Metalloenzyme Functions
... We call this the E-value for the score S. This formula makes eminently intuitive sense. Doubling the length of either sequence should double the number of HSPs attaining a given score. Also, for an HSP to attain the score 2x it must attain the score x twice in a row, so one expects E to decrease exp ...
... We call this the E-value for the score S. This formula makes eminently intuitive sense. Doubling the length of either sequence should double the number of HSPs attaining a given score. Also, for an HSP to attain the score 2x it must attain the score x twice in a row, so one expects E to decrease exp ...
Molecular Phylogenetics Basis of Phylogenetics
... ¾ a phylogeny describe the historical relationships among lineages or organisms or their parts (genes) ¾ using trees to test hypotheses ...
... ¾ a phylogeny describe the historical relationships among lineages or organisms or their parts (genes) ¾ using trees to test hypotheses ...
Chapter 7 Operons: Fine Control of Bacterial Transcription Bacterial
... • Measure protein binding to [γ32P]-DNA using native PAG: • Bent DNA runs slower than linear; • Use fragments with CAP binding site (red) in different positions relative to restriction enzyme sites • See which fragment runs Fig. 18; experimental agreed with predictions slowest ...
... • Measure protein binding to [γ32P]-DNA using native PAG: • Bent DNA runs slower than linear; • Use fragments with CAP binding site (red) in different positions relative to restriction enzyme sites • See which fragment runs Fig. 18; experimental agreed with predictions slowest ...
The Origins of Ecological Diversity in Prokaryotes
... groups have been refractory to discovery: only a minute fraction of prokaryotes are at present cultivable [2], hindering laboratory study of physiology, and morphology is not a reliable indicator of prokaryotic diversity. Ecologists and systematists have worked around these difficulties through mole ...
... groups have been refractory to discovery: only a minute fraction of prokaryotes are at present cultivable [2], hindering laboratory study of physiology, and morphology is not a reliable indicator of prokaryotic diversity. Ecologists and systematists have worked around these difficulties through mole ...
in Power-Point Format
... • Measure protein binding to [g32P]-DNA using native PAG: • Bent DNA runs slower than linear; • Use fragments with CAP binding site (red) in different positions relative to restriction enzyme sites • See which fragment runs slowest Fig. 18; experimental agreed with predictions ...
... • Measure protein binding to [g32P]-DNA using native PAG: • Bent DNA runs slower than linear; • Use fragments with CAP binding site (red) in different positions relative to restriction enzyme sites • See which fragment runs slowest Fig. 18; experimental agreed with predictions ...
Fungal soil communities in a young transgenic poplar plantation
... reads shorter than 150 bp and reads containing more than four ambiguity symbols. On average, 74% of all reads passed these criteria. The individual sample FASTA files were subjected to cluster analysis for a tentative OTU count using the clustering function of USEARCH v5.3.23 (Edgar 2010) with the f ...
... reads shorter than 150 bp and reads containing more than four ambiguity symbols. On average, 74% of all reads passed these criteria. The individual sample FASTA files were subjected to cluster analysis for a tentative OTU count using the clustering function of USEARCH v5.3.23 (Edgar 2010) with the f ...
Arg 41 - Saudi Medical Journal
... two carriers (III:1, III:2) were available for this study. Thus, peripheral blood samples were collected from 5 members of a family in an EDTA-containing tubes. Qiagen Mini Genomic DNA Extraction Kit was used to isolate Genomic DNA following manufacturer's instructions. Polymerase chain reaction (PC ...
... two carriers (III:1, III:2) were available for this study. Thus, peripheral blood samples were collected from 5 members of a family in an EDTA-containing tubes. Qiagen Mini Genomic DNA Extraction Kit was used to isolate Genomic DNA following manufacturer's instructions. Polymerase chain reaction (PC ...
BMC Genomics
... plant cell-wall degrading enzymes, and high salinity tolerance. Moreover, the presence or absence of bacterial growth at 37°C was identified as a very simple method in differentiating between pathogenic and non-pathogenic isolates. DSM14405T is not able to grow at this human-relevant temperature, mo ...
... plant cell-wall degrading enzymes, and high salinity tolerance. Moreover, the presence or absence of bacterial growth at 37°C was identified as a very simple method in differentiating between pathogenic and non-pathogenic isolates. DSM14405T is not able to grow at this human-relevant temperature, mo ...
