Genome & Protein “ Sequence Analysis Programs”
... AutoEditor : Automated correction of sequencing and basecaller errors a tool for correcting sequencing and basecaller errors using sequence alignment and chromatogram data. On average AutoEditor corrects 80% of erroneous base calls. It also greatly improves our ability to discover SNPs between close ...
... AutoEditor : Automated correction of sequencing and basecaller errors a tool for correcting sequencing and basecaller errors using sequence alignment and chromatogram data. On average AutoEditor corrects 80% of erroneous base calls. It also greatly improves our ability to discover SNPs between close ...
RNA
... to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the nucleus. ...
... to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the nucleus. ...
Restriction Enzyme Sequence
... Each enzyme recognizes a short, specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-stranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the ...
... Each enzyme recognizes a short, specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-stranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the ...
Nucleic Acids - Structure and Replication
... stained the sections and viewed them with an optical microscope. For each section, the student counted the number of cells in mitosis and the total number of cells in each field of view. She then calculated a mitotic index for each section using the equation: ...
... stained the sections and viewed them with an optical microscope. For each section, the student counted the number of cells in mitosis and the total number of cells in each field of view. She then calculated a mitotic index for each section using the equation: ...
Chapter 21: Genomics I: Analysis of DNA and Transposable Elements
... types are discussed. The section also introduces the Human Genome Project, which represents one of the most important undertakings in the study of genetics. Its goal, to map the human genome, was completed in 2006, but the analysis of the material may take decades. As students of genetics, you shoul ...
... types are discussed. The section also introduces the Human Genome Project, which represents one of the most important undertakings in the study of genetics. Its goal, to map the human genome, was completed in 2006, but the analysis of the material may take decades. As students of genetics, you shoul ...
Part 2
... 2. IPG strip: Commercially available immobilized pH gradient (IPG) gel strips have considerably facilitated the process of isoelectric focusing by eliminating the tedious steps of gel preparation and pH gradient establishment using ampholyte solutions. These strips, available across the pH range, co ...
... 2. IPG strip: Commercially available immobilized pH gradient (IPG) gel strips have considerably facilitated the process of isoelectric focusing by eliminating the tedious steps of gel preparation and pH gradient establishment using ampholyte solutions. These strips, available across the pH range, co ...
RNA PP
... to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the nucleus. ...
... to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the nucleus. ...
Unit 4
... Euchromatin- the more open unraveled form of eukaryotic chromatin, which is available for transcription. ...
... Euchromatin- the more open unraveled form of eukaryotic chromatin, which is available for transcription. ...
Molecular taxonomy,use of modern methods in the identification of a
... a certain molecular weight. The pattern usually varies slightly between gels. Since every organism will be giving a different RFLP patterns, its pattern of bands obtained corresponds to its DNA fingerprint. Because standard agarose gel electrophoresis fails to efficiently resolve fragments that are ...
... a certain molecular weight. The pattern usually varies slightly between gels. Since every organism will be giving a different RFLP patterns, its pattern of bands obtained corresponds to its DNA fingerprint. Because standard agarose gel electrophoresis fails to efficiently resolve fragments that are ...
New Lead Found in Serial Rapes: After Decades, DNA Links the
... for DNA because the statute of limitations had expired, meaning no charges could be filed if a suspect is caught. The state time limit to charge someone with rape was six years. "We may try to do it now," Lewis said of the DNA. "That's part of what we will discuss in the meeting." Recent changes in ...
... for DNA because the statute of limitations had expired, meaning no charges could be filed if a suspect is caught. The state time limit to charge someone with rape was six years. "We may try to do it now," Lewis said of the DNA. "That's part of what we will discuss in the meeting." Recent changes in ...
official course outline information
... CALENDAR DESCRIPTION: This is an intensive practical laboratory course that provides students with an applied introduction to the methodology used in recombinant DNA technology. The course encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysi ...
... CALENDAR DESCRIPTION: This is an intensive practical laboratory course that provides students with an applied introduction to the methodology used in recombinant DNA technology. The course encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysi ...
Chapter 4A
... Protein domains can be encoded by a single exon or by a small collection of exons within a larger gene. The coding regions for domains can be spliced in or out of the primary transcript by the process of alternative splicing. The resulting mRNAs encode different forms of the protein, known as isofor ...
... Protein domains can be encoded by a single exon or by a small collection of exons within a larger gene. The coding regions for domains can be spliced in or out of the primary transcript by the process of alternative splicing. The resulting mRNAs encode different forms of the protein, known as isofor ...
