Exam 1 Key
... b) Circle the purines in your primer. c) If DNA polymerase is added to the template strand and primer given in part a), indicate the direction(s) of DNA synthesis with an arrow. Question 2. (15 points) The plant, Banana examinus, is diploid and 2n = 4. There is one long pair and one short pair of ch ...
... b) Circle the purines in your primer. c) If DNA polymerase is added to the template strand and primer given in part a), indicate the direction(s) of DNA synthesis with an arrow. Question 2. (15 points) The plant, Banana examinus, is diploid and 2n = 4. There is one long pair and one short pair of ch ...
ANTIVIRAL/ANTIFUNGAL AGENTS
... 500 mg TID x 10 days is comparable to high dose of acyclovir in treating zoster in immunocompromised patients & in opthalmic zoster Associated with dose-related reductions in Hepatitis B Virus DNA and transaminase levels in patients with chronic HBV hepatitis ...
... 500 mg TID x 10 days is comparable to high dose of acyclovir in treating zoster in immunocompromised patients & in opthalmic zoster Associated with dose-related reductions in Hepatitis B Virus DNA and transaminase levels in patients with chronic HBV hepatitis ...
2013 Training Handout
... Nondisjunction – extra or missing chromosomes as Down’s Syndrome Trinucleotide repeats – triplet nucleotides repeated too often as Huntington’s Defective genes – does not produce correct protein as sickle cell anemia (A & T traded places) Causes of mutations – chemicals, radiation, temperature, viru ...
... Nondisjunction – extra or missing chromosomes as Down’s Syndrome Trinucleotide repeats – triplet nucleotides repeated too often as Huntington’s Defective genes – does not produce correct protein as sickle cell anemia (A & T traded places) Causes of mutations – chemicals, radiation, temperature, viru ...
MCB5472_Lecture_2_Feb-3-14
... read assemblers only use read pairing information AFTER contig assembly during scaffolding – This is starting to change as algorithms ...
... read assemblers only use read pairing information AFTER contig assembly during scaffolding – This is starting to change as algorithms ...
Lecture slides
... As reference one can use an artificial “median array” for a set of arrays or use a log-normal distribution, which is a good approximation. ...
... As reference one can use an artificial “median array” for a set of arrays or use a log-normal distribution, which is a good approximation. ...
DNA Extraction - Utah Agriculture in the Classroom
... This sample could be used for gel electrophoresis, for example, but all you will see is a smear. The DNA you have extracted is genomic, meaning that you have the entire collection of DNA from each cell. Unless you cut the DNA with restriction enzymes, it is too long and stringy to move through the ...
... This sample could be used for gel electrophoresis, for example, but all you will see is a smear. The DNA you have extracted is genomic, meaning that you have the entire collection of DNA from each cell. Unless you cut the DNA with restriction enzymes, it is too long and stringy to move through the ...
DNA - thephysicsteacher.ie
... 27. Understand that TNA bases attach to the unzipped DNA 28. Know that the RNA strand formed in this way is mRNA 29. Know the function of a ribosome in protein synthesis 30. Understand the process of translation that leads to the formation of a new protein 31. Know that the shape of a protein determ ...
... 27. Understand that TNA bases attach to the unzipped DNA 28. Know that the RNA strand formed in this way is mRNA 29. Know the function of a ribosome in protein synthesis 30. Understand the process of translation that leads to the formation of a new protein 31. Know that the shape of a protein determ ...
hiv resistance and treatment failure
... • RNA viruses (which utilize RNA polymerases) mutate faster than retroviruses (which utilize reverse transcriptases), which mutate faster than DNA viruses (which utilize DNA polymerases) ...
... • RNA viruses (which utilize RNA polymerases) mutate faster than retroviruses (which utilize reverse transcriptases), which mutate faster than DNA viruses (which utilize DNA polymerases) ...
Multiple Barriers to Nonhomologous DNA End Joining
... semipermeable barrier) between the sister chromatids that promotes homolog exchange, possibly involving the activity of chromosome axis proteins (Couteau et al. 2004; Webber et al. 2004; Niu et al. 2005; Kim et al. 2010; Li et al. 2011). Finally, homologous repair must be promoted while more error-p ...