Detection of GM Papaya Event 55-1 in Fresh
... Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene ...
... Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene ...
Incorporation of reporter molecule
... designated a cassette (Cass) consisting of the four natural bases (dG/dA/dT/dC). Therefore, for each given cassette template, the template number de®nes the number of cassette sequences encoded by that template, e.g. Cass 1 had one cassette, i.e. four bases (CGTA), while Cass 10 contained 10 cassett ...
... designated a cassette (Cass) consisting of the four natural bases (dG/dA/dT/dC). Therefore, for each given cassette template, the template number de®nes the number of cassette sequences encoded by that template, e.g. Cass 1 had one cassette, i.e. four bases (CGTA), while Cass 10 contained 10 cassett ...
A new subgroup of the IS3 family and properties of its
... 1a). However, some features of ISPpy1 distinguish it from other IS3-family elements (Table 1) (Petrova et al., 2012). To obtain further insight into the distribution of ISPpy1 in natural bacterial populations, we attempted to identify additional ISPpy1 copies in bacterial strains available in our co ...
... 1a). However, some features of ISPpy1 distinguish it from other IS3-family elements (Table 1) (Petrova et al., 2012). To obtain further insight into the distribution of ISPpy1 in natural bacterial populations, we attempted to identify additional ISPpy1 copies in bacterial strains available in our co ...
Counting Small RNA in Pathogenic Bacteria
... of probes to a sRNA target, the design rules of ∼50% G-C content, large separation between individual probes, and avoiding repetitive sequences must be substantially relaxed, which leads to higher nonspecific binding and lower hybridization efficiencies. In particular, for both sRNA targets investigate ...
... of probes to a sRNA target, the design rules of ∼50% G-C content, large separation between individual probes, and avoiding repetitive sequences must be substantially relaxed, which leads to higher nonspecific binding and lower hybridization efficiencies. In particular, for both sRNA targets investigate ...
Molecular Biology
... 3. Empty and clean the sample cuvette and add the diluted RNA. 4. Record absorbance of RNA sample at both 260 and 280 nm. Correct the readings as necessary using the blank values you determined in step 1. 5. The absorbance at 260 nm allows calculation of the concentration of DNA or RNA in the sample ...
... 3. Empty and clean the sample cuvette and add the diluted RNA. 4. Record absorbance of RNA sample at both 260 and 280 nm. Correct the readings as necessary using the blank values you determined in step 1. 5. The absorbance at 260 nm allows calculation of the concentration of DNA or RNA in the sample ...
PDF - Scient Open Access
... nm (A490) using ELISA reader. Optical density cut-off (ODc) was determined. It is defined as average OD of negative control + 3× standard deviation (SD) of negative control. Formation of biofilm by isolates was analyzed and categorized relying on the absorbance of the safranin-stained attached cells ...
... nm (A490) using ELISA reader. Optical density cut-off (ODc) was determined. It is defined as average OD of negative control + 3× standard deviation (SD) of negative control. Formation of biofilm by isolates was analyzed and categorized relying on the absorbance of the safranin-stained attached cells ...
Structure of DIG
... DIG-dUTP is used as a dTTP analog, gets incorporated by DNA polymerase in place of T bases DIG ...
... DIG-dUTP is used as a dTTP analog, gets incorporated by DNA polymerase in place of T bases DIG ...
Coupling spatial segregation with synthetic circuits to control
... Keasling, 2008; Ruder et al, 2011; Weber & Fussenegger, 2012). To date, efforts in synthetic biology have focused on engineering of circuit-centric or cell-centric functions (Endy, 2005; Brophy & Voigt, 2014; Pardee et al, 2014). When engineering a circuit, the host cell is typically considered a st ...
... Keasling, 2008; Ruder et al, 2011; Weber & Fussenegger, 2012). To date, efforts in synthetic biology have focused on engineering of circuit-centric or cell-centric functions (Endy, 2005; Brophy & Voigt, 2014; Pardee et al, 2014). When engineering a circuit, the host cell is typically considered a st ...
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
... appropriate antimicrobial treatment remains the cornerstone in the management of patients and helps in improving the outcome of the disease. Cerebrospinal fluid (CSF) culture is considered to be gold standard for diagnosis of bacterial meningitis, but it is time consuming (24-48 hours) and can give ...
