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Genomes
Genomes

... Multicellular eukaryotes have much noncoding DNA and many multigene families  The bulk of most eukaryotic genomes neither encodes proteins nor functional RNAs  Much evidence indicates that noncoding DNA (previously called “junk DNA”) plays important roles in the cell  For example, genomes of huma ...
Image PowerPoint
Image PowerPoint

... many proteins in the hemoglobin molecule. b) Sickle cell disease. A single base change in DNA codes via RNA for a different amino acid, valine. But this critical amino acid is important in proper folding of the hemoglobin molecule, which becomes defective, producing sickled red blood cells. ...
PURINE COMPOUNDS Both the pyrimidine bases (uracil, cytosine), and
PURINE COMPOUNDS Both the pyrimidine bases (uracil, cytosine), and

... Both the pyrimidine bases (uracil, cytosine), and the purine bases (adenine, guanine) are building blocks in the synthesis of DNA and RNA nucleotides. In the replication process, nucleotides are joined to one another to form DNA strands. It is less clear how the purine antagonists function, but they ...
The Nature of Genes The Nature of Genes
The Nature of Genes The Nature of Genes

... • Introns are spliced out of pre-mRNAs to produce the mature mRNA that is translated. • Alternative splicing recognizes different splice sites in different tissue types. • The mature mRNAs in each tissue possess different exons, resulting in different polypeptide products from the same gene. ...
Gene regulation in bacteria -
Gene regulation in bacteria -

... there is a group of five genes in E. coli encoding enzymes that are needed for synthesising the amino acid tryptophan. A grouping like this is called an ‘operon’. There is a similar group of three genes encoding enzymes that are needed to break down the sugar arabinose and another group of three gen ...
Tracing Phylogeny
Tracing Phylogeny

The human genome of is found where in the human body?
The human genome of is found where in the human body?

... means 6 million differences ...
Cons
Cons

... food activists, argue that …residents have a right to know what's in their food. Organic farmers, advocacy groups ...
L2_Cloning vectors
L2_Cloning vectors

DOC
DOC

... that is used to decode its genetic code and use the information to make proteins. Genes are made of DNA. The expression of DNA is protein. The term given for making a protein is called “protein synthesis.” This requires DNA to provide the coded genetic information, the three types of RNA, and the am ...
Utilization of FIA-UV/ED for detection of adenine derivates
Utilization of FIA-UV/ED for detection of adenine derivates

... Republic. *Tel: +420 545 133 350, Fax: +420 545 212 044 E-mail: [email protected] ...
Poster Abstract for the ICES Annual Science Conference
Poster Abstract for the ICES Annual Science Conference

... indicate that a significant part of the mackerel stock has entered the Icelandic EEZ during its summer feeding migration since then (~1.5 million tons in 2012 and 2013, 1.6 million tons in 2014 and 2.9 million tons in 2015) where they increase in weight by ~43%. This suggests that mackerel feeding i ...
IPTG_09-10_8h
IPTG_09-10_8h

... when multiple types of sugar are present in the growth media. Today, we are going to focus on one of the most well known examples—the lac operon. The lac operon controls the production of three different genes associated with the metabolism of lactose. One of these genes is lacZ, which encodes for t ...
A national scientific reference site network within the Terrestrial
A national scientific reference site network within the Terrestrial

Sequencing the Human Genome
Sequencing the Human Genome

... different ddNTP fluorescence colors are then automatically detected and the fluorescence intensity translated into a data “peak” that represents the order of nucleotides in the template DNA (Figure 1D). Sanger sequencing was introduced in 1977 and was the major method used to create the first genome se ...
END OF SEMESTER EXAM PREPARATION AND REVISION
END OF SEMESTER EXAM PREPARATION AND REVISION

... • Parent strands of DNA double helix serve as templates • Helicase unwinds the double helix and two daughter strands are formed • Each new DNA contains one parent and one daughter strand • Leading strand: 5’ –> 3’ (continuous) • Lagging strand: Okazaki fragments to form daughter strand (disconti ...
Metagenomic analysis of a soil enrichment cultures
Metagenomic analysis of a soil enrichment cultures

... 16s rRNA gene of virtually all species because they hybridize to a highly conserved region. However, an important difference here is that the template is a pool of mixed genomic DNA, so there are potentially 1000s of different 16s rRNA genes present. Each one is amplified in the reaction, yielding a ...
RNA - Humble ISD
RNA - Humble ISD

... and then throw parts away? May allow same gene to be used in different ways in different kinds of cells. May have a role in evolution… allows small changes in genes to have a big effect. ...
Setting up a transformation--how will the competent cells be treated?
Setting up a transformation--how will the competent cells be treated?

... Selecting recombinant lambda phages II • Wild type lambda cannot grow on E. coli infected with phage P2 (spi, or sensitive to P2 inhibition), spi+ conferred by red and gam genes in “stuffer” • Only phage lacking stuffer (they don’t have spi gene) can make plaques on lawn of E. coli containing a P2 ...
Unit 8 - Macromolecules Processes
Unit 8 - Macromolecules Processes

... the bonds between bases • Complementary base pairs are added by DNA Polymerase • Produces 2 new DNA molecules each with one original strand and one new strand ...
Genes Lost and Genes Found: Evolution of Bacterial Pathogenesis
Genes Lost and Genes Found: Evolution of Bacterial Pathogenesis

... avirulent strain, and in the second, genes ...
Mutations
Mutations

... Remove and replace incorrect bases (can be done at any time in cell cycle) ...
The early history of the genetics of photosynthetic bacteria: a
The early history of the genetics of photosynthetic bacteria: a

... figured that, since they discovered phages first, phages must have evolved first. In fact, it is most difficult to resolve this issue experimentally, and it remains unresolved till today (Lang and Beatty 2001). So now there was a genetic exchange system for R. capsulatus, and we could begin analyzin ...
15 Biodiversity in Ecosystems Experiment PRELIMINARY ACTIVITY FOR
15 Biodiversity in Ecosystems Experiment PRELIMINARY ACTIVITY FOR

... reasonably accurate? Since many animals and birds move in and out of an area, over what time period will you count organisms?  How will we identify a plant patch? A patch of one type of plant is an area of similar plants that are physically separate from another area of similar plants. Patches need ...
Discovery of DNA
Discovery of DNA

... destroyed its DNA ○ Rough bacteria did not become deadly ● Conclusion: DNA stores the genetic material in a cell ...
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