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... EXAMPLE: Halanaerobium salsugo ...
Biotechnology and Genetic Engineering
Biotechnology and Genetic Engineering

... screen a cDNA library •Using a DNA probe with a homologous sequence (e.g., a homologous cDNA or gene clone from a related species) •Using an oligonucleotide probe based on a known amino acid sequence (requires purification of the protein and some peptide sequencing) •Using an antibody against the pr ...
Southern Blotting
Southern Blotting

... • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA. • Moreover, RFLP is a sequence of DNA that has a restriction site on each end with a "target" sequence in between. A target sequence is any segment of ...
Title: GeneWiz browser: An Interactive Tool for Visualizing
Title: GeneWiz browser: An Interactive Tool for Visualizing

... (green-to-blue lane) plotted along with reference genome. • This figure shows that a good correspondence between the in-silico and experimental reads suggests little bias towards certain chromosomal regions if read coverage is around 40 ...
Gene Q
Gene Q

... PCR primers were used to amplify Sperm number DNA isolated from one man's ...
Conference title
Conference title

... full length transcripts Successfully predicts many isoforms as well Grabherr et al. 2011 ...
Document
Document

... •Avery, McCarty and MacLeod (1944) proved that DNA is the transforming principle . ...
Certificate of Analysis MicroSeq(R) 500 16S rDNA
Certificate of Analysis MicroSeq(R) 500 16S rDNA

Annelise Mah - New Genomics Technology: Copy Number Variation Analysis Methods
Annelise Mah - New Genomics Technology: Copy Number Variation Analysis Methods

... elements of PCR are fluorescently tagged and the intensity of the glow is monitored between cycles. Comparatively, a single gene with more copies will increase sooner than one with fewer copies when the fluorescent glow is measured on a logarithmic scale. A more dilute sample will increase later (gr ...


... chromosome of >20 Mb interstitially or >10 Mb telomerically (15 and 8 Mb, respectively, for imprinted chromosomes). * Contiguous homozygosity of >8 Mb within multiple chromosomes suggests common descent. These regions of potential recessive allele risk are designated. * A high level of allele homozy ...
Illumina Solexa
Illumina Solexa

... four separate fluorophores are all present and followed over time. Since it is the native polymerase (albeit modified), very long reads can be obtained but since it is a single molecule, error rates are ...
C. Nucleic acid hybridization assays using cloned target DNA, and
C. Nucleic acid hybridization assays using cloned target DNA, and

... gene specific oligonucleotide microarrays • Screening of DNA variation in disease genes as in breast cancer susceptibility gene BRCA1. Also identifying and cataloging human single nucleotide polymorphism (SNP) markers. ...
S1230Datasheet-Lot0031301
S1230Datasheet-Lot0031301

What is a southern blot?
What is a southern blot?

...  They used 2 steps for determining the β-globin genotype of human genomic DNA samples: 1. From β-globin gene sequence spanning the polymorphic Dde I restriction site diagnostic of the βa allele is amplified 2. The presence of Dde I restriction site in the amplified DNA simple is determined by solut ...
Document
Document

... a) All of these enzymes leave ends that are compatible with ends generated by the others; b) None of the enzymes produce compatible ends; c) Only BamHI and BglII fragments are compatible; d) Only BamHI and SauIIIa fragments are compatible; e) only BglII and SauIIIa fragments are compatible. 3. True ...
direct genetic testing
direct genetic testing

... How does forensic ID work? Extract DNA Analyse specific regions using probes look for matches between 2 samples at ...
命題標頭紙 - 慈濟大學醫學資訊學系所
命題標頭紙 - 慈濟大學醫學資訊學系所

... 9. A BLAST search yields several hits. Among them, subject A with 95% identity and E-value = 0.1, and subject B with 65% identity and E-value = e-32. Which one is a better hit? Why? (5%) 10. Palindromes are DNA sequences in which the reverse complement is identical to the positive strand, such as GT ...
Genetics Objectives 15
Genetics Objectives 15

... genes on the same chromosome can be separated during meiosis. The closer the genes are to each other, the less likely that a crossing over event will occur between them, and the more closely linked they are. Morgan (108 base pairs): the unit of length for one crossing over to happen every time Centi ...
Midterm 1 Results…
Midterm 1 Results…

... ~ 1 SNP per 1000 bp => 3 million  Stable genetic markers: mutation rate ~ 2 x 10-8/site/gen How many new SNPs do you carry? You’re a ...
Ch 20 GR
Ch 20 GR

... 37. Define single nucleotide polymorphisms. 38. What are some examples of the medical applications of biotechnology? ...
Electrochemical DNA Biosensors
Electrochemical DNA Biosensors

... • significantly enhanced selectivity can be achieved by the use of peptide nucleic acid (PNA) probes. ...
Warheit#2
Warheit#2

... with acoustic transmitters, and we defined survival as a fish’s detection at Strait of Juan de Fuca (JDF) acoustic detection array. We genotyped the fish using restriction-site associated DNA (RAD) sequences (RAD-seq). RAD-seq is a genome complexity reduction technique that generally produces 1000s ...
Chapter 21: Molecular Basis of Cancer
Chapter 21: Molecular Basis of Cancer

... genotyping •MIP genotyping uses circularizable probes with 5′ and 3′ ends that anneal upstream and downstream of the ...
Experimental Ecology
Experimental Ecology

... Principals of genotypic detection methods • Methods are based on the fact that nucleic acids are made up of 4 bases arranged in a specific order • Base sequences are conserved from one generation to the next • DNA molecules are double-stranded ...
What are the methods and approaches used to identify and
What are the methods and approaches used to identify and

... Isolation of DNA From Plant ...
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Molecular Inversion Probe



Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.
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