... appropriate antimicrobial treatment remains the cornerstone in the management of patients and helps in improving the outcome of the disease. Cerebrospinal fluid (CSF) culture is considered to be gold standard for diagnosis of bacterial meningitis, but it is time consuming (24-48 hours) and can give ...
Chapter 14 - People Server at UNCW
... Copyright © McGraw-Hill Education. Permission required for reproduction or display. ...
... Copyright © McGraw-Hill Education. Permission required for reproduction or display. ...
evolution of protein function by domain swapping
... Protein remodeling by rational design utilizes our current understanding of how proteins function and, guided by computer modeling, identifies specific changes designed to impart the desired function onto the existing protein. Since our current understanding of enzymes is less than complete, combina ...
... Protein remodeling by rational design utilizes our current understanding of how proteins function and, guided by computer modeling, identifies specific changes designed to impart the desired function onto the existing protein. Since our current understanding of enzymes is less than complete, combina ...
THE EFFECT OF R-FACTOR CARRIAGE ON THE SURVIVAL O F
... THEintroduction of most antibiotics has been initially followed by reports of a progressively increasing population of resistant organisms (Finland, 1972). Since the discovery of antibiotic-resistance transfer (R) factors (Watanabe, 1963) a high proportion of antibiotic resistance in the Enterobacte ...
... THEintroduction of most antibiotics has been initially followed by reports of a progressively increasing population of resistant organisms (Finland, 1972). Since the discovery of antibiotic-resistance transfer (R) factors (Watanabe, 1963) a high proportion of antibiotic resistance in the Enterobacte ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... was added to the cell pellet to a final volume of 50 mL. The suspension was mixed gently by inverting, set on ice for 15 minutes, and then centrifuged for 15 minutes at 1,800g and 4°C. The supernatant was discarded, and the pellet resuspended in fresh lysis buffer to a final volume of 50 mL. The sus ...
... was added to the cell pellet to a final volume of 50 mL. The suspension was mixed gently by inverting, set on ice for 15 minutes, and then centrifuged for 15 minutes at 1,800g and 4°C. The supernatant was discarded, and the pellet resuspended in fresh lysis buffer to a final volume of 50 mL. The sus ...
Variations in amino acid composition in bacterial single stranded
... known as OB-fold (a structurally conserved folding motif) (8). The OB-fold binds to ssDNA through a combination of electrostatic and base-stacking interactions with the phosphodiester backbone and nucleotide bases (9). It comprises five antiparallel b-sheets which form a b-barrel capped by an a-heli ...
... known as OB-fold (a structurally conserved folding motif) (8). The OB-fold binds to ssDNA through a combination of electrostatic and base-stacking interactions with the phosphodiester backbone and nucleotide bases (9). It comprises five antiparallel b-sheets which form a b-barrel capped by an a-heli ...
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... The wondrous variety of colours displayed by flowers is due to four structurally distinct classes of pigments, namely flavonoids, carotenoids, betalains and chlorophyll. Chlorophyll is very rare as a flower pigment, and is only present in the handful of flowers that show a slight green hue (Eugster ...
... The wondrous variety of colours displayed by flowers is due to four structurally distinct classes of pigments, namely flavonoids, carotenoids, betalains and chlorophyll. Chlorophyll is very rare as a flower pigment, and is only present in the handful of flowers that show a slight green hue (Eugster ...
The Effects of selected toothpaste on the microbial flora of the mouth
... the growth of unwanted microbes in the surrounding. All these were executed under strict best sterile condition (i.e presence of Bunsen flame), possible. The media were allowed to set, after which they were dried in an incubator for 10 minutes to remove condensed water. Nutrient Broth Nutrient broth ...
... the growth of unwanted microbes in the surrounding. All these were executed under strict best sterile condition (i.e presence of Bunsen flame), possible. The media were allowed to set, after which they were dried in an incubator for 10 minutes to remove condensed water. Nutrient Broth Nutrient broth ...
Peay et al 2008 - North American Mycoflora Project
... A number of excellent reviews have examined the current array of molecular techniques available to ecologists interested in working with fungi (Horton and Bruns 2001, Anderson and Cairney 2004, Bidartondo and Gardes 2005). The intention of this overview is not to add to this growing list, but instea ...
... A number of excellent reviews have examined the current array of molecular techniques available to ecologists interested in working with fungi (Horton and Bruns 2001, Anderson and Cairney 2004, Bidartondo and Gardes 2005). The intention of this overview is not to add to this growing list, but instea ...