Additional file 5: Method SI Constructions of other knock
... segment carrying the 1.4-kb aac(3)IV cassette were ligated into EcoRI-digested pIJ2925[1], a pUC18 derivative with a multi-cloning site flanked by BglII, to generate pJTU762. A BglII segment from pJTU762 was ligated into BamHI-digested pHZ1358[2], an E. coli-Streptomyces shuttle vector, to generate ...
... segment carrying the 1.4-kb aac(3)IV cassette were ligated into EcoRI-digested pIJ2925[1], a pUC18 derivative with a multi-cloning site flanked by BglII, to generate pJTU762. A BglII segment from pJTU762 was ligated into BamHI-digested pHZ1358[2], an E. coli-Streptomyces shuttle vector, to generate ...
DNA Replication
... fMet-tRNA complex will be bound, is distinguished from other similar codons by its proximity to the Shine-Dalgarno sequence. ...
... fMet-tRNA complex will be bound, is distinguished from other similar codons by its proximity to the Shine-Dalgarno sequence. ...
Simulating Protein Synthesis 01/04
... and thus the structure and function of proteins. In a process called transcription, which takes place in the nucleus of the cell, RNA polymerase reads and copies the DNA's nucleotide sequence, creating a complementary messenger RNA (mRNA) molecule. Then the mRNA carries this information in the form ...
... and thus the structure and function of proteins. In a process called transcription, which takes place in the nucleus of the cell, RNA polymerase reads and copies the DNA's nucleotide sequence, creating a complementary messenger RNA (mRNA) molecule. Then the mRNA carries this information in the form ...
PDF
... An in vitro cleavage assay for genotype determination The Guideit Genotype Confirmation Kit uses a Cas9/sgRNAmediated in vitro cleavage assay for genotype determination. PCR amplicons containing the potentially edited genomic locus are used in a cleavage reaction set up with the same sgRNA as was ...
... An in vitro cleavage assay for genotype determination The Guideit Genotype Confirmation Kit uses a Cas9/sgRNAmediated in vitro cleavage assay for genotype determination. PCR amplicons containing the potentially edited genomic locus are used in a cleavage reaction set up with the same sgRNA as was ...
learning outcomes - McGraw Hill Higher Education
... also has been used; PCR biases exist that confound results to some extent 3. Hierarchical oligonucleotide primer extension (HOPE) allows the detection of multiple SSU rRNA genes in a single sample; different primers (multiplex PCR) having poly-A extensions of different lengths allow for discriminati ...
... also has been used; PCR biases exist that confound results to some extent 3. Hierarchical oligonucleotide primer extension (HOPE) allows the detection of multiple SSU rRNA genes in a single sample; different primers (multiplex PCR) having poly-A extensions of different lengths allow for discriminati ...
Epigenetic and genetic factors affect transgene
... mouse embryo. Cold Spring Harbor Laboratory. LAIRD, C. D. (1987). Proposed mechanism of inheritance and expression of the human fragile-X syndrome of mental ...
... mouse embryo. Cold Spring Harbor Laboratory. LAIRD, C. D. (1987). Proposed mechanism of inheritance and expression of the human fragile-X syndrome of mental ...
zdar_report - Princeton University
... experiment. I used two medium length n-alkanes: Octane (C-8) and Dodecane (C-12), one long length n-alkane: Hexadecane (C-16). The fourth enrichment culture was glucose, which was used as a positive control, as all bacteria should be able to utilize glucose. After two weeks, DNA was extracted from t ...
... experiment. I used two medium length n-alkanes: Octane (C-8) and Dodecane (C-12), one long length n-alkane: Hexadecane (C-16). The fourth enrichment culture was glucose, which was used as a positive control, as all bacteria should be able to utilize glucose. After two weeks, DNA was extracted from t ...
AP Biology Fall Semester Review
... three components. Which of these components could be removed from a nucleotide that is part of a nucleic acid chain without breaking the chain? a. sugar b. phosphate c. nitrogenous base 30) The structure of DNA as proposed by Crick and Watson depended on all of the following ...