... semipermeable barrier) between the sister chromatids that promotes homolog exchange, possibly involving the activity of chromosome axis proteins (Couteau et al. 2004; Webber et al. 2004; Niu et al. 2005; Kim et al. 2010; Li et al. 2011). Finally, homologous repair must be promoted while more error-p ...
Comparison of Deoxyribonucleic Acid Homologies of Six Strains of
... A more extensive survey involving several more strains from each of the three morphological types of Nitrosornonas europaea is needed to determine whether each type consists of a genetically homologous cluster or whether each of the three morphological types can be further separated into additional ...
... A more extensive survey involving several more strains from each of the three morphological types of Nitrosornonas europaea is needed to determine whether each type consists of a genetically homologous cluster or whether each of the three morphological types can be further separated into additional ...
Comparison of Deoxyribonucleic Acid Homologies of Six Strains of
... A more extensive survey involving several more strains from each of the three morphological types of Nitrosornonas europaea is needed to determine whether each type consists of a genetically homologous cluster or whether each of the three morphological types can be further separated into additional ...
... A more extensive survey involving several more strains from each of the three morphological types of Nitrosornonas europaea is needed to determine whether each type consists of a genetically homologous cluster or whether each of the three morphological types can be further separated into additional ...
A single oligonucleotide can be used to rapidly isolate DNA
... adjacent to both ends of a chromosomal Tn5 insertion. Using a single primer extending outward from the ends of the inverted repeat of the transposon, we amplified a probe consisting of DNA sequences of the entire target EcoRI fragment by PCR. Knowing the approximate size of this target fragment, the ...
... adjacent to both ends of a chromosomal Tn5 insertion. Using a single primer extending outward from the ends of the inverted repeat of the transposon, we amplified a probe consisting of DNA sequences of the entire target EcoRI fragment by PCR. Knowing the approximate size of this target fragment, the ...
standard set 5 - EDHSGreenSea.net
... and effectively opened the current era of Molecular Biology. 2. Cells contain two types of nucleic acids: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The main difference between the two is surprisingly simple; RNA lacks a single oxygen atom in a repeating sugar group that forms part of t ...
... and effectively opened the current era of Molecular Biology. 2. Cells contain two types of nucleic acids: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The main difference between the two is surprisingly simple; RNA lacks a single oxygen atom in a repeating sugar group that forms part of t ...
Visualization of oligonucleotide probes and point mutations in
... luorescence in situ hybridization (FISH) techniques are becoming increasingly powerful analytical tools in both basic science and clinical diagnostics (1, 2). The ability to detect aneuploidy, loss of heterozygosity, chromosomal translocations, or abnormal gene expression levels within cytological s ...
... luorescence in situ hybridization (FISH) techniques are becoming increasingly powerful analytical tools in both basic science and clinical diagnostics (1, 2). The ability to detect aneuploidy, loss of heterozygosity, chromosomal translocations, or abnormal gene expression levels within cytological s ...
Powerpoint
... ß-strands. Two heterodimers fit together with formation of a 12-stranded ß-sheet that is sandwiched by a helices. N and C termini of the small and large protease subunits are indicated ...
... ß-strands. Two heterodimers fit together with formation of a 12-stranded ß-sheet that is sandwiched by a helices. N and C termini of the small and large protease subunits are indicated ...
nuclear receptors - SBI
... activated intracelluar transcription factors which up or down regulate the expression of several genes. • Nuclear receptors are soluble proteins that can bind to specific DNA regulatory elements (response elements or REs) and act as cell typeand promoter-specific regulators of transcription. • In co ...
... activated intracelluar transcription factors which up or down regulate the expression of several genes. • Nuclear receptors are soluble proteins that can bind to specific DNA regulatory elements (response elements or REs) and act as cell typeand promoter-specific regulators of transcription. • In co ...
Nuclear Genome Sequence Survey of the
... as dinoflagellate ‘‘genes.’’ One 2,656-bp clone encodes a fragment of a formate/nitrite transporter, several copies of which were identified in the H. triquetra EST survey (Patron et al. 2005), and also found in Prorocentrum minimum (GenBank Accession ABI14400). The genomic copy is not complete: the ...
