Tuesday 4/8/14
... • 1- Extract DNA from cell nucleus • 2- Add restriction enzymes to cut DNA into pieces • 3- Separate fragments with gel electrophoresis • 4- Make a copy of results using southern blot • 5- add radioactive DNA probe to visualize • 6- visualize fragments and analyze results ...
... • 1- Extract DNA from cell nucleus • 2- Add restriction enzymes to cut DNA into pieces • 3- Separate fragments with gel electrophoresis • 4- Make a copy of results using southern blot • 5- add radioactive DNA probe to visualize • 6- visualize fragments and analyze results ...
Repair of Site-Specific DNA Double-Strand Breaks in
... DSB repair by NHEJ is usually accompanied by loss or gain (or loss and gain) of nucleotides. Therefore, we evaluated the efficiency of DSB repair via NHEJ by testing for short deletions (<30 bp; often linked with classical NHEJ) and longer deletions (indicating alternative end joining; Deriano and Ro ...
... DSB repair by NHEJ is usually accompanied by loss or gain (or loss and gain) of nucleotides. Therefore, we evaluated the efficiency of DSB repair via NHEJ by testing for short deletions (<30 bp; often linked with classical NHEJ) and longer deletions (indicating alternative end joining; Deriano and Ro ...
Ch 23 Evolution - philipdarrenjones.com
... Muta*ons That Alter Gene Number or Sequence • Chromosomal muta4ons that delete, disrupt, or rearrange many loci are typically harmful • Duplica4on • Usually harmful • Small pieces some4mes less harmful ...
... Muta*ons That Alter Gene Number or Sequence • Chromosomal muta4ons that delete, disrupt, or rearrange many loci are typically harmful • Duplica4on • Usually harmful • Small pieces some4mes less harmful ...
Genome Evolution, Chromosomal Mutations, Paralogy
... One Cell, One Genome, One Replication Every cell holds a copy of all its DNA = its genome. The human body is made of ~1013 cells. All originate from a single cell through repeated cell divisions. DNA strings = Chromosomes ...
... One Cell, One Genome, One Replication Every cell holds a copy of all its DNA = its genome. The human body is made of ~1013 cells. All originate from a single cell through repeated cell divisions. DNA strings = Chromosomes ...
Recombinant DNA Technology
... inefficient, so bacteria that aren’t transformed must be killed. Most cloning vectors have a multiple cloning site, a short region of DNA containing many restriction sites close together (also called a polylinker). This allows many different restriction enzymes to be used. Most cloning vectors use a ...
... inefficient, so bacteria that aren’t transformed must be killed. Most cloning vectors have a multiple cloning site, a short region of DNA containing many restriction sites close together (also called a polylinker). This allows many different restriction enzymes to be used. Most cloning vectors use a ...
Microarray Analysis 1
... DNA microarray is a new technology to measure the level of the mRNA gene products of a living cell. A microarray chip is a rectangular chip on which is imposed a grid of DNA spots. These spots form a two dimensional array. Each spot in the array contains millions of copies of some DNA strand, bonded ...
... DNA microarray is a new technology to measure the level of the mRNA gene products of a living cell. A microarray chip is a rectangular chip on which is imposed a grid of DNA spots. These spots form a two dimensional array. Each spot in the array contains millions of copies of some DNA strand, bonded ...
this PDF file - Undergraduate Science Journals
... Pseudomonas aeroginosa (PA) is a gram negative aerobic rod that can cause serious, possibly life threatening, disease in humans, plants, and animals. In order to develop new and better treatments against PA, it is important to understand how this bacterium works to survive. Azoreductase from PA has ...
... Pseudomonas aeroginosa (PA) is a gram negative aerobic rod that can cause serious, possibly life threatening, disease in humans, plants, and animals. In order to develop new and better treatments against PA, it is important to understand how this bacterium works to survive. Azoreductase from PA has ...
fingerprint - West Essex Regional School District
... specific places, it will form different sized DNA fragments ◦ Using a technology called Gel Electrophoresis, these DNA fragments can be separated within a gel ◦ The results will form band patterns (or fingerprint) in the gel specific to that individual ◦ Relatives may share some bands and this is ho ...
... specific places, it will form different sized DNA fragments ◦ Using a technology called Gel Electrophoresis, these DNA fragments can be separated within a gel ◦ The results will form band patterns (or fingerprint) in the gel specific to that individual ◦ Relatives may share some bands and this is ho ...
Chapter 7 Notes: DNA Profiling
... Reliability • Two major factors affecting reliability: population genetics and genetic statistics • Humans have thousands of RFLP loci or DNA segments that can be selected and used for fingerprinting analysis Can depend on demographic factors such as ethnicity or geographic isolation Humans only dif ...