... three components. Which of these components could be removed from a nucleotide that is part of a nucleic acid chain without breaking the chain? a. sugar b. phosphate c. nitrogenous base 30) The structure of DNA as proposed by Crick and Watson depended on all of the following ...
DNA History - Biology Junction
... DNA composition: “Chargaff’s rules” varies from species to species all 4 bases not in equal quantity bases present in characteristic ratio ...
... DNA composition: “Chargaff’s rules” varies from species to species all 4 bases not in equal quantity bases present in characteristic ratio ...
secret codon
... sequence of three DNA bases, called a codon. Since it takes three DNA bases to designate an amino acid, there are enough combinations of the 4 different bases to represent all of the amino acids, as well as three stop codons that indicate when the protein ends. Each base can be in any position, whic ...
... sequence of three DNA bases, called a codon. Since it takes three DNA bases to designate an amino acid, there are enough combinations of the 4 different bases to represent all of the amino acids, as well as three stop codons that indicate when the protein ends. Each base can be in any position, whic ...
Personal Genetics: PCR Determination of PTC Tasters
... a “taster” allele that encodes a protein that can detect the bitter PTC chemical and a “non-taster” allele that encodes a faulty protein that does not detect the bitter PTC chemical. Researchers have identified 2 DNA changes between the taster and non-taster alleles. These differences between the ta ...
... a “taster” allele that encodes a protein that can detect the bitter PTC chemical and a “non-taster” allele that encodes a faulty protein that does not detect the bitter PTC chemical. Researchers have identified 2 DNA changes between the taster and non-taster alleles. These differences between the ta ...
Background Information
... DNA molecules in precise positions. Restriction endonucleases are important for recombinant DNA technology because they cut DNA at specific sites. These enzymes are usually made by bacterial species in which they degrade invading foreign DNA within the bacterial cell. Most restriction enzymes recogn ...
... DNA molecules in precise positions. Restriction endonucleases are important for recombinant DNA technology because they cut DNA at specific sites. These enzymes are usually made by bacterial species in which they degrade invading foreign DNA within the bacterial cell. Most restriction enzymes recogn ...
Ans8. Anaerobic Respiration/ Fermentation
... forward in both discriminating power and the ability to recover information from very small (or degraded) starting samples. PCR greatly amplifies the amounts of a specific region of DNA, using oligonucleotide primers and a thermostable DNA polymerase. The PCR method is readily adaptable for analyzin ...
... forward in both discriminating power and the ability to recover information from very small (or degraded) starting samples. PCR greatly amplifies the amounts of a specific region of DNA, using oligonucleotide primers and a thermostable DNA polymerase. The PCR method is readily adaptable for analyzin ...
Taster Lab Student Doc PDF
... a “taster” allele that encodes a protein that can detect the bitter PTC chemical and a “non-taster” allele that encodes a faulty protein that does not detect the bitter PTC chemical. Researchers have identified 2 DNA changes between the taster and non-taster alleles. These differences between the ta ...
... a “taster” allele that encodes a protein that can detect the bitter PTC chemical and a “non-taster” allele that encodes a faulty protein that does not detect the bitter PTC chemical. Researchers have identified 2 DNA changes between the taster and non-taster alleles. These differences between the ta ...
Sterically stabilized self-assembling reversibly cross
... to compare the performance of the small DNA tracers with the existing Rhodamine WT dye used in water-tracing applications. Some details of the River Fruin trial are given here. The River Fruin is well channelled, and the trial took place over a 6.1 km stretch of the river. After depositing the dye [ ...
... to compare the performance of the small DNA tracers with the existing Rhodamine WT dye used in water-tracing applications. Some details of the River Fruin trial are given here. The River Fruin is well channelled, and the trial took place over a 6.1 km stretch of the river. After depositing the dye [ ...
5X All-In-One RT MasterMix
... necessary for first-strand cDNA synthesis with the exception of the template. This optimized, 5X concentrated reaction MasterMix contains abm’s proprietary OneScript® Reverse Transcriptase (OneScript® RTase), RNaseOFF Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo(dT)s and Random ...