... as dinoflagellate ‘‘genes.’’ One 2,656-bp clone encodes a fragment of a formate/nitrite transporter, several copies of which were identified in the H. triquetra EST survey (Patron et al. 2005), and also found in Prorocentrum minimum (GenBank Accession ABI14400). The genomic copy is not complete: the ...
Diversity of Endonuclease V: From DNA Repair to RNA Editing
... These two pathways might be employed based on the cellular conditions or external environment such as exposure to nitric oxide or S phase DNA replication. For instance, while DNA glycosylases might repair the deoxyinosines on dsDNA in the G2/M phase, mammalian EndoV may act on deoxyinosines in the s ...
... These two pathways might be employed based on the cellular conditions or external environment such as exposure to nitric oxide or S phase DNA replication. For instance, while DNA glycosylases might repair the deoxyinosines on dsDNA in the G2/M phase, mammalian EndoV may act on deoxyinosines in the s ...
Specialized techniques for site-directed mutagenesis in cyanobacteria
... can be directed to a particular locus. The simplest application is insertional inactivation of a gene, by replacing the gene of interest with an allele that has a heterologous cassette (generally an antibiotic-resistance cassette) inserted within its open reading frame. However, as prokaryotic organ ...
... can be directed to a particular locus. The simplest application is insertional inactivation of a gene, by replacing the gene of interest with an allele that has a heterologous cassette (generally an antibiotic-resistance cassette) inserted within its open reading frame. However, as prokaryotic organ ...
Van, C., Williams, J.S., Kunkel, T.A., and
... monitoring the frequency of 5-FOA or canavanine resistance, respectively. Resistance to 5FOA in the pol3-L612M swr1Δ double mutant strain was 2-fold higher than for the pol3L612M single mutant strain (Fig. 1A). This difference is significant as indicated by no overlap in the 95% confidence intervals ...
... monitoring the frequency of 5-FOA or canavanine resistance, respectively. Resistance to 5FOA in the pol3-L612M swr1Δ double mutant strain was 2-fold higher than for the pol3L612M single mutant strain (Fig. 1A). This difference is significant as indicated by no overlap in the 95% confidence intervals ...
A novel de novo frame-shift mutation of the EDA gene in a
... causes the disease in the family, and it represents the first de novo insertion identified in the EDA gene. The ectodysplasin-A protein contains several domains: a small N-terminal intracellular domain followed by the transmembrane domain, and a larger C-terminal extracellular domain, which contains ...
... causes the disease in the family, and it represents the first de novo insertion identified in the EDA gene. The ectodysplasin-A protein contains several domains: a small N-terminal intracellular domain followed by the transmembrane domain, and a larger C-terminal extracellular domain, which contains ...
The amdR product and a CCAAT-binding factor
... shift assays with crude nuclear extracts we show here that the product of one of these regulatory genes, the amdR gene, binds to DNA fragments containing part of the promoter region of the amdS gene. This confirms the earlier prediction from DNA sequence data that amdR encodes a DNA-binding protein ...
... shift assays with crude nuclear extracts we show here that the product of one of these regulatory genes, the amdR gene, binds to DNA fragments containing part of the promoter region of the amdS gene. This confirms the earlier prediction from DNA sequence data that amdR encodes a DNA-binding protein ...
DNA Testing Procedures - American Hereford Association
... ALLELE: Different forms or variants of a gene are known as alleles. Each animal inherits two alleles of each gene, one from its sire and one from its dam. These two alleles can be identical (making the animal homozygous), or different (making the animal heterozygous) for any given gene. DNA: Deoxyri ...
... ALLELE: Different forms or variants of a gene are known as alleles. Each animal inherits two alleles of each gene, one from its sire and one from its dam. These two alleles can be identical (making the animal homozygous), or different (making the animal heterozygous) for any given gene. DNA: Deoxyri ...
Pre-lab Homework Lab 3: DNA Structure and Function
... Gel Electrophoresis- a technique used for the separation of molecules using an electric field applied to a gel matrix. Molecules can be separated based on their size and their electrical charge. Gel electrophoresis is a commonly used technique in modern biology. It relies on the fact that charged mo ...
... Gel Electrophoresis- a technique used for the separation of molecules using an electric field applied to a gel matrix. Molecules can be separated based on their size and their electrical charge. Gel electrophoresis is a commonly used technique in modern biology. It relies on the fact that charged mo ...