• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
lecture_ch05_2014 honors biology_website
lecture_ch05_2014 honors biology_website

... remain, however, about the safety of these foods given that their development relies on such new technology and about the long-term financial advantages they offer. ...
for Genetic Testing
for Genetic Testing

... • Satellites: the repeated unit typically ranges from 20 to 175 bp, and the length of the entire repeat is from 0.1 to 1 Mb. Satellite DNA is clustered in centromeric regions and is rarely used in genetic testing. • Minisatellites: the repeated unit typically ranges from 20 to 70 bp, and the length ...
Breeding - Farming Ahead
Breeding - Farming Ahead

... identify the likely parents of a lamb. It is known as fingerprinting technology because, like fingerprints, DNA does not change and it is possible to determine the identity of a person or animal from a tiny blood sample. The technology is also based on methods used to help resolve disputes of human ...
Protein Synthesis
Protein Synthesis

DNA - department of computer & electrical engineering and
DNA - department of computer & electrical engineering and

... A bacteria contains about 600,000 DNA base pairs Human and mouse genomes have some 3 billion. Each chromosome contains many genes. ...
DNA replication
DNA replication

... Alternative splicing • There are more than 1,000,000 different human antibodies. How is this possible with only ~30,000 genes? • Alternative splicing refers to the different ways of combining a gene’s exons. This can produce different forms of a protein for the same gene. • Alternative pre-mRNA spl ...
user instructions
user instructions

15 points each
15 points each

From DNA to Protein synthesis lab
From DNA to Protein synthesis lab

... construction must be packaged and transferred out of the DNA "library." First, the specific sequence of DNA that codes for the protein is transcribed into a complementary strand of mRNA. In eukaryotic cells, the mRNA then leaves the nucleus and enters the cl.toplasm. In all cells, the mRNA molecule ...
Document
Document

... percentage of nucleotides contain the pyrimidine base, thymine (T)? ___23%____________ 18. How many bases are there in 30 kb of DNA? ___________60,000________________________ 19. What anticodon sequence occurs in tRNA species carrying tryptophan? __5´CCA3´_____ 20. The most common elements in living ...
Chromosome, genes and DNA Task 1 chromos
Chromosome, genes and DNA Task 1 chromos

IB Topics DNA HL no writing
IB Topics DNA HL no writing

... • RNA polymerase controls transcription / is the enzyme used in transcription; • DNA is unwound by RNA polymerase; • DNA is split into two strands; • mRNA is made by transcription; • promoter region (by start of gene) causes RNA polymerase to bind; • anti-sense / template strand of DNA is transcrib ...
slides for SMART-FHIR_rr_ga
slides for SMART-FHIR_rr_ga

... ● file : attachment // 0..* File generated from the lab for analysis ...
Biotechnology
Biotechnology

... • EcoRI binds & cuts DNA at the following sequence: – 5 ... GAATTC ... 3 – 3... CTTAAG ... 5 • The sequence is palindromic: – reads the same 5-to-3 on both strands. ...
Gene Technology Powerpoint
Gene Technology Powerpoint

... DNA profiling uses repetitive ("repeat") sequences that are highly variable,[2] called variable number tandem repeats (VNTRs), VNTR locations on a gene (loci) are very similar between closely related humans, but so variable that unrelated individuals are extremely unlikely to have the same VNTRs. ...
T - 서울대 : Biointelligence lab
T - 서울대 : Biointelligence lab

...  [Guarneiri et al., 1996]  Non-procedural ...
PowerPoint 簡報
PowerPoint 簡報

HY asiakirjapohja - Hercules Project
HY asiakirjapohja - Hercules Project

... - Days from receiving the samples to delivering the data in Phase I and Phase II separately. RNA, WGS and methylation data may have different throughput times. - General sample requirements. - Prices separately for DNA 1) sample and library quality control and preparation and 2) for sequencing. This ...
2017 - Barley World
2017 - Barley World

... a. failure of transcription of the gene to a mRNA. b. failure to add a 3’ tail to the mRNA corresponding to this gene. c. failure of translation of the mRNA corresponding to this gene. d. failure of the protein to assume its three-dimensional configuration. 15. The HvCBF2 gene used as an example in ...
Population Genetics and a Study of Speciation Using Next
Population Genetics and a Study of Speciation Using Next

... to assemble the accessory gland transcriptome of crickets de novo (from scratch). They generated enough of these sequencing reads to cover each site in the transcriptome an average of four times (4· coverage). Sequence assembly was accomplished by finding fragments of sequence that shared identical b ...
plotfold
plotfold

... ----------------------------------------------Product Melting Temperature (degrees Celsius) Minimum (range 0.0 thru 200.0) Maximum ...
- fiveless|notes
- fiveless|notes

... By carrying out Southern blotting, we can detect the presence of disease allele through the characteristic banding pattern that results on the electrophoresis gel. In flanking region In disease phenylketonuria (PKU), RFLP in question results from change in DNA sequence flanking 3’ region. By carryin ...
Faik Bioinformatics PowerPoint 1-2006
Faik Bioinformatics PowerPoint 1-2006

DNA and Genetic Material
DNA and Genetic Material

... this process • A-T base pairs have two hydrogen bonds strands rich in these nucleotides are generally easier to separate due the positive relationship between the number of hydrogen bonds and the difficulty of breaking these bonds. ...
Recombinant DNA Technology
Recombinant DNA Technology

... – Needs template DNA and two primers that flank the region to be amplified. Primers are short (generally 18-30 bases) DNA oligonucleotides complementary to the ends of the region being amplified. – DNA polymerase adds new bases to the 3' ends of the primers to create the new second strand. – go from ...
< 1 ... 171 172 173 174 175 176 177 178 179 ... 353 >

Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report