DNA Fingerprinting Name
... If you were on trial or were trying to identify an endangered species, would you want to rely on a technician’s eyeball estimate of a match, or would you want some more accurate measurement? In order to make the most accurate comparison between the crime scene DNA and the suspect DNA, other than jus ...
... If you were on trial or were trying to identify an endangered species, would you want to rely on a technician’s eyeball estimate of a match, or would you want some more accurate measurement? In order to make the most accurate comparison between the crime scene DNA and the suspect DNA, other than jus ...
Homework 1 / Introduction General questions Programming tasks
... name in the beginning as the author of the script. Make a clear distinction between the exercises and format the output in a clear and understandable way. If for some reasons some of the exercises are not completed, write to the output: "5. Task not completed due to ..." and you can also describe wh ...
... name in the beginning as the author of the script. Make a clear distinction between the exercises and format the output in a clear and understandable way. If for some reasons some of the exercises are not completed, write to the output: "5. Task not completed due to ..." and you can also describe wh ...
BIO 402/502 Advanced Cell & Developmental Biology
... DNA Hybridization • DNA hybridization is the process whereby complementary strand of DNA anneals (to form a double helix) with the single stranded DNA • Hybridization can be measured by labeling the “complementary strand” either with 32P nucleotides or fluorescent probes . • There is also DNA-RNA h ...
... DNA Hybridization • DNA hybridization is the process whereby complementary strand of DNA anneals (to form a double helix) with the single stranded DNA • Hybridization can be measured by labeling the “complementary strand” either with 32P nucleotides or fluorescent probes . • There is also DNA-RNA h ...
Protocol for MasterPure™ Gram Positive DNA
... The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse™ Lysozyme and the Gram Positive Cell Lysis Solution. Ready-Lyse Lysozyme is a stable solution of a non-ma ...
... The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse™ Lysozyme and the Gram Positive Cell Lysis Solution. Ready-Lyse Lysozyme is a stable solution of a non-ma ...
Forensic Dentistry
... This is accomplished by comparison of a victim’s dentition and supporting structures with dental records of known individuals. The latter records may be obtained from private dental offices, prison or military dental databases, or records retained by the Federal Bureau of Investigation through its N ...
... This is accomplished by comparison of a victim’s dentition and supporting structures with dental records of known individuals. The latter records may be obtained from private dental offices, prison or military dental databases, or records retained by the Federal Bureau of Investigation through its N ...
agarose gel - Bio
... vacuum filtration or column separation. The purified DNA fragments are of high quality and are ...
... vacuum filtration or column separation. The purified DNA fragments are of high quality and are ...
1.1 Biological Background
... a certain amino acid (see below). In eukaryotes, the mRNA is formed of coding and noncoding regions. Coding regions are the regions used to carry real genetic information. Non coding regions do not carry such information (see below). The coding regions are called exons, since they are able to leave ...
... a certain amino acid (see below). In eukaryotes, the mRNA is formed of coding and noncoding regions. Coding regions are the regions used to carry real genetic information. Non coding regions do not carry such information (see below). The coding regions are called exons, since they are able to leave ...
Phylogenetic DNA profiling : a tool for the investigation of poaching
... procedure. Reactions were performed in a total volume of 25 µl using 1-10 ng template DNA. The final concentration of the components in reaction mix was as follows; 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl2, 0.25 µM each primer, 200 µM dNTP’s, 1 unit Taq polymerase (Life Technologies, Austral ...
... procedure. Reactions were performed in a total volume of 25 µl using 1-10 ng template DNA. The final concentration of the components in reaction mix was as follows; 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl2, 0.25 µM each primer, 200 µM dNTP’s, 1 unit Taq polymerase (Life Technologies, Austral ...
212 Chapter 28 Biomolecules: Heterocycles and Nucleic Acids
... DNA replication occurs with very high fidelity: Most DNA polymerases have high intrinsic fidelity Many DNA polymerases have “proof-reading” (exonuclease) activity Mismatch repair proteins seek out and repair base-pair mismatches due to unfaithful replication 28.13 Structure and Synthesis of RNA: Tra ...
... DNA replication occurs with very high fidelity: Most DNA polymerases have high intrinsic fidelity Many DNA polymerases have “proof-reading” (exonuclease) activity Mismatch repair proteins seek out and repair base-pair mismatches due to unfaithful replication 28.13 Structure and Synthesis of RNA: Tra ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).