... Reliability • Two major factors affecting reliability: population genetics and genetic statistics • Humans have thousands of RFLP loci or DNA segments that can be selected and used for fingerprinting analysis Can depend on demographic factors such as ethnicity or geographic isolation Humans only dif ...
Isolation of plasmid DNA
... Luria broth (LB): For 1 l: 10 g tryptone, 5 g yeast extract, 10 g NaCl (RMM 58.44), make up in double distilled and deionised water and pH to 7.0. Autoclave for use. [Small volumes may be filter sterilised]. Ampicillin, 10mg/ml. Filter sterilise [do not autoclave]. Store at –20oC for up to 1 year. U ...
... Luria broth (LB): For 1 l: 10 g tryptone, 5 g yeast extract, 10 g NaCl (RMM 58.44), make up in double distilled and deionised water and pH to 7.0. Autoclave for use. [Small volumes may be filter sterilised]. Ampicillin, 10mg/ml. Filter sterilise [do not autoclave]. Store at –20oC for up to 1 year. U ...
Full-Text PDF
... perturbations (mutations) are introduced into a particular gene of interest, and its impact is investigated through functional analysis [3]. Concomitant with the rapid accumulation of available genetic information, this reverse genetics approach is increasingly used for strain improvement in this sy ...
... perturbations (mutations) are introduced into a particular gene of interest, and its impact is investigated through functional analysis [3]. Concomitant with the rapid accumulation of available genetic information, this reverse genetics approach is increasingly used for strain improvement in this sy ...
emboj7601986-sup
... which the 2.2 Kb ClaI/EcoRV fragment (Figure S1A) was deleted and replaced with the neomycin phosphotransferase (neo) gene, under the control of the phosphoglycerate kinase promoter (pgk). Homologous recombination at the Crif1 locus resulted in the deletion of exon 1 (Figure S1A). Three E14K ES cell ...
... which the 2.2 Kb ClaI/EcoRV fragment (Figure S1A) was deleted and replaced with the neomycin phosphotransferase (neo) gene, under the control of the phosphoglycerate kinase promoter (pgk). Homologous recombination at the Crif1 locus resulted in the deletion of exon 1 (Figure S1A). Three E14K ES cell ...
View as PDF
... Genome Editing: Tools for CRISPR/Cas9 Applications Genome editing is enabled by the development of tools to make precise, targeted changes to the genome of living cells. Recent approaches to targeted genome modification – zinc-finger nucleases (ZFNs) and transcription-activator like effector nuclea ...
... Genome Editing: Tools for CRISPR/Cas9 Applications Genome editing is enabled by the development of tools to make precise, targeted changes to the genome of living cells. Recent approaches to targeted genome modification – zinc-finger nucleases (ZFNs) and transcription-activator like effector nuclea ...
doc
... Luria broth (LB): For 1 l: 10 g tryptone, 5 g yeast extract, 10 g NaCl (RMM 58.44), make up in double distilled and deionised water and pH to 7.0. Autoclave for use. [Small volumes may be filter sterilised]. Ampicillin, 10mg/ml. Filter sterilise [do not autoclave]. Store at –20oC for up to 1 year. U ...
... Luria broth (LB): For 1 l: 10 g tryptone, 5 g yeast extract, 10 g NaCl (RMM 58.44), make up in double distilled and deionised water and pH to 7.0. Autoclave for use. [Small volumes may be filter sterilised]. Ampicillin, 10mg/ml. Filter sterilise [do not autoclave]. Store at –20oC for up to 1 year. U ...
Recombinant Paper Plasmids Cut-and
... sequence of the gene. There are various other methods of finding genes. After scientists have identified the gene, they must isolate it. Restriction enzymes, or endonucleases, from bacterial cells are key in this step. They are isolated from bacteria that use them to destroy, by cleaving or cutting ...
... sequence of the gene. There are various other methods of finding genes. After scientists have identified the gene, they must isolate it. Restriction enzymes, or endonucleases, from bacterial cells are key in this step. They are isolated from bacteria that use them to destroy, by cleaving or cutting ...
Amgen Bruce Wallace Transformation Labs (2-7)
... Take a colony that has the gene of interest from the plate, put into broth to replicate, now we have tons of bacteria (so tons of our geneprotein!) Lab 7—Purification of mFP from an Overnight Culture ...
... Take a colony that has the gene of interest from the plate, put into broth to replicate, now we have tons of bacteria (so tons of our geneprotein!) Lab 7—Purification of mFP from an Overnight Culture ...
Gene mapping today: applications to farm animals
... To further improve the spatial resolution of gene mapping, co-hybridization studies with closely spaced probes from a single genomic region have been performed on interphase nuclei. Measurements of the distances between multiple pair probes, after hybridization to methanol-acetic acid-fixed flat nuc ...