... necessary for first-strand cDNA synthesis with the exception of the template. This optimized, 5X concentrated reaction MasterMix contains abm’s proprietary OneScript® Reverse Transcriptase (OneScript® RTase), RNaseOFF Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo(dT)s and Random ...
Genetic Technology
... Sequencing the human genome • The difficult job of sequencing the human genome is begun by cleaving samples of DNA into fragments using restriction enzymes. • Then, each individual fragment is cloned and sequenced. The cloned fragments are aligned in the proper order by overlapping matching sequenc ...
... Sequencing the human genome • The difficult job of sequencing the human genome is begun by cleaving samples of DNA into fragments using restriction enzymes. • Then, each individual fragment is cloned and sequenced. The cloned fragments are aligned in the proper order by overlapping matching sequenc ...
Detecting Gene Polymorphisms- PCR
... that requires induction of expression and so is slow to respond to occasional alcohol consumption. Another is a pair of enzymes, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), that catalyze a stepwise oxidation of ethanol to acetylaldehyde to acetic acid. There are families of ADH an ...
... that requires induction of expression and so is slow to respond to occasional alcohol consumption. Another is a pair of enzymes, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), that catalyze a stepwise oxidation of ethanol to acetylaldehyde to acetic acid. There are families of ADH an ...
Gibson Assembly™ – Building a Synthetic Biology Toolset
... Cloning. Gibson Assembly eliminates the need to engineer restriction enzyme cut sites within DNA when assembling fragments together. DNA molecules are designed such that neighboring fragments contain a 20-40 bp overlapping sequence. If the DNA fragments originate from PCR products, the overlapping s ...
... Cloning. Gibson Assembly eliminates the need to engineer restriction enzyme cut sites within DNA when assembling fragments together. DNA molecules are designed such that neighboring fragments contain a 20-40 bp overlapping sequence. If the DNA fragments originate from PCR products, the overlapping s ...
NAME: ________________ DATE: ____________ BLOCK: _____
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
pGLO2011 Wilkes
... The phenomenon of transformation, which provided a key clue to understanding the molecular basis of the gene, also provided a tool for manipulating the genetic makeup of living organisms. To a large extent, genetic engineering relies on adding relatively short segments of DNA containing a foreign o ...
... The phenomenon of transformation, which provided a key clue to understanding the molecular basis of the gene, also provided a tool for manipulating the genetic makeup of living organisms. To a large extent, genetic engineering relies on adding relatively short segments of DNA containing a foreign o ...
Chapter 17 – Molecular genetics
... •The two original strands of DNA are shown in yellow (light); newly synthesized DNA is blue (dark) • Conservative replication would leave intact the original DNA molecule and generate a completely new molecule. • Dispersive replication would produce two DNA molecules with sections of both old and ne ...
... •The two original strands of DNA are shown in yellow (light); newly synthesized DNA is blue (dark) • Conservative replication would leave intact the original DNA molecule and generate a completely new molecule. • Dispersive replication would produce two DNA molecules with sections of both old and ne ...
ES5
... Sequencing and “Barcode Analysis” of Microbial Communities PCR products from Bacteria and Fungal reactions are in cue to be sequenced on an Illumina MiSEQ platform. The results from this technology are analogous to data derived from cloning and sequencing in that single-stranded reads are generated ...
... Sequencing and “Barcode Analysis” of Microbial Communities PCR products from Bacteria and Fungal reactions are in cue to be sequenced on an Illumina MiSEQ platform. The results from this technology are analogous to data derived from cloning and sequencing in that single-stranded reads are generated ...
Ho - Engineering Computing Facility
... temperatures used. Because the error rate is on a per base duplication basis, the fraction of mutant tags is then dependent on the number of bases within each ditag, N. Errors that are created in early PCR cycles are also propagated in subsequent cycles, so replication of existing false ditags and i ...
... temperatures used. Because the error rate is on a per base duplication basis, the fraction of mutant tags is then dependent on the number of bases within each ditag, N. Errors that are created in early PCR cycles are also propagated in subsequent cycles, so replication of existing false ditags and i ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).