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Protein Synthesis
Protein Synthesis

...  Polypeptide is modified in the rough ER – this might include cutting out sections and/or cut a section from one part of the polypeptide and moving it to another part  Chaperone proteins help to fold the polypeptide into its final tertiary shape. Now it is called a protein. ...
[i,j].
[i,j].

... *The strength of a bond can be measured by the energy required to break it, here given in kilocalories per mole (kcal/mole). (One kilocalorie is the quantity of energy needed to raise the temperature of 1000 g of water by 1°C. An alternative unit in wide use is the kilojoule, kJ, equal to 0.24 kcal. ...
CS 753 BIOLOGY AND BIOCHEMISTRY
CS 753 BIOLOGY AND BIOCHEMISTRY

... 1. Soil fumigation method - A sample of soil is sieved and placed in a container and CO2 output measured over 20 days. There is typically a period of very rapid respiration followed by a much lower but stable respiration rate. If the organisms are killed by fumigation with chloroform, this initial f ...
Parts Washing Using ChemFree Bioremediation Technology
Parts Washing Using ChemFree Bioremediation Technology

... No EPA or OSHA paperwork ...
Proteomics
Proteomics

ACIDITY (free fatty acid) | fat matrix
ACIDITY (free fatty acid) | fat matrix

... the diluent. Mix the oil with diluent, after adding, by inverting test cuvette. Use 2,5 uL of diluted sample for testing.   **Application method for curve Acid. dil ‐ 50: Take 50 µL of oil, using the specific pipette (see Note 1) and add it to  the diluent. Mix the oil with diluent, after adding, by ...
100 Pectin is a complex polysaccharide consisting mainly of
100 Pectin is a complex polysaccharide consisting mainly of

Sequencing genomes
Sequencing genomes

... And the same is true for Dayhoff’s model of evolution. If we need to obtain probability matrices for higher percentage of accepted mutations (i.e. covering longer evolutionary time), we do matrix powers. Let’s say we want PAM120 – 120 mutations fixed on average per 100 residues. We do PAM1120. ...
BBF RFC 39: The USER cloning standard
BBF RFC 39: The USER cloning standard

... at 25°C) results in the cloning procedures to be completed in a very short time compared to standard cloning procedures. The USER technique is in many instances superior to traditional cloning methods due to the many obvious advantages. Firstly, by designing the tails of the primers one can easily d ...
A modified hyperplane clustering algorithm
A modified hyperplane clustering algorithm

... Partitioning methods such as k-means or SOM are more suitable to cluster the larger datasets. What factors can affect the speed and accuracy of clustering methods? ◦ The size and complexity of the data. ◦ How the data is processed before analysis. ...
Transcription and RNA processing
Transcription and RNA processing

... In bacterial cells, the holoenzyme (RNA polymerase plus sigma) recognizes and binds directly to sequences in the promoter. In eukaryotic cells, promoter recognition is carried out by accessory proteins (transcription factors) that bind to the promoter and then recruit a specific RNA polymerase (I, II ...
No Slide Title
No Slide Title

... random diffusion (that limits the constant to 108/M-1Sec-1). The measured rate constant for association with a 60 bp target is 1014/M-1Sec-1. If the target is the whole genome the rate constant is around 1014/M-1Sec-1. But how does the polymerase move from random binding sites to promoters? Perhaps ...
Membrane Adsorbers as a Tool for Rapid Purification
Membrane Adsorbers as a Tool for Rapid Purification

... used for purification, column contaminants, endotoxin, antibiotics, residual host cell proteins, DNA and viruses, where appropriate should be proved’. Pall Mustang membrane adsorbers made using quaternary ammonium, or ‘Q’, chemistry have a high affinity for negatively-charged molecules, such as DNA, ...
May 2003 - The Origin of Eukaryotic Cells, Part 2
May 2003 - The Origin of Eukaryotic Cells, Part 2

... propels other direction. If they have a group of flagella that surround the cell, they wrap around each other and rotate in unison, they move one way. To move in the other direction they unwrap, move to other end, wrap around each other, and rotate in unison. In other bacteria with multiple flagella ...
L14 Gene to Protein Fa08
L14 Gene to Protein Fa08

... – Joining of exons (expressed sequences) • Exons : coding regions of nucleotides – Exceptions – UTRs (untranslated regions) ...
Membrane Adsorbers as a Tool for Rapid
Membrane Adsorbers as a Tool for Rapid

... used for purification, column contaminants, endotoxin, antibiotics, residual host cell proteins, DNA and viruses, where appropriate should be proved’. Pall Mustang membrane adsorbers made using quaternary ammonium, or ‘Q’, chemistry have a high affinity for negatively-charged molecules, such as DNA, ...
How do proteins recognize DNA
How do proteins recognize DNA

... RNA polymerase traverses the template (antisense) strand, and following the rules of Watson-Crick complementarity with the antisense strand, creates an RNA copy of the sense (coding) strand. Polymerization is processive (without dissociation). Transcripts can be thousands or even millions of nucleot ...
Nucleic Acid Deoxyribose Nucleic Acid (DNA)Ribose Nucleic Acid
Nucleic Acid Deoxyribose Nucleic Acid (DNA)Ribose Nucleic Acid

... Gene is the functional unit of genome. Gene is a sequence of nucleic acid that produces another nucleic acid. Gene and Chromosome? DNA is organized into chromosomes which are found within the nuclei of cells. ...
Biosensors
Biosensors

The Evolution of Microbial Life: Prokaryotes, Protista - Jocha
The Evolution of Microbial Life: Prokaryotes, Protista - Jocha

... Genetic variability ultimately depends on… ...
Biosensors
Biosensors

... A device to determine a patient’s blood oxygen content: “the oximeter” The absorption spectra (α) of haemoglobin (Hb) and oxyhaemoglobin (HbO2) differ, which makes it possible to measure the ratio of both concentrations in blood by measuring the absorption of light of two different wavelengths, e.g. ...
2. Snuffle Snork
2. Snuffle Snork

... previously unknown creature. I have named this new life form Snorkus schmiddicus, or “Snork” for short. I was able to capture four different individuals and collect a sample of their DNA. They were then released unharmed, but pretty angry. Interestingly, the Snork species has only ten genes. Each ge ...
The Arabidopsis chloroplast ribosomal protein L21 is
The Arabidopsis chloroplast ribosomal protein L21 is

... RPL21c genes are constitutively expressed, but that only the RPL21c gene is highly expressed in leaves (Fig. 1). From these data, we conclude that two independent RPL21 genes exist in A. thaliana. Computer analysis of the Arabidopsis RPL21 genes using different algorithms (see Section 2) clearly pre ...
Isolation and characterization of Plasmid DNA from clinically
Isolation and characterization of Plasmid DNA from clinically

... once evolved, resistance genes can spread through the ...
Phenetics vs. Cladistics
Phenetics vs. Cladistics

... are characters of organisms that vary. The variation can be characterized by counting or measuring or describing it. ...
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