... To further improve the spatial resolution of gene mapping, co-hybridization studies with closely spaced probes from a single genomic region have been performed on interphase nuclei. Measurements of the distances between multiple pair probes, after hybridization to methanol-acetic acid-fixed flat nuc ...
Reading frame
... In practice it is treated as a synonym for "computational molecular biology“ ----the use of computers to characterize the molecular components of living things. ...
... In practice it is treated as a synonym for "computational molecular biology“ ----the use of computers to characterize the molecular components of living things. ...
Allele replacement: an application that permits rapid manipulation of
... on media supplemented with 5% sucrose). Figure 3 illustrates and describes the mutagenesis procedure. Briefly, the in vitro altered sequences are transformed into E. coli and co-integrates (that arise as a result of homologous recombination), are selected by plating cells on cm/zeocin plates at the ...
... on media supplemented with 5% sucrose). Figure 3 illustrates and describes the mutagenesis procedure. Briefly, the in vitro altered sequences are transformed into E. coli and co-integrates (that arise as a result of homologous recombination), are selected by plating cells on cm/zeocin plates at the ...
Optimized DNA microarray assay allows detection and genotyping
... plasmid DNA by both microarray and real-time PCR (Table 1) indicates that the actual number of target copies available for amplification was lower than the number of genome copies present in the sample. This may be a consequence of shear stress in the course of DNA extraction, which can lead to stra ...
... plasmid DNA by both microarray and real-time PCR (Table 1) indicates that the actual number of target copies available for amplification was lower than the number of genome copies present in the sample. This may be a consequence of shear stress in the course of DNA extraction, which can lead to stra ...
Sequence Analysis of the y-Globin Gene Locus from
... (HPFH) is a benign condition in which fetal hemoglobin expression persists into adulthood at levels greater than 1% in the absence of erythropietic stress or thala~semia.’-~ Because this condition can be viewed as a failure to switch from fetal (HbF, a2y2)to adult (HbA, ad2)hemoglobin synthesis, it ...
... (HPFH) is a benign condition in which fetal hemoglobin expression persists into adulthood at levels greater than 1% in the absence of erythropietic stress or thala~semia.’-~ Because this condition can be viewed as a failure to switch from fetal (HbF, a2y2)to adult (HbA, ad2)hemoglobin synthesis, it ...
Unit 5: DNA
... organism that we will be using to see this concept in action. Question: How do proteins effect appearance? Background Knowledge: a) The process of making a protein from a gene is called ______________________________________________________________ and has two basic steps: __________________________ ...
... organism that we will be using to see this concept in action. Question: How do proteins effect appearance? Background Knowledge: a) The process of making a protein from a gene is called ______________________________________________________________ and has two basic steps: __________________________ ...
Annotation
... you’re ready to start calling genes. Stops are stops, so you can rest assured that when you run into an asterisk, you are at the end of that gene. (This is assuming that no nonsense suppressor or tRNAs are present.) However, picking gene start codons can be tricky. Gene startscalls are based on inpu ...
... you’re ready to start calling genes. Stops are stops, so you can rest assured that when you run into an asterisk, you are at the end of that gene. (This is assuming that no nonsense suppressor or tRNAs are present.) However, picking gene start codons can be tricky. Gene startscalls are based on inpu ...
Demonstration that the Neurospora crassa mutation un
... Schmidhauser et al. (1999) reported that un-4 was on a cosmid with lys-5, we attempted to complement the un-4 lesion using cosmid G13:G8 from the Orbach Sachs pMOcosX library (Orbach and Sachs, 1991; Vollmer and Yanofsky, 1986). While this cosmid was reported to complement un-4 (Schmidhauser et al., ...
... Schmidhauser et al. (1999) reported that un-4 was on a cosmid with lys-5, we attempted to complement the un-4 lesion using cosmid G13:G8 from the Orbach Sachs pMOcosX library (Orbach and Sachs, 1991; Vollmer and Yanofsky, 1986). While this cosmid was reported to complement un-4 (Schmidhauser et al., ...
Identity of zinc finger nucleases with specificity novel HSV-2 vaccine/therapy precursors
... description of the spacer regions was maintained at 5-7 base-pairs. These ZFNs can bind as dimers to their target HSV-2 DNA sites, with each monomer using its zinc finger domain to recognize a ‘half-site’ of the targeted DNA sequence. In-vivo, dimerization of ZFNs is mediated by the FokI cleavage do ...
... description of the spacer regions was maintained at 5-7 base-pairs. These ZFNs can bind as dimers to their target HSV-2 DNA sites, with each monomer using its zinc finger domain to recognize a ‘half-site’ of the targeted DNA sequence. In-vivo, dimerization of ZFNs is mediated by the FokI cleavage do ...