animal waste, it is often used as an indicator to detect ...
Bone Marrow Failure Associated with Herpesvirus 8 Infection in a
... and harmful complications. Multiple concomitant viral infections might also enhance the lytic-cycle spread of emerging infectious pathogens, such as HHV-8. This is a g-herpesvirus that has been shown by genomic sequence analysis to be closely related to herpesvirus saimiri and rhesus monkey rhadinov ...
... and harmful complications. Multiple concomitant viral infections might also enhance the lytic-cycle spread of emerging infectious pathogens, such as HHV-8. This is a g-herpesvirus that has been shown by genomic sequence analysis to be closely related to herpesvirus saimiri and rhesus monkey rhadinov ...
Nucleotide sequence and structural organization of
... reading frames (ORFs). ORF 1was capable of encoding a 249 kDa peptide which shared homology with the replication initiation protein (RepB) from a number of Gram-positive rolling circle plasmids. ORF 2 could encode a peptide of 6-6 kDa which was homologous to the RepC protein of the lactococcal plasm ...
... reading frames (ORFs). ORF 1was capable of encoding a 249 kDa peptide which shared homology with the replication initiation protein (RepB) from a number of Gram-positive rolling circle plasmids. ORF 2 could encode a peptide of 6-6 kDa which was homologous to the RepC protein of the lactococcal plasm ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".